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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 洪傳揚 | |
dc.contributor.author | Hui-Min Oung | en |
dc.contributor.author | 翁惠敏 | zh_TW |
dc.date.accessioned | 2021-06-16T22:59:38Z | - |
dc.date.available | 2022-08-07 | |
dc.date.copyright | 2012-08-28 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-07 | |
dc.identifier.citation | 戶刈義次 (1963) 作物學試驗法. 東京農業技術學會印行 p159-176
Agrawal, G. K., Jwa, N. S., Iwahashi, H., and Rakwal, R. (2003). Importance of ascorbate peroxidases OsAPX1 and OsAPX2 in the rice pathogen response pathways and growth and reproduction revealed by their transcriptional profiling. Gene 322, 93-103. Apel, K., and Hirt, H. (2004). Reactive oxygen species: metabolism, oxidative stress, and signal transduction. Annu Rev Plant Biol 55, 373-99. Asada, K. (1992). Ascorbate peroxide-a hydrogen peroxide-scavenging enzyme in plant. Physiol Plant 85, 235-241. Asada, K. (2006). Production and scavenging of reactive oxygen species in chloroplasts and their functions. Plant Physiol 141, 391-6. Asai, T., Tena, G., Plotnikova, J., Willmann, M. R., Chiu, W. L., Gomez-Gomez, L., Boller, T., Ausubel, F. M., and Sheen, J. (2002). MAP kinase signalling cascade in Arabidopsis innate immunity. Nature 415, 977-83. Bailly, C., El-Maarouf-Bouteau, H., and Corbineau, F. (2008). From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. C R Biol 331, 806-14. Bashir, K., Nagasaka, S., Itai, R. N., Kobayashi, T., Takahashi, M., Nakanishi, H., Mori, S., and Nishizawa, N. K. (2007). Expression and enzyme activity of glutathione reductase is upregulated by Fe-deficiency in graminaceous plants. Plant Mol Biol 65, 277-84. Bastola, D. R., and Minocha, S. C. (1995). Increased putrescine biosynthesis through transfer of mouse ornithine decarboxylase cDNA in carrot promotes somatic embryogenesis. Plant Physiol 109, 63-71. Beauchamp, C., and Fridovich, I. (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels. Anal Biochem 44, 276-87. Bethke, P. C., Badger, M. R., and Jones, R. L. (2004). Apoplastic synthesis of nitric oxide by plant tissues. Plant Cell 16, 332-41. Boveris, A., Valdez, L. B., Zaobornyj, T., and Bustamante, J. (2006). Mitochondrial metabolic states regulate nitric oxide and hydrogen peroxide diffusion to the cytosol. Biochim Biophys Acta 1757, 535-42. Cabanas, M. J., Vazquez, D., and Modolell, J. (1978). Inhibition of ribosomal translocation by aminoglycoside antibiotics. Biochem Biophys Res Commun 83, 991-7. Chao, Y. Y., Hong, C. Y., and Kao, C. H. (2010). The decline in ascorbic acid content is associated with cadmium toxicity of rice seedlings. Plant Physiol Biochem 48, 374-81. Charles, S. A., and Halliwell, B. (1981). Light activation of fructose bisphosphatase in photosynthetically competent pea chloroplasts. Biochem J 200, 357-63. Chopra, I., and Roberts, M. (2001). Tetracycline antibiotics: mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol Mol Biol Rev 65, 232-60 ; second page, table of contents. Conte, S., Stevenson, D., Furner, I., and Lloyd, A. (2009). Multiple antibiotic resistance in Arabidopsis is conferred by mutations in a chloroplast-localized transport protein. Plant Physiol 151, 559-73. Conte, S. S., and Lloyd, A. M. (2010). The MAR1 transporter