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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張煥宗(Huan-Tsung Chang) | |
| dc.contributor.author | Yu-Ting Tseng | en |
| dc.contributor.author | 曾于庭 | zh_TW |
| dc.date.accessioned | 2021-06-17T01:42:10Z | - |
| dc.date.available | 2019-07-31 | |
| dc.date.copyright | 2017-07-31 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-07-28 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67650 | - |
| dc.description.abstract | 金奈米粒子(gold nanoparticles, Au NPs)具有獨特光學特性、高穩定性、表面積大與良好生物相容性，故其作為催化劑、生物細胞顯影劑及化學和生化感測器方面，擁有相當大的潛力。本論文主要利用不同方法合成之金奈米粒子，探討其不同尺寸及表面修飾後之化學與光學特性的變化，並應用於生物感測器。論文共分為五個章節:第一章回顧金奈米粒子與螢光金奈米量子點(gold nanodots, Au NDs)的發展、合成、光學特性及應用。第二章藉由調控表面修飾之硫醇分子種類、密度及總量來改變合成11-巰基十一酸金奈米量子點(11-MUA–Au NDs)的速度及調控其螢光強度。第三章，內容是將(11-巰基十一烷基)-N, N, N-三甲基溴化銨 [(11-Mercaptoundecyl)-N,N-trimethylammonium bromide, 11-MUTAB] 修飾的金奈米量子點 (11-MUTAB–Au NDs)經過超音波震盪法處理，簡單調控金奈米量子點的大小、表面氧化態、硫醇分子修飾密度及螢光強度。第四章探討在紫外光照射的協助下調控表面不同硫醇分子的密度與比例，來改變金奈米量子點的合成速率及放光波長和強度。第五章，則是利用兒茶素合成金奈米粒子，修飾上適合體(aptamer)金奈米粒子，此奈米複合物可辨認腫瘤組織上目標蛋白質，並利用質譜儀所得到之金原子簇訊號來製成質譜顯影圖，可準確、快速的用來標定癌細胞及癌組織。 | zh_TW |
| dc.description.abstract | Gold nanoparticles (Au NPs) have been widely employed as catalysts, biosensors and as bioimaging agents due to their unique optical properties, high stability, large surface area, and high biocompatibility. This thesis focuses on the preparation, characterization, and application of Au NPs with different sizes. It is divided into five chapters. We review the background of Au NPs and photoluminescent gold nanodots (Au NDs) including their synthetic routes, optical properties and applications in detail. Our findings reveal that the photoluminescence of Au NDs depends on surface ligand density, ligand chain length and electron donation capability of alkanethiols, and also on the core size of the particles in Chapter 2. We developed a simple route to control the size, oxidation state, surface ligand density and photoluminescence (PL) of these (11-mercaptoundecyl)-N,N,N-trimethylammonium bromide–capped gold nanodots (11-MUTAB–Au NDs) through ultrasonic treatment in Chapter 3. In Chapter 4, our results indicate that the PL and reaction rate of 11-MUA assembly on the 11-MUTAB–Au NDs (11-MUA/11-MUTABAu NDs) could be controlled by accelerating the assembly of surface thiol ligands via UV irradiation. We report a simple, rapid and high-throughput cancer diagnosis system using functional nanoparticles consisting of poly(catechin) capped-gold nanoparticles (Au@PC NPs) and nucleoli-binding aptamer (AS1411)-conjugated small gold NPs (AS1411–Au NPs) in Chapter 5. The AS1411–Au NPs/Au@PC NPs can be used as a targeting agent in laser desorption/ionization mass spectrometry (LDI-MS)-based tumor tissue/cancer cell imaging via aptamer-protein recognition. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T01:42:10Z (GMT). No. of bitstreams: 1 ntu-106-D02223203-1.pdf: 7916753 bytes, checksum: 892ef519587651f2c5314babee35eaa0 (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 中文摘要 I
Abstract II Contents III Figure Contents VII Chapter 1 Introduction 1 1.1. Nanomaterials 2 1.2. Gold nanoparticle (Au NPs) 3 1.2.1 Syntheses of Au NPs 3 1.2.2 Applications of Au NPs 4 1.3. Photoluminescent Au NDs 6 1.4.1 Synthesis of Au NDs 6 1.4.2 Optical properties of Au NDs 7 1.4.3 Applications of Au NDs 8 1.4. Matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) 11 1.4.1 Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) 12 1.4.2 Matrix-assisted laser desorption/ionization image (MALDI image) 13 1.5. Motive of research 13 1.6. References 15 Chapter 2 Photoluminescent Gold Nanodots: Role of the