請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68590完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Yi-Ming Chen | en |
| dc.contributor.author | 陳一銘 | zh_TW |
| dc.date.accessioned | 2021-06-17T02:26:32Z | - |
| dc.date.available | 2020-10-19 | |
| dc.date.copyright | 2017-08-28 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-08-18 | |
| dc.identifier.citation | [1] Eggins, B.R., 'Chemical sensors and biosensors'. Vol. 28. John Wiley & Sons. 2008
[2] Löfås, S. and Johnsson, B., 'A novel hydrogel matrix on gold surfaces in surface plasmon resonance sensors for fast and efficient covalent immobilization of ligands', Journal of the Chemical Society, Chemical Communications, 1990. (21): p. 1526-1528. [3] Johnsson, B., et al., 'Immobilization of proteins to a carboxymethyldextran-modified gold surface for biospecific interaction analysis in surface plasmon resonance sensors', Analytical biochemistry, 1991. 198(2): p. 268-277. [4] Daza Millone, M.A., et al., 'SPR Biosensing MUA/Poly-L-lysine Platform for the Detection of 2,4-Dinitrophenol as Small Molecule Model System', Journal of Nanomaterials, 2016. 2016: p. 1-9. [5] Jeon, B.-J., et al., 'Application of a functionalized parylene film as a linker layer of SPR biosensor', Sensors and Actuators B: Chemical, 2011. 154(2): p. 89-95. [6] Chen, H.-Y., et al., 'Colloids with high-definition surface structures', Proceedings of the National Academy of Sciences, 2007. 104(27): p. 11173-11178. [7] Chen, H.Y. and Lahann, J., 'Designable biointerfaces using vapor-based reactive polymers', Langmuir, 2011. 27(1): p. 34-48. [8] Wu, J.T., et al., 'Reactive polymer coatings: a general route to thiol-ene and thiol-yne click reactions', Macromolecular Rapid Communications, 2012. 33(10): p. 922-7. [9] Chen, H.-Y., et al., 'Vapor-based tri-functional coatings', Chemical Communications, 2013. 49(40): p. 4531-3. [10] Tsai, M.-Y., et al., 'Vapor-Based Multicomponent Coatings for Antifouling and Biofunctional Synergic Modifications', Advanced Functional Materials, 2014. 24(16): p. 2281-2287. [11] Hsieh, S.C., et al., 'Rapid identification of Mycobacterium tuberculosis infection by a new array format-based surface plasmon resonance method', Nanoscale Research Letters, 2012. 7(1): p. 180. [12] Hsu, S.H., et al., 'Mycobacterium tuberculosis DNA detection using surface plasmon resonance modulated by telecommunication wavelength', Sensors (Basel), 2013. 14(1): p. 458-67. [13] Barlen, B., et al., 'Detection of Salmonella by Surface Plasmon Resonance', Sensors, 2007. 7(8): p. 1427-1446. [14] Lee, K.-B., et al., 'The use of nanoarrays for highly sensitive and selective detection of human immunodeficiency virus type 1 in plasma', Nano Letters, 2004. 4(10): p. 1869-1872. [15] Pyo, H.-B., et al., 'Multichannel Surface Plasmon Resonance Imaging and Analysis of Micropatterned Self-Assembled Monolayers and Protein Affinity Interactions', Langmuir, 2005. 21(1): p. 166-171. [16] Homola, J., 'Electromagnetic Theory of Surface Plasmons'. 2006, Springer. p. 3-44. [17] Wood, R.W., 'On a remarkable case of uneven distribution of light in a diffraction grating spectrum', Proceedings of the Physical Society of London, 1902. 18(1): p. 269. [18] Kretschmann, E. and Raether, H., 'Notizen: radiative decay of non radiative surface plasmons excited by light', Zeitschrift für Naturforschung A, 1968. 