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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃升龍(Sheng-Lung Huang) | |
dc.contributor.author | Tsung-Hsun Hsieh | en |
dc.contributor.author | 謝宗勳 | zh_TW |
dc.date.accessioned | 2021-06-16T23:14:03Z | - |
dc.date.available | 2017-08-15 | |
dc.date.copyright | 2012-08-15 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-01 | |
dc.identifier.citation | [1] Huang, D., Swanson, E.A., Lin, C.P., et al., Optical coherence tomography. Science, 1991. 254(5035): p. 1178-1181.
[2] Zysk, A.M., Nguyen, F.T., Oldenburg, A.L., et al., Optical coherence tomography: a review of clinical development from bench to bedside. J Biomed Opt, 2007. 12(5): p. 051403. [3] Mogensen, M., Thrane, L., Jørgensen, T.M., et al. Optical coherence tomography for imaging of skin and skin diseases. 2009. Elsevier. [4] Strasswimmer, J., Pierce, M.C., Park, B.H., et al., Polarization-sensitive optical coherence tomography of invasive basal cell carcinoma. J Biomed Opt, 2004. 9(2): p. 292-298. [5] Olmedo, J.M., Warschaw, K.E., Schmitt, J.M., et al., Optical coherence tomography for the characterization of basal cell carcinoma in vivo: a pilot study. J Am Acad Dermatol, 2006. 55(3): p. 408-412. [6] Di Carlo, D. and Lee, L.P., Dynamic single-cell analysis for quantitative biology. Anal Chem, 2006. 78(23): p. 7918-7925. [7] Larabell, C.A. and Le Gros, M.A., X-ray tomography generates 3-D reconstructions of the yeast, saccharomyces cerevisiae, at 60-nm resolution. Mol Biol Cell, 2004. 15(3): p. 957-962. [8] Ting, C.S., Hsieh, C., Sundararaman, S., et al., Cryo-electron tomography reveals the comparative three-dimensional architecture of Prochlorococcus, a globally important marine cyanobacterium. J Bacteriol, 2007. 189(12): p. 4485-4493. [9] Nandakumar, V., Kelbauskas, L., Johnson, R., et al., Quantitative characterization of preneoplastic progression using single-cell computed tomography and three-dimensional karyometry. Cytometry A, 2011. 79(1): p. 25-34. [10] Miao, Q., Yu, J., Rahn, J.R., et al., Dual-mode optical projection tomography microscope using gold nanorods and hematoxylin-stained cancer cells. Opt Lett, 2010. 35(7): p. 1037-1039. [11] Tan, W., Vinegoni, C., Norman, J.J., et al., Imaging cellular responses to mechanical stimuli within three-dimensional tissue constructs. Microsc Res Tech, 2007. 70(4): p. 361-371. [12] Choi, W., Fang-Yen, C., Badizadegan, K., et al., Tomographic phase microscopy. Nat Methods, 2007. 4(9): p. 717-719. [13] Ciobanu, L. and Pennington, C.H., 3D micron-scale MRI of single biological cells. Solid State Nucl Magn Reson, 2004. 25(1-3): p. 138-141. [14] Wojtkowski, M., High-speed optical coherence tomography: basics and applications. Appl Opt, 2010. 49(16): p. D30-61. [15] Drexler, W. and Fujimoto, J.G., Optical Coherence Tomography Technology and Applications Optical Coherence Tomography: Technology and Applications, 2008: p. V-Vii. [16] Boulon, G., Laversenne, L., Goutaudier, C., et al., Radiative and non-radiative energy transfers in Yb3+-doped sesquioxide and garnet laser crystals from a combinatorial approach based on gradient concentration fibers. J Lumin, 2003. 102: p. 417-425. [17] Burrus, C. and Stone, J., Single-crystal fiber optical devices: A Nd: YAG fiber laser. Appl Phys Lett, 1975. 26(6): p. 318-320. [18] Dong, Y.J., Zhou, G.Q., Xu, J., et al., Luminescence studies of Ce : YAG using vacuum ultraviolet synchrotron radiation. Mater Res Bull, 2006. 41(10): p. 1959-1963. [19] 鄭乃嘉, 結合光學同調斷層掃描與共焦螢光顯微術之研究. 國立臺灣大學光電工程學研究所, 2010. [20] http://barefacedtruth.com/skin-101/ [21] http://en.wikipedia.org/wiki/File:Skinlayers.png [22] Schaeffer, W.I., Terminology associated with cell, tissue, and organ culture, molecular biology, and molecular genetics. Tissue Culture Association Terminology Committee. In Vitro Cell Dev Biol, 1990. 