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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 李昆達(Kung-Ta Lee) | |
dc.contributor.author | Po-Ku Chen | en |
dc.contributor.author | 陳柏谷 | zh_TW |
dc.date.accessioned | 2021-06-17T01:21:15Z | - |
dc.date.available | 2022-08-14 | |
dc.date.copyright | 2017-08-14 | |
dc.date.issued | 2017 | |
dc.date.submitted | 2017-08-10 | |
dc.identifier.citation | Andres, E., N. H. Loukili, E. Noel, G. Kaltenbach, M. B. Abdelgheni, A. E. Perrin, M. Noblet-Dick, F. Maloisel, J. L. Schlienger, and J. F. Blickle. 2004. 'Vitamin B12 (cobalamin) deficiency in elderly patients', Can. Med. Assoc. Jour., 171: 251-9.
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Carmel, R. 2008. 'How I treat cobalamin (vitamin B12) deficiency', Blood, 112: 2214-21. Clarke, N. A., and P. B. Cowles. 1952. 'Studies on the host-virus relationship in a lysogenic strain of Bacillus megaterium. II. The relationship between growth and bacteriophage production in cultures of Bacillus megaterium', J Bacteriol, 63: 177-86. Coppen, A., and C. Bolander-Gouaille. 2005. 'Treatment of depression: time to consider folic acid and vitamin B12', J Psychopharmacol, 19: 59-65. Emerson, G. A. 1949. 'Growth promoting activity of vitamin B12 in rats receiving thyroid substance', Proc Soc Exp Biol Med, 70: 392-4. Eschenmoser, A. 1994. 'B12: reminiscences and afterthoughts', Ciba Found Symp, 180: 309-32. Fang, H., J. Kang, and D. Zhang. 2017. 'Microbial production of vitamin B12: a review and future perspectives', Microb Cell Fact, 16: 15. Fenech, M. 2012. 'Folate (vitamin B9) and vitamin B12 and their function in the maintenance of nuclear and mitochondrial genome integrity', Mutat Res, 733: 21-33. Ford, J. E. 1952. 'The microbiological assay of vitamin B12', Br J Nutr, 6: 324-30. Fowler, C. C., E. D. Brown, and Y. F. Li. 2010. 'Using a Riboswitch Sensor to Examine Coenzyme B-12 Metabolism and Transport in E. coli', Chem. Biol., 17: 756-65. Froese, D. S., G. Kochan, J. R. Muniz, X. Wu, C. Gileadi, E. Ugochukwu, E. Krysztofinska, R. A. Gravel, U. Oppermann, and W. W. Yue. 2010. 'Structures of the human GTPase MMAA and vitamin B12-dependent methylmalonyl-CoA mutase and insight into their complex formation', J Biol Chem, 285: 38204-13. Gruber, K., B. Puffer, and B. Krautler. 2011. 'Vitamin B12-derivatives-enzyme cofactors and ligands of proteins and nucleic acids', Chem Soc Rev, 40: 4346-63. Herbert, V., R. R. Streiff, and L. W. Sullivan. 1964. 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Rubenfield, S. Kieffer-Higgins, and G. M. Church. 1993. 'Characterization of the cobalamin (vitamin B12) biosynthetic genes of Salmonella typhimurium', J Bacteriol, 175: 3303-16. Ryan-Harshman, M., and W. Aldoori. 2008. 'Vitamin B12 and health', Can Fam Physician, 54: 536-41. Scalabrino, G. 2009. 'The multi-faceted basis of vitamin B12 (cobalamin) neurotrophism in adult central nervous system: Lessons learned from its deficiency', Prog Neurobiol, 88: 203-20. Schottker, B., M. A. Adamu, M. N. Weck, H. Muller, and H. Brenner. 2012. 'Helicobacter pylori infection, chronic atrophic gastritis and major cardiovascular events: a population-based cohort study', Atherosclerosis, 220: 569-74. Scott, A. I., and C. A. Roessner. 2002. 