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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張煥宗(Huan-Tsung Chang) | |
| dc.contributor.author | Yu-Ju Liao | en |
| dc.contributor.author | 廖玉茹 | zh_TW |
| dc.date.accessioned | 2021-06-17T00:11:44Z | - |
| dc.date.available | 2014-07-20 | |
| dc.date.copyright | 2012-07-20 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-11 | |
| dc.identifier.citation | Bond, G. C.; Semon, P. A. Gold Bull, 1973, 6, 102−105.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65786 | - |
| dc.description.abstract | 本論文分兩部分，第一部分是利用分子模板技術(Molecularly Imprinted Technology，MP)將凝血酶適合體(Thrombin-Binding Aptamer，TBA)修飾在金奈米粒子(Gold Nanoparticles，Au NPs)表面後，形成具有抗凝血酶與偵測凝血酶活性的適合體─金奈米粒子複合物(MP-TBA−Au NPs)；第二部份是利用金銀奈米棒(Gold-Silver Nanorods，Au/Ag NRs)修飾上肌動蛋白(Actin)，製備肌動蛋白─金銀奈米棒(Ac−Au/Ag NRs)」感測器於三磷酸腺苷(Adenosine-5'-triphosphate, ATP)的偵測。經由凝血酶凝集時間(thrombin clotting time，TCT)的測試，在最佳化的條件下，MP-TBA15/TBA29-T15−Au NPs可使TCT值延遲至160秒，優於其他抑制劑(如Argatroban (61 秒)、Hirudin (44秒)、Warfarin (40秒)、Heparin (34秒))。MP-TBA−Au NPs亦可應用於凝血酶的偵測，利用凝血酶分子同時與MP-TBA15−Au NPs和MP-TBA29−Au NPs作用產生聚集現象，在模擬的生理環境偵測凝血酶分子，其線性範圍為1.0−10 nM (R2 = 0.98)。MP-TBA−Au NPs對於凝血酶有極高的選擇性與高穩定性，可應用於真實樣品(例如:人類血清以及血漿)中凝血酶的抑制及偵測。第二部分是利用Ac−Au/Ag NRs偵測樣品中ATP濃度，因ATP的存在會使肌動蛋白單體(G-Actin)產生聚合作用(polymerization)形成絲狀肌動蛋白聚合體(F-actin)進而造成Au/Ag NRs的聚集，藉由測量金銀奈米棒的吸收值比(Ex900/730；吸收值波長900 nm與730 nm比值)即可得知金銀奈米棒聚集的程度，進而定量出樣品中ATP濃度。Ac−Au/Ag NRs在模擬的生理環境中對於ATP偵測的線性範圍為50 nM−1.0 μM (R2 = 0.99)。Ac−Au/Ag NRs對ATP、ADP、AMP以和Adenosine有很好的選擇性，可應用於複雜的樣品(如人類血漿)中偵測ATP濃度。 | zh_TW |
| dc.description.abstract | There are two parts in this thesis: the first part is focused on that we prepared thrombin-binding aptamer-conjugated gold nanoparticles (TBA−Au NPs) through a molecularly imprinted (MP) approach, which provided highly efficient inhibition activity toward the polymerization of fibrinogen; another is to prepared actin-conjugated gold-silver nanorods (Ac−Au/Ag NRs) to measure Adenosine-5'-triphosphate (ATP) concentration in the aqueous solution by colorimetric method. MP-TBA15/TBA29-T15−Au NPs provided the highest binding affinity toward thrombin with a dissociation constant of 5.2 × 10−11 M. Finally, we tested the thrombin clotting time (TCT) measurements in plasma samples and found that MP-TBA15/TBA29-T15−Au NPs had greater anticoagulation activity than heparin, argatroban, hirudin and warfarin. In addition, we demonstrated that thrombin induced the formation of aggregates from MP-TBA15-T15−Au NPs and MP-TBA29-T15−Au NPs, thereby allowing the colorimetric detection of thrombin at the nanomolar level in serum samples. The second part is to demonstrated a colorimetric sensing approach for the determination of the concentration of adenosine-5'-triphosphate (ATP) using actin-conjugated gold nanorods (Ac−Au/Ag NRs). In the presence of the ATP, the color of the Ac−Au/Ag NRs solution changed from red to purple as a result of actin polymerization. For mixtures of ATP, polymerization buffer (40.0 mM NaCl and 2.0 mM MgCl2) and physiological buffer (25 mM Tris-HCl (pH 7.4), 150 mM NaCl, 5.0 mM KCl, 1.0 mM MgCl2, 1.0 mM CaCl2), a linear correlation (R2=0.99) existed between the ratio of the extinctions of the Ac−Au NRs at 900 and 730 nm (Ex900/730) and the concentration of ATP. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-17T00:11:44Z (GMT). No. of bitstreams: 1 ntu-101-R99223209-1.pdf: 3469839 bytes, checksum: 6d8330cc3b2635a1fb1a8ea008263ab4 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 中文摘要 I
英文摘要 II 目錄 III 圖表目錄 VI 第一章 緒論 1 1.1金奈米材料 1 1.2 分子模板技術 3 1.2.1分子模板技術發展歷史 3 1.2.2分子模板製備方法 4 1.2.3 共價鍵結法與非共價鍵結法 4 1.2.4 分子模板技術應用 5 1.3 凝血酶簡介 5 1.4抗凝血酶核酸適合體介紹 6 1.5肌動蛋白簡介 8 1.6研究動機 9 1.7本章圖表 10 1.8參考文獻 17 第二章 應用分子模板之適合體/金奈米粒子複合材料於凝血酶活性抑制與偵測 21 2.1 前言 21 2.2 實驗材料與方法 22 2.2.1 實驗試藥 22 2.2.2 金奈米粒子合成 23 2.2.3 利用分子模板技術合成適合體−金奈米粒子複合物 24 2.2.4 凝集動力學即時檢測 25 2.2.5 測定適合體−金奈米粒子複合物與凝血酶解離常數 25 2.2.6 凝血酶凝集時間測定 26 2.2.7凝血酶偵測 26 2.2.8選擇性測試 27 2.3 實驗結果與討論 27 2.3.1 MP-TBA15/TBA29-T15–Au NPs合成機制 27 2.3.2分子模板技術對抑制效率的探討 28 2.3.3模板蛋白濃度對抑制效果的探討 29 2.3.4適合體與金奈米粒子間距離對抑制效率的探討 30 2.3.5適合體密度對抑制效率的探討 30 2.3.6適合體−金奈米粒子複合物對凝血酶凝集時間(TCT)的影響 31 2.3.7適合體−金奈米粒子複合物對凝血酶偵測偵測靈敏度 31 2.4 結論 32 2.5 本章圖表 34 2.6 參考文獻 50 第三章 金銀奈米棒修飾上肌動蛋白應用於三磷酸腺苷之偵測 52 3.1前言 52 3.2實驗材料與方法 53 3.2.1實驗試藥 53 3.2.2金銀奈米棒合成 54 3.2.3合成肌動蛋白−金奈米材料複合物 54 3.2.4選擇性測試 55 3.2.5肌動蛋白−金銀奈米棒偵測ATP 55 3.3 實驗結果與討論 56 3.3.1肌動蛋白−金銀奈米棒偵測三磷酸腺苷機制 56 3.3.2不同金奈米材料對於偵測靈敏性之影響 56 3.3.3不同濃度肌動蛋白對於肌動蛋白−金銀奈米棒複合物穩定性以及偵測靈敏性影響 57 3.3.4肌動蛋白−金銀奈米棒複合物選擇性探討 57 3.3.5肌動蛋白−金銀奈米棒複合物應用於樣品ATP偵測 58 3.4 結論 58 3.5 本章圖表 60 3.6 參考文獻 70 | |
| dc.language.iso | zh-TW | |
| dc.subject | 金奈米材料 | zh_TW |
| dc.subject | 適合體 | zh_TW |
| dc.subject | 分子模板技術 | zh_TW |
| dc.subject | 凝血酶 | zh_TW |
| dc.subject | 三磷酸腺苷 | zh_TW |
| dc.subject | gold nanomaterial | en |
| dc.subject | aptamer | en |
| dc.subject | molecularly imprinted technology | en |
| dc.subject | thrombin | en |
| dc.subject | ATP | en |
| dc.title | 利用功能性金奈米材料抑制凝血酶活性及偵測三磷酸腺苷 | zh_TW |
| dc.title | Functional gold nanomaterials for the inhibition of the activity of thrombin and for the detection of ATP | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 黃志清,吳秀梅,黃郁棻 | |
| dc.subject.keyword | 金奈米材料,適合體,分子模板技術,凝血酶,三磷酸腺苷, | zh_TW |
| dc.subject.keyword | gold nanomaterial,aptamer,molecularly imprinted technology,thrombin,ATP, | en |
| dc.relation.page | 71 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-12 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
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