23(12): p. 2135-2136. [19] Otto, A., 'Excitation of nonradiative surface plasma waves in silver by the method of frustrated total reflection', Zeitschrift für Physik A Hadrons and nuclei, 1968. 216(4): p. 398-410. [20] Nelson, S., et al., 'High sensitivity surface plasmon resonace sensor based on phase detection', Sensors and Actuators B: Chemical, 1996. 35(1-3): p. 187-191. [21] Shalabney, A. and Abdulhalim, I., 'Sensitivity-enhancement methods for surface plasmon sensors', Laser & Photonics Reviews, 2011. 5(4): p. 571-606. [22] Homola, J., et al., 'Surface plasmon resonance sensors', Sensors and Actuators B: Chemical, 1999. 54(1): p. 3-15. [23] Love, J.C., et al., 'Self-assembled monolayers of thiolates on metals as a form of nanotechnology', Chemical reviews, 2005. 105(4): p. 1103-1170. [24] Green, R.J., Frazier, R. A., Shakesheff, K. M., Davies, M. C., Roberts, C. J., & Tendler, S. J., 'Surface plasmon resonance analysis of dynamic biological interaction with biomaterials', Biomaterials, 2000. 21(18): p. 182-1835. [25] Ferretti, S., et al., 'Self-assembled monolayers: a versatile tool for the formulation of bio-surfaces', TrAC Trends in Analytical Chemistry, 2000. 19(9): p. 530-540. [26] Sigal, G.B., et al., 'Using surface plasmon resonance spectroscopy to measure the association of detergents with self-assembled monolayers of hexadecanethiolate on gold', Langmuir, 1997. 13(10): p. 2749-2755. [27] Silin, V., et al., 'SPR studies of the nonspecific adsorption kinetics of human IgG and BSA on gold surfaces modified by self-assembled monolayers (SAMs)', Journal of colloid and interface science, 1997. 185(1): p. 94-103. [28] Huber, W., et al., 'Determination of Kinetic Constants for the Interaction Between the Platelet Glycoprotein IIb-IIIa and Fibrinogen by Means of Surface Plasmon Resonance', European Journal of Biochemistry, 2008. 227(3): p. 647-656. [29] Green, R., et al., 'Investigation of the hydration kinetics of novel poly (ethylene oxide) containing polyurethanes', Langmuir, 2000. 16(6): p. 2744-2750. [30] Peterlinz, K.A. and Georgiadis, R., 'In situ kinetics of self-assembly by surface plasmon resonance spectroscopy', Langmuir, 1996. 12(20): p. 4731-4740. [31] Löfås, S. and McWhirter, A., 'The Art of Immobilization for SPR Sensors'. 2006, Springer. p. 117-151. [32] Mrksich, M., et al., 'Surface plasmon resonance permits in situ measurement of protein adsorption on self-assembled monolayers of alkanethiolates on gold', Langmuir, 1995. 11(11): p. 4383-4385. [33] Green, R., et al., 'Competitive protein adsorption as observed by surface plasmon resonance', Biomaterials, 1999. 20(4): p. 385-391. [34] Nowinski, A.K., et al., 'Sequence, structure, and function of peptide self-assembled monolayers', Journal of the American Chemical Society, 2012. 134(13): p. 6000-5. [35] Pollack, K.A., et al., 'Hyperbranched fluoropolymer-polydimethylsiloxane-poly(ethylene glycol) cross-linked terpolymer networks designed for marine and biomedical applications: heterogeneous nontoxic antibiofouling surfaces', ACS Applied Materials & Interfaces, 2014. 6(21): p. 19265-74. [36] Dang, Y., et al., 'Biocompatible and antifouling coating of cell membrane phosphorylcholine and mussel catechol modified multi-arm PEGs', Journal of Materials Chemistry B, 2015. 3(11): p. 2350-2361. [37] Folch, A., 'Introduction to BioMEMS'. CRC Press. 