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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 郭宗甫 | |
| dc.contributor.author | Yung-Ann Chen | en |
| dc.contributor.author | 陳韻安 | zh_TW |
| dc.date.accessioned | 2021-05-17T09:15:31Z | - |
| dc.date.available | 2012-08-15 | |
| dc.date.available | 2021-05-17T09:15:31Z | - |
| dc.date.copyright | 2012-08-15 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-09 | |
| dc.identifier.citation | 1. 中華民國痛風之友協會會刊第69期。2011。
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Purine-rich foods intake and recurrent gout attacks. Ann Rheum Dis. May 30, 2012. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6632 | - |
| dc.description.abstract | 人類在演化過程中失去可將尿酸轉化為尿素排出的尿酸酶，故尿酸合成過多或排泄減少是高尿酸血症主要形成的原因，而因營養過剩所造成的高尿酸血症與痛風更是現代人常見的文明病。主要位於肝臟的黃嘌呤酶是人體內嘌呤代謝過程產生尿酸的關鍵酵素，如果能抑制黃嘌呤酶作用便可以減低尿酸的形成，多餘的嘌呤可經由體內其他酵素回收利用，避免尿酸其在體內累積對關節、腎臟和心血管系統造成傷害。
近年來天然物萃物和自然保健食品已蔚為風潮，而植物化學成分中之抗氧化劑更是許多研究重心。龍眼除了是常見的熱帶水果更是中國傳統醫學中常見補神健胃的藥方，目前已有研究發現龍眼萃取物含有多種抗氧化成分，而抗氧化劑也被證實對許多酵素會產生影響。目前臨床常用之抗高尿酸血症藥物多為化學合成物質，有許多如：過敏、皮疹和肝腎傷害等不良副作用。由天然物中萃取能夠抗高尿酸血症又無副作用的植物化學成分是許多研究的目標。本實驗為證實民間偏方龍眼花茶之降尿酸療效，對其萃取物進行體外和體內之生物活性試驗，且為了充分利用生物資源，一併對龍眼種子、果殼、枝枒和樹葉進行研究。 實驗先以風乾龍眼﹙花、種子、果殼、枝枒和樹葉﹚甲醇萃取物在295nm波長下進行黃嘌呤酶抑制試驗測試龍眼萃取物抑制尿酸形成作用，發現五種龍眼萃取物都具有程度不等之黃嘌呤酶抑制效果，其中以龍眼花效果最佳，其IC50 為115.76 μg/ml。 另外測試龍眼花所含已知十種化合物之黃嘌呤酶抑制率，發現以proanthocyanidin A2效果最佳，其抑制率可達70.67%。之後進行生物體內活性試驗，在分別口服五種龍眼萃取物1小時後，以腹腔注射尿酸酶抑制劑oxonic acid，建立囓齒類動物﹙大鼠和小鼠﹚高尿酸血症模式，2小時後測量小鼠血中尿酸值的變化並和對照組比較，發現在100mg/kg劑量下五種龍眼甲醇萃取物都有降低小鼠血中尿酸值的效果﹙p< 0.01﹚。另外以高尿酸血症模式大鼠進行尿酸促進排除試驗，注射oxonic acid 2.5小時後，每30分鐘抽取膀胱尿液連續三小時。尿液分析結果顯示龍眼萃取物對促進尿酸排泄並無影響。綜合本研究之實驗結果，可以推測龍眼萃取物主要是藉由抑制黃嘌呤酶作用來降低血中尿酸值，並以龍眼花萃取物效果最佳，其作用主要是由具強抗氧化活性的proanthocyanidin A2對黃嘌呤酶產生抑制效果。 | zh_TW |
| dc.description.abstract | Hyperuricemia results from over production or/and underexcretion of uric acid. Uric acid production is catalyzed by xanthine oxidase (XO) in the liver, which is a key enzyme in the oxidizing process of xanthine into uric acid. This study investigated anti-hyperuricemia bioactivity of Dimocarpus longan Lour. Longan pulp is a Chinese medicinal plant used for the treatment of amnesia and its flower is folklorically made into drinking tea to lower serum uric acid level. Therefore, the methanol extracts of the flower, seed, shell, stem and leaf of Longan were tested in vitro and in vivo to confirm its anti-hyperuricemic