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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃義侑 | |
dc.contributor.author | Yu-Chen Liu | en |
dc.contributor.author | 劉宇晨 | zh_TW |
dc.date.accessioned | 2021-06-17T02:22:04Z | - |
dc.date.available | 2019-08-25 | |
dc.date.copyright | 2017-08-25 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-19 | |
dc.identifier.citation | [1] Rousso DE, Kim SW. A review of medical and surgical treatment options for androgenetic alopecia. JAMA facial plastic surgery. 2014;16:444-50.
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[13] Rendl M, Polak L, Fuchs E. BMP signaling in dermal papilla cells is required for their hair follicle inductive properties. Journal of Investigative Dermatology. 2008;128:S143-S. [14] Miao Y, Sun YB, Liu BC, Jiang JD, Hu ZQ. Controllable Production of Transplantable Adult Human High-Passage Dermal Papilla Spheroids Using 3D Matrigel Culture. Tissue Eng Pt A. 2014;20:2329-38. [15] Lin BJ, Miao Y, Wang J, Fan ZX, Du LJ, Su YS, et al. Surface Tension Guided Hanging-Drop: Producing Controllable 3D Spheroid of High-Passaged Human Dermal Papilla Cells and Forming Inductive Microtissues for Hair-Follicle Regeneration. ACS applied materials & interfaces. 2016;8:5906-16. [16] Young TH, Lee CY, Chiu HC, Hsu CJ, Lin SJ. Self-assembly of dermal papilla cells into inductive spheroidal microtissues on poly(ethylene-co-vinyl alcohol) membranes for hair follicle regeneration. Biomaterials. 2008;29:3521-30. [17] Hsieh CH, Wang JL, Huang YY. Large-scale cultivation of transplantable dermal papilla cellular aggregates using microfabricated PDMS arrays. Acta biomaterialia. 2011;7:315-24. [18] Alonso L, Fuchs E. The hair cycle. Journal of cell science. 2006;119:391-3. [19] Hwang J, Mehrani T, Millar SE, Morasso MI. Dlx3 is a crucial regulator of hair follicle differentiation and cycling. Development. 2008;135:3149-59. [20] Park GT, Morasso MI. Bone morphogenetic protein-2 (BMP-2) transactivates Dlx3 through Smad1 and Smad4: alternative mode for Dlx3 induction in mouse keratinocytes. Nucleic acids research. 2002;30:515-22. [21] Toivola DM, Baribault H, Magin T, Michie SA, Omary MB. Simple epithelial keratins are dispensable for cytoprotection in two pancreatitis models. American journal of physiology Gastrointestinal and liver physiology. 2000;279:G1343-54. [22] Toivola DM, Ku NO, Ghori N, Lowe AW, Michie SA, Omary MB. Effects of keratin filament disruption on exocrine pancreas-stimulated secretion and susceptibility to injury. Experimental cell research. 2000;255:156-70. [23] Coulombe PA, Omary MB. 'Hard' and 'soft' principles defining the structure, function and regulation of keratin intermediate filaments. Current opinion in cell biology. 2002;14:110-22. [24] Geisler N, Kaufmann E, Weber K. Antiparallel orientation of the two double-stranded coiled-coils in the tetrameric protofilament unit of intermediate filaments. Journal of molecular biology. 1985;182:173-7. [25] Rogers MA, Langbein L, Praetzel-Wunder S, Winter H, Schweizer J. Human hair keratin-associated proteins (KAPs). International review of cytology. 2006;251:209-63. [26] Sierpinski P, Garrett J, Ma J, Apel P, Klorig D, Smith T, et al. The use of keratin biomaterials derived from human hair for the promotion of rapid regeneration of peripheral nerves. Biomaterials. 2008;29:118-28. [27] Hill PS, Apel PJ, Barnwell J, Smith T, Koman LA, Atala A, et al. Repair of peripheral nerve defects in rabbits using keratin hydrogel scaffolds. Tissue engineering Part A. 2011;17:1499-505. [28] Pechter PM, Gil J, Valdes J, Tomic-Canic M, Pastar I, Stojadinovic O, et al. Keratin dressings speed epithelialization of deep partial-thickness wounds. Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society. 2012;20:236-42. [29] Than MP, Smith RA, Hammond C, Kelly R, Marsh C, Maderal AD, et al. Keratin-based Wound Care Products for Treatment of Resistant Vascular Wounds. The Journal of clinical and aesthetic dermatology. 2012;5:31-5. [30] Burnett LR, Rahmany MB, Richter JR, Aboushwareb TA, Eberli D, Ward CL, et al. Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. Biomaterials. 