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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭石通(Shih-Tong Jeng) | |
dc.contributor.author | Liang-Yu Hou | en |
dc.contributor.author | 侯良諭 | zh_TW |
dc.date.accessioned | 2021-06-16T23:49:08Z | - |
dc.date.available | 2017-07-26 | |
dc.date.copyright | 2012-07-26 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-23 | |
dc.identifier.citation | Alonso-Ramı’rez, A., Rodrı’guez, D., Reyes, D., Jime’nez, J.s.A., Nicola’s, G., Lo’pez-Climent, M.a., Go’mez-Cadenas, A., and Nicola’s, C. (2009) . Evidence for a role of gibberellins in salicylic acid-modulated early plant responses to abiotic stress in Arabidopsis seeds. Plant Physiol. 150: 1335-1344.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65537 | - |
dc.description.abstract | 熱逆境會對植物生長造成不利的影響,因此研究植物體內耐熱的基因是一項
很重要的課題。利用微陣列分析技術,比較正常生長與處理熱逆境的阿拉伯芥基 因的表現差異,選取兩個轉錄因子 AtWRKY54 (AT2G40750)與 AtWRKY70 (AT3G56400)。此二基因的表現量均受到熱逆境所抑制。利用此基因缺失與大量表 現的轉殖株做一連串的生理分析。偵測植物體內過氧化氫累積的情形,大量表現 AtWRKYY54 有助於減低因熱所產生的過氧化氫累積,而在大量表現 AtWRKY70 的轉殖株當中則無此現象,但若此基因發生缺失則會使過氧化氫在常溫或高溫下有 不正常累積的情形。進一步分析植物體內部份抗氧化酵素的活性是否有受到影響, 並無觀察到明顯的差異。高溫逆境下常會伴隨著脂質的過氧化產生有毒物質丙二 醛,不利植物的正常生理代謝,因此利用偵測丙二醛累積的情形判斷植株受熱逆 境的傷害程度,AtWRKY54 與 AtWRKY70 大量表現的轉殖株中,丙二醛累積量皆有明顯的下降。在植物耐熱性測試方面,大量表現 AtWRKY54 可以使植株在熱逆境的情況下有較好的生長與存活能力,大量表現 ATWRKY70 無明顯差異,但此基因缺失會造成植物基礎的耐熱能力下降。綜合以上結果,AtWRKY54 可以減少過氧化氫的累積與穩定細胞膜構造,使植物在熱逆境下可以獲得比較好耐熱性,而 ATWRKY70 的效果則比較次要。 | zh_TW |
dc.description.abstract | Heat stress causes severe damage in plants. We used microarray to analyze the difference of gene expression between heat and non-heat treated Arabidopsis. Two heat
stress related transcription factors, AtWRKY54 (AT2G40750) and AtWRKY70 (AT3G56400) were chosen for further study. The expression levels of AtWRKY54 and AtWRKY70 reduced in heat stress. Over-expression AtWRKY54 would decrease hydrogen peroxide accumulation that caused by heat stress. No obvious effect exists in AtWRKY70 overexpression plants, but defect in AtWRKY70 could increase hydrogen peroxide accumulation. However, AtWRKY54 and AtWRKY70 did not participate in the regulation of antioxidant enzymes. In addition, AtWRKY54 and AtWRKY70 could stabilize cell membrane during heat stress. Over-expressing AtWRKY54 further enhanced hypocotyl growth and showed higher survival rate than that of wild type after heat shock. Over-expressing AtWRKY70 revealed better hypocotyl growth than wild type. Conclusively, AtWRKY54 might decrease ROS generation and stabilize cell membrane in heat stress. AtWRKY70 could not decrease ROS generation, but it may still involve in cell membrane stability. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:49:08Z (GMT). No. of bitstreams: 1 ntu-101-R99b42007-1.pdf: 2391440 bytes, checksum: 6e0cdd4c824d6c58a732892e8c278d31 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 目錄