effect. Firstly, xanthine oxidase suppression rates were evaluated by the xanthine oxidase inhibition assay. The results showed that all Longan extracts reduced blood uric acid level as compared to the control group (p < 0.01). The flower extract exibited the best blood uric acid lowering effect and IC50 of XO inhibition was 115.76 μg/ml. 10 compounds have been previously isolated from Longan flower in other studies; in this study, we also examined them by the xanthine oxidase inhibition assay, revealing that proanthocyanidin A2 had the strongest inhibitory effect (70.67%). In the in vivo part of this study, the methanol extracts from 5 different Longan parts were orally administrated to different groups of mice at 50 mg/kg or 100 mg/kg 1 hour before oxonic acid was injected. The blood uric acid levels in mice were then detected 2 h later. Furthermore, we carried out the urine uric acid excretion test in rats to find out whether the Longan extracts could promote the excretion of uric acid; however no significant effect on the excretion of uric acid was observed. According to these results, the mechanism behind the antihyperuricemic effect of Longan methanol extracts was indicated to be associated with the inhibition of xanthine oxidase which results in decreased production of uric acid. This study not only revealed the existence of antihyperuricemic effect of of Dimocarpus longan, but also explored possible mechanisms. Our results showed that Dimocarpus longan has a great potential to be developed into novel therapeutic agents for the treatment of hyperuricemia. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-17T09:15:31Z (GMT). No. of bitstreams: 1 ntu-101-R98629021-1.pdf: 972603 bytes, checksum: ea4a24a6de35092c296e72b7f557d2bd (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 目錄
致謝 i 摘要 ii Abstract iv 目錄 vi 圖目錄 viii 表目錄 ix 英文縮寫對照表 xi 第一章 序言 1 第二章 文獻回顧 3 第一節 龍眼介紹 3 2-1.1 龍眼學名、產地及特性 3 2-1.2 龍眼所含之植物化學成分 4 2-1.3龍眼之藥理與生物活性 5 第二節 尿酸與高尿酸血症 6 2-2.1 尿酸生合成 6 2-2.2黃嘌呤酶之特性 7 2-2.3 高尿酸血症 8 2-2.4 高尿酸血症與疾病 9 2-2.5 高尿酸血症與痛風流行病學調查 11 2-2.6 高尿酸血症與痛風的治療 13 2-2.7 高尿酸血症的預防 14 2-2.8 高尿酸血症動物模式 15 第三節 可以降尿酸之天然物 16 第三章 材料與方法 21 第一節 五種龍眼樣本製備 21 3-1.1龍眼萃取物之製備 21 3-1.2 龍眼花成分之取得 21 第二節 龍眼萃取物之生物活性篩選 22 3-2.1黃嘌呤酶抑制試驗 22 第三節 高尿酸血症動物模式建立 23 3-3.1 實驗動物 23 3-3.2實驗設計及操作 23 3-3.3 資料分析與統計 24 第四節 高尿酸血症模式小鼠研究龍眼降低血中尿酸值效力試驗 24 3-4.1 