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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 吳克強(Keqiang Wu) | |
| dc.contributor.author | Pei-Yu Lin | en |
| dc.contributor.author | 林珮伃 | zh_TW |
| dc.date.accessioned | 2021-06-17T02:11:54Z | - |
| dc.date.available | 2023-01-29 | |
| dc.date.copyright | 2018-01-29 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2018-01-05 | |
| dc.identifier.citation | Back, K. and Chappell, J. (1996) Identifying functional domains within terpene cyclases using a domain-swapping strategy. Proc. Natl. Acad. Sci. 93(13): 6841—6845.
Bailey, T. L. and Elkan, C. (1994) Fitting a mixture model by expectation maximization to discover motifs in biopolymers. Pages 28—36 in: Proceedings of the Second International Conference on Intelligent Systems for Molecular Biology. American Association for Artificial Intelligence Press, Menlo Park, CA, U.S.A. Bailey, T. L. and Gribskov, M. (1998) Combining evidence using p-values: Application to sequence homology searches. Bioinformatics 14: 48—54. Barger, S. L. (2007) The complex language of chromatin regulation during transcription. Nature 447(7143): 407—412. Bassett, A., Cooper, S., Wu, C. and Travers, A. (2009) The folding and unfolding of eukaryotic chromatin. Curr. Opin. Genet. Dev. 19: 159—165. Cai, M., Qiu, D., Yuan, T., Ding, X., Li, H., Duan, L., Xu, C., Li, X. and Wang, S. (2008) Identification of novel pathogen-responsive cis-elements and their binding proteins in the promoter of OsWRKY13, a gene regulating rice disease resistance. Plant Cell Environ. 31(1): 86—96. Cai, Y., Chen, X., Xie, K., Xing, Q., Wu, Y., Li, J., Du, C., Sun, Z. and Guo, Z. (2014) Dlf1, a WRKY transcription factor, is involved in the control of flowering time and plant height in rice. PLoS One 9(7): e102529. Chen, J., Nolan, T., Ye, H., Zhang, M., Tong, H., Xin, P., Chu, J., Chu, C., Li, Z. and Yin, Y. (2017a). Arabidopsis WRKY46, WRKY54 and WRKY70 transcription factors are involved in brassinosteroid-regulated plant growth and drought response. Plant Cell 29: 1425—1439. Chen, L. F. and Greene, W. C. (2003) Regulation of distinct biological activities of the NF-κB transcription factor complex by acetylation. J. Mol. Med. 81: 549—557. Chen, L. F., Fischle, W., Verdin, E. and Greene, W. C. (2001) Duration of nuclear NF-κB action regulated by reversible acetylation. Science 293: 1653—1657. Chen, L. F., Mu, Y. and Greene, W. C. (2002) Acetylation of RelA at discrete sites regulates distinct nuclear functions of NF-κB. EMBO J. 21: 6539—6548. Chen, L., Xiang, S., Chen, Y., Li, D. and Yu, D. (2017b). Arabidopsis WRKY45 interacts with the DELLA protein RGL1 to positively regulate age-triggered leaf senescence. Mol. Plant 10: 1174—1189. Chi, Y., Yang, Y., Zhou, Y., Zhou, J., Fan, B., Yu, J. Q. and Chen, Z. (2013) Protein-protein interactions in the regulation of WRKY transcription factors. Mol. Plant 6(2): 287—300. Choudhary, C., Weinert, B. T., Nishida, Y., Verdin, E. and Mann, M. (2014) The growing landscape of lysine acetylation links metabolism and cell signaling. Nat. Rev. Mol. Cell Biol. 15: 536—550. Ciolkowski, I., Wanke, D., Birkenbihl, R. P. and Somssich, I. E. (2008) Studies on DNA-binding selectivity of WRKY transcription factors lend structural clues into WRKY-domain function. Plant Mol. Biol. 68:81—92. Eulgem, T., Rushton, P. J., Robatzek, S. and Somssich, I. E. (2000) The WRKY superfamily of plant transcription factors. Trends Plant Sci. 5(5): 199—206. Friso, G. and van Wijk, K. J. (2015) Posttranslational protein modifications in plant metabolism. Plant Physiol. 