2012 [38] Chen, C., et al., 'Gray-scale photolithography using microfluidic photomasks', Proceedings of the National Academy of Sciences, 2003. 100(4): p. 1499-1504. [39] Fernandez, F., et al., 'A label-free and portable multichannel surface plasmon resonance immunosensor for on site analysis of antibiotics in milk samples', Biosensors and Bioelectronics, 2010. 26(4): p. 1231-8. [40] 'Introduction to Digital Micromirror Device (DMD) Technology'. Texas Instruments. [41] 'DLP System Optics'. Texas Instruments. [42] Kolb, H.C., et al., 'Click chemistry: diverse chemical function from a few good reactions', Angewandte Chemie International Edition, 2001. 40(11): p. 2004-2021. [43] Hoyle, C.E., et al., 'Thiol-enes: Chemistry of the past with promise for the future', Journal of Polymer Science Part A: Polymer Chemistry, 2004. 42(21): p. 5301-5338. [44] Hoyle, C.E. and Bowman, C.N., 'Thiol-ene click chemistry', Angewandte Chemie International Edition, 2010. 49(9): p. 1540-73. [45] Choi, Y.H., et al., 'Development of SPR biosensor for the detection of human hepatitis B virus using plasma-treated parylene-N film', Biosensors and Bioelectronics, 2014. 56: p. 286-94. [46] Gao, Y., et al., 'Secondary structure effects on DNA hybridization kinetics: a solution versus surface comparison', Nucleic Acids Research, 2006. 34(11): p. 3370-7. [47] 'Specificatio for UV LED (NCSU276A)'. NICHIA CORPORATION. [48] 'DLP4500NIR (0.45 WXGA Near-Infared DMD)'. Texas Instruments. [49] 'DLP LCR4500 Evaluation Module User Guide'. Texas Instruments. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68590 | - |
| dc.description.abstract | 傳統上，表面電漿子共振生物感測器（Surface Plasmon Resonance biosensor, SPR biosensor）之表面修飾法（Surface functionalization）以硫金單層自組裝膜（Thiol- gold based Self-Assembled Monolayer, Thiol-gold based SAM）以及其衍生之相關技術為主流。在整個領域的大方向上，除了葡聚醣（Dextran）相關研究與應用外，以高分子層作為修飾方法之基材的研究並不多。由於以高分子層作為修飾層具有許多偵測應用上的優勢，本論文希望提出一套以聚對二甲苯（Parylene）作為基底之修飾方法學。本方法以針對硫醇基之探針分子，以紫外光觸發點擊反應來進行表面固定化。結合數位光源處理技術（Digital Light Process, DLP）系統來控制紫外光之曝光樣式，本修飾方法可以在晶片表面進行指定位置之「定址修飾」。
晶片製作部分，我們透過特殊化學氣相沉積系統來進行聚對二甲苯之鍍膜，並透過表面電漿共振角之偏移以及橢圓儀量測來計算膜厚。根據模擬結果，25 nm之膜厚為最佳化之參數。為進行紫外光曝光，我們以DLP4500 0.45 WXGA DMD為核心建構了一套紫外光曝光系統。此系統的曝光面積大小為10×15 mm2，單位面積之紫外光功率為1×10-4 W/ mm2，每個畫面最小切換週期為235 μs，並結合實驗室之表面電漿共振量測裝置進行即時監測。 為驗證修飾之效能，我們選擇了25-mer 核糖核酸探針分子作為修飾之模型。實驗結果顯示，我們可以在反應濃度1 μM 的probe DNA與5 mM的光啟始劑甲醇溶液，體積比例1：1之條件下，在1000秒內，修飾0.00902 RIU之核糖核酸探針分子。相對於，條件最佳化之硫-金修飾反應，反應速度約快8.9倍。我們透過開啟一半的DMD進行曝光修飾，驗證了定址修飾之效果。實驗結果顯示，UV光照射之區域，產生了1°的角度偏移。 論文最後一章，我們也針對未來技術之潛在議題與未來之發展方向，如在同濃度probe DNA條件下較傳統晶片具有更高的修飾量，進行探討。 | zh_TW |
| dc.description.abstract | Surface functionalization of Surface Plasmon Resonance Biosensor (SPR biosensor) is characterized by thiol-gold based self-assembled monolayer. In addition, there is not so many research and application of polymer layer, dextran for example, as a modified method of the substrate. In this study, we propose a method of surface functionalization using parylene as a linker layer, due to its advantages in the detection of applications. We use a probe molecule with thiol group to immobilize the sensing surface by a UV-triggered click reaction and import a digital light processing (DLP) system to control the exposure pattern of ultraviolet light. The modification method can be ‘‘Spatially Selective’’ on the sensing surface.
