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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 謝旭亮(Hsu-Liang Hsieh) | |
dc.contributor.author | Jin-Long Wu | en |
dc.contributor.author | 吳金龍 | zh_TW |
dc.date.accessioned | 2021-06-16T23:57:25Z | - |
dc.date.available | 2017-07-27 | |
dc.date.copyright | 2012-07-27 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-17 | |
dc.identifier.citation | 李德政 (2006) Identification of blue light-induced genes involved in the lycopene accumulation of Lycopersicon esculentum fruit. 選殖番茄果實中受藍光誘導而影響茄紅素累積之相關基因,碩士論文,植物科學研究所,臺灣大學,台北。
曾鈺媛(2010) Functional study of a light-induced factor TDR4 affecting lycopene levels in tomato fruit. 受光調控因子 TDR4 影響番茄果實中 茄紅素含量之功能性研究,碩士論文,植物科學研究所,臺灣大學,台北。 Agarwal, S., and Rao, A.V. (2000). Tomato lycopene and its role in human health and chronic diseases. CMAJ 163: 739-744. Alba, R., Cordonnier-Pratt, M.M., and Pratt, L.H. (2000). Fruit-localized phytochromes regulate lycopene accumulation independently of ethylene production in tomato. Plant Physiol 123: 363-370. Alba, R., Payton, P., Fei, Z., McQuinn, R., Debbie, P., Martin, G.B., Tanksley, S.D., and Giovannoni, J.J. (2005). Transcriptome and selected metabolite analyses reveal multiple points of ethylene control during tomato fruit development. Plant Cell 17: 2954-2965. Alexander, L., and Grierson, D. (2002). Ethylene biosynthesis and action in tomato: a model for climacteric fruit ripening. J Exp Bot 53: 2039-2055. Barry, C.S., McQuinn, R.P., Thompson, A.J., Seymour, G.B., Grierson, D., and Giovannoni, J.J. (2005). Ethylene insensitivity conferred by the Green-ripe and Never-ripe 2 ripening mutants of tomato. Plant Physiol 138: 267-275. Becker, A., and Theissen, G. (2003). The major clades of MADS-box genes and their role in the development and evolution of flowering plants. Mol Phylogenet Evol 29: 464-489. Beecher, G.R. (1998). Nutrient content of tomatoes and tomato products. Proc Soc Exp Biol Med 218: 98-100. Briza, J., Pavingerova, D., Prikrylova, P., Gazdova, J., Vlasak, J., and Niedermeierova, H. (2008). Use of phosphomannose isomerase-based selection system for Agrobacterium-mediated transformation of tomato and potato. Biol Plantarum 52: 453-461. Busi, M.V., Bustamante, C., D'Angelo, C., Hidalgo-Cuevas, M., Boggio, S.B., Valle, E.M., and Zabaleta, E. (2003). MADS-box genes expressed during tomato seed and fruit development. Plant Mol Biol 52: 801-815. Cardon, G.H., Hohmann, S., Nettesheim, K., Saedler, H., and Huijser, P. (1997). Functional analysis of the Arabidopsis thaliana SBP-box gene SPL3: a novel gene involved in the floral transition. Plant J 12: 367-377. Chen, D.C., Yang, B.C., and Kuo, T.T. (1992). One-step transformation of yeast in stationary phase. Curr Genet 21: 83-84. Cunningham, F.X. (2002). Regulation of carotenoid synthesis and accumulation in plants. Pure Appl Chem 74: 1409-1417. Cunningham, F.X., and Gantt, E. (1998). Genes and enzymes of carotenoid biosynthesis in plants. Annu Rev Plant Physiol Plant Mol Biol 49: 557-583. de Folter, S., and Angenent, G.C. (2006). trans meets cis in MADS science. Trends Plant Sci 11: 224-231. Dumas, Y., Dadomo, M., Di Lucca, G., and Grolier, P. (2003). Effects of environmental factors and agricultural techniques on antioxidant content of tomatoes. J Sci Food Agr 83: 369-382. Eriksson, E.M., Bovy, A., Manning, K., Harrison, L., Andrews, J., De Silva, J., Tucker, G.A., and Seymour, G.B. (2004). Effect of the Colorless non-ripening mutation on cell wall biochemistry and gene expression during tomato fruit development and ripening. Plant Physiol 136: 