'Biosynthesis of cobalamin (vitamin B(12))', Biochem Soc Trans, 30: 613-20. Skeggs, H. R., H. M. Nepple, K. A. Valentik, J. W. Huff, and L. D. Wright. 1950. 'Observations on the use of Lactobacillus leichmannii 4797 in the microbiological assay of vitamin B12', J Biol Chem, 184: 211-21. Snell, E. E., and L. D. Wright. 1950. 'The watersoluble vitamins', Annu Rev Biochem, 19: 277-318. Taga, M. E., N. A. Larsen, A. R. Howard-Jones, C. T. Walsh, and G. C. Walker. 2007. 'BluB cannibalizes flavin to form the lower ligand of vitamin B12', Nature, 446: 449-53. Van Wyk, Jessy, and Trevor J. Britz. 2010. 'A rapid HPLC method for the extraction and quantification of vitamin B12 in dairy products and cultures of Propionibacterium freudenreichii', Dairy Sci Technol, 90: 509-20. Vary, P. S., R. Biedendieck, T. Fuerch, F. Meinhardt, M. Rohde, W. D. Deckwer, and D. Jahn. 2007. 'Bacillus megaterium--from simple soil bacterium to industrial protein production host', Appl Microbiol Biotechnol, 76: 957-67. Wang, P., Z. Zhang, Y. Jiao, S. Liu, and Y. Wang. 2015. 'Improved propionic acid and 5,6-dimethylbenzimidazole control strategy for vitamin B12 fermentation by Propionibacterium freudenreichii', J Biotechnol, 193: 123-9. Wangel, A. G., S. T. Callender, G. H. Spray, and R. Wright. 1968. 'A family study of pernicious anaemia. II. Intrinsic factor secretion, vitamin B12 absorption and genetic aspects of gastric autoimmunity', Br J Haematol, 14: 183-204. Warren, M. J., E. Raux, H. L. Schubert, and J. C. Escalante-Semerena. 2002. 'The biosynthesis of adenosylcobalamin (vitamin B-12)', Natural Product Reports, 19: 390-412. Wood, J. M. 2015. 'Bacterial responses to osmotic challenges', J Gen Physiol, 145: 381-8. Wu, W., S. Kang, and D. Zhang. 2013. 'Association of vitamin B6, vitamin B12 and methionine with risk of breast cancer: a dose-response meta-analysis', Br J Cancer, 109: 1926-44. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67144 | - |
dc.description.abstract | 本研究利用 Bacillus megaterium ATCC 10778來進行維生素B12 之生產,整個培養生產過程分為三階段,第一階段以好氧培養來累積菌體,第二階段則以厭氧培養來合成與累積維生素 B12 之前驅物,第三階段再以好氣培養將維生素 B12 前驅物合成為具生理活性之維生素 B12。本研究採用高效液相層析-紫外光 (HPLC-UV) 分析法及維生素B12 微生物分析法來進行 B. megaterium ATCC 10778 所生產出之維生素 B12 之定量。本研究利用搖瓶培養探討第二階段培養基組成 (三種前驅物之濃度:δ-aminolevulinic acid,簡稱ALA、5,6-dimethylbenzimidazole,簡稱DMB、鈷離子;葡萄糖濃度、氮源濃度及 pH) 及培養策略 (第二階段:厭氧培養期及第三階段:好氧培養期之培養時間) 對維生素 B12 產量之影響。發現含有 9% 葡萄糖濃度、7 % 氮源濃度及 pH 6.8 的 TB 調整培養基可顯著提高B. megaterium ATCC 10778 之維生素 B12 產量。此外在第二階段厭氧培養達30 小時及第三階段好氧培養達 14.5 小時亦可得較佳之維生素 B12 產量。根據上述最適之維生素 B12 生產條件,使用醱酵槽進行放大生產,在第二階段厭氧培養的部分,藉由添加氮氣來達到更佳之厭氧環境,使得最終維生素 B12 之產量達到 338.20 μg/L。 | zh_TW |
dc.description.abstract | In this study, I used Bacillus megaterium ATCC 10778 to produce vitamin B12 with a three-stage culture strategies. The first stage is to increase the number of bacteria, the second stage is for vitamin B12 precursor synthesis and accumulation under anaerobic condition, and the third stage is for completing the synthetic steps to transform precursors into bioactive vitamin B12 under aerobic condition. I used high performance liquid chromatography - ultraviolet detector method (HPLC-UV) and microbiological method to quantify vitamin B12 produced by B. megaterium ATCC 10778. I evalueted the effect of medium components on vitamin B12 