請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68162
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃升龍(Sheng-Lung Huang) | |
dc.contributor.author | Jheng-Yu Wu | en |
dc.contributor.author | 吳政育 | zh_TW |
dc.date.accessioned | 2021-06-17T02:13:47Z | - |
dc.date.available | 2021-01-04 | |
dc.date.copyright | 2018-01-04 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-11-27 | |
dc.identifier.citation | [1] C. A. Patil, H. Kirshnamoorthi, D. L. Ellis et al., “A clinical instrument for combined Raman spectroscopy-optical coherence tomography of skin cancers,” Lasers in Surgery and Medicine, vol. 43, no. 2, pp. 143–151, Feb. 2011.
[2] “Skin cancer fact sheet.” American Cancer Society, 2017. [3] C.-H. Liu, J. Qi, J. Lu et al., “Improvement of tissue analysis and classification using optical coherence tomography combined with Raman spectroscopy,” in Proc. SPIE 8942, Dynamics and Fluctuations in Biomedical Photonics XI, vol. 8942, no. 4, p. 894208, 2014. [4] J. W. Evans, R. J. Zawadzki, R. Liu et al., “Optical coherence tomography and Raman spectroscopy of the ex-vivo retina,” Journal of Biophotonics, vol. 2, no. 6–7, pp. 398–406, Jul. 2009. [5] V. Klein and T. E. Furtak, Optics. Wiley New York, 1990. [6] 吳東憶, “高解析且高深度Mirau全域式光學同調斷層掃描之活體皮膚量測,” 國立臺灣大學, 2015. [7] “The Rayleigh criterion.” [Online]. Available: http://hyperphysics.phy-astr.gsu.edu/hbase/phyopt/Raylei.html. [8] A. Dubois and A. C. Boccara, Optical coherence tomography: Technology and applications. Springer, 2008. [9] 王政凱, “摻鈦藍寶石寬頻晶體光纖光源之製備與檢測,” 國立臺灣大學, 2011. [10] Y. Dong, G. Zhou, X. Jun et al., “Luminescence studies of Ce:YAG using vacuum ultraviolet synchrotron radiation,” Materials Research Bulletin, vol. 41, no. 10, pp. 1959–1963, 2006. [11] “Molecular motions.” [Online]. Available: https://2012books.lardbucket.org/books/principles-of-general-chemistry-v1.0/s22-04-entropy-changes-and-the-third-.html. [12] Y. Sawai, B. Takimoto, H. Nabika et al., “Anti-Stokes/Stokes ratio of surface-enhanced Raman scattering spectra observed at a metal nano-gap arrayed on a solid surface,” Canadian Journal of Analytical Sciences and Spectroscopy, vol. 52, no. 3, pp. 142–149, 2007. [13] K. Venkateswarlu, “Relative intensities of Stokes and anti-Stokes Raman lines in crystals,” in Proc. The Indian Academy of Sciences, 1940, pp. 64–67. [14] R. S. Krishnan and P. S. Narayanan, “Intensity ratio of the Raman lines in diamond,” in Proc. The Indian Academy of Sciences-mathematical Sciences, 1950. [15] I. H. Boyaci, H. E. Genis, B. Guven et al., “A novel method for quantification of ethanol and methanol in distilled alcoholic beverages using Raman spectroscopy,” Journal of Raman Spectroscopy, vol. 43, no. 8, pp. 1171–1176, Aug. 2012. [16] E. J. Woodbury and W. K. Ng, “Ruby laser operation in the near IR,” 1962. [17] V. V. Yakovlev, G. I. Petrov, H. F. Zhang et al., “Stimulated Raman scattering: Old physics, new applications,” Journal of Modern Optics, vol. 56, no. 18–19, pp. 1970–1973, 2009. [18] C. W. Freudiger, W. Min, B. G. Saar et al., “Label-free biomedical imaging with high sensitivity by stimulated Raman scattering microscopy,” Science, vol. 322, no. 5909, pp. 1857–1861, Dec. 2008. [19] “受激拉曼散射.” [Online]. Available: https://en.wikipedia.org/wiki/Raman_scattering#Stimulated_Raman_scattering_and_Raman_amplification. [20] 江宛霖, “以寬頻種子光源做激發拉曼散射增強之研究,” 國立臺灣大學, 2015. [21] F. Lu, W. Zheng, C. Sheppard et al., “Interferometric polarization coherent anti-Stokes Raman scattering (IP-CARS) microscopy,” Optics Letters, vol. 33, no. 6, p. 602, Mar. 2008. [22] F. Lu, W. Zheng, and Z. Huang, “Heterodyne polarization coherent anti-Stokes Raman scattering microscopy,” Applied Physics Letters, vol. 92, no. 12, p. 123901, Mar. 2008. [23] F. Lu, W. Zheng, J. Lin et al., “Integrated coherent anti-Stokes