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標題: | 探討間質幹細胞對自體免疫疾病病人之T細胞的免疫調控能力及其機制 Study on the Immunoregulatory Effect of Mesenchymal Stem Cells on T Cells From Patients with Autoimmune Diseases and Its Mechanism |
作者: | Teng-Yuan Wei 韋登元 |
指導教授: | 莊雅惠 |
關鍵字: | 自體免疫疾病,間質幹細胞,T細胞,免疫調節,IL-33, autoimmune disease,mesenchymal stem cells (MSCs),T cells,immunomodulation,IL-33, |
出版年 : | 2017 |
學位: | 碩士 |
摘要: | 自體免疫疾病是一種慢性發炎的疾病,且第一型輔助性T細胞與第十七型輔助性T細胞在其致病機轉中扮演不可或缺的角色。間質幹細胞具有免疫抑制的功能,並且被廣泛地研究作為許多自體免疫疾病療法的可能性。在本篇論文中,我們研究分離自新來源的胎盤絨毛膜褪膜間質幹細胞(pcMSC)是否具有抑制自體免疫疾病病人之T細胞功能的能力。我們也進一步探討pcMSC免疫調控能力的機轉,並研究IL-33是否在其中扮演一定的角色。我們發現pcMSC無法抑制小兒自體免疫疾病病人之T細胞。重要的是,pcMSC可以顯著地抑制多發性硬化症與視神經脊髓炎病人之完全活化的T細胞,不過它也意外地對低度活化之T細胞表現免疫促進的效果。我們更進一步證明pcMSC的促進效果不須依靠細胞之間的接觸,且經IFN-前處理後亦無法避免此現象的發生。再者,pcMSC的抑制與促進能力皆與IL-33無關。總結來說,pcMSC具有治療多發性硬化症與視神經脊髓炎的潛能,但其免疫促進的效果必須被審慎評估。 Autoimmune disease is a type of chronic inflammatory disease, and T helper 1 (Th1) and T helper 17 (Th17) cells play a crucial role in its pathogenesis. Mesenchymal stem cells (MSC) possess immunosuppressive function and are widely studied as a potential cell therapy to many autoimmune diseases. Here, we investigated the therapeutic potential of the newly isolated placenta choriodecidual membrane-derived MSCs (pcMSCs) on T cell function of various autoimmune diseases. We also investigated the mechanisms of the immune-modulatory effects of pcMSCs, and evaluated whether IL-33 plays a role in them. We found that pcMSCs could not inhibit the T cells derived from pediatric autoimmune patients. Of note, pcMSCs significantly suppressed high-reactive T cells from adult multiple sclerosis (MS) and neuromyelitis optica (NMO) patients, although they displayed an unexpected enhancing effect on low-reactive T cells. We further demonstrated that the enhancing effect of pcMSCs is not dependent of cell-cell contact, and may not be prevented by IFN- pretreatment. Also, the suppressive and promoting function of pcMSCs were not mediated by IL-33. In conclusion, pcMSCs may have the potential to remedy MS and NMO, but their immune-enhancing effect should be carefully considered beforehand. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/67066 |
DOI: | 10.6342/NTU201702381 |
全文授權: | 有償授權 |
顯示於系所單位: | 醫學檢驗暨生物技術學系 |
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