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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 楊申語(Sen-Yeu Yang) | |
dc.contributor.author | MING-TUNG Lin | en |
dc.contributor.author | 林銘棟 | zh_TW |
dc.date.accessioned | 2021-06-16T23:32:00Z | - |
dc.date.available | 2022-12-31 | |
dc.date.copyright | 2012-08-01 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-07-29 | |
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/65239 | - |
dc.description.abstract | 多孔性3D生醫支架主要運用於組織工程中,利用自體之組織細胞進行體外培養,再經由生長因子或訊號的刺激,使細胞生長分化成所需之組織形貌,待組織生長後植回體內,達到組織修復之目的。
現今製作支架的方式,大多採用有機溶劑或快速成型等方式製作,前者有溶劑揮發不完全問題;後者有無法一體成型,需使用電腦複雜程序控制一層一層堆積成所需孔洞支架。本研究開發氣壓轉注加壓成型機,特點為不使用任何有機溶劑,即可製作3D具孔洞支架,且可一體成形,可快速製作支架。本研究在製作圓盤型生醫支架前,先行進行最佳混合比測試,擇出以PLGA與NaCl重量比1:11有最佳支架性質。接著,進行尋求最佳成型參數與探討其支架機械性質,實驗結果其最佳成形參數為模溫90℃、料管加熱溫度200℃,壓力為4 kgf/cm2,製作出支架其孔隙率90%以上,孔洞大小為200~500 μm,符合其生醫用軟骨細胞生長大小。另外,本研究也進行縮小版人耳形狀支架製作,以材質PDMS作為軟模進行測試,實驗證實可製作出具3D孔洞耳朵型支架,孔洞大小200~500 μm。 圓盤型支架製作後,進行支架降解性測試,及實際細胞生長於支架上,結果顯示,於定性檢測中,使用SEM及螢光共焦顯微儀觀察細胞生長情形,皆有明顯貼覆及生長於支架內部;於定量檢測中,細胞基因表現、MTT活性檢測,皆顯示隨培養天數增加,細胞於支架適應性提高,細胞生長量與細胞外基質含量也隨之增加,MTT試劑檢測於一天時,有84%之軟骨細胞生長率,顯示軟骨細胞對於此支架環境適應良好。 | zh_TW |
dc.description.abstract | Porous 3D biomedical scaffolds have been widely used in tissue engineering. The scaffold can be used to culture autologous cells in vivo, stimulated by growth factors and signals. The cells can grow into the desired tissue morphology. Finally, it can be transplanted back into the body, through the stable tissue growth achieving the purpose of tissue repair. Conventional scaffolds are mostly prepared by organic solvents or rapid prototyping. There are problems such as residual solvent and complicated process. In order to solve these problems, a pneumatic transfer molding machine is designed and implemented to fabricate scaffolds in this study.
We first mold the disk biomedical scaffolds. It is found that the best weight ratio of PLGA:NaCl is 1:11. The experimental result also shows the best forming parameters are 90℃ for mold temperature, 200℃for the tube heating temperature and 4 kgf/cm2 for gas pressure. The scaffolds after particulate leaching yield porosity over 90% with pore size 200 ~ 500 μm, which satisfies what Health Medical chondrocytes need. Furthermore, the narrow version of the human-ear-shaped scaffold is also molded in this research. The mold is fabricated by rapid protyping and then by casting using PDMS. The transfer moldings of PLGA/NaCl using PDMS mold, after leaching, can yield ear-shaped scaffolds with pore size 200 ~ 500 μm. The disk scaffold is used to culture cartilage cells. The morphology and