4184-4197. Ferrandiz, C., Liljegren, S.J., and Yanofsky, M.F. (2000). Negative regulation of the SHATTERPROOF genes by FRUITFULL during Arabidopsis fruit development. Science 289: 436-438. Fraser, P.D., Truesdale, M.R., Bird, C.R., Schuch, W., and Bramley, P.M. (1994). Carotenoid biosynthesis during tomato fruit development (Evidence for tissue-specific gene expression). Plant Physiol 105: 405-413. Fujisawa, M., Nakano, T., and Ito, Y. (2011). Identification of potential target genes for the tomato fruit-ripening regulator RIN by chromatin immunoprecipitation. BMC Plant Biol 11: 26. Giliberto, L., Perrotta, G., Pallara, P., Weller, J.L., Fraser, P.D., Bramley, P.M., Fiore, A., Tavazza, M., and Giuliano, G. (2005). Manipulation of the blue light photoreceptor cryptochrome 2 in tomato affects vegetative development, flowering time, and fruit antioxidant content. Plant Physiol 137: 199-208. Giovannoni, J.J. (2004). Genetic regulation of fruit development and ripening. Plant Cell 16 Suppl: S170-180. Gramzow, L., and Theissen, G. (2010). A hitchhiker's guide to the MADS world of plants. Genome Biol 11. Gramzow, L., Ritz, M.S., and Theissen, G. (2010). On the origin of MADS-domain transcription factors. Trends Genet 26: 149-153. Gu, Q., Ferrandiz, C., Yanofsky, M.F., and Martienssen, R. (1998). The FRUITFULL MADS-box gene mediates cell differentiation during Arabidopsis fruit development. Development 125: 1509-1517. Hernandez, J.M., Feller, A., Morohashi, K., Frame, K., and Grotewold, E. (2007). The basic helix-loop-helix domain of maize R links transcriptional regulation and histone modifications by recruitment of an EMSY-related factor. P Natl Acad Sci USA 104: 17222-17227. Hong, R.L., Hamaguchi, L., Busch, M.A., and Weigel, D. (2003). Regulatory elements of the floral homeotic gene AGAMOUS identified by phylogenetic footprinting and shadowing. Plant Cell 15: 1296-1309. Ito, Y., Kitagawa, M., Ihashi, N., Yabe, K., Kimbara, J., Yasuda, J., Ito, H., Inakuma, T., Hiroi, S., and Kasumi, T. (2008). DNA-binding specificity, transcriptional activation potential, and the rin mutation effect for the tomato fruit-ripening regulator RIN. Plant J 55: 212-223. Khudairi, A.K., and Arboleda, O.P. (1971). Phytochrome-mediated carotenoid biosynthesis and its influence by plant hormones. Physiol Plantarum 24: 18-&. Klee, H.J., and Giovannoni, J.J. (2011). Genetics and control of tomato fruit ripening and quality attributes. Annu Rev Genet 45: 41-59. Lee, J.M., Joung, J.G., McQuinn, R., Chung, M.Y., Fei, Z., Tieman, D., Klee, H., and Giovannoni, J. (2012). Combined transcriptome, genetic diversity and metabolite profiling in tomato fruit reveals that the ethylene response factor SlERF6 plays an important role in ripening and carotenoid accumulation. Plant J 70: 191-204. Leseberg, C.H., Eissler, C.L., Wang, X., Johns, M.A., Duvall, M.R., and Mao, L. (2008). Interaction study of MADS-domain proteins in tomato. J Exp Bot 59: 2253-2265. Levin, I., Frankel, P., Gilboa, N., Tanny, S., and Lalazar, A. (2003). The tomato dark green mutation is a novel allele of the tomato homolog of the DEETIOLATED1 gene. Theoretical and Applied Genetics 106: 454-460. Liang, X., Nazarenus, T.J., and Stone, J.M. (2008). Identification of a consensus DNA-binding site for the Arabidopsis thaliana SBP domain transcription factor, AtSPL14, and binding kinetics by surface plasmon resonance. Biochemistry 47: 3645-3653. Lieberman, M., Segev, O., Gilboa, N., Lalazar, A., and Levin, I. (2004). The tomato homolog of the gene encoding UV-damaged DNA binding protein 1 (DDB1) underlined as the gene that causes the