謝誌…………………………………………………………………………………..Ι 中文摘要…………………………………………………………………………….ΙΙ 英文摘要……………………………………………………………………….............ΙΙΙ 縮寫對照表…………………………………………………………………………...ΙV 目錄…………………………………………………………………………………...V 圖表目錄……………………………………………………………………………VII 第一章 前言…………………………………………………………………………1 1、熱逆境………………………………………………………………………..1 2、熱逆境對植物的影響...............1 3、植物抵禦熱逆境之機制........................2 4、阿拉伯芥耐熱基因的釣取…………………………………………………...7 5、WRKY轉錄因子…………………………………………………………….7 第二章 材料與方法…………………………………………………………………11 1、材料………………………………………………………………………….11 2、阿拉伯芥葉片組織DNA萃取.................11 3、阿拉伯芥葉片組織RNA萃取..........................12 4、聚合酶連鎖反應 (PCR)與即時定量聚合酶連鎖反應 (quantitative real-time PCR).....................12 5、質體構築..............................13 6、農桿菌轉型與阿拉伯芥轉殖.......................15 7、阿拉伯芥外表型與生理分析........................16 第三章 結果………………………………………………………………………..18 1、AtWRKY54與AtWRKY70在熱逆境下基因表現量的變化........18 2、AtWRKY54與AtWRKY70胺基酸序列同源性分析……………………….18 3、轉殖植株的鑑定與篩選……………………………………………………..18 4、熱休克轉錄因子與熱休克蛋白質的調控………………………………….19 5、阿拉伯芥受熱逆境的傷害評估…………………………………………….20 6、過氧化物(Reactive oxygen species, ROS)的累積與清除………………….21 7、阿拉伯芥耐熱性測試……………………………………………………….21 第四章 討論………………………………………………………………………..23 1、AtWRKY54與AtWRKY70對膜狀構造過氧化的影響...........23 2、AtWRKY54與AtWRKY70對膜狀構造穩定度的影響..........23 3、AtWRKY54與AtWRKY70對過氧化物累積的影響........24 4、抗氧化酵素活性的調控…………………………………………………….25 5、AtWRKY54與AtWRKY70對植物耐熱性的貢獻………………………….26 6、AtWRKY54與AtWRKY70下游作用機制探討…………………………….27 7、熱處理條件與葡萄糖濃度對於植物耐熱性實驗的影響.........27 第五章 參考文獻…………………………………………………………………..29 圖表…………………………………………………………………………………..40 圖表目錄 圖一 野生型阿拉伯芥AtWRKY54與AtWRKY70的表現量……………………....40 圖二 第三群WRKY轉錄因子胺基酸同源性分析………………………………..41 圖三 T-DNA insertion與Over-expression轉殖株鑑定與篩選……………………42 圖四 熱休克轉錄因子與熱休克蛋白質基因表現量分析…………………………43 圖五 Malondialdehyde (MDA)含量分析…………………………………………...44 圖六 細胞離子滲透率分析…………………………………………………………45 圖七 過氧化氫 (H2O2)累積量分析………………………………………………...46 圖八 GR與CAT酵素活性分析……………………………………………………47 圖九 下胚軸於熱逆境下的生長分析………………………………………………48 圖十 植株在熱逆境下的白化比率分析…………………………………………....49 表一 引子序列表……………………………………………………………………50 | |
dc.language.iso | zh-TW | |
dc.title | AtWRKY54 與 AtWRKY70 在熱逆境下的功能性分析 | zh_TW |
dc.title | Functional Characterization of AtWRKY54 and AtWRKY70 under Heat Stress | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 林讚標(Tsan-Piao Lin),張孟基(Men-Chi Chang),洪傳揚(Chwan-Yang Hong),常怡雍(Yee-Yung Charng) | |
dc.subject.keyword | AtWRKY54,AtWRKY70,丙二醛,過氧化氫,耐熱性, | zh_TW |
dc.subject.keyword | AtWRKY54,AtWRKY70,hydrogen peroxide,thermotolerance, | en |
dc.relation.page | 51 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-23 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 植物科學研究所 | zh_TW |
顯示於系所單位: | 植物科學研究所 |
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