is an opportunistic entry point for antibiotics. Plant Signal Behav 5, 49-52. DalCorso, G., Farinati, S., Maistri, S., and Furini, A. (2008). How plants cope with cadmium: staking all on metabolism and gene expression. J Integr Plant Biol 50, 1268-1280. Das, S., Misra, R. C., Sinha, S. K., and Pattanaik, M. C. (2010). Variation in sensitivity to streptomycin-induced bleaching and dark-induced senescence of rice (O. sativa) and ragi (E. coracana) genotypes and their relationship with yield and adaptability. J Crop Sci Biotech 13, 257-265. Davey, M. W., Van Montagu, M., Inzé, D., Sanmartin, M., Kanellis, A., Smirnoff, N., Benzie, I. J. J., Strain, J. J., Favell, D., and Fletcher, J. (2000). Plant L-ascorbic acid: chemistry, function, metabolism, bioavailability and effects of processing. J Sci Food Agri 80, 825-860. Davis, B. D. (1987). Mechanism of bactericidal action of aminoglycosides. Microbiol Rev 51, 341-50. Dekeyser, R., Claes, B., Marichal, M., Van Montagu, M., and Caplan, A. (1989). Evaluation of selectable markers for rice transformation. Plant Physiol 90, 217-23. Delledonne, M. (2005). NO news is good news for plants. Curr Opin Plant Biol 8, 390-6. Delledonne, M., Xia, Y., Dixon, R. A., and Lamb, C. (1998). Nitric oxide functions as a signal in plant disease resistance. Nature 394, 585-8. DiRita, V. J., and Gelvin, S. B. (1987). Deletion analysis of the mannopine synthase gene promoter in sunflower crown gall tumors and Agrobacterium tumefaciens. Mol Gen Genet 207, 233-41. Drlica, K., and Zhao, X. (1997). DNA gyrase, topoisomerase IV, and the 4-quinolones. Microbiol Mol Biol Rev 61, 377-92. Duan, Z., Duan, H., He, Y., Song, J., Duan, Z., and Li, X. (2009). Effects of antibiotics on root of Arabidopsis thaliana. Genet Mol Biol 10. Elthon, T. E., and McIntosh, L. (1987). Identification of the alternative terminal oxidase of higher plant mitochondria. Proc Natl Acad Sci U S A 84, 8399-403. Errampalli, D., Patton, D., Castle, L., Mickelson, L., Hansen, K., Schnall, J., Feldmann, K., and Meinke, D. (1991). Embryonic lethals and T-DNA insertional mutagenesis in Arabidopsis. Plant Cell 3, 149-57. Foyer, C. H., Souriau, N., Perret, S., Lelandais, M., Kunert, K. J., Pruvost, C., and Jouanin, L. (1995). Overexpression of glutathione reductase but not glutathione synthetase leads to increases in antioxidant capacity and resistance to photoinhibition in poplar trees. Plant Physiol 109, 1047-57. Fridovich, I. (1995). Superoxide radical and superoxide dismutases. Annu Rev Biochem 64, 97-112. Gadjev, I., Stone, J. M., and Gechev, T. S. (2008). Programmed cell death in plants: new insights into redox regulation and the role of hydrogen peroxide. Int Rev Cell Mol Biol 270. Gatica-Arias, A., Farag, M. A., Stanke, M., Matousek, J., Wessjohann, L., and Weber, G. (2012). Flavonoid production in transgenic hop (Humulus lupulus L.) altered by PAP1/MYB75 from Arabidopsis thaliana L. Plant Cell Rep 31, 111-9. Gechev, T., Gadjev, I., Van Breusegem, F., Inze, D., Dukiandjiev, S., Toneva, V., and Minkov, I. (2002). Hydrogen peroxide protects tobacco from oxidative stress by inducing a set of antioxidant enzymes. Cell Mol Life Sci 59, 708-14. Gechev, T. S., and Hille, J. (2005). Hydrogen peroxide as a signal controlling plant programmed cell death. J