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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 王安邦(Prof.An-Bang Wang) | |
dc.contributor.author | Subhashree Shivani | en |
dc.contributor.author | 施凡妮 | zh_TW |
dc.date.accessioned | 2021-06-17T02:11:33Z | - |
dc.date.available | 2020-01-11 | |
dc.date.copyright | 2018-03-01 | |
dc.date.issued | 2018 | |
dc.date.submitted | 2018-01-12 | |
dc.identifier.citation | 1. Lauffenburger DA et al., Cell migration: a physically integrated molecular process Cell 1996;84:359–69.
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Faia-Torres AB, et al., Differential regulation of osteogenic differentiation of stem cells on surface roughness gradients, Biomaterials. 2014 Nov;35(33):9023-32 16. Pham JT, et al., Guiding cell migration with microscale stiffness patterns and undulated surfaces, Acta Biomater. 2016 Jul 1;38:106-15 17. Qing Zhang, et al., Microgrooved polymer substrates promote collective cell migration to accelerate fracture healing in an in vitro model, Applied Materials and Interfaces, 2015 Oct 21;7(41):23336-45 18. K Anselme, et al., Relative influence of surface topography and surface chemistry on cell response to bone implant materials. Part 2: biological aspects, Proc Inst Mech Eng H. 2010 Dec;224(12):1487-507. 19. Wozniak, M. A., et al., Focal adhesion regulation of cell behavior, Biochimica et Biophysica Acta, 2004, 1692, 103–119. 20. Ingber, D. E., Cellular mechanotransduction: putting all the pieces together again, FASEB J., 2006, 20, 811–827. 21. Trepat, et al., Universality in cell mechanics, Soft Matter 2008, 4, 1750–1759. 22. Fujita, S., et al., Time-lapse observation of cell alignment on nanogrooved patterns , J. R. Soc. Interface, 2009, 6, S269–S277 23. M. Payer, et al., Cell growth on different zirconia and titanium surface textures: a morphologic in vitro study, Übersetzung: LinguaDent, DOI 10.3238/ZZI.2010.0338 24. G. Zhao, et al., Requirement for Both Micron and Submicron Scale Structure for Synergistic Responses of Osteoblasts to Substrate Surface Energy and Topography, Biomaterials. 2007 June ; 28(18): 2821–2829. 25. Yang, et al., Surface topography of hydroxyapatite promotes osteogenic differentiation of human bone marrow mesenchymal stem cells”, Mater. Sci. Eng., C 2016, 60, 45−53 26. Chunya Wu, et al., Effect of surface roughness on the initial response of MC3T3-E1 cells cultured on polished on titanium alloy, Biomedical Materials and Engineering 26 (2015) S155-S164 27. 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Gross, et al., Mineral heterogeneity has a minor influence on the apparent elastic properties of human cancellous bone: a SRuCT-based finite element study, ComputerMethods in Biomechanics and Biomedical Engineering, vol. 15, no. 11, pp. 1137–1144, 2012. 34. X. J. Wang, et al., Elastic modulus and hardness of cortical and trabecular bovine bone measured by nano indentation, Transactions of Nonferrous Metals Society of China (English Edition), vol. 16, supplement 2, pp. s744–s748, 2006. 35. M. Cuppone, et al., The longitudinal Young’s modulus of cortical bone in the midshaft of human femur and its correlation with CT scanning data, Calcified Tissue International, vol. 74, no. 3, pp. 302–309, 2004. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/68034 | - |
dc.description.abstract | 細胞之遷移對於傷口快速復原為一個重要之影響參數。而不同的環境也影響細胞能動性。現今研究上已有關於表面粗糙度扮演著一個重要的拓樸結構,其可以提升細胞的行為,然而對於控制沒有移動方向性之細胞仍然相當艱鉅。先前已有關於細胞遷移研究,其材料使用鈦金屬(Titanium)亦者其他金屬,其基質之硬度不同於骨頭。此外,若使用噴砂處理製造粗糙度之方式會引致尖頂形狀,將導致非均一化之粗糙度。
本研究有一系列實驗來了解關於粗糙度高以及粗糙度低之表面對於細胞在其上之移動速度、延緩、移動方向之角度與速率。我們使用快速激光雷射技術:雙光子聚合。利用生物相容性之材料(Ormocomp)使用雙光子聚合方式以製造出其模具,可得其表面之粗糙度分別為0.4 微米 與1.3 微米。奈米印製方法(Nano imprinting method)是用來去創造最終聚丙烯之基底。最終利用人類癌症骨細胞(MG63)接種於基底去研究細胞。 在表面粗糙度較高之材料顯示細胞提升其移動性並且在表面粗糙度較高之材料上,欄桿在移動方向上扮演了一個重要的角色;然而對於表面粗糙度較低之材料並沒有很有效果。根據結果,新的表面粗糙梯度會去測試細胞移動之效果,有趣的是:在沒有欄杆之結構上細胞有移動從粗糙度高至低之趨勢性。而材料結構面上之粗糙度梯度對於細胞移動扮演一個很重要之因素,在移動速度上會下降而增加其移動方向之角度。然而在放入欄杆之結構能促使細胞雙向移動。另外,當欄杆被排列成垂直表面粗糙度之梯時,其會限制細胞移動至另一處之速度,顯示了其性質對於表面粗糙度有較高之控制性。 這些設計可以進一步去探索有關於創建有表面粗糙度梯度之支架於更快速復原尤其是對於骨折之病患。 | zh_TW |
dc.description.abstract | Cell migration is an important parameter for faster wound healing. Various environmental cues can affect cell motility. Research have shown that roughness is an important topological cue that can enhance cell behavior. However, controlling the unidirectional migration of the cells is still a challenge. Previous studies on cell migration used Titanium or other metals as their substrates having much different stiffness than bone. In addition, use of sandblasting method to create roughness introduced sharp peaks that could result in non-uniform roughness.
