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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蔡宜芳 | |
| dc.contributor.author | Jeng-Jung Wu | en |
| dc.contributor.author | 吳任容 | zh_TW |
| dc.date.accessioned | 2021-06-17T01:41:20Z | - |
| dc.date.available | 2022-08-10 | |
| dc.date.copyright | 2017-08-10 | |
| dc.date.issued | 2017 | |
| dc.date.submitted | 2017-07-27 | |
| dc.identifier.citation | Albrecht, V., Ritz, O., Linder, S., Harter, K., and Kudla, J. (2001). The NAF domain defines a novel protein-protein interaction module conserved in Ca2+-regulated kinases. Embo J 20, 1051-1063.
Castaings, L., Camargo, A., Pocholle, D., Gaudon, V., Texier, Y., Boutet-Mercey, S., Taconnat, L., Renou, J.P., Daniel-Vedele, F., Fernandez, E. (2009). The nodule inception-like protein 7 modulates nitrate sensing and metabolism in Arabidopsis. Plant Journal 57, 426-435. Castro Marin, I., Loef, I., Bartetzko, L., Searle, I., Coupland, G., Stitt, M., and Osuna, D. (2011). Nitrate regulates floral induction in Arabidopsis, acting independently of light, gibberellin and autonomous pathways. Planta 233, 539-552. Cerezo, M., Tillard, P., Filleur, S., Munos, S., Daniel-Vedele, F., and Gojon, A. (2001). Major alterations of the regulation of root NO3- uptake are associated with the mutation of Nrt2.1 and Nrt2.2 genes in arabidopsis. Plant Physiology 127, 262-271. Crawford, N.M. (1995). Nitrate: nutrient and signal for plant growth. Plant Cell 7, 859-868. Drerup, M.M., Schlucking, K., Hashimoto, K., Manishankar, P., Steinhorst, L., Kuchitsu, K., and Kudla, J. (2013). The Calcineurin B-Like Calcium Sensors CBL1 and CBL9 Together with Their Interacting Protein Kinase CIPK26 Regulate the Arabidopsis NADPH Oxidase RBOHF. Molecular Plant 6, 559-569. Gordon, S.P., Heisler, M.G., Reddy, G.V., Ohno, C., Das, P., and Meyerowitz, E.M. (2007). Pattern formation during de novo assembly of the Arabidopsis shoot meristem. Development 134, 3539-3548. Ho, C.H., Lin, S.H., Hu, H.C., and Tsay, Y.F. (2009). CHL1 functions as a nitrate sensor in plants. Cell 138, 1184-1194. Huang, N.C., Liu, K.H., Lo, H.J., and Tsay, Y.F. (1999). Cloning and functional characterization of an Arabidopsis nitrate transporter gene that encodes a constitutive component of low-affinity uptake. Plant Cell 11, 1381-1392. Kiba, T., Feria-Bourrellier, A.B., Lafouge, F., Lezhneva, L., Boutet-Mercey, S., Orsel, M., Brehaut, V., Miller, A., Daniel-Vedele, F., Sakakibara, H. (2012). The Arabidopsis Nitrate Transporter NRT2.4 Plays a Double Role in Roots and Shoots of Nitrogen-Straved Plants. Plant Cell 24, 245-258. Kim, K.N., Cheong, Y.H., Grant, J.J., Pandey, G.K., and Luan, S. (2003). CIPK3, a calcium sensor-associated protein kinase that regulates abscisic acid and cold signal transduction in Arabidopsis. Plant Cell 15, 411-423. Konishi, M., and Yanagisawa, S. (2013). Arabidopsis NIN-like transcription factors have a central role in nitrate signalling. Nature Communications 4. Li, W.B., Wang, Y., Okamoto, M., Crawford, N.M., Siddiqi, M.Y., and Glass, A.D.M. (2007). Dissection of the AtNRT2.1 : AtNRT2.2 inducible high-affinity nitrate transporter gene cluster. Plant Physiology 143, 425-433. Liu, K.H., Huang, C.Y., and Tsay, Y.F. (1999). CHL1 is a dual-affinity nitrate transporter of arabidopsis involved in multiple phases of nitrate uptake. Plant Cell 11, 