2013;34:2632-40. [31] Aboushwareb T, Eberli D, Ward C, Broda C, Holcomb J, Atala A, et al. A keratin biomaterial gel hemostat derived from human hair: evaluation in a rabbit model of lethal liver injury. Journal of biomedical materials research Part B, Applied biomaterials. 2009;90:45-54. [32] Reichl S, Borrelli M, Geerling G. Keratin films for ocular surface reconstruction. Biomaterials. 2011;32:3375-86. [33] Borrelli M, Reichl S, Feng Y, Schargus M, Schrader S, Geerling G. In vitro characterization and ex vivo surgical evaluation of human hair keratin films in ocular surface reconstruction after sterilization processing. Journal of materials science Materials in medicine. 2013;24:221-30. [34] Ham TR, Lee RT, Han S, Haque S, Vodovotz Y, Gu J, et al. Tunable Keratin Hydrogels for Controlled Erosion and Growth Factor Delivery. Biomacromolecules. 2016;17:225-36. [35] Lee H, Noh K, Lee SC, Kwon IK, Han DW, Lee IS, et al. Human Hair Keratin and Its-Based Biomaterials for Biomedical Applications. Tissue Eng Regen Med. 2014;11:255-65. [36] Park M, Shin HK, Kim BS, Kim MJ, Kim IS, Park BY, et al. Effect of discarded keratin-based biocomposite hydrogels on the wound healing process in vivo. Mat Sci Eng C-Mater. 2015;55:88-94. [37] Burnett LR, Rahmany MB, Richter JR, Aboushwareb TA, Eberli D, Ward CL, et al. Hemostatic properties and the role of cell receptor recognition in human hair keratin protein hydrogels. Biomaterials. 2013;34:2632-40. [38] Vazquez N, Chacon M, Meana A, Menendez-Menendez Y, Ferrero-Gutierrez A, Cereijo-Martin D, et al. Keratin-chitosan membranes as scaffold for tissue engineering of human cornea. Histology and histopathology. 2015;30:813-21. [39] Ham TR, Lee RT, Han S, Haque S, Vodovotz Y, Gu J, et al. Tunable Keratin Hydrogels for Controlled Erosion and Growth Factor Delivery. Biomacromolecules. 2016;17:225-36. [40] Hartrianti P, Ling L, Goh LM, Ow KS, Samsonraj RM, Sow WT, et al. Modulating Mesenchymal Stem Cell Behavior Using Human Hair Keratin-Coated Surfaces. Stem cells international. 2015;2015:752424. [41] Hill P, Brantley H, Van Dyke M. Some properties of keratin biomaterials: Kerateines. Biomaterials. 2010;31:585-93. [42] Reichl S. Films based on human hair keratin as substrates for cell culture and tissue engineering. Biomaterials. 2009;30:6854-66. [43] Wu YL, Lin CW, Cheng NC, Yang KC, Yu J. Modulation of keratin in adhesion, proliferation, adipogenic, and osteogenic differentiation of porcine adipose-derived stem cells. Journal of biomedical materials research Part B, Applied biomaterials. 2015 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68469 | - |
dc.description.abstract | 禿髮為一種常見的病症,雖然禿髮不會威脅到生命,但對病人的社交活動以及心理健康都會造成深遠的影響。其中雄性禿是造成落髮最常見的原因,由於遺傳基因以及雄性荷爾蒙的影響,造成毛囊萎縮。現行治療方法包含藥物治療或者是植髮。目前美國食品藥物管理局認可的療法包含口服的藥物finasteride以及外用的生髮水minoxidil。在毛囊重建研究中,真皮乳頭細胞是最早被探討以及大量研究的,現已有大量的研究證實真皮乳頭細胞不論在體內或體外培養,皆有誘導毛囊再生的能力。然而隨著繼代培養後,其誘導能力會漸漸消失。
頭髮的角蛋白是一種細胞骨架蛋白,在頭髮生成的周期中,由毛囊內的表皮細胞在頭髮的生長期中生成角蛋白為基礎的材料被應用在生醫領域的包括周邊神經的再生、傷口敷料、止血劑,角膜的重建以及藥物釋放的載體等等。本研究旨在探討利用角蛋白所構成之水膠以及薄膜是否能使真皮乳頭細胞在高代的培養下還能維持其誘導能力。 利用氧化法及還原法能成功的從頭髮萃取出角蛋白keratose 及kerateine,並利用genipin為交聯劑,成功製備出水膠以及薄膜的型態。實驗結果顯示,真皮乳頭細胞在角蛋白材料上有別於培養皿培養的培養狀態,並利用培養皿一半未修飾、一半披覆角蛋白水膠確認此結果是由材料與細胞之間的交互作用所造成的,透過免疫螢光的染色,確認在其上培養能保有其誘導能力。真皮乳頭細胞在keratose水膠上的幹細胞特性遠遠大於在培養皿中培養,或是在明膠水膠上培養。應用此材料於未來體外大量培養頭髮的真皮乳頭細胞應該很有發展的潛力。 | zh_TW |
dc.description.abstract | Alopecia is a common disease that would make huge impact on patients’ mental health and social life. Among all the causes, androgenetic alopecia is the most common cause of alopecia which caused by genetic issue and excessive androgen, resulting in the atrophy of hair follicle. Current treatment includes medicine or hair transplant. The only two US Food and Drug Administration certified therapies include oral administration of finasteride as well as topical hair water minoxidil. In the study of regeneration of hair follicle, dermal papilla were first explored and extensively studied.. It is proved that both in vitro and in vivo culturing have the ability to induce hair follicle regeneration. Unfortunately. After subculture, the ability disappear.