26(1): p. 97-101. [23] Freshney, R.I., Culture of animal cells: a manual of basic technique and specialized applications2010: Wiley-Blackwell. [24] Hayflick, L. and Moorhead, P.S., The serial cultivation of human diploid cell strains. Experimental cell research, 2006. 25(3): p. 585-621. [25] Rubin, A.I., Chen, E.H. and Ratner, D., Basal-cell carcinoma. N Engl J Med, 2005. 353(21): p. 2262-2269. [26] Chiang, L.C., Chiang, W., Yu, H.S., et al., Establishment and characterization of a continuous human basal cell carcinoma cell line from facial skin (I) cytological behavior of early passages. The Kaohsiung journal of medical sciences, 1994. 10(4): p. 170-176. [27] Boukamp, P., Petrussevska, R.T., Breitkreutz, D., et al., Normal keratinization in a spontaneously immortalized aneuploid human keratinocyte cell line. J Cell Biol, 1988. 106(3): p. 761-771. [28] http://www.bcrc.firdi.org.tw/BSAS_cart/controller?event=SEARCH&bcrc_no=6 0038 [29] Kerr, J.F., Wyllie, A.H. and Currie, A.R., Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics. Br J Cancer, 1972. 26(4): p. 239-257. [30] http://www.celldeath.de/encyclo/aporev/aporev.htm [31] Vaux, D.L. and Korsmeyer, S.J., Cell death in development. Cell, 1999. 96(2): p. 245-254. [32] Saraste, A. and Pulkki, K., Morphologic and biochemical hallmarks of apoptosis. Cardiovasc Res, 2000. 45(3): p. 528-537. [33] Meier, P., Finch, A. and Evan, G., Apoptosis in development. Nature, 2000. 407(6805): p. 796-801. [34] Elmore, S., Apoptosis: a review of programmed cell death. Toxicol Pathol, 2007. 35(4): p. 495-516. [35] Van Cruchten, S. and Van Den Broeck, W., Morphological and biochemical aspects of apoptosis, oncosis and necrosis. Anat Histol Embryol, 2002. 31(4): p. 214-223. [36] Hughes, C.S., Postovit, L.M. and Lajoie, G.A., Matrigel: a complex protein mixture required for optimal growth of cell culture. Proteomics, 2010. 10(9): p. 1886-1890. [37] Kleinman, H.K. and Martin, G.R., Matrigel: basement membrane matrix with biological activity. Semin Cancer Biol, 2005. 15(5): p. 378-386. [38] Csiki, I., Morrow, J.D., Sandler, A., et al., Targeting cyclooxygenase-2 in recurrent non-small cell lung cancer: a phase II trial of celecoxib and docetaxel. Clin Cancer Res, 2005. 11(18): p. 6634-6640. [39] Reckamp, K.L., Krysan, K., Morrow, J.D., et al., A phase I trial to determine the optimal biological dose of celecoxib when combined with erlotinib in advanced non-small cell lung cancer. Clin Cancer Res, 2006. 12(11 Pt 1): p. 3381-3388. [40] Mourant, J.R., Freyer, J.P., Hielscher, A.H., et al., Mechanisms of light scattering from biological cells relevant to noninvasive optical-tissue diagnostics. Appl Opt, 1998. 37(16): p. 3586-3593. [41] Drezek, R., Dunn, A. and Richards-Kortum, R., Light scattering from cells: finite-difference time-domain simulations and goniometric measurements. Appl Opt, 1999. 38(16): p. 3651-3661. [42] Darzynkiewicz, Z., Juan, G., Li, X., et al., Cytometry in cell necrobiology: analysis of apoptosis and accidental cell death (necrosis). Cytometry, 1997. 27(1): p. 1-20. [43] Darzynkiewicz, Z., Bedner, E. and Smolewski, P., Flow cytometry in analysis of cell cycle and apoptosis. Semin Hematol, 2001. 38(2): p. 179-193. [44] Drezek, R., Guillaud, M., Collier, T., et al., Light scattering from cervical cells throughout neoplastic progression: influence of nuclear morphology, DNA content, and chromatin texture. J Biomed Opt, 2003. 8(1): p. 7-16. [45] http://en.wikipedia.org/wiki/K-means_clustering | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64997 | - |
