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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68405
標題: | 結合微機電掃描技術與光學同調斷層掃描術以及血管攝影術於鼠腦成像技術開發 Development of optical coherence tomography (OCT) and OCT angiography imaging of the mouse brain in vivo with microelectromechanical system (MEMS) scanning technologies |
作者: | Yu-Wei Chang 張育瑋 |
指導教授: | 李翔傑(Hsiang-Chieh Lee) |
關鍵字: | 光學同調斷層掃描術,血管照影術,小型化OCT系統,微機電掃描技術,活體動物腦部成像,神經科學, optical coherence tomography,OCT angiography,miniature OCT system,microelectromechanical system scanning technologies,in vivo small animal brain imaging,neuroscience, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 在傳統的光學同調斷層掃描 (optical coherence tomography, OCT)對於小鼠神經科學的研究中,由於系統體積及重量太大,因此只能將小鼠固定在載台上並將其麻醉,這種做法使得實驗只能獲得麻醉狀態下的鼠腦資訊,而且限制了OCT 在神經科學上的發展。因此本篇論文中提出了一種小型頭戴式OCT系統(Miniature Head-mounted OCT, MH-OCT),將OCT系統小型化並減經它的重量,使我們能順利戴在自由活動的小鼠頭上,並利用血管照影術 (OCT Angiography, OCTA)獲取腦部血管影像。該系統使用的是中心波長1310奈米、掃描頻率400 kHz的掃頻式OCT系統(Swept-source OCT, SS-OCT),並結合了微機電掃描技術,使其能夠快速地獲取三維OCTA影像。我們在實驗中設定了三個不同的小鼠生理狀態(麻醉、清醒、活動),並使用MH-OCT進行血管照影,比較三種生理狀態下的腦部活動差異。實驗結果表明,在不同生理狀態下,腦部血管會有管徑及流量上的變化,也進一步證實OCT可利用在腦部行為科學的研究上。 The conventional optical coherence tomography (OCT) instrument setup is not suitable for imaging in freely behaving animal because the system is huge and heavy. These disadvantages limit the broader implementations of OCT in neuroscience because researchers only can perform brain imaging of the animal, either under anesthesia or holding the brain in a fixed position. Therefore, in this thesis work, we have developed the miniature head-mounted OCT (MH-OCT) imaging device allowing continuous imaging of the mouse brain in a freely moving state. This device utilizes the micro-electro-mechanical system (MEMS) scanning technologies and high-speed wavelength-swept light source to enable fast volumetric of the mouse brain with a lightweight footprint. To observe the relationship between the brain activity and associated physiological conditions, we used the OCT angiography (OCTA) to obtain the microvasculature information of the mouse brain under three different physiological conditions, including anesthesia, awake, and movement. The preliminary result demonstrates that the brian microvasculature changes in diameter and flow under different physiological conditions, suggesting that OCT can be used in the research of behavioral brain sciences. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68405 |
DOI: | 10.6342/NTU202003743 |
全文授權: | 有償授權 |
顯示於系所單位: | 光電工程學研究所 |
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