Hair keratin is a kind of cytoskeleton that be made during the hair cycle. It is made by the epithelial cell in the hair follicle in anangen. The keratin based material can be used in the biomedical research, including the regeneration of periphery nerve system, wound healing, hemostat, cornea restruction and drug delivery system. This study aims to investigate whether keratin-based material could maintain the ability of inducing hair follicle regeneration of dermal papilla. We successfully extracted keratose and kerateine by oxidization and reduction method. Using genipin as cross linker, we were able to make film and hydrogel based on keratin. According to the results, dermal papilla cells show different morphology on keratose hydrogel when comparing with normal culture plate. By coating half keratose film on the culture plate, we confirm that the different result of culturing dermal papilla cells was caused by the interaction between the cells and the materials. By immmunostaining, we confirm that culturing cells on keratose hydrogel were able to maintain its ability to induce hair follicle regeneration. Culturing dermal papilla cells on keratose hydrogel could maintain its stemness over culturing on normal tissue plate or on gelatin hydrogel. The material has potential for culturing large quantity dermal papilla cells in vitro. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T02:22:04Z (GMT). No. of bitstreams: 1 ntu-106-R03548066-1.pdf: 2488300 bytes, checksum: 152a53f23db94d84414e36e3ca12f250 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 摘要 ....................................................i
ABSTRACT ..................................................ii 目錄 ...................................................iv 圖目錄 ..................................................vii 表目錄 ...................................................ix 第一章 序論 ............................................1 1.1 禿髮以及其治療 ....................................1 1.1.1 禿髮 ............................................1 1.1.2 禿髮的治療 ....................................1 1.2 毛囊簡介 ............................................2 1.2.1 毛囊結構 ............................................2 1.2.2 毛囊的型態發育學 ....................................2 1.2.3 毛髮週期 ............................................3 1.3 毛囊的重建 ....................................4 1.3.1 與角質細胞共培養 ....................................4 1.3.2 與會表現Wnt的細胞共培養 ............................5 1.3.3 三維培養環境 ....................................5 1.4 人類頭髮角蛋白簡介 ............................5 1.4.1. 人類頭髮角蛋白的應用 ............................7 1.5 人類頭髮角蛋白於細胞培養 ...........................10 1.6 水膠 ...........................................11 第二章 研究概述 ...........................................12 第三章 實驗材料與方法 ...................................13 3.1 實驗藥品 ...........................................13 3.2 實驗儀器 ...........................................15 3.3 角蛋白萃取 ...................................16 3.3.1 氧化法-Keratose ...................................17 3.3.2 還原法-Kerateine ...................................17 3.4 SDS-PAGE以及Coomassie blue R-250染色 ...........17 3.5 細胞毒性測試 ...................................20 3.5.1 NIH/3T3繼代培養 ...................................20 3.5.2 MTT assay ...................................21 3.6 水膠製備 ...........................................22 3.6.1 Keratose hydrogel ...........................22 3.6.2 Kerateine hydrogel ...........................22 3.7 真皮乳頭細胞分離與培養 ...........................22 3.8 培養真皮乳頭細胞於水膠上 ...........................23 3.9 真皮乳頭細胞於未修飾及修飾keratose薄膜上之培養 ...23 3.10 培養真皮乳頭細胞於豬明膠薄膜與水膠上 ...........23 3.11 觀察keratose溶於培養基對培養真皮乳頭細胞之影響 ...24 3.12 免疫螢光染色 ...................................24 第四章 實驗結果與討論 ...................................26 4.1 SDS-PAGE ...................................26 4.2 細胞毒性 ...........................................26 4.3 培養真皮乳頭細胞於水膠上 ...........................27 4.3.1 Keratose hydrogel ...........................27 4.3.2 Kerateine hydrogel ...........................27 4.4 培養真皮乳頭細胞於一半的keratose薄膜上 ...........29 4.5 培養真皮乳頭細胞於豬明膠薄膜與水膠上 ...........30 4.6 觀察keratose溶於培養基對培養真皮乳頭細胞之影響 ...30 4.7 薄膜上形成之細胞團塊活性 ...........................31 4.8 免疫螢光染色 ...................................31 參考文獻 ...................................................37 | |
dc.language.iso | zh-TW | |
dc.title | 以角蛋白水膠培養真皮乳頭細胞 | zh_TW |
dc.title | Culturing dermal papilla cells on keratin-based hydrogels | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃意真,許馨云 | |
dc.subject.keyword | 角蛋白,水膠,真皮乳頭細胞,交聯劑,細胞成團, | zh_TW |
dc.subject.keyword | keratin,hydrogel,dermal papilla,cross linker,cell sphere, | en |
dc.relation.page | 40 | |
dc.identifier.doi | 10.6342/NTU201703653 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-08-20 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
顯示於系所單位: | 醫學工程學研究所 |
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