Cell Biol 168, 17-20. Gechev, T. S., Van Breusegem, F., Stone, J. M., Denev, I., and Laloi, C. (2006). Reactive oxygen species as signals that modulate plant stress responses and programmed cell death. Bioessays 28, 1091-101. Gonzalez, A., Jimenez, A., Vazquez, D., Davies, J. E., and Schindler, D. (1978). Studies on the mode of action of hygromycin B, an inhibitor of translocation in eukaryotes. Biochim Biophys Acta 521, 459-69. Hagar, H., Ueda, N., and Shah, S. V. (1996). Role of reactive oxygen metabolites in DNA damage and cell death in chemical hypoxic injury to LLC-PK1 cells. Am J Physiol 271, F209-15. Halliwell, B. (1998). Can oxidative DNA damage be used as a biomarker of cancer risk in humans? Problems, resolutions and preliminary results from nutritional supplementation studies. Free Radic Res 29, 469-86. Halliwell, B., and Foyer, C. H. (1976). Ascorbic acid, metal ions and the superoxide radical. Biochem J 155, 697-700. Hancock, R. E. (1981). Aminoglycoside uptake and mode of action-with special reference to streptomycin and gentamicin. II. Effects of aminoglycosides on cells. J Antimicrob Chemother 8, 429-45. Harrington, C. R., O'Hara, D. M., and Reynolds, P. E. (1989). Characterisation of a monoclonal antibody and its use in the immunoaffinity purification of penicillin-binding protein 2' of methicillin-resistant Staphylococcus aureus. FEMS Microbiol Lett 53, 143-7. Hauptmann, R. M., Vasil, V., Ozias-Akins, P., Tabaeizadeh, Z., Rogers, S. G., Fraley, R. T., Horsch, R. B., and Vasil, I. K. (1988). Evaluation of selectable markers for obtaining stable transformants in the gramineae. Plant Physiol 86, 602-6. Hayashimoto, A., Li, Z., and Murai, N. (1990). A polyethylene glycol-mediated protoplast transformation system for production of fertile transgenic rice plants. Plant Physiol 93, 857-63. Heath, R. L., and Packer, L. (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Arch Biochem Biophys 125, 189-98. Holme, I. B., Brinch-Pedersen, H., Lange, M., and Holm, P. B. (2008). Transformation of different barley (Hordeum vulgare L.) cultivars by Agrobacterium tumefaciens infection of in vitro cultured ovules. Plant Cell Rep 27, 1833-40. Hong, C., Chao, Y., Yang, M., Cheng, S., Cho, S., and Kao, C. (2009a). NaCl-induced expression of glutathione reductase in roots of rice (Oryza sativa L.) seedlings is mediated through hydrogen peroxide but not abscisic acid. Plant Soil 320, 103-115. Hong, C. Y., Chao, Y. Y., Yang, M. Y., Cho, S. C., and Huei Kao, C. (2009b). Na+ but not Cl- or osmotic stress is involved in NaCl-induced expression of glutathione reductase in roots of rice seedlings. J Plant Physiol 166, 1598-606. Hong, C. Y., Hsu, Y. T., Tsai, Y. C., and Kao, C. H. (2007). Expression of ASCORBATE PEROXIDASE 8 in roots of rice (Oryza sativa L.) seedlings in response to NaCl. J Exp Bot 58, 3273-83. Hsu, Y. T., and Kao, C. H. (2007). Toxicity in leaves of rice exposed to cadmium is due to hydrogen peroxide accumulation. Plant Soil 298, 231-241. Iamtham, S., and Day, A. (2000). Removal of antibiotic resistance genes from transgenic tobacco plastids. Nat. Biotechnol 18, 1172-6. Iqbal, M. M., Nazir, F., Ali, S., Asif, M. A., Zafar, Y., Iqbal, J., and Ali, G. M. (2012). Over expression of rice chitinase gene in transgenic peanut (Arachis hypogaea L.) improves resistance against leaf spot. Mol Biotechnol 50, 129-36. Jansch, L., Kruft, V., Schmitz, U. K., and Braun, H. P. (1996). New insights into the composition, molecular mass and stoichiometry of the protein complexes of plant mitochondria. Plant J 9, 357-68. Jeon, J. S., Lee, S., Jung, K. H., Jun, S. H., Jeong, D. H., Lee, J., Kim, C., Jang, S., Yang, K., Nam, J., An, K., Han, M. J., Sung, R. J., Choi, H. S., Yu, J. H., Choi, J. H., Cho, S. Y., Cha, S. S., Kim, S. I., and An, G. (2000). T-DNA insertional mutagenesis for functional genomics in rice. Plant J 22, 561-70. Kaminaka, H., Morita, S., Tokumoto, M., Yokoyama, H., Masumura, T., and Tanaka, K. (1999). Molecular cloning and characterization of a cDNA for an iron-superoxide dismutase in rice (Oryza sativa L.). Biosci Biotechnol Biochem 63, 302-8. Kato, M., and Shimizu, S. (1987). Chlorophyll metabolism in higher plants. VII. Chlorophyll degradation in senescing tobacco leaves; phenolic-dependent peroxidative degradation. Can J Bot 65, 729-735. Kohanski, M. A., Dwyer, D. J., Hayete, B., Lawrence, C. A., and Collins, J. J. (2007). A common mechanism of cellular death induced by bactericidal antibiotics. Cell 130, 797-810. Kong, X., Zhang, D., Pan, J., Zhou, Y., and Li, D. (2012). Hydrogen peroxide is involved in nitric oxide-induced cell death in maize leaves. Plant Biol. doi:10.1111/j.1438-8677.2012.00598.x Kos, A., Kuijvenhoven, J., Wernars, K., Bos, C. J., Van den Broek, H.W.J., Pouwels, P. H., and Hondel, V. d. (1985). Isolation and characterization of the Aspergillus niger rrpC gene. . Gene 39, 231-238. Kumar, M., Basha, P. O., Puri, A., Rajpurohit, D., Randhawa, G. S., Sharma, T. R., and Dhaliwal, H. S. (2010). A candidate gene OsAPC6 of anaphase-promoting complex of rice identified through T-DNA insertion. Funct Integr Genomics 10, 349-58. Kwak, J. M., Nguyen, V., and Schroeder, J. I. (2006). The role of reactive oxygen species in hormonal responses. Plant Physiol 141, 323-9. Lange, M., Vincze, E., Moller, M. G., and Holm, P. B. (2006). Molecular analysis of transgene and vector backbone integration into the barley genome following Agrobacterium-mediated transformation. Plant Cell Rep 25, 815-20. Liu, W., Duan, Q., Liu, J., and Sun, Y. (2012). Agrobacterium-mediated genetic transformation of secondary somatic embryos in alfalfa. Sheng Wu Gong Cheng Xue Bao 28, 203-13. Liu, Y., Ren, D., Pike, S., Pallardy, S., Gassmann, W., and Zhang, S. (2007). Chloroplast-generated reactive oxygen species are involved in hypersensitive response-like cell death mediated by a mitogen-activated protein kinase cascade. Plant J 51, 941-54. Lushchak, V. I. (2011). Adaptive response to oxidative stress: Bacteria, fungi, plants and animals. Comp Biochem Physiol C Toxicol Pharmacol 153, 175-90. McKersie, B. D., Chen, Y., de Beus, M., Bowley, S. R., Bowler, C., Inze, D., D'Halluin, K., and Botterman, J. (1993). Superoxide dismutase enhances tolerance of freezing stress in transgenic alfalfa (Medicago sativa L.). Plant Physiol 103, 1155-63. Mittler, R. (2002). Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 7, 405-10. Mittler, R., Vanderauwera, S., Gollery, M., and Van Breusegem, F. (2004). Reactive oxygen gene network of plants. Trends Plant Sci 9, 490-8. Molina, I., Weber, K., Dos Santos, D. Y., and Ohlrogge, J. (2009). Transformation of a dwarf Arabidopsis mutant illustrates gibberellin hormone physiology and the function of a green revolution gene. Biochem Mol Biol Educ 37, 170-7. Neill, S., Bright, J., Desikan, R., Hancock, J., Harrison, J., and Wilson, I. (2008). Nitric oxide evolution and perception. J Exp Bot 59, 25-35. Nutter, R., Everett, N., Pierce, D., Panganiban, L., Okubara, P., Lachmansingh, R., Mascarenhas, D., Welch, H., Mettler, I., Pomeroy, L., Johnson, J., and Howard, J. (1987). Factors affecting the level of kanamycin resistance in transformed sunflower cells. Plant Physiol 84, 1185-92. op den Camp, R. G., Przybyla, D., Ochsenbein, C., Laloi, C., Kim, C., Danon, A., Wagner, D., Hideg, E., Gobel, C., Feussner, I., Nater, M., and Apel, K. (2003). Rapid induction of distinct stress responses after the release of singlet oxygen in Arabidopsis. Plant Cell 15, 2320-32. Owens, L. D. (1981). Characterization of kanamycin-resistant cell lines of Nicotiana tabacum. Plant Physiol 67, 1166-8. Padilla, I. M., and Burgos, L. (2010). Aminoglycoside antibiotics: structure, functions and effects on in vitro plant culture and genetic transformation protocols. Plant Cell Rep 29, 1203-13. Panda, S. K. (2007). Chromium-mediated oxidative stress and ultrastructural changes in root cells of developing rice seedlings. J Plant Physiol 164, 1419-28. Pankey, G. A., and Sabath, L. D. (2004). Clinical relevance of bacteriostatic versus bactericidal mechanisms of action in the treatment of Gram-positive bacterial infections. Clin Infect Dis 38, 864-70. Passardi, F., Penel, C., and Dunand, C. (2004). Performing the paradoxical: how plant peroxidases modify the cell wall. Trends Plant Sci 9, 534-40. Planchet, E., Jagadis Gupta, K., Sonoda, M., and Kaiser, W. M. (2005). Nitric oxide emission from tobacco leaves and cell suspensions: rate limiting factors and evidence for the involvement of mitochondrial electron transport. Plant J 41, 732-43. Potrykus, I., Paszkowski, J., Saul, M. W., Petruska, J., and Shillito, R. D. (1985). Molecular and general genetics of a hybrid foreign gene introduced into tobacco by direct gene transfer. Mol Gen Genet 199, 169-77. Poyton, R. O., and McEwen, J. E. (1996). Crosstalk between nuclear and mitochondrial genomes. Annu Rev Biochem 65, 563-607. Prashanth, S. R., Sadhasivam, V., and Parida, A. (2008). Over expression of cytosolic copper/zinc superoxide dismutase from a mangrove plant Avicennia marina in indica rice var Pusa Basmati-1 confers abiotic stress tolerance. Transgenic Res 17, 281-91. Rao, R. N., Allen, N. E., Hobbs, J. N., Jr., Alborn, W. E., Jr., Kirst, H. A., and Paschal, J. W. (1983). Genetic and enzymatic basis of hygromycin B resistance in Escherichia coli. Antimicrob Agents Chemother 24, 689-95. Romero-Puertas, M. C., Rodriguze-Serrano, M., Corpas, F. J., Gomez, M., Def Rio, L. A., and Sandalio, L. M. (2004). Cadmium-induced subcellular accumulation of O2•-and H2O2 in pea leaves. Plant Cell Environ 27, 1122-1134. Rouhier, N., and Jacquot, J. P. (2008). Getting sick may help plants overcome abiotic stress. New Phytol 180, 738-41. Sallaud, C., Meynard, D., van Boxtel, J., Gay, C., Bes, M., Brizard, J. P., Larmande, P., Ortega, D., Raynal, M., Portefaix, M., Ouwerkerk, P. B., Rueb, S., Delseny, M., and