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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 劉懷勝(Hwai-Shen Liu) | |
| dc.contributor.author | Yun-Chi Chen | en |
| dc.contributor.author | 陳勻錡 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:14:02Z | - |
| dc.date.available | 2017-07-20 | |
| dc.date.copyright | 2012-07-20 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-09 | |
| dc.identifier.citation | Anfinsen C.B., Anson M.L., Edsall J.T., Richards F.M., Advances in protein chemistry, Academic press, New York and London, 1968; 23; 122-127
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65866 | - |
| dc.description.abstract | 在蛋白質復性程序中，錯誤的摺疊與聚集體的形成是使復性效率不能提高的主要原因，因此於傳統的直接稀釋復性法中，皆利用大量的復性劑降低蛋白質之濃度並且稀釋變性劑之濃度，以利蛋白質之復性。但於本研究中，利用透析復性法，證實除了錯誤的摺疊與聚集體之外，復性溶液中之還原態二硫代蘇糖醇(dithiothreitol，DTTred)亦是影響蛋白質復性效率的重要因子。有鑑於此，本研究提出一於低稀釋倍率下之高效率復性策略：亦即於低稀釋倍率下，藉由添加氧化態榖胱甘肽(glutathione，GSSG)與DTTred反應，進而提升最終復性產率為傳統操作之13倍，並同時降低操作成本為傳統操作成本之40 %。
此外，本研究亦利用大小排阻層析法(size exclusion chromatography，SEC)可以於復性初期即將影響復性效果之DTTred分離的特性，對高濃度蛋白質進行復性。為了避免蛋白質因濃度過高而於管柱前端形成聚集體影響復性，本研究提出chaperon solvent plug復性方法，並提出最適當之chaperon solvent plug體積及樣品載入之時間，有效地抑制注射閥與管柱間之聚集體形成，避免影響復性效率。並透過對多段式流速復性法及尿素梯度復性法之了解後，提出一較佳之SEC復性策略：在高流速及chaperon solvent plug之保護下，將變性蛋白質載入至系統中，待其進入至SEC管柱後，則於管柱內提供尿素梯度之環境以避免聚集體或錯誤摺疊之蛋白質的形成，並且藉由低流速之操作，增加蛋白質於低尿素濃度區域之滯留時間，使變性蛋白質摺疊回具有活性、正確構型之復性蛋白質。 | zh_TW |
| dc.description.abstract | Misfolding and aggregation are the main obstacles of refolding efficiency in protein renaturation process. With the conventional dilution refolding method, large amount of refolding buffer is required to prevent aggregation and to dilute denaturant. In this investigation, via dialysis, besides midfolding and aggregates, reduced dithiothreitol (DTTred) was also proved to be a crucial factor in renaturation. Thus, we proposed a low dilution factor and high efficiency refolding strategy by adding high oxidized glutathione (GSSG) in the refolding buffer to react with carry-over DTTred, resulting in a 13 times of productivity and 60 % reduction of cost, compared to the conventional dilution refolding process.
Because of the benefit of separating DTTred in the beginning of renaturation, lysozyme could be refolded at high concentration by size exclusion chromatography (SEC). We further proposed the optimal volume of chaperon solvent plug and the loading time of sample to prevent aggregations before the column inlet. In addition, we also combined the chaperon solvent plug strategy, urea gradient strategy and step change of mobile phase flowrate strategy to offer an optimal SEC refolding environment. That is, at the beginning of injection, denatured lysozyme was loaded at a high flow rate with chaperon solvent plug. After denatured lyszoyme entered into the column inlet, low flow rate was adjusted to maintain enough retention time of denatured lysozyme in the urea gradient region and low urea concentration region to refold to active lysozyme. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:14:02Z (GMT). No. of bitstreams: 1 ntu-101-F95524088-1.pdf: 6958193 bytes, checksum: fb9a40031ada347a305d418228c22b6a (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 摘要……………………………………………………………………………………I
