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標題: | 日長對紅龍果萌芽型態之影響 Effect of day-length on budding types in pitaya |
作者: | Bo-Ru Su 蘇柏如 |
指導教授: | 楊雯如(Wen-Ju Yang) |
關鍵字: | 花芽創始,花芽誘導,氯砒?,終止長日,部分創始型芽體, floral initiation,floral induction,forchlorfenuron,short-day interruption,partial initiated bud, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 紅龍果(Hylocereus spp.)為典型長日植物,臨界日長約為12小時,誘導季及非誘導季以春、秋分為界;成熟的當年生枝條在春分後即啟動花芽創始,分生組織中心形成花原體、外圍分化苞片。初次萌花為春分後8週後,此後於誘導季節間呈批次性萌花。枝條進入非誘導季後,芽體外圍苞片雖可持續分化而膨大,但需輔以暗中斷處理方可萌花。然而,已具花原體之未萌芽,氯砒脲(forchlorfenuron, CPPU)的施用可取代暗中斷處理,顯示長日誘導本身並非必須。因此,本研究首先定義未萌芽完成花原體的關鍵時間,即定義紅龍果芽體花芽形成的不歸點,再探討當年生成熟枝條花原體初次形成的時間,以及秋分後這些芽體CPPU催萌反應消退情形。
發育中的芽體可依其膨大程度分成4級,即0-3級芽;其中,3級芽已具有花原體及花瓣。本研究印證3級芽體在移除長日誘導環境後仍可繼續萌花,達到不歸點。當年生成熟枝條於長日誘導6週後,首出現3級芽,顯示由營養芽轉變為生殖芽至少需要誘導6週。芽體受長日誘導越久,CPPU的催萌效果越好,越能萌出具經濟生產能力的花苞。秋分後,三週內已達不歸點的芽體可以CPPU催萌,超過三週即無法再單憑CPPU催花。本研究同時也驗證紅龍果枝條in vivo 及 in vitro試驗的光週反應結果相近。 Pitaya (Hylocereus spp.) is a typical long-day plant with a critical daylength of 12 h, in that spring and autumn equinoxes play as the switches between inductive and non-inductive seasons. The matured current shoot of pitaya may onset floral initiation soon after spring equinox, forming floral primordia in the central zone and bracts in the peripheral zone of meristem. The first flush of flower emerges 8 weeks after spring equinox and continuously emerge several flushes of flower during inductive season. After autumn equinox, entering non-inductive season, the buds keep swelling because the bract differentiating is not ceased; however, night break treatment is required for budding. In the case of a not yet emerged bud with floral primordia, floral bud can be forced by forchlorfenuron (CPPU) instead of night breaking treatment, long daylength induction. Therefore, the aim of this study was first to find the critical stage of a not yet emerged bud and define the determined stage. Secondly, finding the timing that the determined bud first formed in the matured current shoots. Finally, exploring the declining of CPPU response activity of the determined buds after autumn equinox. The developing buds can be classified into 4 stages, stage 0-3, according to the swelling. In stage 3 buds, the floral primordia are formed and surrounded by some petals. In the present study, we confirm that stage 3 buds are able to emerge floral buds without long daylength environment, which indeed reach determination. In the matured current shoots, stage 3 buds appeared since 6 weeks of long daylength induction indicates that 6 weeks of induction was required for a vegetative primordia developed into a floral primordia. The longer the induction period, the stronger budding activity in response to CPPU and resulted in producing more economic valuable flower buds. After autumn equinox, CPPU response of the determined bud can retain 3 weeks and no response after then. In this study, we also demonstrated that the light induction experiment can be conducted in vitro or in vivo in pitaya. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/66371 |
DOI: | 10.6342/NTU202000224 |
全文授權: | 有償授權 |
顯示於系所單位: | 園藝暨景觀學系 |
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