Guiderdoni, E. (2003). Highly efficient production and characterization of T-DNA plants for rice ( Oryza sativa L.) functional genomics. Theor Appl Genet 106, 1396-408. Sandalio, L. M., Rodriguez-Serrano, M., Romero-Puertas, M. C., and Del Rio, L. A. (2008). Imaging of reactive oxygen species and nitric oxide in vivo in plant tissues. Methods Enzymol 440, 397-409. Scandalios, J. G. (2005). Oxidative stress: molecular perception and transduction of signals triggering antioxidant gene defenses. Braz J Med Biol Res 38, 995-1014. Schaefer, D., Zryd, J. P., Knight, C. D., and Cove, D. J. (1991). Stable transformation of the moss Physcomitrella patens. Mol Gen Genet 226, 418-24. Shao, H. B., Chu, L. Y., Shao, M. A., Jaleel, C. A., and Mi, H. M. (2008). Higher plant antioxidants and redox signaling under environmental stresses. C R Biol 331, 433-41. Siedow, J. N., and Umbach, A. L. (2000). The mitochondrial cyanide-resistant oxidase: structural conservation amid regulatory diversity. Biochim Biophys Acta 1459, 432-9. Smirnoff, N., and Wheeler, G. L. (2000). Ascorbic acid in plants: biosynthesis and function. Crit Rev Biochem Mol Biol 35, 291-314. Sun, H., Huang, Q. M., and Su, J. (2005). Highly effective expression of glutamine synthetase genes GS1 and GS2 in transgenic rice plants increases nitrogen-deficiency tolerance. Zhi Wu Sheng Li Yu Fen Zi Sheng Wu Xue Xue Bao 31, 492-8. Tanaka, Y., Hibino, T., Hayashi Y. , Tanaka A., Kishitani S. , Takabe T. , Yokota S. , and T., T. (1999). Salt tolerance of transgenic rice overexpressing yeast mitochondrial Mn-SOD in chloroplast. Plant Sci 148, 131–138. Tomasz, A., Drugeon, H. B., de Lencastre, H. M., Jabes, D., McDougall, L., and Bille, J. (1989). New mechanism for methicillin resistance in Staphylococcus aureus: clinical isolates that lack the PBP 2a gene and contain normal penicillin-binding proteins with modified penicillin-binding capacity. Antimicrob Agents Chemother 33, 1869-74. Tsai, Y. C., Hong, C. Y., Liu, L. F., and Kao, C. H. (2005). Expression of ascorbate peroxidase and glutathione reductase in roots of rice seedlings in response to NaCl and H2O2. J Plant Physiol 162, 291-9. Tun, N. N., Santa-Catarina, C., Begum, T., Silveira, V., Handro, W., Floh, E. I., and Scherer, G. F. (2006). Polyamines induce rapid biosynthesis of nitric oxide (NO) in Arabidopsis thaliana seedlings. Plant Cell Physiol 47, 346-54. Umbach, A. L., and Siedow, J. N. (1993). Covalent and noncovalent dimers of the cyanide-resistant Aalternative oxidase protein in higher plant mitochondria and their relationship to enzyme activity. Plant Physiol 103, 845-854. Ushimaru, T., Nakagawa, T., Fujioka, Y., Daicho, K., Naito, M., Yamauchi, Y., Nonaka, H., Amako, K., Yamawaki, K., and Murata, N. (2006). Transgenic Arabidopsis plants expressing the rice dehydroascorbate reductase gene are resistant to salt stress. J Plant Physiol 163, 1179-84. Valvekens, D., Montagu, M. V., and Van Lijsebettens, M. (1988). Agrobacterium tumefaciens-mediated transformation of Arabidopsis thaliana root explants by using kanamycin selection. Proc Natl Acad Sci USA 85, 5536-40. Vanlerberghe, G. C., and McIntosh, L. (1992). Coordinate regulation of cytochrome and alternative pathway respiration in