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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 廖英志(Ying-Chih Liao) | |
dc.contributor.author | Yun-Ting Ma | en |
dc.contributor.author | 馬韻婷 | zh_TW |
dc.date.accessioned | 2021-06-16T23:46:38Z | - |
dc.date.available | 2015-07-27 | |
dc.date.copyright | 2012-07-27 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-23 | |
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Fan, L., et al., Preparation and properties of alginate/carboxymethyl chitosan blend fibers. Carbohydrate Polymers, 2006. 65(4): p. 447-452. 32. Roy, A., J. Bajpai, and A.K. Bajpai, Development of calcium alginate -gelatin based microspheres for controlled release of endosulfan as a model pesticide. Indian Journal of Chemical Technology, 2009. 16(5): p. 388-395. 33. Pelham, R.J. and Y.L. Wang, Cell locomotion and focal adhesions are regulated by substrate flexibility. Proceedings of the National Academy of Sciences of the United States of America, 1997. 94(25): p. 13661-13665. 34. Wang, H.-B., M. Dembo, and Y.-L. Wang, Substrate flexibility regulates growth and apoptosis of normal but not transformed cells. American Journal of Physiology - Cell Physiology, 2000. 279(5): p. C1345-C1350. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65497 | - |
dc.description.abstract | 在本研究過程中,為了達到兼具控制性及選擇性的細胞培養,我們提出了一個簡單且可靠的方法,即為使用噴墨技術製成所需之圖樣,再利用細胞本身所喜好生長環境因素的不同,以準確地控制細胞附著於生物可分解(biodegradable)材料上方。噴墨技術不僅可使材料成本降低,亦可以簡單地製作各式各樣不同的微圖形。
首先,藉由海藻酸鈉和氯化鈣產生交聯,並使用鑄造/溶劑蒸發法(casting / solvent evaporation method)製作出乾的方形基底薄膜,再利用控制液滴(drop-on-demand)的方法,在噴塗速度以及基板加熱溫度分別設定為20mm/s及30℃的情況下,可以在海藻酸鈣薄膜上噴塗各種不同的PLA圖案,例如線、圓形和方形等。本實驗中藉由噴塗不同的圖案提供細胞貼附,以證實可以控制3T3-L1細胞貼附在特定的位置。在實驗中細胞生長的概況分析,如細胞在基材上的貼附、貼附的數量以及細胞貼附的型態等,皆使用螢光顯微鏡與光學顯微鏡觀察。 在先前相關的研究中已經發現3T3細胞喜歡貼附在較硬的基材,故本實驗藉由噴墨技術噴塗一層到五層的PLA圖案,製作出因層數不同而具有硬度差異的PLA圖案,以探討硬度差異對3T3-L1細胞貼附的影響。硬度的計算是先利用AFM分析得到力與位移的曲線圖,再藉由Sneddon model做計算。當PLA層數越多,所得到的楊氏係數就越來越大,細胞貼附的量也就越多。最後,由實驗結果顯示,在細胞培養實驗中,因噴塗五層的PLA其硬度遠大於海藻酸鈣薄膜,可吸引較多的細胞貼附,所以本實驗可以利用基材硬度的不同,控制3T3-L1細胞貼附在特定的位置。 | zh_TW |
dc.description.abstract | In this research, we present an efficient and reliable approach to accurately control cell attachment positions on biodegradable substrates by inkjets printing technology. Sodium alginate cross-linked with calcium chloride is dried by casting /solvent evaporation method to prepare smooth square films. A drop-on-demand method is used to print various patterns of biodegradable material (PLA), such as circles, squares, and lines, which is a specific marker for 3T3-L1 cells. Cell growth profile, such as cell attachment, density and morphology, on various patterns are examined and studied by confocal fluorescence microscopy and optical microscopy. Smooth PLA pattern on calcium alginate film can be obtained when velocity of moving stage was set to 20
