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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 林萬寅(Wann-Yin Lin) | |
| dc.contributor.author | Chia-Ming Kuo | en |
| dc.contributor.author | 郭家銘 | zh_TW |
| dc.date.accessioned | 2021-05-17T09:14:06Z | - |
| dc.date.available | 2015-08-22 | |
| dc.date.available | 2021-05-17T09:14:06Z | - |
| dc.date.copyright | 2012-08-22 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-16 | |
| dc.identifier.citation | [1] Kohlarush, F., Wiedemanns Ann. 1897, 62, 209.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6480 | - |
| dc.description.abstract | 本論文選擇以類黃酮化合物(flavonoids)以毛細管電泳法來探討偵測靈敏度的增強，注重小分子在毛細管電泳上之線上濃縮技巧。結果分為兩大部分：
第一部份先以毛細管區帶電泳法(CZE)及微胞電動力層析法(MEKC)做分離，接著測試幾種不同堆積模式，包括電極極性反向堆積模式(large volume sample stacking with reverse electrode polarity, LVSS)及大體積反向電壓微胞掃集模式(large volume sample stacking-sweeping, LVSS-sweeping)或正向添加SDS的掃集模式，其中LVSS-Sweeping及正向掃集的最佳化條件較難求得，而LVSS法於0.4 分鐘時切換電壓方向可得良好的堆積，並測得hesperetin、naringenin、quercetin與kaempferol的偵測極限分別14.87、14.71、19.76與16.66 ng/mL(S/N = 3)，並可用於真實樣品偵測。 第二部份強調於逆向掃集模式的改良，最佳條件為使用掃集模式(sweeping)，於分離緩衝中及樣品基質中添加適當電解質，有助改善長時間進樣的樣品濃縮。甚至可讓注入類黃酮化合物時間達480秒，最大樣品體積約佔毛細管總長的96.5 %。樣品基質使用pH 2.0的20 mM磷酸鹽緩衝液，分離緩衝液為pH 2.0的20 mM樣品注入時間為120秒時，磷酸緩衝液並分別添加50 mM的SDS，ACN則分別添加10 %。而120秒的樣品注入所得hesperetin、naringenin、quercetin及kaempferol之偵測極限分別為43.53、38.25、57.38及48.55 ng/mL(S/N = 3)。而480秒的樣品注入所得hesperetin、naringenin、quercetin及kaempferol之偵測極限分別為21.28、15.37、24.26及24.7 ng/mL(S/N = 3)。並能有效應用於真實樣品之檢測。 | zh_TW |
| dc.description.abstract | In this dissertation, four flavonoid analytes were selected to investigate the enhancement of detection sensitivity by capillary electrophoresis. Several on-line concentration modes studied were divided into two parts:
In the first part, CZE and MEKC modes were studied first, and then the large volume stacking with switching the electrode polarity (LVSS) mode and the LVSS-sweeping mode were used to concentrate the anlaytes. Amoung these modes, the LVSS with switching polarity at 0.4 min was the optimal condition. The limits of detections (S/N = 3) of hesperetin、naringenin、quercetin and kaempferol were determined to be 14.87、14.71、19.76 and 16.66 ng/mL, respectively. In the second part, the sweeping technique was improved to concentrate the analytes. With 120 sec sample injection, the concentration of phosphate buffer at 20 mM was used as the sample matrix, while the separation buffer consisting of 20 mM phosphate electrolyte and 50 mM SDS and 10 % acetonitrile at pH 2.0 was optimized, and the limits of detections (S/N = 3) of hesperetin、naringenin、quercetin and kaempferol were determined to be 43.53、38.25、57.38 and 48.55 ng/mL, respectively. Sample injection up to 480 sec can also be achieved for baseline separation of four flavonoids in the sweeping mode, and the limits of detections (S/N = 3) of hesperetin、naringenin、quercetin and kaempferol were determined to be 21.28、15.37、24.26 and 24.7 ng/mL, respectively. The method was successfully applied to determine flavonoids in several real samples. | en |