In the fabrication section, we use a special chemical vapor deposition system to coat parylene and calculate the film thickness through the angle shift of SPR. According to the simulation results, the optimized thickness of the film is 25 nm. For UV exposure, we built a UV exposure system with DLP4500 0.45 WXGA DMD. The exposure area is 10×15 mm2, the UV power per unit area is 1×10-4 W/ mm2, and the minimum frame period is 235 μs. For real-time measurement, we use a SPR device of our laboratory. To verify, we selected a 25-mer probe DNA as a modified model. The results show that we can modify the probe DNA, and create 0.00902 RIU shift in 1000 seconds. The volume ratio of 1 μM probe DNA and 5 mM DMPA methanol solution is 1:1. Our reaction rate is about 8.9 times faster than sulfur-gold reaction and produces 1° angular offset in UV-exposure. In the last chapter of the thesis, we discuss the potential issues of future technology and development, such as in the same concentration of probe DNA conditions, our chips have high performance than the traditional chips. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T02:26:32Z (GMT). No. of bitstreams: 1 ntu-106-R04548037-1.pdf: 15493920 bytes, checksum: 6f6cefba3cae7d99caa1f5d3b03d8089 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
誌謝 ii 摘要 iii Abstract iv 目錄 v 圖目錄 vii 表目錄 ix 第一章 緒論 1 1.1 研究背景 1 1.2 研究動機與貢獻 2 1.2.1 生物感測表面修飾之發展現況 2 1.2.2 多通道生物感測之優勢與限制 4 1.2.3 新型修飾方法之提出 4 1.3 章節架構 6 第二章 基本原理與文獻回顧 7 2.1 表面電漿子共振 7 2.1.1 表面電漿子共振原理與激發方式 7 2.1.2 表面電漿子共振生物感測器 11 2.1.3 表面電漿子共振量測方式 12 2.1.4 生物感測表面修飾方式 15 2.1.5 非特異性吸附 19 2.2 數位光源處理技術 22 2.2.1 數位微型反射鏡特性 22 2.3 點擊反應 23 2.3.1 硫醇-烯反應 23 第三章 研究材料與方法 25 3.1 表面電漿子共振模擬 25 3.1.1 公式推導 25 3.1.2 模擬軟體 27 3.2 晶片製程 28 3.2.1 基材清洗 29 3.2.2 物理氣相蒸鍍 29 3.2.3 化學氣相蒸鍍 31 3.2.4 硫醇-烯化學改質 34 3.3 系統建構 36 3.3.1 光學系統 36 3.3.2 流體通道設計與種類 40 3.3.3 曝光圖案設計與數位微型反射鏡之控制 42 3.3.4 系統邊界參數測量方式 45 3.3.5 量測軟體 48 3.4 量測與數據分析方法 49 3.4.1 角度調變量測方式 49 3.4.2 強度調變量測方式 50 3.4.3 反射光強度的歸一化 50 3.4.4 折射率的歸一化 51 第四章 研究結果與討論 52 4.1 DMD曝光性能與角度極限測試 52 4.2 緩衝液之水對甲醇比例測試 54 4.3 聚對二甲苯厚度對SPR反應之影響 55 4.4 靈敏度與偵測極限 56 4.5 修飾核酸適體 58 4.6 空間選擇性修飾法驗證 60 4.7 聚對二甲苯之蛋白質吸附測試 63 第五章 結論與未來展望 65 參考文獻 66 附錄一 SPR模擬之MATLAB程式碼 70 附錄二 繪製點陣列圖之Python程式碼 72 | |
| dc.language.iso | zh-TW | |
| dc.subject | 數位微型反射鏡 | zh_TW |
| dc.subject | 硫醇-烯反應 | zh_TW |
| dc.subject | 表面電漿子共振 | zh_TW |
| dc.subject | Thiol-ene reaction | en |
| dc.subject | Digital Micromirror device | en |
| dc.subject | Surface plasmon resonance | en |
| dc.title | 以數位微型反射鏡定址硫醇-烯化學改質之適體探針陣列電漿子晶片 | zh_TW |
| dc.title | Spatially Selective Thiol-ene Functionalization of Plasmonic Apta-sensor Array by Digital Micromirror Device | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 陳賢燁(Hsien-Yeh Chen),彭盛裕(Sheng-Yu Peng),楊家銘(Chia-Ming Yang) | |
| dc.subject.keyword | 表面電漿子共振,硫醇-烯反應,數位微型反射鏡, | zh_TW |
| dc.subject.keyword | Surface plasmon resonance,Thiol-ene reaction,Digital Micromirror device, | en |
| dc.relation.page | 75 | |
| dc.identifier.doi | 10.6342/NTU201700786 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-08-19 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-106-1.pdf 未授權公開取用 | 15.13 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。