請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68236
標題: | 利用微流體乳化與電控微流體技術建構與排列肝細胞水膠組織 Constructing and Manipulating Cell-Laden Hydrogels with Microfluidic Emulsion and Electromicrofluidic Technologies |
作者: | Jia-Tai Liao 廖家泰 |
指導教授: | 范士岡(Shih-Kang Fan) |
關鍵字: | 微流道,電控微流體平台,肝臟小球,水膠材料,組織工程, microchannel,electromicrofluidic platform,hepatocyte spheroids,hydrogel,tissue engineering, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 由於無法精準控制細胞排列,現今組織工程技術不易建構出真實生物體內之組織。本研究欲利用微流體乳化技術以及電控微流體技術建構出模擬生物體內細胞生長之三維異質細胞組織。實驗方法主要包括:(1)微流體乳化技術:切分含肝臟細胞的水膠預聚物溶液,形成微米等級的乳化液滴;(2)電場細胞排列技術:將乳化液滴中肝臟細胞適當排列並固化水膠後,得到肝臟小球組織;(3)利用電控微流體技術操控肝臟小球組織於另一水膠預聚物溶液。此技術展現多種材料、多種肝臟細胞、與不同尺度的操控,具有實現體外組織工程之潛力。實驗使用材料為具有生物相容性之水膠材料,包含poly (ethylene glycol) diacrylate (PEGDA)以及Gelatin methacryloyl (GelMA),水膠在摻入光起始劑I-2959之後接具有光交聯性質,透過紫外光照射後水膠材料15 s內會由液態轉變成為固態或膠態,利用此材料特性使得微流道系統得以將其液體時切分為乳化液滴,並且在交聯後使用電控微流體平台,在低導電緩衝溶液環境中操縱及建構細胞組織,操縱電壓為20-40 V。本研究將探討多種不同水相溶液、油相溶液、介面活性劑和濃度,實驗各項材料性質並深入了解其運作原理。最後成功利用微流體乳化技術以及電控微流體技術,來展現微小尺度下的細胞操控及組織建構能力。 Constructing biomimicking tissues is only possible when the arrangements of cells are precisely performed. In this study, we use microfluidic emulsion and electromicrofluidic technologies to construct three-dimensional heterogeneous cell tissues that simulate the configurations of cells in vivo. In our experiments, (1) a variety of hydrogels and cell-containing hydrogel prepolymer solutions were prepared to produce and hydrogel spheroids by microfluidic emulsion. (2) The cells in the artificial tissues were arranged by electromicrofluidic techniques, and (3) the cell spheroids were cured by ultraviolet light. The spheroids were manipulated on the electromicrofluidic platform to achieve cross-scale manipulations and in vitro tissue engineering. The biocompatible hydrogel materials used here were poly (ethylene glycol) diacrylate (PEGDA) and Gelatin methacryloyl (GelMA). The hydrogel is photo-crosslinkable with photoinitiator I-2959; the hydrogel transformed from liquid into solid or colloidal state with UV light exposed for 15 s. With these characteristics, hydrogel solution can be dripped into hydrogel spheroids by microfluidic emulsion technique and manipulated in a low-conductivity buffer solution environment with a voltage of 20-40 V by electromicrofluidic technique. We explore a variety of different aqueous solutions, oil solutions, surfactants at varied concentrations to test the material properties. Hepatocyte spheroids were obtained to demonstrate the cell manipulations on micrometer scale and tissue constructions. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68236 |
DOI: | 10.6342/NTU201704294 |
全文授權: | 有償授權 |
顯示於系所單位: | 機械工程學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 6.4 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。