Raman scattering and multiphoton microscopy for biomolecular imaging using spectral filtering of a femtosecond laser,” Applied Physics Letters, vol. 96, no. 13, p. 133701, Mar. 2010. [24] F. Lu, “Development of high contrast coherent anti-Stokes Raman scattering (CARS) and multiphoton microscopy for label-free biomolecular imaging,” National University of Singapore, 2010. [25] Y. Ozeki and K. Itoh, “Stimulated Raman scattering microscopy for live-cell imaging with high contrast and high sensitivity,” Laser Physics, vol. 20, no. 5, pp. 1114–1118, May 2010. [26] M. Lee, W. J. Tipping, A. Serrels et al., “New approaches to intracellular drug imaging by stimulated Raman scattering microscopy,” in Proc. SPIE 10069, Multiphoton Microscopy in the Biomedical Sciences XVII, vol. 10069, p. 100690T, 2017. [27] L. Wei, Y. Yu, Y. Shen et al., “Vibrational imaging of newly synthesized proteins in live cells by stimulated Raman scattering microscopy,” Pnas, vol. 110, no. 28, pp. 11226–11231, 2013. [28] S. Hong, T. Chen, Y. Zhu et al., “Live-cell stimulated Raman scattering imaging of alkyne-tagged biomolecules,” Angewandte Chemie - International Edition, vol. 53, no. 23, pp. 5827–5831, 2014. [29] B. Mallick, A. Lakshmanna, V. Radhalakshmi et al., “Design and development of stimulated Raman spectroscopy apparatus using a femtosecond laser system,” Current Science, vol. 95, no. 11, pp. 1551–1559, 2008. [30] Z. Meng, G. I. Petrov, and V. V. Yakovlev, “Continuous-wave stimulated Raman scattering (cwSRS) microscopy,” Applied Physics B: Lasers and Optics, vol. 112, no. 1, pp. 99–103, 2013. [31] P. Vallée, J. Lafait, M. Ghomi et al., “Raman scattering of water and photoluminescence of pollutants arising from solid-water interaction,” Journal of Molecular Structure, vol. 651–653, pp. 371–379, 2003. [32] S. Burikov, T. Dolenko, S. Patsaeva et al., “Raman and IR spectroscopy research on hydrogen bonding in water-ethanol systems,” Molecular Physics, vol. 108, no. 18, pp. 2427–2436, 2010. [33] Q. Sun, “The Raman OH stretching bands of liquid water,” Vibrational Spectroscopy, vol. 51, no. 2, pp. 213–217, 2009. [34] N. Tschirner, “Raman spectroscopy of β-carotene and CdSe-based nanocrystals,” der Technischen Universit at Berlin, 2012. [35] “Beta-carotene分子結構.” [Online]. Available: https://schoolworkhelper.net/beta-carotene-function-sources/. [36] “皮膚簡介.” [Online]. Available: https://zh.wikipedia.org/wiki/皮膚. [37] “皮膚系統.” [Online]. Available: http://www2.cnsh.mlc.edu.tw/teaching/biology/bio2/13.htm#B46_1B. [38] 劉利生, 常見病治療和預防常識--皮膚病 [39] “皮膚的結構.” [Online]. Available: http://home.sbc.edu.hk/~bio/bioproject/skin/part1.html. [40] “皮膚的生理結構.” [Online]. Available: http://drtonywu.pixnet.net/blog/post/26010100-【醫師專欄-皮膚】-認識皮膚的生理結構-struct. [41] “Skin structure.” [Online]. Available: https://www.earthslab.com/wp-content/uploads/2017/01/011417_1618_CellsAndLay1.jpg. [42] “皮膚構造與功能與保健.” [Online]. Available: http://web2.ctsh.hcc.edu.tw/stu98/s9811206/public_html/work/skin.pdf. [43] “表皮(Epidermis).” [Online]. Available: https://smallcollation.blogspot.tw/2013/09/epidermis.html#gsc.tab=0. [44] “甘油簡介.” [Online]. Available: https://zh.wikipedia.org/wiki/甘油. [45] A. Mudalige and J. E. Pemberton, “Raman spectroscopy of glycerol/D2O solutions,” Vibrational Spectroscopy, vol. 45, no. 1, pp. 27–35, 2007. [46] “角鯊烯特性.” [Online]. Available: http://raywu9999.pixnet.net/blog/post/139966846-角鯊烯%28squalene%29. [47] “角鯊烯簡介.” [Online]. Available: http://b303094004.pixnet.net/blog/post/291251851-角鯊烯,有這麼厲害嗎?. [48] “角鯊烯分子結構.” [Online]. Available: http://www.emdmillipore.com/PR/en/product/Squalene,MDA_CHEM-821068. [49] H. J. Chun, T. L. Weiss, T. P. Devarenne et al., “Vibrational spectra and DFT calculations of squalene,” Journal of Molecular Structure, vol. 1032, pp. 203–206, 