169: 1469—1487. Fujimoto, S.Y., Ohta, M., Usui, A., Shinshi, H. and Ohme-Takagi, M. (2000) Arabidopsis ethylene-responsive element binding factors act as transcriptional activators or repressors of GCC box-mediated gene expression. Plant Cell 12(3): 390—404. Gengrel, A. V., Lippman, Z., Martienssen, R. and Colot, V. (2005) Profiling histone modification patterns in plants using genomic tiling microarrays. Nat. Methods 2(3): 213—218. Glatt, S., Alfieri, C. and Müller, C. W. (2011) Recognizing and remodeling the nucleosome. Curr. Opin. Struct. Biol. 21: 335—341. Glozak, M. A., Sengupta, N., Zhang, X. and Seto, E. (2005) Acetylation and deacetylation of non-histone proteins. Gene 363: 15—23. Grierson, C., Du, J. S., de Torres Zabala, M., Beggs, K., Smith, C., Holdsworth, M. and Bevan, M. (1994) Separate cis sequences and trans factors direct metabolic and developmental regulation of a potato tuber storage protein gene. Plant J. 5(6): 815—826. Gu, W. and Roeder, R. G. (1997) Activation of p53 sequence-specific DNA binding by acetylation of the p53 C-terminal domain. Cell 90(4): 595—606. Haigis, M. C. and Sinclair, D. A. (2010) Mammalian sirtuins: biological insights and disease relevance. Annu. Rev. Pathol. 5: 253—295. Hanna-Rose, W. and Hansen, U. (1996) Active repression mechanisms of eukaryotic transcription repressors. Trends Genet. 12: 229—234. Hao, Y., Wang, H., Qiao, S., Leng, L. and Wang, X. (2016) Histone deacetylase HDA6 enhances brassinosteroid signaling by inhibiting the BIN2 kinase. Proc. Natl. Acad. Sci. 113(37): 10418—10423. He,Y., Michaels, S. D. and Amasino, R. M. (2003) Regulation of flowering time by histone acetylation in Arabidopsis. Science 302: 1751—1754. Jiang, J., Ma, S., Ye, N., Jiang, M., Cao, J. and Zhang, J. (2017) WRKY transcription factors in plant responses to stresses. J. Integr. Plant Biol. 59(2): 86—101. Jiang, Y., Zeng, B., Zhao, H., Zhang, M., Xie, S. and Lai, J. (2012) Genome-wide transcription factor gene prediction and their expressional tissue-specificities in maize. J. Integr. Plant Biol. 54(9): 616—630. Kalde, M., Barth, M., Somssich, I. E. and Lippok, B. (2003) Members of the Arabidopsis WRKY group III transcription factors are part of different plant defense signaling pathways. Mol. Plant Microbe Interact 16(4): 295—305. Kiernan, R., Bres, V., Ng, R. W., Coudart, M. P., El Messaoudi, S., Sardet C., Jin, D. Y., Emiliani, S. and Benkirane, M. (2003) Post-activation turn-off of NF-κB-dependent transcription is regulated by acetylation of p65. J. Biol. Chem. 278:2758—2766. Kim, K. C., Lai, Z., Fan, B. and Chen, Z. (2008) Arabidopsis WRKY38 and WRKY62 transcription factors interact with Histone Deacetylase 19 in basal defense. Plant Cell 20: 2357—2371. Li, W., Wang, H. and Yu, D. (2016) Arabidopsis WRKY transcription factors WRKY12 and WRKY13 oppositely regulate flowering under short-day condition. Mol. Plant 9: 1492—1503. Liu, X., Wei, W., Zhu, W., Su, L., Xiong, Z., Zhou, M., Zheng, Y. and Zhou, D. X. (2017) Histone deacetylase AtSRT1 regulates metabolic flux and stress response in Arabidopsis. Mol. Plant doi: 10.1016/j.molp.2017.10.010. Llorca, C. M., Potschin, M. and Zentgraf, U. (2014) bZIPs and WRKYs: two large transcription factor families executing two different functional strategies. Front. Plant Sci. 5: 169. Luo, X., Sun, X., Liu, B., Zhu, D., Bai, X., Cai, H., Ji, W., Cao, L., Wu, J., Wang, M., Ding, X. and Zhu, Y. (2013) Ectopic expression of a WRKY homolog from Glycine soja alters flowering time in Arabidopsis. PLoS One 8(8): e73295. Maeo, K., Hayashi, S., Kojima-Suzuki, H., Morikami, A. and Nakamura K. (2001) Role of conserved residues of WRKY domain in the DNA-binding of tobacco WRKY family proteins. Biosci. Biotechnol. Biochem. 