high pigment-1 mutant phenotype. Theor Appl Genet 108: 1574-1581. Lin, Z., Hong, Y., Yin, M., Li, C., Zhang, K., and Grierson, D. (2008). A tomato HD-Zip homeobox protein, LeHB-1, plays an important role in floral organogenesis and ripening. Plant J 55: 301-310. Lozano, R., Gimenez, E., Cara, B., Capel, J., and Angosto, T. (2009). Genetic analysis of reproductive development in tomato. Int J Dev Biol 53: 1635-1648. Lu, S., and Li, L. (2008). Carotenoid metabolism: biosynthesis, regulation, and beyond. J Integr Plant Biol 50: 778-785. Manning, K., Tor, M., Poole, M., Hong, Y., Thompson, A.J., King, G.J., Giovannoni, J.J., and Seymour, G.B. (2006). A naturally occurring epigenetic mutation in a gene encoding an SBP-box transcription factor inhibits tomato fruit ripening. Nat Genet 38: 948-952. Mardis, E.R. (2007). ChIP-seq: welcome to the new frontier. Nat Methods 4: 613-614. Martel, C., Vrebalov, J., Tafelmeyer, P., and Giovannoni, J.J. (2011). The tomato MADS-box transcription factor RIPENING INHIBITOR interacts with promoters involved in numerous ripening processes in a COLORLESS NONRIPENING-dependent manner. Plant Physiol 157: 1568-1579. Mustilli, A.C., Fenzi, F., Ciliento, R., Alfano, F., and Bowler, C. (1999). Phenotype of the tomato high pigment-2 mutant is caused by a mutation in the tomato homolog of DEETIOLATED1. Plant Cell 11: 145-157. Penuelas, J., and Munne-Bosch, S. (2005). Isoprenoids: an evolutionary pool for photoprotection. Trends Plant Sci 10: 166-169. Pnueli, L., Abu-Abeid, M., Zamir, D., Nacken, W., Schwarz-Sommer, Z., and Lifschitz, E. (1991). The MADS box gene family in tomato: temporal expression during floral development, conserved secondary structures and homology with homeotic genes from Antirrhinum and Arabidopsis. Plant J 1: 255-266. Pramila, T., Miles, S., GuhaThakurta, D., Jemiolo, D., and Breeden, L.L. (2002). Conserved homeodomain proteins interact with MADS box protein Mcm1 to restrict ECB-dependent transcription to the M/G1 phase of the cell cycle. Gene Dev 16: 3034-3045. Quail, P.H. (2002). Photosensory perception and signalling in plant cells: new paradigms? Curr Opin Cell Biol 14: 180-188. Salinas, M., Xing, S., Hohmann, S., Berndtgen, R., and Huijser, P. (2012). Genomic organization, phylogenetic comparison and differential expression of the SBP-box family of transcription factors in tomato. Planta 235: 1171-1184. Schroeder, D.F., Gahrtz, M., Maxwell, B.B., Cook, R.K., Kan, J.M., Alonso, J.M., Ecker, J.R., and Chory, J. (2002). De-etiolated 1 and damaged DNA binding protein 1 interact to regulate Arabidopsis photomorphogenesis. Curr Biol 12: 1462-1472. Schwarz-Sommer, Z., Huijser, P., Nacken, W., Saedler, H., and Sommer, H. (1990). Genetic control of flower development by homeotic genes in antirrhinum majus. Science 250: 931-936. Sigareva, M., Spivey, R., Willits, M.G., Kramer, C.M., and Chang, Y.F. (2004). An efficient mannose selection protocol for tomato that has no adverse effect on the ploidy level of transgenic plants. Plant Cell Rep 23: 236-245. Sprenkle, A.B., Murray, S.F., and Glembotski, C.C. (1995). Involvement of multiple cis elements in basal- and alpha-adrenergic agonist-inducible atrial natriuretic factor transcription. Roles for serum response elements and an SP-1-like element. Circ Res 77: 1060-1069. Tieman, D.M., Taylor, M.G., Ciardi, J.A., and Klee, H.J. (2000). The tomato ethylene receptors NR and LeETR4 are negative regulators of ethylene response and exhibit functional compensation within a multigene family. Proc Natl Acad Sci U S A 97: 5663-5668. Todd, R., and Tague, B.W. (2001). Phosphomannose isomerase: A versatile selectable marker for Arabidopsis thaliana germ-line transformation. Plant Mol Biol Rep 19: 307-319. Vrebalov, J., Ruezinsky, D., Padmanabhan, V., White, R., Medrano, D., Drake, R., Schuch, W., and Giovannoni, J. (2002). A MADS-box gene necessary for fruit ripening at the tomato ripening-inhibitor (rin) locus. Science 296: 343-346. Zhang, M., Yuan, B., and Leng, P. (2009). The role of ABA in triggering ethylene biosynthesis and ripening of tomato fruit. J Exp Bot 60: 1579-1588. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65667 | - |
dc.description.abstract | 在過去的研究中對於光照處理可以誘導番茄果實中茄紅素的累積已經是廣為人知的現象,但是光是藉由光訊息傳遞下的哪些因子與這些因子是如何去影響茄紅素的累積到目前為止還不太明瞭。
本實驗室在過去利用不同品系的番茄果實照射不同光源後比較茄紅素累積的情形,並以抑制扣減式雜交(suppression subtractive hybridization)得到了一些可受到藍光誘導且可能參與調控茄紅素累積的候選基因。我們對於其中一個候選基因MADS轉錄因子TDR4感到高度興趣。並在先前得到了TDR4降低表現的轉殖株,發現當果實中TDR4表現降低時,類胡蘿蔔素生合成途徑中的關鍵酵素PSY的表現量也會有降低的趨勢,且果實會呈現茄紅素含量降低的外觀。因此我們推測TDR4可直接在轉錄層次上影響PSY的表現並正向影響茄紅素累積。而TDR4是否真的會調控PSY,以及TDR4是否有其他生理調控的功能是值得探討的。 在本次研究當中,我們利用酵母菌單雜交(Yeast one-hybrid)、膠體電泳位移分析(electrophoretic mobility shift assay)與染色質絲免疫共沉澱(Chromatin immunoprecipitation)的方法,證明TDR4可直接結合於PSY啟動子與內插子區域。此外我們也在染色質絲免疫共沉澱的實驗當中,發現TDR4除PSY之外也可結合於一些果實成熟中的重要基因,像CNR、ACS4、PG2a、PDS與TDR4本身的啟動子區域。進一步我們建立了以TDR4自身啟動子表現TDR4的大量表現轉殖株。藉由偵測野生型、TDR4大量表現與TDR4降低表現轉殖株果實中,TDR4與受TDR4調控基因的表現,我們發現TDR4可正向調控這些果實成熟與類胡蘿蔔素生合成相關基因的表現量。而TDR4大量表現轉殖株的紅熟期果實相較起野生型的紅熟期果實有著較高的茄紅素含量,且TDR4大量表現轉殖株的綠熟期果實對於藍光照射之下累積茄紅素的反應比野生型也較強烈。綜合以上實驗數據,我們認為TDR4對於照光之下番茄果實累積茄紅素為一項重要的調控因子,且TDR4很可能也參與在果實成熟的其他作用當中。 | zh_TW |
dc.description.abstract | It is well known that light treatment can induce the lycopene accumulation in tomato fruit, but which factors in light signaling and how these factors regulate this process are still unknown. In our previous studies, we compared the fruits of different tomato species that were treated with different light, and identified several gene candidates by suppression subtractive hybridization assay, which may be involved in the regulation of lycopene accumulation. Among them, one candidate is MADS-box transcription factor TDR4. We generated the reduction-of-function of TDR4 transgenic plants previously, and found that the expression levels of TDR4 were decreased, leading to a reduction of the levels of PSY, a key enzyme gene of the carotenoid biosynthesis. Besides, the fruit of the reduction-of-function of TDR4 transgenic plants contains a decreased level of the lycopene. So TDR4 may positively regulate the expression of PSY transcripts to modulate the lycopene accumulation. Thus, we are wondering whether TDR4 can directly regulate the expression of PSY, and whether TDR4 has other physiological functions. In this study, we used yeast one-hybrid, electrophoretic mobility shift assay (EMSA) and chromatin immunoprecipitation (ChIP) assays to prove that TDR4 can directly bind to the promoter and the intron region of PSY. Besides, we also found that TDR4 can bind to the promoters of some fruit ripening related genes such as CNR, ACS4, PG2a, PDS and TDR4 itself by ChIP assay. Furthermore, we generated the TDR4 promoter-driven gain-of-function of TDR4 transgenic plants. By analyzing the expression levels of TDR4 and TDR4-regulated genes in the fruits of wild type, gain-of-function and reduction-of-function of TDR4 transgenic lines, we found that TDR4 positively regulated the ripening-related and carotenoid biosynthetic genes. Moreover, the lycopene levels in the gain-of-function of TDR4 transgenic lines are higher than wild type, and the mature green fruit of the gain-of-function of TDR4 transgenic lines is more sensitive to blue light, resulting in more lycopene accumulation. Taken together, these data reveal that TDR4 is a key component in light-induced accumulation of lycopene, and may be also involved in fruit ripening. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:57:25Z (GMT). No. of bitstreams: 1 ntu-101-R99b42029-1.pdf: 2387590 bytes, checksum: aa9dd5cb709c7a20c7886d0de53b64eb (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 中文摘要...........................................................................................................................1