tobacco. Plant Physiol 100, 1846-51. Vanlerberghe, G. C., and McIntosh, L. (1994). Mitochondrial electron transport regulation of nuclear gene expression. Studies with the alternative oxidase gene of tobacco. Plant Physiol 105, 867-74. Vanlerberghe, G. C., Robson, C. A., and Yip, J. Y. (2002). Induction of mitochondrial alternative oxidase in response to a cell signal pathway down-regulating the cytochrome pathway prevents programmed cell death. Plant Physiol 129, 1829-42. Vranova, E., Inze, D., and Van Breusegem, F. (2002). Signal transduction during oxidative stress. J Exp Bot 53, 1227-36. Weisblum, B., and Davies, J. (1968). Antibiotic inhibitors of the bacterial ribosome. Bacteriol Rev 32, 493-528. Wendehenne, D., Pugin, A., Klessig, D. F., and Durner, J. (2001). Nitric oxide: comparative synthesis and signaling in animal and plant cells. Trends Plant Sci 6, 177-83. Zago, E., Morsa, S., Dat, J. F., Alard, P., Ferrarini, A., Inze, D., Delledonne, M., and Van Breusegem, F. (2006). Nitric oxide- and hydrogen peroxide-responsive gene regulation during cell death induction in tobacco. Plant Physiol 141, 404-11. Zaninotto, F., La Camera, S., Polverari, A., and Delledonne, M. (2006). Cross talk between reactive nitrogen and oxygen species during the hypersensitive disease resistance response. Plant Physiol 141, 379-83. Zottini, M., Formentin, E., Scattolin, M., Carimi, F., Lo Schiavo, F., and Terzi, M. (2002). Nitric oxide affects plant mitochondrial functionality in vivo. FEBS Lett 515, 75-8. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64801 | - |
dc.description.abstract | 潮霉素 (Hygromycin B, Hyg) 是水稻轉殖最常用的篩選用抗生素,當轉殖水稻攜帶有 hygromycin phosphotransferase (hpt) 基因即可將 Hyg 磷酸化,避免 Hyg 與核醣體 RNA 結合造成蛋白質不正常轉譯,反之,非轉殖水稻則會受到 Hyg 毒害而死亡,然而 Hyg 導致植物死亡的機制目前仍然未知。為了瞭解 Hyg 造成水稻死亡的機制,本試驗以不同濃度 Hyg處理水稻幼苗,並從生理、生化及分子層次分析水稻的反應。結果顯示水稻經過 Hyg 處理之後,地上部及根部生長皆嚴重受抑制, Evans blue染色顯示 Hyg 會造成植物死亡,同時 malondialdehyde (MDA) 及被羰基化蛋白質含量 (carbonylation) 提高,活性氧族 (reactive oxygen species, ROS) 包括超氧自由基 (O2.-) 、過氧化氫 (H2O2) 及氫氧自由基 (OH.) 在地上部及根部中也隨 Hyg 濃度增加而提高,進一步分析發現 H2O2 主要累積粒線體。以 ascorbate 及 imidazole 處理則可降低 Hyg 造成的傷害,這些結果皆證實 Hyg 會造成水稻產生氧化逆境,進而導致死亡。進一步測定水稻中抗氧化酵素之酵素活性,結果顯示 CuZnSOD 及 MnSOD 酵素活性均受明顯增加,ascorbate peroxide及 glutathione reductase (GR) 活性也分別受到 Hyg 誘導 3 倍和 2.6 倍。基因表現方面,位在葉綠體的 GR3 在 Hyg 處理後6-12 小時間表現量有明顯誘導,而粒線體兩種呼吸作用路徑中關鍵酵素 alternative oxidase I (AOX I) 及 cytochrome c oxidase II (COX II) 在處理 72 小時後蛋白質表現皆增加,基因表現分析結果顯示AOX I 的三種同功酵素中 AOX I a 基因表現量受 Hyg 高度誘導,而 COX II 之基因表現量則無顯著差異。外加 H2O2 及 Menadione 分析水稻基因表現量,結果顯示僅有 H2O2 會誘導 GR3 及 AOXIa 基因表現量,證明Hyg 誘導 GR3及 AOX1a 是因為H2O2累積所導致。綜合以上結果顯示 Hyg 會在葉綠體及粒線體誘導大量 ROS 造成氧化逆境,進而破壞呼吸作用及光合作用,最後導致水稻死亡。 | zh_TW |
dc.description.abstract | Hygromycin B (Hyg) is the antibiotics most frequenly used in rice transformation. Rice calli is sensitive to Hyg. Transgenic rice carrying hygromycin phosphotransferase (hpt) can phosphorylate Hyg to avoid protein mistranslation, subsequently possesses rice resistant to Hyg. However, mechanism of rice cells sensitive to Hyg remains unclear. To figure out the mechanism, rice seedlings were treated with different concentrations of Hyg and their effects on physiologycal, biochemical and molecular changes were examined. The toxic effect caused by Hyg was observed including the reduction of plant height and root length, protein content, and the increase of malondialdehyde (MDA), carbonylated proteins, as well as Evans blue uptake. Meanwhile, levels of hydrogen peroxide (H2O2), superoxide radical (O2.