65(11): 2428—2436. Mangelsen, E., Kilian, J., Berendzen, K. W., Kolukisaoglu, U. H., Harter, K., Jansson, C. and Wanke, D. (2008) Phylogenetic and comparative gene expression analysis of barley (Hordeum vulgare) WRKY transcription factor family reveals putatively retained functions between monocots and dicots. BMC Genom. 9:194. Marı´n, I. C., Loef, I., Bartetzko, L., Searle, I., Coupland, G., Stitt, M. and Osuna, D. (2011) Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways. Planta 233, 539—552. Meek, D. W. and Anderson, C. W. (2009) Posttranslational modification of p53: cooperative integrators of function. Cold Spring Harb. Perspect. Biol. 1: a000950. Murfett, J., Wang, X. J., Hagen, G. and Guilfoyle, T. J. (2001) Identification of Arabidopsis histone deacetylase HDA6 mutants that affect transgene expression. Plant Cell 13(5): 1047—1061. Ohta, M., Ohme-Takagi, M. and Shinshi, H. (2000) Three ethylene-responsive transcription factors in tobacco with distinct transactivation functions. Plant J. 22(1): 29—38. Park, J. M., Jo, S. H., Kim, M. Y., Kim, T. H. and Ahn, Y. H. (2015) Role of transcription factor acetylation in the regulation of metabolic homeostasis. Protein Cell 6(11): 804—813. Polevoda, B. and Sherman, F. (2000) N-alpha-terminal acetylation of eukaryotic proteins. J. Biol. Chem. 275: 36479—36482. Ren, X., Chen, Z., Liu, Y., Zhang, H., Zang, M., Liu, Q., Hong, X., Zhu, J. K. and Gong, Z. (2010) ABO3, a WRKY transcription factor, mediates plant responses to abscisic acid and drought tolerance in Arabidopsis. Plant J. 63: 417—429. Rushton, P. J., Macdonald, H., Huttly, A. K., Lazarus, C. M. and Hooley, R. (1995) Members of a new family of DNA-binding proteins bind to a conserved cis-element in the promoters of α-Amy2 genes. Plant Mol. Biol. 29: 691—702. Sarris, P. F., Duxbury, Z., Huh, S. U., Ma, Y., Segonzac, C., Sklenar, J., Derbyshire, P., Cevik, V., Rallapalli, G., Saucet, S. B., Wirthmueller, L., Menke, F. L. H., Sohn, K. H. and Jones, J. D. G. (2015) A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell 161: 1089—1100. Shih, W. T. (2015) Functional analysis of Arabidopsis WRKY63 in salt stress response and flowering time control. (Master Thesis, National Taiwan University, Institute of Plant Biology) Retrieved from http://ntur.lib.ntu.edu.tw/handle/246246/ 274963#.WgRV81Vsa00. Sun, C., Palmqvist, S., Olsson, H., Borén, M., Ahlandsberg, S. and Jansson, C. (2003) A novel WRKY transcription factor, SUSIBA2, participates in sugar signaling in barley by binding to the sugar-responsive elements of the iso1 promoter. Plant Cell 15(9): 2076—2092. To, T. K., Nakaminami, K., Kim, J. M., Morosawa, T., Ishida, J., Tanaka, M., Yokoyama, S., Shinozaki, K. and Seki, M. (2011) Arabidopsis HDA6 is required for freezing tolerance. Biochem. Biophys. Res. Commun. 406(3): 414—419. Triezenberg, S. J. (1995) Structure and function of transcriptional activation domains. Curr. Opin. Genet. Dev. 5: 190—196. van Aken, O., Zhang, B., Law, S., Narsai, R. and Whelan, J. (2013) AtWRKY40 and AtWRKY63 modulate the expression of stress-responsive nuclear genes encoding mitochondrial and chloroplast proteins. Plant Physiol. 162: 254—271. van Verk, M. C., Pappaioannou, D., Neeleman, L., Bol, J. F. and Linthorst, H. J. (2008) A novel WRKY transcription factor is required for induction of PR-1a gene expression by salicylic acid and bacterial elicitors. Plant Physiol. 