請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65343完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳億乘(Chen-Yi Chen) | |
| dc.contributor.author | Yi-Tsen Lin | en |
| dc.contributor.author | 林宜岑 | zh_TW |
| dc.date.accessioned | 2021-06-16T23:37:35Z | - |
| dc.date.available | 2017-08-28 | |
| dc.date.copyright | 2012-08-28 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-07-25 | |
| dc.identifier.citation | 張勝善。1989。牛乳與乳製品。長河出版社。台北。台灣。
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65343 | - |
| dc.description.abstract | 隨著畜產品之開發越來越多元化，如能針對雞肝之特性加以開發利用，則可增加相關產業之收益，亦可提高其附加價值。欲開發雞肝之利用價值，本試驗中利用酵素水解，將雞肝酵素水解為雞肝水解物(chicken-liver hydrolysates, CLH)，進行成分分析、體外抗氧化能力與體內抗氧化功效之評估。體外試驗結果中發現：由alcalase、papain與pepsin水解雞肝試驗中，以pepsin水解產物之DPPH自由基清除能力最佳，因而選擇pepsin作為後續水解雞肝之用；接著藉由製成率、蛋白質與胜肽含量變化、成本效益、及體外抗氧化試驗，篩選出酵素受質比1:400水解2小時，作為水解雞肝之最適條件。CLH之成分變化分析結果中發現，雞肝經酵素水解後，胜肽片段皆小於10 KDa，而胺基酸組成中以天門冬胺酸與麩胺酸此兩種酸性胺基酸含量最高；而CLH中含有抗氧化金屬離子成分錳、硒，而有害重金屬離子，鎘、汞、錫、砷等含量皆遠遠低於國家標準。
接續評估CLH之體內抗氧化功效，實驗中將利用D-半乳糖誘導體內氧化壓力上升之C57BL/6小鼠作為此次試驗之動物模型；確立D-半乳糖動物模型試驗中，選定以1.2 g D-半乳糖/ kg BW作為最適誘導劑量。體內抗氧化功效評估試驗中，D-半乳糖處理之小鼠同時補充低劑量(50 mg/kg BW)與高劑量(250 mg/kg BW)之CLH，經由分析小鼠體內氧化壓力與抗氧化酵素活性結果中發現，D-半乳糖處理組之體內氧化壓力顯著高於控制組(P<0.05)，而體內抗氧化酵素活性也顯著低於控制組(P<0.05)；補充CLH之組別，可顯著改善D-半乳糖誘導下氧化壓力上升與抗氧化酵素活性下降之現象(P<0.05)。綜觀上述可知，CLH具有良好之體外與體內抗氧化能力。 | zh_TW |
| dc.description.abstract | To enhance the value of chicken livers, this study was to develop antioxidative chicken-liver hydrolysates (CLHs) via an enzymatic hydrolysis. According to the in vitro results, the CLHs produced by pepsin showed the best (P<0.05) DPPH scavenging ability than those produced by alcalase and papain. Furthermore, the optimal hydrolyzing conditions were chosen as the enzyme to substrate ratio, 1:400 and 2hours based on yield, protein and peptide contents, costs, and in vitro antioxidative tests of CLHs. An SDS-PAGE analysis indicated that molecular weights of peptides in final manufactured CLHs were lower than 10KDa. Besides, CLHs were rich of acidic amino acids, i.e. aspartic acid (Asp) and glutamic acid (Glu), and also contained both manganese (Mn) and selenium (Se) which are essential cofactors of antioxidative enzymes, superoxide dismutase and glutathione peroxidase, respectively. The contents of heavy metal ions. i.e. cadmium (Cd), mercury (Hg), tin (Sn) and arsenic (As) in CLHs did not exceed the regulatory levels of Taiwan Food and Drug Administration (TFDA) as well.