目錄………………………………………….……….………...…………………….III 圖目錄………………………..………………………………...…………………...VII 表目錄………………………………………………….….....……...……………...XII 第一章 緒論…………………………………………..………………………………1 1-1 研究背景與方向…………….………….…………………..………...……..1 1-2 論文內容…………………….……….……………………..………...……..2 第二章 文獻回顧………………………………….…………………………….……4 2-1 蛋白質工程………………….…..………………………..………...……..4 2-2 蛋白質變性..………………………………………………………………..6 2-3 蛋白質復性…………………………………………………………………9 2-4 蛋白質復性方法………………………………………………...…………15 2-4-1 直接稀釋復性法……………………………………………..…….15 2-4-2 大小排阻層析復性法………………………………………..…….19 2-4-3 透析復性法…………………………………………………..……27 2-5 溶菌酶……….……………………………………………………………30 2-5-1 溶菌酶簡介…………………………………...……………………30 2-5-2 溶菌酶結構………………………………………………………...34 2-5-3 溶菌酶活性..…………….…………………………………………34 第三章 實驗裝置、藥品與方法 ……………………………………………..….….38 3-1 實驗裝置…….…………………………………………………..…………38 3-2 實驗藥品…………………………………………………….……………..39 3-3 實驗方法……………………………………………………….…….….....41 3-3-1 實驗試劑製備..……..…………………………………………….....41 3-3-2 溶菌酶之活性測定……………………………………………….....43 3-3-2-1 分光光譜儀之偵測………..……………………………......43 3-3-2-2 連續波長分析儀之偵測………..……………….……….....43 3-3-3 溶菌酶之變性…………………………………………………….....43 3-3-4 溶菌酶之復性…………………………………………………….....44 3-3-4-1 直接稀釋復性法…………..………….……..………….…..44 3-3-4-2 大小排阻層析復性法…………………….……………….44 3-3-4-3 透析復性法…………………………….…………………..45 3-3-5 全波長測量...…………………….………………………………….46 3-3-6 氧化態DTT濃度之測量………………………………….…..…….46 3-3-7 經24小時變性後，變性蛋白質溶液中氧化態DTT濃度之測量….46 3-3-8 不可溶聚集體濃度之測量…...….………………………………….47 3-4 復性效率之定義…………………………………….……………….….....47 第四章 直接稀釋復性法..…..…………………………………………...……….....48 4-1 實驗動機…………………………………………………………...............48 4-2 直接稀釋法流程………………………………………….……………......48 4-3 復性系統中DTTs對蛋白質復性之影響…………….……………………52 4-3-1 變性24小時後之DTT………………………..……………………55 4-3-2 復性系統中之DTT…………………………………..……………59 4-3-3 復性系統中，DTTred濃度對蛋白質復性之影響……………..……64 4-4 高效率、低倍率之稀釋復性法…………………………………….………66 4-5 高效率稀釋復性法中之聚集體…………………………………….……..71 4-6 結論…………………………………………………………….…………..77 第五章 大小排阻層析復性法…………………………………………….…...........78 5-1實驗動機………………………………………………………...................78 5-2 實驗方法………………………………………………………...................79 5-2-1 高效能液相層析儀…………………………………………...........79 5-2-2 繞流系統……………………………………………………...........80 5-2-3 Chaperon solvent plug之操作程序………………………………....81 5-3 傳統SEC復性法及chaperon solvent plug復性法…………………….….83 5-4 注射閥至管柱前端之聚集體……………………………………….……..86 5-4-1 傳統復性法下，注射閥至管柱前端之聚集體…...………………..86 5-4-2 Chaperon solvent plug復性法對管柱前端聚集體之影響…..……..89 5-5 Chaperon solvent plug之設計對注射閥至管柱前端聚集體之影響……...90 5-6 Chaperon solvent plug之設計對蛋白質復性之影響………………….…..95 5-6-1 Chaperon solvent plug之設計對蛋白質質量回復率之影響…........97 5-6-2 Chaperon solvent plug之設計對蛋白質活性回復率之影響…..…100 5-7 流動相之更換對蛋白質復性之影響………………………….…………109 5-8結論………………………………………………….….…………………112 第六章 大小排阻層析管柱內復性環境對復性之影響………..…………………114 6-1實驗動機………………..………………………………….……...............114 6-2 SEC管柱中，蛋白質於不同尿素濃度區域之滯留時間之估算……........116 6-3 SEC管柱中，不同尿素濃度之復性環境對蛋白質復性之影響………....122 6-3-1不同chaperon solvent plug前段體積對SEC管柱內復性環境之影響..…………………………………..…………….….………………..…122 6-3-2 SEC管柱內，高尿素濃度環境對蛋白質復性之影響.…….……..124 6-3-3 chaperon solvent plug復性法中，管柱內低尿素濃度環境對蛋白質復性之影響……………………………..…………...…….…………..…133 6-3-4 SEC管柱內，蛋白質停滯於低尿素區域對復性之影響.…….…140 6-4 SEC管柱中，尿素梯度對蛋白質復性之影響……………………….....145 6-4-1實驗動機………………..……………….………………................145 6-4-2梯度復性法之操作程序…………..…….…………………............146 6-4-3尿素梯度復性法對管柱前端聚集體之影響……………...............146 6-4-4 SEC管柱內，尿素梯度對蛋白質復性之影響……………............148 6-4-5尿素梯度復性法中，管柱內低尿素濃度區域對蛋白質復性之影響.…………………………………..………………………………..….152 6-5結論………………………………………………….……….…………156 第七章 總結…..…………..……..……………………..………..…………………158 第八章 參考文獻………....……..……………………..………..…………………160 附錄A…………………………………………………………….…………………178 個人著作……………………………………………………….…………………...180 | |
| dc.language.iso | zh-TW | |
| dc.title | 溶菌酶復性程序效率提升之研究 | zh_TW |
| dc.title | The Improvement of Efficiency in the Lysozyme Renaturation Process | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.oralexamcommittee | 王勝仕(Steven Sheng-Shih Wang),蔡偉博(Wei-Bor Tsai),謝學真(Hsyue-Jen Hsieh),王孟菊(Meng-Jiy Wang),李振綱(Cheng-Kang Lee) | |
| dc.subject.keyword | 蛋白質復性,溶菌酶,直接稀釋法,大小排阻層析法,Chaperon solvent plug, | zh_TW |
| dc.subject.keyword | Protein renaturation,Lysozyme,Direct dilution,Size exclusion chromatography,Chaperon solvent plug, | en |
| dc.relation.page | 180 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-09 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
| 顯示於系所單位： | 化學工程學系 | |
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