龍眼五種甲醇粗萃物降低血中尿酸值效力試驗 24 3-4.2 資料分析與統計 25 第五節 高尿酸血症模式大鼠研究龍眼萃取物促進尿酸排泄試驗 25 第四章 結果 26 第一節 生物活性篩選結果 26 第二節 高尿酸血症動物模式建立 26 第三節 高尿酸血症模式小鼠研究龍眼萃取物降低血中尿酸值效力試驗 27 4-3.1 龍眼五種甲醇粗萃物降低血中尿酸值效力結果 27 第四節 高尿酸血症模式大鼠研究龍眼萃取物促進尿酸排泄試驗結果 27 第五章 討論 29 參考文獻 35 英文版 66 圖目錄 圖一 龍眼之花和果實 46 圖二 已鑑定出之龍眼成分 46 圖三 尿酸合成的代謝路徑 47 圖四 人體內尿酸之平衡 47 圖五 具黃嘌呤酶抑制作用之植物化學成分 49 圖六 氧嗪酸鉀誘導小鼠產生高尿酸血症之持續時間 50 圖七 龍眼五種甲醇萃取物在100 mg/kg口服劑量下對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 50 圖八 龍眼五種甲醇萃取物在75 mg/kg口服劑量下對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 51 圖九 龍眼五種甲醇萃取物在50 mg/kg口服劑量下對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 52 圖十 三種龍眼甲醇萃取物在口服100 mg/kg劑量下對以氧嗪酸鉀誘導產生高尿酸血症大鼠尿酸排泄的影響 52 圖十一 原花青素A2之化學結構 53 表目錄 表一 不同年級之台灣國中生的血清尿酸平均值、標準差及過高盛行率 53 表二 不同居住地區之台灣國中生的血清尿酸平均值、標準差及過高盛行率 54 表三 龍眼五種萃取物對黃嘌呤酶抑制試驗之百分之五十抑制濃度 55 表四 龍眼五種甲醇粗萃物以有機溶劑分配層析各分層對黃嘌呤酶之抑制率 56 表五 龍眼所含的十種化合物之黃嘌呤酶抑制百分比 57 表六 以氧嗪酸鉀誘導小鼠產生高尿酸血症可維持的時間 58 表七 以PBS為對照組對小鼠血中尿酸值的影響 59 表八 龍眼五種甲醇萃取物在100mg/kg劑量下和allopurinol以及PBS對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 60 表九 龍眼五種甲醇萃取物在75 mg/kg劑量下和allopurinol以及PBS對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 61 表十 龍眼五種甲醇萃取物在50mg/kg劑量下和allopurinol以及PBS對以氧嗪酸鉀誘導產生高尿酸血症小鼠血中尿酸值的影響 62 表十一 龍眼花萃取物在100 mg/kg劑量下對以氧嗪酸鉀誘導產生高尿酸血症大鼠尿中尿酸值的影響 62 表十二 龍眼種子萃取物在100 mg/kg劑量下對以氧嗪酸鉀誘導產生高尿酸血症大鼠尿中尿酸值的影響 63 表十三 龍眼殼萃取物在100 mg/kg劑量下對以氧嗪酸鉀誘導產生高尿酸血症大鼠尿中尿酸值的影響 63 表十四 以PBS作為陰性對照組觀察其對以氧嗪酸鉀誘導產生高尿酸血症大鼠尿中尿酸值的影響 64 表十五 以PBS為對照組對未經氧嗪酸鉀誘導產生高尿酸血症之大鼠尿中尿酸值的影響 64 表十六 促進高尿酸血症之大鼠尿中尿酸排泄試驗中每組各時間點間尿中尿酸值斜率之比較 65 表十七 75%酒精分別萃取5g新鮮和烘乾龍眼殼，200倍稀釋後之黃嘌呤酶抑制率…… 65 | |
| dc.language.iso | zh-TW | |
| dc.subject | 黃嘌呤酶 | zh_TW |
| dc.subject | 降尿酸 | zh_TW |
| dc.subject | 龍眼 | zh_TW |
| dc.title | 龍眼花、殼、種子、葉與枝椏萃取物之降尿酸研究 | zh_TW |
| dc.title | The study of the extracts of flower, shell, seed, leaves and twig of Dimocarpus longan Lour. on reducing uric acid level. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.coadvisor | 徐鳳麟 | |
| dc.contributor.oralexamcommittee | 劉朝鑫,郭悅雄 | |
| dc.subject.keyword | 龍眼,降尿酸,黃嘌呤酶, | zh_TW |
| dc.subject.keyword | Dimocarpus longan,antihyperuricemia,xanthine oxidase inhibition, | en |
| dc.relation.page | 86 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2012-08-09 | |
| dc.contributor.author-college | 獸醫專業學院 | zh_TW |
| dc.contributor.author-dept | 獸醫學研究所 | zh_TW |
| 顯示於系所單位： | 獸醫學系 | |
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