英文摘要...........................................................................................................................2 前言 一、番茄簡介…………………………………………………………………..……..4 二、植物體中類胡蘿蔔素之功能與生合成................................................................5 三、光參與在茄紅素累積之相關研究…....................................................................5 四、MADS轉錄因子簡介...........................................................................................7 五、可影響茄紅素累積之轉錄因子與其調控關係....................................................8 六、研究動機…………………………………………………………………………8 七、研究策略 (1)證明TDR4可直接結合在PSY啟動子區域……………………..…………………….……….……….9 (2)找尋TDR4所調控之基因,以及此調控關係具有何種生理意義………………….....9 (3)建立以TDR4自身啟動子大量表現TDR4之轉植株觀察其外表型與基 因表現………………………………………………………………….……..10 材料與方法 一、植物材料與生長條件………………………………………….…...…..11 二、酵母菌單雜交(Yeast one-hybrid)…………………………………….………..11 三、Electrophoretic mobility shift assay (EMSA)………………………….………..11 四、番茄葉片原生質體分離與轉染……………………………………….……….12 五、染色質絲免疫共沉澱(Chromatin immuneprecipitation)………………………12 六、番茄轉殖………………………………………………………………………..12 七、總RNA萃取與基因表現測定………………………………………………...12 八、茄紅素測定……………………………………………………………………..13 九、果實照光處理…………………………………………………………………..13 結果 一、 TDR4可直接與PSY啟動子上之MEF2-like CArG序列做結合………....14 二、 TDR4可結合於PSY基因的啟動子與內插子……………………....……..14 三、 TDR4可與許多果實成熟相關基因的啟動子結合…………………….…..15 四、 TDR4啟動子區域含有許多受光調控之順勢因子…………………….…..16 五、 以TDR4自身啟動子驅動TDR4之轉植株造成TDR4表現量上升….…..16 六、 受TDR4所調控的基因與TDR4之表現量呈現高度正相關……………..17 七、 TDR4大量表現轉植株中,成熟果實含有較高的茄紅素含量……………17 八、 TDR4大量表現轉植株之果實對於藍光反應更加敏感…………………..,18 九、 TDR4大量表現對於花、花萼與葉片的型態無明顯改變…………………18 討論 一、 TDR4結合順勢因子之探討……………………………………………..…19 二、 TDR4正向調控許多和果實成熟相關之基因…………………………...…20 三、 TDR4可能同時藉由直接或間接的途徑去調控果實茄紅素含量………...21 四、 TDR4、RIN與CNR也許以複合體形式調控下游基因表現…….………21 五、 離層酸(ABA)與茄紅素和乙烯之關係探討………………………………..22 六、 總結………………………………………………………………………….22 七、 未來工作建議……………………………………………………………….22 圖表.................................................................................................................................24 參考文獻.........................................................................................................................38 附錄一、實驗流程………..............................................................................................43 附錄二、番茄種子消毒與種植.....................................................................................53 附錄三、實驗原理簡介………………………………………………………………..54 附圖與附表.....................................................................................................................56 | |
dc.language.iso | zh-TW | |
dc.title | 番茄果實中轉錄因子TDR4調控茄紅素累積之分子機制研究 | zh_TW |
dc.title | Functional study of the molecular mechanism underlying transcription factor TDR4-regulated lycopene accumulation in tomato fruit | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 葉開溫(Kai-Wun Yeh),鄭秋萍(Chiu-Ping Cheng),王淑珍(Shu-Jen Wang),陳仁治(Chen, Jen-Chih) | |
dc.subject.keyword | TDR4,茄紅素,番茄果實,乙烯, | zh_TW |
dc.subject.keyword | TDR4,lycipene,tomato fruit,ethylene, | en |
dc.relation.page | 62 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-18 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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