Accessing Ligands 25 2.1 Introduction 26 2.2 Experimental section 27 2.2.1 Chemicals 27 2.2.2 Synthesis and characteristics of Au NPs 28 2.2.3 Synthesis of photoluminescent 11-MUAAu NDs in the presence of salt 28 2.2.4 Synthesis of 11-MUA/11-MUNTAu NDs, 11-MUA/LAAu NDs, 11-MUAAu NDs, MUNTAu NDs, and LAAu NDs 29 2.2.5 Characterization of Au NDs 29 2.3 Results and discussion 31 2.3.1 Synthesis of Au NDs 31 2.3.2 Effect of salt 32 2.3.3 Effect of alkanethiol ligand 37 2.3.4 Effect of numbers of thiol 40 2.3.5 Accelerate reaction time 41 2.4 Conclusions 42 2.5 References 44 Chapter 3 Ultrasound-mediated Modulation of the Emission of Gold Nanodots 65 3.1 Introduction 66 3.2 Experimental section 69 3.2.1 Materials 69 3.2.2 Synthesis and characterizations of Au NPs 69 3.2.3 Synthesis of photoluminescent Au NDs and ultrasonication 70 3.2.4 Characterization of Photoluminescent Au NDs 70 3.3 Results and discussion 72 3.3.1 Photoluminescent 11-MUTAB–Au NDs 72 3.3.2 Ultrasonication of 11-MUTAB–Au NDs 75 3.3.3 Effect of alkanethiol ligand 79 3.3.4 Effect of ultrasonication power 81 3.4 Conclusions 82 3.5 References 84 Chapter 4 Photoassisted Photoluminescence Fine-Tuning of Gold Nanodots through Free Radical-Mediated Ligand-Assembly 111 4.1 Introduction 112 4.2 Experimental section 114 4.2.1 Materials 114 4.2.2 Synthesis of photoluminescent 11-MUTAB–Au NDs 114 4.2.3 UV-light irradiation mediated ligand assembly on Au NDs 115 4.2.4 Characterization of Photoluminescent Au NDs 115 4.3 Results and discussion 117 4.3.1 Photoluminescent 11-MUTAB–Au NDs 117 4.3.2 Photoassisted ligand assembly 118 4.3.3 NIR vs. visible PL emission 120 4.3.4 Photobleaching and radicals formation 122 4.3.5 Effect of concentration and chain length of thiol ligands 125 4.3.6 PL switchable Au NDs 126 4.4 Conclusions 127 4.5 References 129 Chapter 5 Satellite-like Gold Nanocomposites for Targeted Mass Spectrometry Imaging of Tumor Tissues 159 5.1 Introduction 160 5.2 Experimental 161 5.2.1 Materials. 162 5.2.2 Synthesis and characterization of core-shell gold@poly(catechin) nanoparticles. 162 5.2.3 Preparation of satellite-like AS1411–Au NPs/Au@PC NP nanocomposites. 164 5.2.4 Analysis of tumor cells by AS1411–Au NPs/Au@PC NP–LDI-MS. 165 5.2.5 Tissue imaging. 166 5.2.6 Cell culture and cytotoxicity assays. 166 5.2.7 Immunohistochemistry with horseradish peroxidase. 167 5.3 Results and discussion 168 5.3.1 Synthesis and characterization of Au@PC NPs. 168 5.3.2 Preparation and characterization of AS1411–Au NPs/Au@PC NP. 171 5.3.3 LDI-MS of AS1411–Au NPs/Au@PC NP. 172 5.3.4 Specific detection of tumor cells. 173 5.3.5 Tumor tissue imaging. 175 5.4 Conclusions 176 5.5 References 178 Conclusions and Prospects 209 Publications 211 | |
| dc.language.iso | en | |
| dc.subject | 螢光金奈米量子點 | zh_TW |
| dc.subject | 金奈米粒子 | zh_TW |
| dc.subject | 光學特性 | zh_TW |
| dc.subject | 生物感測器 | zh_TW |
| dc.subject | Gold Nanodots | en |
| dc.subject | Gold Nanoparticles | en |
| dc.subject | Optical Properties | en |
| dc.subject | Biosensor | en |
| dc.title | 功能性金奈米粒子之合成與應用 | zh_TW |
| dc.title | Functional Gold Nanoparticles: Synthesis and Applications | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 黃志清(Chih-Ching Huang),林淑宜(Shu-Yi Lin),黃郁棻(Yu-Fen Huang),蘇正寬(Cheng-Kuan Su) | |
| dc.subject.keyword | 金奈米粒子,螢光金奈米量子點,光學特性,生物感測器, | zh_TW |
| dc.subject.keyword | Gold Nanoparticles,Gold Nanodots,Optical Properties,Biosensor, | en |
| dc.relation.page | 213 | |
| dc.identifier.doi | 10.6342/NTU201701986 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-07-28 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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