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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 謝學真(Hsyue-Jen Hsieh) | |
| dc.contributor.author | Po-Hsun Chiu | en |
| dc.contributor.author | 邱柏勛 | zh_TW |
| dc.date.accessioned | 2021-06-17T01:09:59Z | - |
| dc.date.available | 2026-02-05 | |
| dc.date.copyright | 2021-03-22 | |
| dc.date.issued | 2021 | |
| dc.date.submitted | 2021-02-03 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66871 | - |
| dc.description.abstract | 本研究使用去乙醯度為91.7%之幾丁聚醣與縮水甘油基三甲基氯化銨(glycidyltrimethylammonium chloride, GTMAC)在純水中進行開環反應製備含有四級銨鹽官能基之水溶性幾丁聚醣(N-[(2-hydroxyl)-propyl-3-trimethylammonium] chitosan chloride, 簡稱HTCC)，並探討幾丁聚醣與GTMAC在不同反應條件與純化過程下之產率差異，再進一步使用FT-IR、NMR鑑定HTCC之分子結構；以導電度滴定法測定HTCC之取代度。實驗顯示反應體積161.857 ml、反應溫度85℃、GTMAC當量數為4，以及反應時間為6小時及8小時之HTCC產物，簡稱HTCC-6與HTCC-8具有較佳之產率、純度及胺基取代度。 隨後配製分別含有HTCC-6與HTCC-8之電紡溶液，測量溶液黏度等性質，再將電紡溶液在不同製程參數下製成電紡纖維，並以SEM觀察纖維型態，探討電紡參數與溶液性質對於纖維型態的影響，找出適合大量製備電紡纖維的操作條件，其中組成為C2.5H2.5G5P0.5AA20-6之電紡溶液在電壓25 kV、溶液流量0.05 ml/min之的電紡條件下之纖維型態最佳。 為提高纖維膜的穩定性，針對C2.5H2.5G5P0.5AA20-6纖維膜進行戊二醛蒸氣交聯，經探討發現交聯時間為1.5小時的纖維膜具有較佳的機械性質以及在水相環境中的穩定性。最後將交聯後的纖維膜進行抗菌性測試，結果發現纖維膜能在與大腸桿菌(菌株BL21(DE3))接觸的20小時內將細菌數減至極少，具有良好抗菌能力。 本研究成功合成水溶性幾丁聚醣衍生物HTCC，也成功製備了含有HTCC之複合電紡纖維膜，並且證實此纖維膜對於大腸桿菌具有良好的抗菌能力，未來可將此含有四級銨鹽化幾丁聚醣(HTCC)複合電紡纖維膜發展成為抗菌性生醫材料。 | zh_TW |
| dc.description.abstract | In this study, chitosan (degree of deacetylation: 91.7%) was used to react with glycidyltrimethylammonium chloride (GTMAC) to synthesize water-soluble chitosan — (N-[(2-hydroxyl)-propyl-3-trimethylammonium] chitosan chloride (HTCC), with quaternary salt functional groups. In addition, how the reaction yield and degree of substitution varied with reaction time and other reaction parameters were also investigated. The characteristics of HTCC were further examined by NMR, FT-IR and conductometric titration analysis. Eventually, two products (HTCC-6 and HTCC-8) had relatively better yields, purities and proper degree of substitutions. In order to find proper electrospinning parameters, various composite solutions containing HTCC-6 or HTCC-8 were prepared and used for fabrication of fibers by electrospinning device under different process parameters, such as applied voltage and solution flow rate. Based on the SEM images of these fibers, a solution named C2.5H2.5G5P0.5AA20-6 could be electrospun into fibers with desired morphologies under the conditions of applied voltage 25 kV and flow rate 0.05 ml/min. To increase the stability of fibers, the above-mentioned C2.5H2.5G5P0.5AA20-6 fibrous membrane was crosslinked by glutaraldehyde vapor. It was found that crosslinking time of 1.5 hours could enhance the tensile strength and stability of the fibrous membranes. The crosslinked membrane was subjected to in vitro antibacterial test. C2.5H2.5G5P0.5AA20-6 fibrous membrane was incubated with E. coli (strain BL21 (DE3)) for 20 hours and only very few bacteria survived on the fibrous membrane, indicating that C2.5H2.5G5P0.5AA20-6 fibrous membrane possessed great antibacterial activity against E. coli. at physiological pH (pH 7). In conclusion, the water-soluble chitosan derivative HTCC was successfully synthesized and used for fabrication of HTCC-containing composite electrospun fibrous membrane (C2.5H2.5G5P0.5AA20-6 membrane) which exhibited good antibacterial ability against E. coli, thus being a promising antibacterial biomaterial. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T01:09:59Z (GMT). No. of bitstreams: 1 U0001-0102202121041600.pdf: 7278840 bytes, checksum: 1ff176cafaa2c128a53a3c90cb7d6e85 (MD5) Previous issue date: 2021 | en |
| dc.description.tableofcontents | 致謝 I 摘要 III Abstract V 目錄 VII 圖目錄 XI 表目錄 XVII 縮寫與符號說明 XIX 中英名詞對照表 XXI 第一章 緒論 1 1.1 研究背景 1 1.2 研究流程與架構 3 第二章 文獻回顧 5 2.1 靜電紡絲法 5 2.1.1 靜電紡絲法發展及原理 5 2.1.2 靜電紡絲技術之影響因素 7 2.1.2.1 溶液性質 8 2.1.2.2 電紡製程參數 9 2.1.2.3 環境因素 11 2.1.3 靜電紡絲裝置分類 13 2.1.3.1 針頭設計方式 13 2.1.3.2 收集器裝置類型 17 2.1.4 靜電紡絲纖維在生物醫學的應用 18 2.2 生醫材料 19 2.2.1 幾丁聚醣 20 2.2.2 水溶性幾丁聚醣衍生物 22 2.2.3 明膠 24 2.2.4 聚氧化乙烯 26 2.3 交聯劑 27 第三章 實驗藥品、儀器與方法 31 3.1 實驗藥品 31 3.2 實驗儀器 33 3.3 實驗方法 35 3.3.1 HTCC合成與純化 35 3.3.1.1 Gt4C1V140-6、Gt4C1V140-8、Gt4C1V140-10合成與純化 35 3.3.1.2 Gt1C1V60-8、Gt2C1V60-8、Gt2C1V60-8、Gt4C1V60-10合成與純化 36 3.3.2 幾丁聚醣與HTCC之FT-IR圖譜測定 39 3.3.3 幾丁聚糖與HTCC之NMR圖譜測定 39 3.3.4 以導電度滴定法測定HTCC之胺基取代度 40 3.3.4.1 Fajans method標定硝酸銀溶液 40 3.3.4.2 硝酸銀標準溶液標定HTCC胺基取代度 41 3.3.5 電紡溶液配製 42 3.3.6 電紡溶液物化性質分析 44 3.3.6.1 導電度測定 44 3.3.6.2 黏度測定 44 3.3.6.3 表面張力測定 45 3.3.7 電紡纖維膜製備 46 3.3.7.1 合適大量製備之電紡參數尋找 46 3.3.7.2 電紡纖維膜大量製備 47 3.3.8 電紡纖維膜交聯 50 3.3.9 電紡纖維膜特性分析 50 3.3.9.1 掃描式電子顯微鏡(Scanning Electron Microscope)觀察型態 50 3.3.9.2 膜材機械性質分析 51 3.3.9.3 纖維直徑與孔洞直徑分析 52 3.3.9.4 崩解性測定 53 3.3.10 電紡纖維膜抗菌性測定 54 3.3.10.1 細菌培養及CFU/ml對O.D.值之檢量線製作 54 3.3.10.2 抗菌生長測試 55 第四章 結果與討論 57 4.1 HTCC合成與純化實驗結果 57 4.2 幾丁聚醣與HTCC之FT-IR圖譜鑑定結果 60 4.3 導電度滴定法測定HTCC之胺基取代度 64 4.4 幾丁聚醣與HTCC之NMR圖譜鑑定結果 68 4.5 靜電紡絲溶液物化性質分析 71 4.5.1 溶液黏度分析 71 4.5.2 溶液導電度分析 74 4.5.3 溶液表面張力分析 76 4.6 製程參數對奈米纖維型態之影響 78 4.6.1 不含明膠、幾丁聚醣、醋酸之電紡溶液纖維型態探討 78 4.6.1.1 HTCC-6與PEO濃度變化對纖維型態影響探討 78 4.6.1.2 HTCC-8與PEO濃度變化對纖維形態影響探討 85 4.6.2 含明膠、幾丁聚醣、醋酸之電紡溶液纖維形態探討 91 4.6.2.1 HTCC-8與幾丁聚醣濃度變化對纖維形態影響 91 4.6.2.2 HTCC-6與幾丁聚醣濃度變化對纖維形態影響探討 97 4.7 電紡纖維膜特性分析 101 4.7.1 電紡纖維膜直徑與孔洞大小分析 101 4.7.2 電紡纖維膜機械性質分析 103 4.7.3 電紡纖維膜崩解性測試 106 4.8 電紡纖維膜抗菌性測定 111 4.8.1 CFU值對O.D.值檢量線 111 4.8.2 體外抗菌生長實驗 112 第五章 結論與未來研究方向 115 5.1 結論 115 5.2 未來研究方向 118 參考文獻 121 | |
| dc.language.iso | zh-TW | |
| dc.title | 提升抗菌性之四級銨鹽化幾丁聚醣合成及幾丁聚醣複合電紡纖維膜之製備與特性探討 | zh_TW |
| dc.title | Synthesis of Quaternized Chitosan with Enhanced Antimicrobial Activity and Preparation and Characterization of Chitosan Composite Electrospun Fibrous Membranes | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 109-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 王大銘(Da-Ming Wang),謝子陽(Tzu-Yang Hsien) | |
| dc.subject.keyword | 幾丁聚醣,四級銨鹽化幾丁聚醣,靜電紡絲,抗菌材料, | zh_TW |
| dc.subject.keyword | chitosan,quaternized chitosan,electrospinning,antibacterial material, | en |
| dc.relation.page | 133 | |
| dc.identifier.doi | 10.6342/NTU202100347 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2021-02-04 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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