請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67524
標題: | 草藥萃取物UH3443抑制胰腺癌侵襲力之研究 Herbal extracts UH3443 suppress pancreatic ductal adenocarcinoma cell invasion |
作者: | Yu-chi Yu 尤鈺淇 |
指導教授: | 李明學(Ming-Shyue Lee) |
關鍵字: | 胰腺癌,中草藥,侵襲力, Pancreatic cancer,Herbal extracts,Invasion, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 胰臟癌是目前最惡性的癌症之一,且治療效果非常不佳,病患的五年存活率低於5%。胰臟癌如此高的死亡率歸咎於其高侵襲轉移力,且癌症初期並無太多症狀,容易被疏忽,當被確診時大多已經轉移至淋巴及肝臟等其他臟器。目前主要用來治療轉移性癌症的療法是化學療法,但病患接受化學治療時,容易有嚴重的副作用。因此發展能有效抑制胰臟癌細胞侵襲能力且具低毒性、低副作用的藥物,是相當迫切更有其必要性。在先前的研究中,我們發現NTU04能夠抑制肝癌細胞侵襲力。我們進一步在台灣找到與NTU04同屬的植物(MSL-UH),並利用酒精萃取,發現MSL-UH使用液相層析分離出的F3和F4具有明顯抑制胰臟癌細胞侵襲能力的效果。在我的研究中,我們將F3和F4進一步分離出F3-4、F3-5、F4-1、F4-2和F4-3有較好的抑制癌細胞侵襲能力的活性的子分層,並將這些子分層收集命名為UH3443,發現UH3443在抑制胰腺癌細胞的侵襲力上有顯著的效果。此外,我們純化出UH3443中最主要的三個化合物(peak 1, 2 and 4),並且使用質譜儀與核磁共振儀測定解其分子結構。雖然這三個化合物在單獨的狀況下無法有效抑制胰臟癌細胞的侵襲能力,但是我們發現這三個主要化合物的組合能夠顯著的抑制PANC-1細胞約一半的侵襲力。綜合以上結果我們發現UH3443中所分離出來的三種化合物組合在抑制胰腺癌侵襲上相當具有潛力的。 Pancreatic cancer (PCa) is one of the most malignant cancers and has a low 5-year survival rate of 4-6%. The PCa high mortality is attributed to its highly invasive and metastatic capability. Chemotherapy is commonly used for the treatment of metastatic PCa; however, this treatment often causes patients severe side effects. Thus, developing a new medication to suppress PCa cell invasion, metastasis and progression with low toxicity is critically needed. In our previous study, we found herbal extract NTU04 could suppress the invasion ability of hepatic cancer cells, and then prepared the herbal extract (MSL-UH) from a local herb in Taiwan, which belongs to the same genus to NTU04. Moreover, we found that the fractions F3 and F4 of MSL-UH were effective to suppress PCa cell invasion. In this study, I further identify the more effective subfractions from F3 and F4, and found that the subfractions (F3-4, F3-5, F4-1, F4-2 and F4-3, named as UH3443) had a better suppressive activity than F3 and F4. Moreover, I purified the compounds in the four major peaks (1~4) in UH3443 and the compound structures in peak 1, 2 and 4 were identified. Although individual compounds in peak 1, 2 and 4 had no effect on the cell invasion, the combination effect of these 3 compounds could inhibit PCa cell invasion by approximately 50%. The results indicate that UH3443 and the three-compound mixture can serve as a potential herbal product to suppress PCa cell motility. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67524 |
DOI: | 10.6342/NTU201702314 |
全文授權: | 有償授權 |
顯示於系所單位: | 生物化學暨分子生物學科研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-106-1.pdf 目前未授權公開取用 | 3.47 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。