dc.description.abstract | 超高解析光學同調斷層掃描(ultrahigh-resolution optical coherence tomography; UR-OCT)首次應用於體外單顆皮膚細胞的三維造影與分析研究上。此非侵入式、毋須標定、以及擁有極深成像與細胞等級之空間解析能力的光學偵測技術,可從三維的方式來分析單顆細胞之散射特性。基於UR-OCT影像以及細胞形態學上的觀察,我們可以很容易地辨別活的和細胞凋亡之基底癌細胞。除此之外,我們首次提出一種全新且快速之自動提取個別單顆皮膚細胞之特徵參數的方法,包含訊號平均、細胞體積、細胞密度及平均動態範圍。並藉此分析方法進行了定量比較和參數分析,其中三個特徵參數p-value小於0.05。實驗結果顯示出UR-OCT於細胞級別上具有檢測細胞死亡與否之能力。
更進一步,我們藉由圖像分割中屬於型態學影像處理之膨脹與侵蝕技術,進行一系列的影像處理,並計算出由UR-OCT拍攝的HaCaT之細胞大小和細胞核體積,進而推算出細胞核質比為0.18,非常接近螢光顯微鏡之結果。 最後,我們採用HaCaT與Hs68細胞株作為表皮中的角質細胞與真皮中的纖維母細胞代表,同樣使用自行定義之特徵參數,藉由圖像分割中的k-means聚類法,從統計學上對不同類型的皮膚細胞進行區分。我們展現了總共12顆的HaCaT與Hs68細胞可以很準確且快速地被辨別,暗示了UR-OCT於未來皮膚科臨床應用與組織工程上扮演一個重要角色。 | zh_TW |
dc.description.abstract | Ultrahigh-resolution optical coherence tomography (UR-OCT) has been used to study single skin cells in vitro for the first time. This non-invasive and label-free optical detection technique with deep imaging depth can be used to analyze scattering properties of single cells in three-dimensional with cellular spatial resolution. Based on morphological observation of the UR-OCT images, live and apoptotic basal cell carcinoma (BCC) can be easily identified. In addition, we developed a novel method to automatically extract the characteristic parameters which defined as signal average, cell volume, cellular density and average dynamic range of individual cells. Quantitative comparison showed that three parameters were found with p-value smaller than 0.05. The experimental results show that the ability of UR-OCT to detect cell death at cellular level.
To find the nuclear-cytoplasmic ratio (N:C ratio) of single cells, we did a series of image segmentation processes by means of morphological dilation and erosion. In this way, calculated N:C ratio of single HaCaT cell was 0.18, which was very close to the observation of confocal fluorescence microscopy. Finally, the HaCaT and Hs68 cell lines were used to model epidermis and dermis respectively. We applied clustering analysis on these cells by k-means clustering method based on the characteristic parameters. We demonstrate that a total of 12 HaCaT and Hs68 cells can be distinguished very quickly and accurately, indicating that the UR-OCT will play an important role in clinical dermatology and tissue engineering in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T23:14:03Z (GMT). No. of bitstreams: 1 ntu-101-R99941069-1.pdf: 5520993 bytes, checksum: 0cf98d496d649d81f3442098f821ce1b (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 摘要 I
Abstract II 目錄 III 圖目錄 V 表目錄 VII 第一章 緒論 1 第二章 實驗系統理論與架構 4 2.1 光學同調斷層掃描 4 2.1.1 低同調干涉術 4 2.1.2 時域光學同調斷層掃描 6 2.1.3 系統解析度 7 2.2 光學系統架構 9 2.2.1 系統光源 9 2.2.2 實驗系統架構 14 第三章 細胞凋亡之辨別與分析 17 3.1 皮膚細胞 17 3.1.1 細胞株與初生細胞 19 3.1.2 基底癌細胞 20 3.1.3 HaCaT細胞株 20 3.1.4 Hs68 細胞株 21 3.2 細胞凋亡 23 3.2.1 細胞凋亡之型態特徵 24 3.2.2 樣本製備 28 3.3 實驗結果 29 3.3.1 單顆基底癌細胞之三維造影 29 3.3.2 基底癌細胞之UR-OCT、CFM與CRM之比對 32 3.3.3 特性參數定義 33 3.3.4 分析與討論 35 第四章 正常皮膚細胞之分析 40 4.1 圖像分割 40 4.2 細胞核質比 43 4.3 表皮細胞與真皮細胞之辨別 46 第五章 結論與未來展望 50 參考文獻 52 | |
dc.language.iso | zh-TW | |
dc.title | 超高解析光學同調斷層掃描於單顆皮膚細胞之造影與分析 | zh_TW |
dc.title | Imaging and Analysis of Single Skin Cell by
Ultrahigh-Resolution Optical Coherence Tomography | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃鼎偉(Ding-Wei Huang),宋孔彬(Kung-Bin Sung),葉秉慧 | |
dc.subject.keyword | 光學同調斷層掃描,單顆細胞,基底癌細胞,細胞凋亡, | zh_TW |
dc.subject.keyword | Optical coherence tomography,Single cell,Basal cell carcinoma,Apoptosis, | en |
dc.relation.page | 57 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-03 | |
dc.contributor.author-college | 電機資訊學院 | zh_TW |
dc.contributor.author-dept | 光電工程學研究所 | zh_TW |
顯示於系所單位: | 光電工程學研究所 |
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