To evaluate the in vivo antioxidative effects of CLHs, the oxidative animal model was successfully established by 1.2g D-galactose (DG)/kg BW in male 12-week-old C57BL/6 mice. After 6 weeks of experimental period, DG increased (P<0.05) TBARS values and decreased (P<0.05) GSH and TEAC levels in serum and organs compared to non-DG treatments. However, those antioxidative values were improved (P<0.05) by CLH cotreatments. In conclusion, our manufactured CLHs showed antioxidative abilities which may result from its peptide structures, acidic amino acids, and antioxidative minerals. We hope that this study not only diversifies animal byproducts to increase the byproduct’s value but also offers consumer another choice of healthy ingredients from animals. | en |
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| dc.description.tableofcontents | 中文摘要………………………………………………………………………… 1
英文摘要………………………………………………………………………… 2 前言…………………………………………………………………………………… 3 壹、文獻檢討……………………………………………………………………. 5 一、雞肝之簡介………………………………………………………………… 5 二、蛋白質水解胜肽及相關抗氧化功能介紹………………………………… 10 （一）蛋白質水解方法………………………………………………………… 10 （二）蛋白質酵素水解物之優點……………………………………………… 11 （三）蛋白質酵素水解物之缺點與改善……………………………………… 11 （四）具有抗氧化功效之蛋白質水解胜肽…………………………………… 12 三、自由基之產生及體內抗氧化防禦系統…………………………………….. 14 （一）自由基與活性氧………………………………………………………… 14 （二）自由基與活性氧引起之傷害…………………………………………… 17 （三）體內抗氧化防禦系統…………………………………………………… 20 （四）常見之抗氧化物質……………………………………………………… 23 四、Ｄ－半乳糖誘導體內氧化壓力上升之機制與相關動物試驗…………… 31 （一）Ｄ－半乳糖誘導體內氧化壓力上升之機制…………………………… 31 （二）Ｄ－半乳糖誘導之相關疾病…………………………………………… 32 （三）衛生署食品藥物管理局對於健康食品之延緩衰老功能評估方法…… 33 貳、材料與方法………………………………………………………………….. 34 一、實驗流程…………………………………………………………………… 34 二、實驗材料…………………………………………………………………… 35 三、水解物製備………………………………………………………………… 38 （一）水解物酵素之決定……………………………………………………… 38 （二）水解物製備條件之確立………………………………………………… 39 四、酵素水解物之分析項目…………………………………………………... 42 （一）水解過程成分變化分析………………………………………………… 42 （二）抗氧化分析項目與方法………………………………………………… 44 五、動物試驗…………………………………………………………………….. 47 （一）確立Ｄ－半乳糖誘導氧化壓力上升之動物模型……………………… 47 （二）雞肝酵素水解物減緩Ｄ－半乳糖誘導之氧化壓力上升動物試驗…... 47 （三）小鼠之生理表現評估…………………………………………………… 49 （四）小鼠體內氧化壓力之評估……………………………………………… 50 （五）小鼠體內抗氧化酵素活性分析………………………………………… 53 六、統計………………………………………………………………………… 54 叁、結果與討論.................................................. 55 一、雞肝水解酵素之決定……………………………………………………… 55 二、雞肝水解物製備條件確立………………………………………………… 60 三、雞肝水解物成分變化分析………………………………………………… 74 四、確立Ｄ－半乳糖誘導小鼠氧化壓力上升之動物模型…………………… 84 （一）小鼠體內氧化壓力之評估……………………………………………… 84 （二）小鼠體內抗氧化酵素活性評估………………………………………… 85 五、雞肝水解物減緩Ｄ－半乳糖誘導小鼠氧化壓力上升試驗……………… 90 （一）小鼠生理表現評估……………………………………………………… 90 （二）小鼠體內氧化壓力之評估……………………………………………… 97 （三）小鼠體內抗氧化酵素活性評估………………………………………… 103 肆、結論………………………………………………………………………… 107 參考文獻………………………………………………………………………… 109 | |
| dc.language.iso | zh-TW | |
| dc.subject | 抗氧化 | zh_TW |
| dc.subject | 雞肝 | zh_TW |
| dc.subject | 半乳糖 | zh_TW |
| dc.subject | chicken-liver | en |
| dc.subject | antioxidative | en |
| dc.subject | galactose | en |
| dc.title | 雞肝水解物之製備與抗氧化功效評估 | zh_TW |
| dc.title | studies on manufacture and antioxidative effects of chicken-liver hydrolysates | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 許桂森(Kuei-Sen Hsu),邱智賢(Chih-Hsien Chiu),周崇熙(Chung-Hsi Chou),劉?睿(Che-Jui Liu) | |
| dc.subject.keyword | 雞肝,抗氧化,半乳糖, | zh_TW |
| dc.subject.keyword | chicken-liver,antioxidative,galactose, | en |
| dc.relation.page | 118 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-07-26 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 動物科學技術學研究所 | zh_TW |
| 顯示於系所單位： | 動物科學技術學系 | |
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| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-101-1.pdf 未授權公開取用 | 3.26 MB | Adobe PDF |
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