2013. [50] “三酸甘油酯分子結構.” [Online]. Available: http://www.urantiagaia.org/eng/vital/timsmith/outsmarting_n1_killer7.html. [51] S. Bresson, M. El Marssi, and B. Khelifa, “Raman spectroscopy investigation of various saturated monoacid triglycerides,” Chemistry and Physics of Lipids, vol. 134, no. 2, pp. 119–129, 2005. [52] L. Hayflick and P. S. Moorhead, “The serial cultivation of human diploid cell strains,” Experimental Cell Research, vol. 25, no. 3, pp. 585–621, 1961. [53] S. Y. F. C. Gallier, “Understanding the structure of the bovine milk fat globule and its membrane by means of microscopic techniques and model systems,” Otago, 2010. [54] I. P. Santos, P. J. Caspers, T. C. Bakker Schut et al., “Raman spectroscopic characterization of melanoma and benign melanocytic lesions suspected of melanoma using high-wavenumber Raman spectroscopy,” Analytical Chemistry, vol. 88, no. 15, pp. 7683–7688, 2016. [55] M. Gniadecka, P. A. Philipsen, S. Sigurdsson et al., “Melanoma diagnosis by Raman spectroscopy and neural networks: Structure alterations in proteins and lipids in intact cancer tissue,” Journal of Investigative Dermatology, vol. 122, no. 2, pp. 443–449, 2004. [56] B. Bodanese, F. L. Silveira, R. A. Zângaro et al., “Discrimination of basal cell carcinoma and melanoma from normal skin biopsies in vitro through Raman spectroscopy and principal component analysis,” Photomedicine and Laser Surgery, vol. 30, no. 7, pp. 381–387, Jul. 2012. [57] L. Silveira, F. L. Silveira, B. Bodanese et al., “Discriminating model for diagnosis of basal cell carcinoma and melanoma in vitro based on the Raman spectra of selected biochemicals,” Journal of Biomedical Optics, vol. 17, no. 7, p. 77003, Jul. 2012. [58] E. Brauchle, S. Noor, E. Holtorf et al., “Raman spectroscopy as an analytical tool for melanoma research,” Clinical and Experimental Dermatology, vol. 39, no. 5, pp. 636–645, Jul. 2014. [59] 簡孟庭, “高解析度Mirau全域式光學同調斷層掃描儀於組織切片之腫瘤辨識研究,” 國立臺灣大學, 2015. [60] S. Fendel and B. Schrader, “Investigation of skin and skin lesions by NIR-FT-Raman spectroscopy,” Fresenius Journal of Analytical Chemistry, vol. 360, no. 5, pp. 609–613, 1998. [61] 林彥宏, “Mirau全域式光學同調斷層掃描於皮膚 結構及動態特性分析,” 國立臺灣大學, 2016. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68162 | - |
dc.description.abstract | 檢測完整的生物組織特性需要樣本的形貌資訊與生物化學資訊,光學同調斷層掃描術(Optical coherence tomography; OCT)擅於呈現樣本組織的立體微結構,但卻缺少生物分子的資訊;拉曼光譜儀(Raman spectroscopy)可以提供組織的生化成分資訊,但卻無法呈現組織的立體微結構資訊。為了能獲得完整的生物樣本資訊,在本論文中,我們將研發能獲得生物樣本立體形貌與生化成分資訊的拉曼光譜儀結合全域式光學同調斷層掃描術系統,並展示此系統應用於in vitro細胞株與ex vivo皮膚組織之量測結果。
在in vitro黑色素癌細胞的量測上,首先使用OCT系統建構出黑色素癌細胞的立體影像與其在空間中的位置,接著再利用此OCT影像引導拉曼光譜儀在細胞的位置上進行拉曼光譜的採集。實驗結果顯示,OCT的立體影像可以忠實地呈現細胞的立體形貌、實際大小(約10–15 μm)以及在空間中的具體位置,此外,拉曼頻譜也確實記錄到黑色素癌細胞的拉曼特徵訊號(1326 cm-1、1469 cm-1、1660 cm-1、2939 cm-1),其拉曼峰值位置與文獻相符。 在ex vivo皮膚組織的量測上,分別對黑色素細胞癌組織蠟塊切片與正常皮膚組織蠟塊切片進行量測與區分,實驗結果顯示,雖然無法單純從兩者的OCT立體影像進行型態鑑別,但是兩者的拉曼頻譜有存在著差異,其差異在於有無位於1326 cm-1的拉曼訊號,因此能利用此拉曼訊號進行黑色素細胞癌組織與正常皮膚組織的辨別。 本論文展現拉曼光譜儀結合全域式光學同調斷層掃描術系統應用於量測生物樣本的潛力,不僅能獲得生物樣本的立體形貌,還能得知樣本所含有的生物分子資訊,讓生物樣本的分析得以更加地完整,以利於醫生對於病灶的診斷。 | zh_TW |
dc.description.abstract | Complete characterization of biological tissue requires probing the morphological and biochemical information. Optical coherence tomography (OCT) excels at presenting three-dimensional (3-D) images of tissue microstructure but lacks biomolecular information, while Raman spectroscopy is capable of providing tissue biochemical composition with superb specificity, but cannot gather 3-D microstructure. In order to obtain the complete biological sample information, in this work, we develop an integrated full-field OCT (FF-OCT) and Raman spectroscopy system capable of acquiring 3-D morphology and biochemical composition from biological samples, and demonstrate the capabilities of this system with in vitro cell line, as well as ex vivo human skin tissue.