865-874. Liu, K.H., Niu, Y.J., Konishi, M., Wu, Y., Du, H., Chung, H.S., Li, L., Boudsocq, M., McCormack, M., Maekawa, S. (2017). Discovery of nitrate-CPK-NLP signalling in central nutrient-growth networks. Nature 545, 311-+. Mao, J., Manik, S.M., Shi, S., Chao, J., Jin, Y., Wang, Q., and Liu, H. (2016). Mechanisms and Physiological Roles of the CBL-CIPK Networking System in Arabidopsis thaliana. Genes (Basel) 7. Marchive, C., Roudier, F., Castaings, L., Brehaut, V., Blondet, E., Colot, V., Meyer, C., and Krapp, A. (2013). Nuclear retention of the transcription factor NLP7 orchestrates the early response to nitrate in plants. Nat Commun 4, 1713. Michaels, S.D., and Amasino, R.M. (1999). FLOWERING LOCUS C encodes a novel MADS domain protein that acts as a repressor of flowering. Plant Cell 11, 949-956. Muller, R., Borghi, L., Kwiatkowska, D., Laufs, P., and Simon, R. (2006). Dynamic and compensatory responses of Arabidopsis shoot and floral meristems to CLV3 signaling. Plant Cell 18, 1188-1198. Pandey, G.K., Cheong, Y.H., Kim, K.N., Grant, J.J., Li, L., Hung, W., D'Angelo, C., Weinl, S., Kudla, J., and Luan, S. (2004). The calcium sensor calcineurin B-like 9 modulates abscisic acid sensitivity and biosynthesis in Arabidopsis. Plant Cell 16, 1912-1924. Pandey, G.K., Grant, J.J., Cheong, Y.H., Kim, B.G., Li le, G., and Luan, S. (2008). Calcineurin-B-like protein CBL9 interacts with target kinase CIPK3 in the regulation of ABA response in seed germination. Mol Plant 1, 238-248. Sanchez-Barrena, M.J., Martinez-Ripoll, M., Zhu, J.K., and Albert, A. (2005). The structure of the Arabidopsis thaliana SOS3: Molecular mechanism of sensing calcium for salt stress response. J Mol Biol 345, 1253-1264. Srikanth, A., and Schmid, M. (2011). Regulation of flowering time: all roads lead to Rome. Cell Mol Life Sci 68, 2013-2037. Walch-Liu, P., Filleur, S., Gan, Y., and Forde, B.G. (2005). Signaling mechanisms integrating root and shoot responses to changes in the nitrogen supply. Photosynth Res 83, 239-250. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67634 | - |
| dc.description.abstract | 硝酸鹽不僅是植物必需的氮源，同時也是植物重要的信號分子。硝酸鹽對植物從營養期到繁殖期的轉變扮演著重要的一環。但植物如何偵測硝酸鹽來調節開花還所知甚少。先前研究顯示，NRT1家族中第10到第11個跨膜區域的脯胺酸（在NRT1.1序列中為第492個）在NRT1家族中為高度保守，且對於硝酸鹽轉運能力而非硝酸鹽的感受能力是極重要的。NRT1.13是一個膜蛋白且表達在木質部旁的薄壁組織細胞，在NRT1家族內是一個不具有此高度保守性脯胺酸的例外。相較於全營養環境，生長在氮限制環境下，nrt1.13-1單突變株表現出更延遲開花的性狀。因此本實驗室推測，NRT1.13可能是一個植物內部的硝酸鹽感受器，且能透過偵測木質部中硝酸鹽含量，調控植物開花。本篇研究的目的是想了解NRT1.13調控開花的分子機制。前人研究顯示，主要抑制開花基因FLC的表現量在nrt1.13-1突變株中上昇。我透過研究nrt1.13-1、flc-3及nrt1.13-1 flc-3雙突變株的開花情形來瞭解NRT1.13與FLC的關聯性。nrt1.13-1在低硝酸鹽的情況下會延遲開花，但在nrt1.13-1 flc-3雙突變株中這個性狀消失了，推測NRT1.13可能透過FLC調控開花。為了進一步找其他的下游基因，我們用酵母菌雙雜合研究尋找。結果顯示，蛋白激酶CIPK8與NRT1.13有結合能力，且CIPK8的調控區域和激酶區域都與NRT1.13有結合能力。植株的表現型分析發現，nrt1.13-1和cipk8-1雙突變株的開花性狀與野生型相似而非與nrt1.13-1相似，因此推測CIPK8可能是NRT1.13調控開花機制中的下游傳訊分子之一。第三部分，我們還觀察到轉錄因子NLP1、2、7和9的PB1區域對NRT1.13有結合能力，但全長NLP卻沒有。因此在開花調控中，NLPs是否同樣參與在NRT1.13的下游傳導並調控開花，將是非常有趣的問題。最後，我們觀察到NRT1.13並沒有表現在頂端分生組織中，顯示NRT1.13是在蓮座葉中而非頂端分生組織調控開花時間。綜合所有結果，我們找到了可能是位於NRT1.13下游的傳導信號分子，它們參與在NRT1.13應對氮源限制環境時調控開花的機制內。 | zh_TW |