production by suspension culture in Erlenmeyer flask. The concentrations of precursors (ALA, δ-aminolevulinic acid; DMB, 5,6-dimethylbenzimidazole; Co2+), glucose, nitrogen source, and pH value were all analyzed. Meanwhile, different duration of the second stage (anaerobic culture) and the third stage (aerobic culture) were also considered. I found the optimal condition for vitamin B12 production by B. megaterium ATCC 10778 is 9% (w/v) glucose and 7% (w/v) nitrogen source in the modified TB medium under pH 6.8. In addition, 30-hour anaerobic culture and 14.5-hour aerobic culture resulted in the highest vitamin B12 production. Based on the above-mentioned optimal conditions for vitamin B12 production, I scaled up the whole process in the fermenter with nitrogen gas addition to establish a better anaerobic environment in the second stage. The final production of vitamin B12 was up to 338.20 μg/L. | en |
dc.description.provenance | Made available in DSpace on 2021-06-17T01:21:15Z (GMT). No. of bitstreams: 1 ntu-106-R04b22015-1.pdf: 2119648 bytes, checksum: 37fe45cfd52c2c2ea7c70771e13cd3b7 (MD5) Previous issue date: 2017 | en |
dc.description.tableofcontents | 目錄
誌謝 i 摘要 ii ABSTRACT iii 縮寫表 iv 目錄 v 圖目錄 viii 壹、 前言 1 1.1 維生素B12 1 1.1.1 維生素B12簡介 1 1.1.2 維生素B12生理功能 2 1.1.3 維生素B12之生產 4 1.2 Bacillus megaterium 5 1.3 微生物法測量維生素 B12 含量的原理 7 1.4 研究目的 7 1.5 研究架構 8 貳、 材料與方法 9 2.1 菌株來源 9 2.2 B. megaterium 之培養 9 2.3 維生素B12之定量 10 2.3.1 B. megaterium 之維生素B12最佳萃取方法 10 2.3.2 微生物法定量維生素 B12 11 2.3.3 高效液相層析儀-紫外光法定量維生素 B12 12 2.4 統計方法 13 參、 結果與討論 14 3.1 B. megaterium 之維生素 B12 最佳萃取方法探討 14 3.2 探討第二階段培養基組成 15 3.2.1 前驅物濃度對 B. megaterium 生產維生素 B12 產量之影響 15 3.2.2 總體培養基濃度、葡萄糖濃度、氮源濃度對於 B. megaterium 生產維生素 B12 產量之影響 16 3.2.3 葡萄糖濃度對於 B. megaterium 生產維生素 B12 產量之影響 17 3.2.4 氮源濃度對於 B. megaterium 生產維生素 B12 產量之影響 17 3.2.5 pH 值對於 B. megaterium 生產維生素 B12 產量之影響 18 3.3 培養策略探討 18 3.3.1 第二階段 (厭氧培養期) 之培養時間對於 B. megaterium 生產維生素 B12 產量之影響 18 3.3.2 第三階段(好氧培養期)之培養時間對於 B. megaterium 生產維生素 B12 產量之影響 19 3.4 5 L 醱酵槽培養之探討 20 3.4.1 第一階段 (菌量提升期) 醱酵槽培養之探討 20 3.4.2 探討全階段醱酵槽培養之條件 21 肆、 結論 24 伍、 圖表 25 陸、 參考資料 36 柒、 附錄 42 圖目錄 圖 1、B. megaterium 之維生素 B12 最佳萃取方法探討 25 圖 2、前驅物濃度對 B. megaterium 生產維生素 B12 產量之影響 26 圖 3、總體培養基濃度、葡萄糖濃度、氮源濃度對於 B. megaterium 生產維生素 B12 產量之影響 27 圖 4、葡萄糖濃度對於 B. megaterium 生產維生素 B12 產量之影響 28 圖 5、氮源濃度對於 B. megaterium 生產維生素 B12 產量之影響 29 圖 6、pH 值 6.8 對於 B. megaterium生產維生素 B12 產量之影響 30 圖 7、pH 值對於 B. megaterium 生產維生素 B12 產量之影響 31 圖 8、第二階段 (厭氧培養期) 之培養時間對於 B. megaterium 生產維生素 B12 產量之影響 32 圖 9、第三階段 (好氧培養期) 之培養時間對於 B. megaterium 生產維生素 B12 產量之影響 33 圖 10、第一階段 (菌量提升期) 醱酵槽培養之探討 34 圖 11、探討全階段醱酵槽培養之條件 35 | |
dc.language.iso | zh-TW | |
dc.title | 以巨大芽孢桿菌生產維生素B12 | zh_TW |
dc.title | Production of vitamin B12 by Bacillus megaterium | en |
dc.type | Thesis | |
dc.date.schoolyear | 105-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 楊健志(Chien-Chih Yang),劉啟德(Chi-Te Liu) | |
dc.subject.keyword | 維生素B12,Bacillus megaterium,醱酵槽, | zh_TW |
dc.subject.keyword | Vitamin B12,Bacillus megaterium,fermenter, | en |
dc.relation.page | 46 | |
dc.identifier.doi | 10.6342/NTU201702779 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2017-08-10 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 生化科技學系 | zh_TW |
顯示於系所單位: | 生化科技學系 |
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