In the in vitro melanoma cell line measurement, first, our FF-OCT system is used to construct the 3-D image of melanoma cells and acquire their position in space. Then, we utilize this 3-D image to guide the acquisition of Raman spectrum from a localized melanoma cells. The experimental results show that 3-D OCT images can truly present the 3-D morphology, size (10–15 μm) and specific position in space of cells. Furthermore, the characteristic Raman peaks of melanoma cells (1326 cm-1、1469 cm-1、1660 cm-1、2939 cm-1) are indeed detected by Raman spectroscopy, which is consistent with the results found in literature. In the ex vivo experiment, we measure the melanoma biopsy and normal skin biopsy, and try to discriminate between them by the integrated FF-OCT and Raman spectroscopy system. The experimental results show that it cannot simply distinguish between melanoma biopsy and normal skin biopsy by the OCT image, but there is also a difference in the Raman spectrum at 1326 cm-1, enabling us to differentiate melanoma skin tissue and normal skin tissue by this Raman signal. This work shows the potential of applying integrated FF-OCT and Raman spectroscopy system to biological tissue measurements. By using this system, not only the 3-D tissue microstructure can be obtained, but also the biochemical composition, resulting in a more complete analysis of biological samples, which provides better assistance for the diagnose of diseases. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T02:13:47Z (GMT). No. of bitstreams: 1 ntu-106-R04941031-1.pdf: 3940168 bytes, checksum: 1f995cd0fb1fc81d058b5d1634ca5363 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 致謝 I
摘要 II Abstract III 圖目錄 VII 表目錄 XI 第一章 緒論 1 第二章 全域式光學同調斷層掃描 3 2.1光學同調斷層掃描基本原理 3 2.2 全域式光學同調斷層掃描術 9 2.2.1系統簡介 9 2.2.2 OCT影像處理 10 2.2.3 FF-OCT之橫向、縱向解析度 13 2.3摻鈰釔鋁石榴石晶體光纖寬頻光源 16 2.3.1摻鈰釔鋁石榴石晶體光纖製備 16 2.3.2寬頻光源特性量測 18 2.4 Michelson-based全域式光學同調斷層掃描系統設計與特性量測 21 三 拉曼散射、拉曼系統及皮膚表皮層資訊 28 3.1拉曼散射與拉曼系統 28 3.1.1拉曼散射 28 3.1.2拉曼系統與系統靈敏度 37 3.2皮膚表皮層介紹及脂質成分之拉曼特性 46 3.2.1皮膚功能與皮膚表皮層結構 46 3.2.2皮膚角質層脂質成分的拉曼特性 49 四 拉曼光譜儀結合全域式光學同調斷層掃描術系統 61 4.1 In vitro黑色素癌細胞量測 63 4.1.1樣本製備 63 4.1.2 OCT影像結合拉曼頻譜量測結果 66 4.2 黑色素細胞癌組織與正常皮膚組織蠟塊切片之量測 75 4.2.1樣本製備 75 4.2.2 OCT影像結合拉曼頻譜量測結果 77 第五章 結論與未來展望 86 5.1 結論 86 5.2 未來展望 88 參考資料 91 | |
dc.language.iso | zh-TW | |
dc.title | 全域式光學同調斷層掃描術結合拉曼光譜儀用於生物樣本的特性分析 | zh_TW |
dc.title | Full-field Optical Coherence Tomography Combined with Raman Spectroscopy for Biological Sample Characterization | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 邱政偉(Jeng-Wei Tjiu),吳育弘(Yu-Hung Wu) | |
dc.subject.keyword | 拉曼光譜儀結合全域式光學同調斷層掃描術系統,拉曼頻譜,黑色素細胞癌, | zh_TW |
dc.subject.keyword | Integrated full-field OCT and Raman spectroscopy system,Raman spectrum,melanoma, | en |
dc.relation.page | 97 | |
dc.identifier.doi | 10.6342/NTU201704402 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-11-27 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
顯示於系所單位: | 光電工程學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 3.85 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。