mm/s and the substrate temperature was kept at 30℃. Previous studies showed that 3T3 cells prefer to attach to harder substrates. By this principle, to investigate effects of rigidity variation on cell attachment, multiple layers of PLA were printed on alginate to generate composite substrates with different PLA layer. The Young’s moduli of printed PLA layers on alginate are determined with AFM. Through AFM indentation, force–distance curves can be used to calculate Young’s moduli of composite films from Sneddon model. In the result, significantly more cells were attached to PLA with more printed PLA layers, or composite films with higher Young’s modulus. This rigidity-guided attachment approach can be used to create cell culture on various patterns. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:46:38Z (GMT). No. of bitstreams: 1 ntu-101-R99524041-1.pdf: 6996929 bytes, checksum: 1428bc98124b91b39188ee40e2f037cb (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 中文摘要…………………………………………………………………………… I
英文摘要……………………………………………………………………… II 誌謝…………………………………………………………………………………… III 目錄…………………………………………………………………………………… IV 圖目錄………………………………………………………………………………… VI 表目錄………………………………………………………………………………... X 第一章 緒論………………………………………………………………………. 1 1.1 前言………………………………………………………………………. 1 1.2 文獻回顧…………………………………………………………………… 2 1.2.1 生醫材料…………………………………………………………… 2 1.2.2 貼附型細胞貼附機制……………………………………………… 6 1.2.3 使用噴墨技術製備微圖案控制細胞貼附………………………… 7 1.2.4 基材硬度與表面粗糙度對細胞的影響…………………………… 13 1.3 論文架構…………………………………………………………………… 18 第二章 噴墨系統介紹……………………………………………………………… 21 A. 噴墨模組……………………………………………………………… 21 B. 觀測設備和移動平台………………………………………………… 22 C. 軟體操作 ……………………………………………………………… 22 第三章 實驗物品與流程…………………………………………………………… 27 3.1 實驗藥品與儀器………………………………………………………… 27 3.1.1實驗藥品…………………………………………………………… 27 3.1.2實驗儀器…………………………………………………………… 28 3.2 實驗流程…………………………………………………………………… 29 3.2.1海藻酸鈣 (calcium alginate)薄膜之製作………………………...… 29 3.2.2 PLA墨水配製…………………………………..……………….…… 31 3.2.3 MicroFab jetlab 4操作……….……………………………………… 32 3.2.4 細胞培養及染色………………………………………..…………… 34 A. 細胞培養……………………………………………………… 34 B. 細胞染色……………………………………………………… 36 3.3分析儀器…………………………………………………………………… 38 3.3.1原子力顯微鏡(Atomic Force Microscopy, AFM)……………… 38 3.3.2掃描式電子顯微鏡(Scanning Electron Microscope, SEM)…… 42 3.3.3水樣分析儀………………………………………………………… 43 第四章 利用噴墨技術噴塗聚乳酸(PLA)墨水………………….………………… 45 4.1 噴墨液滴生成與控制……………………………...……………………… 45 4.2 墨水的配方對於PLA在基材表面成膜性的影響………………………… 50 4.3 不同噴塗情況下對於圖案(Pattern)呈現的影響…………………………… 52 4.4 利用噴墨技術控制聚乳酸(PLA)墨水於基材上之形成…………………… 56 4.5 使用噴墨技術控制微圖案在海藻酸鈣薄膜上方之形成………..………… 60 第五章 PLA的固定與PLA的層數對細胞貼附之影響…...……………………… 63 5.1海藻酸鈣薄膜的膨潤率與含水率分析…………………………………... 63 5.2 PLA固定於海藻酸鈣薄膜之研究………………………………………… 66 5.3 PLA軟硬度對細胞貼附的影響…………………………………………… 69 5.4 PLA表面粗糙度對細胞貼附的影響……………………………………… 76 5.5控制細胞貼附於特定的位置……………………………………………… 81 第六章 結論………………………………………………………………………… 89 第七章 未來展望…………………………………………………………………… 91 參考文獻……………………………………………………………………………… 93 作者簡述……………………………………………………………………………… 97 | |
dc.language.iso | zh-TW | |
dc.title | 利用噴墨技術將生物可分解之材料製成圖形來控制細胞培養 | zh_TW |
dc.title | Patterning Cell Cultures on Biodegradable Substrates by Inkjet Technology | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 徐振哲(Cheng-Che Hsu),游佳欣(Jiashing Yu),蕭浩明,盧彥文 | |
dc.subject.keyword | 噴墨技術,海藻酸鈉,聚乳酸,生物可分解材料,3T3-L1細胞, | zh_TW |
dc.subject.keyword | Inkjets printing technology,Sodium alginate,poly lactic acid (PLA),Biodegradable material,3T3-L1 cells, | en |
dc.relation.page | 97 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-24 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 化學工程學研究所 | zh_TW |
顯示於系所單位: | 化學工程學系 |
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