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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Jhen-Cen Lin | en |
| dc.contributor.author | 林珍岑 | zh_TW |
| dc.date.accessioned | 2021-06-16T23:50:49Z | - |
| dc.date.available | 2015-10-16 | |
| dc.date.copyright | 2012-07-30 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-20 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65565 | - |
| dc.description.abstract | 本研究以沾筆式奈米微影術 (Dip-Pen Nanolithgraphy, DPN) 在3-甘油基丙基三甲基矽烷氧 (3-glycidoxypropyltrimethoxysila, GPTS) 修飾之玻璃基材上製造鏈霉親和素異-硫氰酸螢光素 (streptavidin-FITC) 之蛋白質奈米陣列 (protein nanoarray )，探討蛋白質墨水性質以及探針駐留時間 (dwell time) 對製程結果的影響。本實驗製備兩種蛋白質墨水：Streptavidin-FITC分別溶於正常磷酸生理緩衝液 (1xPhosphate buffer saline, 1x PBS) 與市售聚合物載體。研究結果顯示，墨水性質對蛋白質點直徑大小、點直徑變異以及墨水消耗速度有顯著的影響，提高墨水黏滯性可減少點直徑變異並減少墨水消耗速度，藉此提升DPN製程的穩定性。控制探針駐留時間可製造不同直徑的蛋白質點。當探針駐留時間為一秒時，溶於1x PBS的streptavidin-FITC墨水製造之點直徑為4.24 ± 0.85 um；溶於市售聚合物載體的streptavidin-FITC墨水製造之點直徑為7.43 ± 0.30 um。另外，利用溶於聚合物之streptavidin-FITC墨水製造之蛋白質奈米陣列的產量為每分鐘300個點，點陣列密度約為每平方微米13000個點。本研究結果探討各項參數對製程的影響，並提升DPN之蛋白質奈米陣列製程的穩定性。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-16T23:50:49Z (GMT). No. of bitstreams: 1 ntu-101-R99548031-1.pdf: 12176812 bytes, checksum: b7238f687149ff8f9fe3dfca7f985f0f (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii CONTENTS iv LIST OF FIGURES vi LIST OF TABLES x Chapter 1 Introduction 1 1.1 Motivation 1 1.2 Research niche and purpose 3 1.3 Thesis structure 4 Chapter 2 Literature review 5 2.1 Protein nanoarray 5 2.1.1 Importance of protein nanoarray 5 2.1.2 Techniques fabricating protein nanopatterns 7 2.2 DPN generated protein nanoarray 14 2.2.1 Applications for biomolecules 14 2.2.2 Lithography procedure 18 2.2.3 Protein immobilization methods 21 2.2.4 Protein patterning strategy via DPN 24 2.2.5 Limitation of current research on direct DPN 29 2.3 Parameters of direct DPN 30 2.4 AFM surface scanning 34 2.5 Contact angle measurement 35 Chapter 3 Material and methods 37 3.1 Experiment design 37 3.2 Materials and methods 38 3.2.1 Substrate preparation 38 3.2.2 Preparation of protein ink 39 3.2.3 DPN process 40 3.2.4 Measurement system 42 3.2.5 Statistical methods 42 Chapter 4 Discussion and results 43 4.1 Chemical property of GPTS-coated glass 43 4.2 Effect of Ink property on dot sizes 45 4.3 Effect of dwell time on dot sizes 50 4.4 Effect of ink property and dwell time on ink consumption rate 55 4.5 Production yield 57 Chapter 5 Conclusions 58 5.1 Conclusion 58 5.2 Limitation of the study 59 5.3 Future work 59 5.4 Perspective 60 REFERENCE 63 | |
| dc.language.iso | en | |
| dc.title | 以沾筆式奈米微影術於玻璃基材上製造蛋白質奈米陣列 | zh_TW |
| dc.title | Direct Deposition of Protein Nanoarray onto Glass Substrates via Dip-Pen Nanolithography | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李世光(Chih-Kung Lee),黃義侑(Yi-You Huang),林致廷(Chih-Ting Lin) | |
| dc.subject.keyword | 沾筆式奈米微影術,蛋白質奈米陣列,蛋白質墨水性質,駐留時間, | zh_TW |
| dc.subject.keyword | Dip-pen nanolithography,protein nanoarray,ink property,dwell time, | en |
| dc.relation.page | 66 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-20 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| 顯示於系所單位： | 醫學工程學研究所 | |
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