In this study, a series of experiments were done on different roughness to understand the effect of higher and lower roughness on cell parameters such as migration velocity, migration delay, angle of orientation and rate of change of orientation. Uniform roughness of 0.4 µm and 1.3 µm were fabricated using rapid laser fabrication technique, two-photon polymerization (TPP) on a biocompatible material, Ormocomp to create mold. Nano imprinting method was used to create final PP substrates. Human osteosarcoma bone cells, MG63, were seeded on the substrates to analyze the cell parameters. It was demonstrated that higher roughness enhanced migration activities of the cells. Also, rails played an important role in orienting the cells in higher roughness whereas were ineffective in lower roughness. Based on these results, novel roughness gradients were designed to examine the effect on cell migration. Interestingly, cells biased their migration from higher roughness to lower roughness for no rail substrates. Area of each roughness on the gradient was also vital as larger area decreased the migration velocity of the cells and increased orientation angle. However, introduction of rails facilitated bidirectional migration. Also, when aligned perpendicularly to the gradient, rails restricted the migration of cells to one region, suggesting higher dominance on cell motility than roughness. These designs can be further explored for creating roughness gradient scaffolds for faster healing of especially fractured bones. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T02:11:33Z (GMT). No. of bitstreams: 1 ntu-107-R04543079-1.pdf: 5677997 bytes, checksum: d5e5bde52bacf0dffa0327b97d106bcb (MD5) Previous issue date: 2018 | en |
dc.description.tableofcontents | Acknowledgement i
Abstract v Content vii List of Figures xi List of Tables xxi Chapter. 1 Introduction 1 Chapter. 2 Motivation and Hypothesis 4 2.1. Motivation 4 2.2. Hypothesis 6 Chapter 3. Literature Review 12 3.1. Cell-Surface Interaction 12 3.2. Surface Roughness 14 3.3. Topotaxis 24 3.4. Two Photon Polymerization (TPP) 27 3.4.1. Working Principle 28 3.4.2. Applications 30 Chapter. 4 Materials and Methods 33 4.1. Creating the Substrates 33 4.2. Coating of the substrate 36 4.3. Assembling Substrate 37 4.4. MG63 cell Thawing 37 4.5. Cell Passaging 38 4.6. Device Preparation for Culturing MG63 Cells 38 4.7. Continuous Cell Imaging Setup 38 4.7.1. Bioreactor 39 4.7.2. Changing the media 41 4.7.3. Experimental Setup for Continuous Imaging 42 4.8. Cell Fixing 43 4.9. Immunostaining 44 4.10. Statistical Analysis 45 4.10.1. Calculating the Rz of the substrates 45 4.10.2. Calculating the cell parameters 46 Chapter. 5 Results and Discussion 47 5.1. Notation for Experimental Conditions 47 5.2. Calculating Cell Parameters 49 5.2.1. Migration velocity 49 5.2.2. Initial Migration Delay 50 5.2.3. Angle of Orientation (ɵ) 51 5.3. Uniform Roughness 52 5.3.1. Rz Measurement 52 5.3.2. Cell Migration 56 5.3.3. Migration Velocity 61 5.3.4. Angle of Orientation (ɵ) 63 5.4. Roughness Gradient (Equal width of 40 µm) 66 5.4.1. Gxy-I40 66 5.4.2. Gx-I40 72 5.4.3. Gy-I40 78 5.5. Gx-I20 82 5.5.1. Rz Measurement 82 5.5.2. Cell Migration 85 5.5.3. Migration Velocity 87 5.5.4. Angle of Orientation (ɵ) 88 5.6. Gxy-I20 89 5.6.1. Rz Measurement 89 5.6.2. Cell Migration 92 5.6.3. Migration Velocity 93 5.6.4. Angle of Orientation (ɵ) 95 5.7. Comparison of Cell Behavior on Roughness Gradient Patterns 95 5.7.1. Migration Velocity 95 5.7.2. Initial Migration Delay 97 5.7.1. Angle of Orientation (ɵ) 98 Chapter. 6 Conclusions and Future Work 102 6.1. Conclusions 102 6.2. Future Work 103 References 104 | |
dc.language.iso | en | |
dc.title | MG-63細胞在雙光子聚合粗糙表面之活體外分析 | zh_TW |
dc.title | An in-vitro Analysis of MG-63 Cells on Roughened Surface Fabricated by Two Photon Polymerization | en |
dc.type | Thesis | |
dc.date.schoolyear | 106-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 許聿翔(Prof.Yu-Hsiang Hsu) | |
dc.contributor.oralexamcommittee | 鍾添東(Prof. Tien Tung Chung),侯 詠德(Prof. Yung Te Hou),周涵怡(Prof. Han-Yi Chou) | |
dc.subject.keyword | 雙光子聚合,MG63,粗糙度梯度,無方向性之移動, | zh_TW |
dc.subject.keyword | Two-photon polymerization,MG63,roughness gradient,unidirectional migration, | en |
dc.relation.page | 107 | |
dc.identifier.doi | 10.6342/NTU201800024 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2018-01-12 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 應用力學研究所 | zh_TW |
顯示於系所單位: | 應用力學研究所 |
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