distribution of the chondrocytes have been examined by fluorescence confocal microscopy and SEM. The cell growth behaviors has been determined by MTT assay, collagen, and glycosaminoglycan (GAG) secretion. After culture for 1 day, 84% of cartilage cells attached on scaffold. This proves that the transfer molded PLGA/NaCl, after leaching, can yield scaffolds with interconnected pores for culturing cells. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:32:00Z (GMT). No. of bitstreams: 1 ntu-101-R99522707-1.pdf: 11797152 bytes, checksum: c5da5501b951f47986a3a51df80a4e21 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 致謝 I
摘要 II Abstract III 目錄 IV 圖目錄 VII 表目錄 XII 第一章 導論 1 1.1前言 1 1.2組織工程 2 1.2.1細胞 4 1.2.2生醫用降解高分子材料 4 1.2.3支架製作原則 6 1.3常見軟骨修復與重建方法 7 1.4結構元件複製成型技術 11 1.5研究動機與目的 16 1.6論文架構 16 第二章 文獻回顧 18 2.1生醫支架製作文獻回顧 18 2.2微射出成型相關文獻 27 2.3變模溫系統 31 2.4軟模用於結構複製文獻 32 2.5文獻總結與研究創新 36 第三章 實驗設備與實驗方法 38 3.1實驗流程圖 38 3.2實驗概述 38 3.3實驗設計 40 3.3.1氣壓轉注成型機之組裝 40 3.3.2模具製作(為圓盤與耳朵形狀製作) 48 3.4實驗材料與設備操作流程 59 3.4.1材料與物性 59 3.4.2實驗設備與操作流程 62 3.4.3孔洞支架性質相關量測機台 67 3.5實驗方法規劃 72 3.5.1製備生醫孔洞支架 72 3.5.2孔洞支架性質探討 74 3.5.3軟骨細胞取得/植入支架培養 76 3.5.4軟骨細胞植入後探討 77 第四章 氣壓轉注成型應用於生醫支架之成型參數探討 83 4.1生醫材料分析 83 4.1.1熱重量分析儀TGA分析 83 4.1.2差分掃描量熱儀DSC分析 85 4.2氣壓轉注成型步驟及混合均勻度測試 86 4.2.1氣壓轉注成型步驟 86 4.2.2混合均勻度之測試 87 4.3不同混合比例測試 90 4.3.1不同混合比之測試 90 4.3.2支架性質量測與分析 91 4.4成型參數規劃與結果 96 4.4.1成型參數規劃 96 4.4.2成型結果與支架性質量測分析 97 4.5結論 104 第五章 圓盤型支架力學與降解特性測試及耳朵型支架之製作 106 5.1不同混合比例下之支架壓縮試驗 106 5.2 PDMS耳朵型支架之製作 109 5.3隨時間不同比例支架降解測試 112 5.4結論 114 第六章 PLGA圓盤型支架應用於細胞培養 115 6.1軟骨細胞之來源與接種至支架 115 6.2有/無細胞支架之力學測試結果 119 6.3含軟骨細胞支架之定量測試結果 122 6.3.1細胞貼覆與細胞活性MTT assay之測試結果 122 6.3.2細胞與細胞外基質之含量測試結果 125 6.4含軟骨細胞支架之定性測試結果 128 6.4.1 Live&Dead/SEM之測試結果 128 6.4.2細胞基因表現之測試結果 137 6.4.3細胞組織學染色之測試結果 140 6.5 結論 144 第七章 結論與未來展望 146 7.1 研究成果總結 146 7.1.1不同混合比例探討 146 7.1.2成型參數探討 147 7.1.3支架機械性質探討 147 7.1.4 PDMS耳朵型軟模開發與支架製作 147 7.1.5支架降解測試 148 7.1.6細胞培養性質測試 148 7.2 未來研究方向與展望 149 參考文獻 150 | |
dc.language.iso | zh-TW | |
dc.title | 氣壓轉注成型製作三維生醫支架於組織工程之應用 | zh_TW |
dc.title | Pneumatic Transfer Molding to Fabricate Three-Dimensional Biodegradable Porous Scaffolds | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 李明義,劉士榮,沈永康,陳志豪 | |
dc.subject.keyword | 氣壓轉注加壓成型,孔隙率,吸水率,生醫支架,PDMS,細胞,軟骨, | zh_TW |
dc.subject.keyword | Pneumatic Transfer Molding,Porosity,Mositure,Scaffold,PDMS,Cell,Cartilage, | en |
dc.relation.page | 157 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-07-30 | |
dc.contributor.author-college | 工學院 | zh_TW |
dc.contributor.author-dept | 機械工程學研究所 | zh_TW |
顯示於系所單位: | 機械工程學系 |
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