-), and hydroxyl radical (OH.) were also significantly enhanced by Hyg in shoots. Observation of the localization of H2O2 indicated that H2O2 was mainly accumulated in mitochondria and chloroplast. Conversely, pretreatment of rice with ascorbate or imidazole can attenuate the toxicity, further confirmed that toxic effects caused by Hyg were mediated by oxidative stress. In addition, analysis of the antioxidant enzyme activities showed that CuZnSOD and MnSOD were highly induced. The activities of APX and GR were also induced by 3 folds and 2.6 folds, respectively. Gene expression analysis revealed that the chloroplast-localized GR3 was notably increased after 6-12 hours of Hyg treatment. Studies on the enzymes crucial in the mitochondrial respiration pathway, i.e. alternative oxidase I (AOXI) and cytochrome c oxidase II (COX II), shown that both proteins were significantly induced by Hyg. Reverse-transcription PCR analysis indicated that AOXIa was highly induced by Hyg, but no significant difference was observed in COXII. Futhermore, exogenous application of H2O2 and superoxide generator (menadione) showed that the expression of GR3 and AOXIa were induced by H2O2, instead of superoxide. All the results indicated that Hyg-induced oxidative stress is due to the accumulation of ROS in chloroplast and mitochondria, thus resulted in cell death. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T22:59:38Z (GMT). No. of bitstreams: 1 ntu-101-R99623018-1.pdf: 2146471 bytes, checksum: 1b13569bae69f084857a71d84bac8073 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 目錄
口試委員審定書 I 誌謝 II 目錄 III 圖目錄 V 附圖附表目錄 VI 中文摘要 VII Abstract VIII 縮寫字對照表 X 壹、前人研究 1 一、 抗生素種類及應用 1 二、 抗生素殺菌機制 4 三、 氧化逆境對植物生長發育上的影響 4 四、 植物的抗氧化機制 5 五、 植物與一氧化氮 7 六、 活性氧族與細胞程序性死亡之關係 9 七、 粒線體呼吸作用 10 八、 抗生素對植物生長發育的影響 12 貳、研究目的 13 參、材料與方法 14 一、 植物材料的準備及生長條件 14 二、 植物生理與生化分析 14 2.1 株高及根長測定 14 2.2 蛋白質測定 14 2.3 細胞死亡程度測定 (Evans blue 染色) 14 2.4 脂質過氧化作用測定 15 2.5 羰基化蛋白質含量分析 15 2.6 O2.- 及H2O2染色分析 17 2.7 ROS及 NO組織位置分析 17 2.8 ROS (O2.-、H2O2、OH.)定量分析 19 2.9 抗氧化酵素活性分析 20 2.10 同功酵素染色分析 (Zymoraphy) 22 三、 基因表現分析 23 四、 外加 ASC 及IMD試驗 26 五、 統計分析 26 肆、結果 27 一、 Hygromycin B 對水稻外表形態及生理反應之影響 27 二、 利用外加 ASC 減緩 Hyg 毒性 30 三、 Hygromycin B 處理下抗氧化酵素活性及基因表現量之變化 30 四、 葉綠體光合作用基因表現與粒線體呼吸作用酵素相關基因表現與蛋白質含量分析 31 五、 外加 H2O2 及menadione 分析受 hygromycin B 誘導基因之表現 31 六、 抑制 NADPH oxidase 可減緩 hygromycin B 對水稻毒害 31 伍、討論 33 一、 Hygromycin B 與其它抗生素對植株生理特性影響 33 二、 Hygromycin B 對粒線體及葉綠體之影響 35 三、 水稻處理 IMD 及 ASC 可減緩 hygromycin B 毒性 36 四、 Hygromycin B 對水稻毒害之機制 36 陸、參考文獻 39 | |
dc.language.iso | zh-TW | |
dc.title | 潮酶素誘導水稻細胞死亡機制之研究 | zh_TW |
dc.title | Studies on the mechanism of cell death caused by hygromycin B in Oryza sativa L. | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 高景輝,李澤民,陳凱儀,許奕婷 | |
dc.subject.keyword | 潮霉素,氧化逆境,水稻,活性氧族,抗氧化酵素, | zh_TW |
dc.subject.keyword | Hygromycin B,oxidative stress,Oryza sativa L.,ROS,antioxidant enzymes , | en |
dc.relation.page | 69 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-08 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
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