| dc.description.abstract | Nitrate is not only an essential nutrient but also a signaling molecule for plants. Efficient utilization of nitrate is important for the conversion from vegetative stage to reproductive stage. Little is known about how plants sense nitrate to regulate flowering. Proline residue (492 in CHL1) between 10th and 11th transmembrane domains is highly conserved in NRT1 family and important for nitrate transportation but not for the nitrate sensing. NRT1.13, expressed in the xylem parenchyma cell, was an exception in NRT1 family without the highly conserved proline residue. In low nitrate condition, nrt1.13-1 showed delayed flowering. Previous results in our group suggested that NRT1.13 might be an internal sensor to monitor nitrate content in xylem and then regulate flowering. The aim of this study is to understand how NRT1.13 regulate flowering. Previous study showed that FLC, repressor of flowering, is up-regulated in nrt1.13-1. In this study, phenotype analysis showed that the nitrate-dependent late flowering phenotype of nrt1.13-1 is lost in the double mutant of nrt1.13 and flc-3, suggesting that NRT1.13 might regulate the flowering time through FLC. In the search for additional downstream candidates, yeast two-hybrid study showed that protein kinase CIPK8 interacts with NRT1.13. Both regulatory domain and kinase domain of CIPK8 can interact with NRT1.13. Phenotype analysis showed that the disruption of CIPK8 lead to early flowering while double mutant of nrt1.13-1 and cipk8-1 behave like as wild type instead of nrt1.13-1, suggesting that CIPK8 is required for the late flowering phenotype of nrt1.13-1, and it may act in the downstream of NRT1.13 to regulate flowering. In the third part, we also observed that the PB1 domain of transcription factor NLP1, 2, 7 and 9, can interact with NRT1.13, but the full-length of NLP cannot. In the last, it was found that NRT1.13 is not expressed in the shoot apical meristem, suggesting that NRT1.13 regulates the flowering time in the leaves instead of shoot apical meristem. Taken together, through these studies, we have identified several potential signal components in the downstream of NRT1.13 to regulate flowering in an N-dependent manner. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T01:41:20Z (GMT). No. of bitstreams: 1 ntu-106-R04b43030-1.pdf: 1562915 bytes, checksum: 5571aeae09e11c06dfa73882f1f4757f (MD5) Previous issue date: 2017 | en |
| dc.description.tableofcontents | 目錄
口試委員會審定書…………………………………………………. I 致謝……………………………………………………………….. II 中文摘要…………………………………………………............... III 英文摘要…………………………………………………............... IV 第一章 前言……………………………………………………….. 1 第二章 實驗材料與方法…………………………………………..... 7 第三章 結果………………………………………………………. 14 第四章 討論………………………………………………………. 22 圖表………………………………………………………………. 26 參考文獻………………………………………………………….. 50 | |
| dc.language.iso | zh-TW | |
| dc.subject | 硝酸鹽 | zh_TW |
| dc.subject | CIPK8 | zh_TW |
| dc.subject | NRT1.13 | zh_TW |
| dc.subject | 開花 | zh_TW |
| dc.subject | Nitrate | en |
| dc.subject | NRT1.13 | en |
| dc.subject | CIPK8 | en |
| dc.subject | flowering | en |
| dc.title | 探討NRT1.13在開花調控中的下游機制 | zh_TW |
| dc.title | The downstream mechanisms of NRT1.13 in flowering regulation | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 105-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李秀敏,董桂書 | |
| dc.subject.keyword | 硝酸鹽,NRT1.13,CIPK8,開花, | zh_TW |
| dc.subject.keyword | Nitrate,NRT1.13,CIPK8,flowering, | en |
| dc.relation.page | 53 | |
| dc.identifier.doi | 10.6342/NTU201702083 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2017-07-28 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
| 顯示於系所單位： | 分子與細胞生物學研究所 | |
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