146(4): 1983—1995. Verdin, E. and Ott, M. (2015) 50 years of protein acetylation: from gene regulation to epigenetics, metabolism and beyond. Nat. Rev. Mol. Cell Biol. 16(4): 258—264. Wu, K., Zhang, L., Zhou, C., Yu, C.W. and Chaikam, V. (2008) HDA6 is required for jasmonate response, senescence and flowering in Arabidopsis. J. Exp. Bot. 59(2): 225—234. Yoo, S. K., Chung, K. S., Kim, J., Lee, J. H., Hong, S. M., Yoo, S. J., Yoo, S. Y., Lee, J. S. and Ahn, J. H. (2005) CONSTANS activates SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 through FLOWERING LOCUS T to promote flowering in Arabidopsis. Plant Physiol. 139(2): 770—778. Yu, C. W., Liu, X., Luo, M., Chen, C., Lin, X., Tian, G., Lu, Q., Cui, Y., and Wu, K. (2011) HISTONE DEACETYLASE6 interacts with FLOWERING LOCUS D and regulates flowering in Arabidopsis. Plant Physiol. 156: 173—184. Yu, Y., Hu, R., Wang, H., Cao, Y., He, G., Fu, C. and Zhou, G. (2013) MlWRKY12, a novel Miscanthus transcription factor, participates in pith secondary cell wall formation and promotes flowering. Plant Sci. 212:1—9. Yu, Y., Liu, Z., Wang, L., Kim, S. G., Seo, P. J., Qiao, M., Wang, N., Li, S., Cao, X., Park, C. M. and Xiang, F. (2016) WRKY71 accelerates flowering via the direct activation of FLOWERING LOCUS T and LEAFY in Arabidopsis thaliana. Plant J. 85: 96—106. Zhang, H., Zhao, Y. and Zhou, D. X. (2017) Rice NAD+-dependent histone deacetylase OsSRT1 represses glycolysis and regulates the moonlighting function of GADPH as a transcriptional activator of glycolytic genes. Nucleic Acids Res. doi: 10.1093/nar/gkx825. Zhang, L., Chen, L. and Yu, D. (2017) Transcription factor WRKY75 interacts with DELLA proteins to affect flowering. Plant Physiol. doi:10.1104/pp.17.00657. Zhao, Y., Wang, Y. and Zhu, W. G. (2011) Applications of post-translational modifications of FoxO family proteins in biological functions. J. Mol. Biol. 3: 276—282. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68056 | - |
| dc.description.abstract | WRKY轉錄因子為植物特有之一群蛋白質家族，其特色在於具有至少一個保守的WRKY結構域，WRKY轉錄因子可結合至目標基因啟動子之W-box上，進一步調控目標基因的表現。目前研究發現WRKY轉錄因子參與在生物逆境、非生物逆境以及植物生長發育之調控。本文主要研究阿拉伯芥轉錄因子WRKY63在開花時間之調控機制，以及其與組蛋白去乙醯基酶HDA6互作之生理意義。
我們實驗室利用酵母菌雙雜交篩選發現WRKY63可以與HDA6進行交互作用，並利用雙分子螢光互補實驗以及共免疫沉澱驗證了此交互作用。我們的研究發現WRKY63可能藉由促進FLC (FLOWERING LOCUS C) 開花抑制子之表現，進而影響開花。WRKY63突變株abo3無論是在長日照亦或是短日照條件下皆比野生型Col-0早開花；而HDA6突變株axe1-5則皆為晚開花；然而abo3 axe1-5雙突變體植株則呈現中間型之表現型。我們發現在abo3中，FLC之mRNA表現量比野生型Col-0低。此外，WRKY63在體外或體內皆可被乙醯基化修飾，而此修飾與否會進一步影響WRKY63之轉錄活性。利用在原生質體進行短暫表現檢測轉錄活性之方式，我們發現HDA6可以抑制WRKY63之轉錄活性。這些實驗結果指出WRKY63與HDA6之交互作用可能在開花時間之調控上扮演著相當重要的角色。 | zh_TW |
| dc.description.abstract | WRKY transcription factors are one plant-specific class of proteins containing at least one conserved WRKY domain. WRKYs regulate the expression of genes involved in plant responses to biotic/abiotic stresses and plant development by targeting to the W-box directly. Previously, it was found that histone deacetylase HDA6 can interact with WRKY63 in yeast two-hybrid assays. The interaction of HDA6 and WRKY63 was further confirmed by bimolecular fluorescence complementation assays and co-immunoprecipitation assays. In this study, we found that WRKY63 regulates flowering by regulating the expression of FLOWERING LOCUS C (FLC). The wrky63 loss-of-function mutant, abo3, showed early flowering under both long day (LD) and short day (SD) conditions. In addition, the transcript level of FLC was down-regulated in abo3 compared to Col-0 wild type. In contrast, the hda6 mutant, axe1-5, showed late flowering under both LD and SD. The abo3 axe1-5 double mutant displayed an intermediate flowering phenotype compared to the single mutants. Furthermore, WRKY63 can be acetylated both in vitro and in vivo, and this post-translational modification can influence its transcriptional activity. Using transient transcriptional activity assays in Arabidopsis protoplasts, we found that HDA6 inhibits the gene activation ability of WRKY63. Together, these results suggest that the interaction of WRKY63 and HDA6 play a critical role in controlling flowering time in Arabidopsis. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T02:11:54Z (GMT). No. of bitstreams: 1 ntu-106-R04b42022-1.pdf: 14342202 bytes, checksum: a2120e80f24f3b50224a54e48f15dacd (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 誌謝 i
摘要 ii ABSTRACT iii INDEX iv LIST OF FIGURES vi LIST OF SUPPLEMENTARY FIGURES vii LIST OF SUPPLEMENTARY TABLES viii LIST OF ABBREVIATIONS ix Chapter 1 Introduction 1 1.1 WRKY transcription factors 1 1.2 Functions of WRKY transcription factors in flowering 2 1.3 Arabidopsis WRKY63 participates in abiotic stress response and flowering time control..................................................................................................................... 4 1.4 Arabidopsis histone deacetylase HDA6 controls flowering time 5 1.5 Biological roles of transcription factor acetylation 5 1.6 The specific aims of this study 7 Chapter 2 Materials and Methods 9 2.1 Plant materials 9 2.2 Quick DNA extraction 9 2.3 RNA isolation 10 2.4 DNase treatment 11 2.5 Quantitative RT-PCR analysis 11 2.6 Chromatin immunoprecipitation (ChIP) assay 12 2.7 Bimolecular fluorescence complementation (BiFC) assay 18 2.8 Transient transcriptional activity assay 20 2.9 Purification of recombinant proteins, GST-HAG1 and GST-HAG2 22 2.10 Acetylation of non-histone proteins 23 2.11 LC-MS/MS analysis 24 Chapter 3 Results 27 3.1 Identification of homozygous T-DNA insertion mutants of WRKY63 27 3.2 Flowering phenotypes of wrky63 mutants and WRKY63 overexpression plants 27 3.3 Expression of flowering-related genes in abo3 28 3.4 Flowering phenotypes of abo3, ft-10, flc-3, axe1-5 and their double mutants 28 3.5 WRKY63 interacts with the histone acetyltransferase HAG1 29 3.6 WRKY63 can be acetylated 30 3.7 HDA6 represses the transcriptional activation ability of WRKY63 30 Chapter 4 Discussion 33 4.1 WRKY63 is involved in flowering time control 33 4.2 The activity of WRKY63 is regulated by acetylation 34 Figures 36 Supplementary Figures 53 Appendixes 59 Supplementary Tables 76 References 79 | |
| dc.language.iso | en | |
| dc.subject | 阿拉伯芥 | zh_TW |
| dc.subject | 組蛋白去乙醯基? | zh_TW |
| dc.subject | 開花時間 | zh_TW |
| dc.subject | WRKY63轉錄因子 | zh_TW |
| dc.subject | 轉譯後修飾 | zh_TW |
| dc.subject | FLC | en |
| dc.subject | WRKY63 | en |
| dc.subject | HDA6 | en |
| dc.subject | flowering time | en |
| dc.subject | Arabidopsis | en |
| dc.subject | post-translational modification | en |
| dc.subject | reproductive growth | en |
| dc.title | 阿拉伯芥轉錄因子WRKY63與組蛋白去乙醯基酶HDA6相互作用並調控開花時間 | zh_TW |
| dc.title | WRKY63 interacts with the histone deacetylase HDA6 and regulates flowering time in Arabidopsis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 106-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 吳素幸(Shu-Hsing Wu),謝旭亮(Hsu-Liang Hsieh),張英?(Ing-Feng Chang),王雅筠(Ya-Yun Wang) | |
| dc.subject.keyword | 阿拉伯芥,WRKY63轉錄因子,組蛋白去乙醯基?,開花時間,轉譯後修飾, | zh_TW |
| dc.subject.keyword | Arabidopsis,WRKY63,HDA6,FLC,flowering time,reproductive growth,post-translational modification, | en |
| dc.relation.page | 84 | |
| dc.identifier.doi | 10.6342/NTU201800006 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2018-01-05 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 植物科學研究所 | zh_TW |
| 顯示於系所單位： | 植物科學研究所 | |
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