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| dc.description.tableofcontents | 審定書 i
謝誌 ii 中文摘要 iv Abstract v 第1章 序論 1 1-1 引言 1 1-2 毛細管電泳發展史 1 1-3 毛細管電泳的分離原理 3 1-3-1 電泳遷移率(Electrophoretic mobility) 3 1-3-2 電滲流(Electroosmotic flow, EOF)與流型(Flow profile) 5 1-3-3 分離效率(Separation efficiency) 6 1-4 毛細管電泳的分離模式 8 1-4-1 毛細管區帶電泳法(CZE) 8 1-4-2 微胞電動力層析法(MEKC) 9 1-4-3 毛細管凝膠電泳法(CGE) 9 1-4-4 毛細管等電聚焦電泳法(CIEF) 10 1-4-5 毛細管等速電泳法(CITP) 10 1-4-6 毛細管電層析法(CEC) 10 1-5 毛細管電泳的注入方式 11 1-5-1 水動力注入法(Hydrodynamic injection) 11 1-5-2 水靜力注入法(Hydrostatic injection) 11 1-5-3 電動力注入法(Electrokinetic injection) 12 1-6 毛細管電泳的儀器裝置 13 1-7 線上樣品濃縮的原理與相關計算 13 1-8 毛細管電泳之線上樣品濃縮技術 16 1-8-1 正向堆積模式(NSM) 17 1-8-2 電極極性反向堆積模式(REPSM) 18 1-8-3 反向遷移微胞濃縮(SRMM) 18 1-8-4 增強電場樣品進樣(FESI) 19 1-8-5 以反向遷移微胞增強電場樣品進樣(FESI-RMM) 19 1-8-6 利用反向遷移微胞濃縮與水之堆積(SRW) 19 1-8-7 掃集(Sweeping) 20 1-8-8 陽離子選擇性注射法與掃集之結合模式(CSEI-Sweeping) 21 1-8-9 動力pH接合-掃集(dynamic pH junction-Sweeping) 21 1-9 毛細管電泳之未來展望 22 第2章 文獻探討 40 2-1 類黃酮類化合物 40 2-1-1 基本介紹 40 2-1-2 結構及分類 40 2-1-3 我們選用之分析物 42 2-2 類黃酮化合物之毛細管電泳相關文獻 43 第3章 實驗設備與方法 51 3-1 實驗藥品 51 3-1-1 分析物 51 3-1-2 緩衝溶液 51 3-1-3 緩衝溶液添加劑 52 3-1-4 其他試劑與藥品 52 3-2 實驗設備及耗材 52 3-2-1 毛細管電泳儀 52 3-2-2 毛細管柱 53 3-2-3 實驗室型酸鹼度/氧化還原電位計(Laboratory pH Meter) 54 3-2-4 實驗室型導電度計(Laboratory conductivity meter) 54 3-3 實驗方法 55 3-3-1 分析物之配製 55 3-3-2 緩衝溶液之配置 55 3-3-3 紅酒樣品之萃取 56 3-3-4 蜂蜜樣品之萃取 56 3-3-5 毛細管的處理 56 3-3-6 實驗操作 57 3-3-7 毛細管電泳之相關計算 57 第4章 結果與討論 61 4-1 線上濃縮模式之挑選 61 4-2 一般模式-毛細管區帶電泳法(CZE) 62 4-3 大體積樣品堆積(LVSS) 62 4-4 正向大體積堆積掃集 (LVSS-Sweeping) 63 4-5 正向掃集模式 (sweeping with normal polarity) 64 4-5-1 pH值 64 4-5-2 注入時間 64 4-6 逆向掃集模式 (sweeping with reverse polarity) 64 4-6-1 不同秒數的樣品注入變化 64 4-6-2 120秒樣品注入 65 4-6-3 480秒注入 67 4-7 各方法比較 68 4-8 結論 69 參考文獻 111 | |
| dc.language.iso | zh-TW | |
| dc.subject | 大體積堆積 | zh_TW |
| dc.subject | 類黃酮化合物 | zh_TW |
| dc.subject | 毛細管電泳 | zh_TW |
| dc.subject | 線上濃縮 | zh_TW |
| dc.subject | 樣品掃集 | zh_TW |
| dc.subject | sweeping | en |
| dc.subject | large volume sample stacking | en |
| dc.subject | flavonoids | en |
| dc.subject | capillary electrophoresis | en |
| dc.subject | on-line concentration | en |
| dc.title | 類黃酮化合物在毛細管電泳法之分離與線上濃縮之研究 | zh_TW |
| dc.title | Studies of Separation and On-line Concentration of Flavonoids in Capillary Electrophoresis | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 博士 | |
| dc.contributor.coadvisor | 林敬二(Ching-Erh Lin) | |
| dc.contributor.oralexamcommittee | 林金全(King-Chuen Lin),吳劍侯(Chien-Hou Wu),桂椿雄(Chun-Hsiung Kuei) | |
| dc.subject.keyword | 類黃酮化合物,毛細管電泳,線上濃縮,樣品掃集,大體積堆積, | zh_TW |
| dc.subject.keyword | flavonoids,capillary electrophoresis,on-line concentration,sweeping,large volume sample stacking, | en |
| dc.relation.page | 116 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2012-08-17 | |
| dc.contributor.author-college | 理學院 | zh_TW |
| dc.contributor.author-dept | 化學研究所 | zh_TW |
| 顯示於系所單位： | 化學系 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-101-1.pdf | 3.37 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。