青脆枝的微體繁殖

Abstract

以七月份採收未成熟的青脆枝種子，洗淨後以1%次氯酸鈉溶液超音波震盪處理10分鐘進行表面消毒，無汙染率84%。 青脆枝種子胚經無菌播種於MS培養基誘導無菌苗。再以無菌苗頂芽和莖段作為培殖體，置於MS培養基添加0.3mg/L TDZ，以頂芽誘導之多芽體發生率90%、莖段為83%。 多芽體切成1cm長的片段進行芽體抽長，以MS培養基添加0.2mg/L BA及0.1mg/L IBA促進芽體抽長，芽體抽長率87%，平均芽體長度4.2cm，之後以1/4MS培養基添加0.5mg/L IBA及1mg/L IAA誘導芽體發根，發根率達87%，平均發根數為每個培植體3根，平均根長4.6cm。 發根的芽體可用於馴化，馴化60天的成活率為55%。

The Immature Nothapodyes nimmoniana seeds were harvested in July. The seeds were sterilized sonicated for 10 min with 1% sodium hypochlorite aqueous solution after washing, the sterilization rate was 84%. The zygotic embryos were sown on MS medium in aseptic environment for cultivating aseptic seedlings. The apical buds and stem segment of Nothapodyes nimmoniana aseptic seedling were used as explants for multiple buds inducing test. The optimal formula for multiple buds inducting test was MS medium and 0.3mg/L PGR TDZ. The induting rate on apical buds and stem segment were 90% and 83% respectively. Multiple buds were cut into 1cm segment for shoot elongation. The optimal formula for shoot elongation was MS medium, 0.2mg/L BA and 0.1mg/L IBA. Elongated shoots were used as explants for roots induction. The optimal formula for root induction was 1/4MS, 0.5mg/L IBA and 1mg/L IAA. Shoots with root were used in adaptation. The survival rate was 55%.