請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64678
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 林順福 | |
dc.contributor.author | Ching-Hui Chung | en |
dc.contributor.author | 鍾淨惠 | zh_TW |
dc.date.accessioned | 2021-06-16T22:57:18Z | - |
dc.date.available | 2017-08-10 | |
dc.date.copyright | 2012-08-10 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-09 | |
dc.identifier.citation | 任保青、陳之瑞。2010。植物DNA條形碼技術。植物學報 45:1-12。
行政院農業委員會。2010。99年農業統計年報。行政院農業委員會編印。 李台強、張清寬。2003。台灣茶樹種原圖誌。pp.23-203。行政院農業委員會茶業改良場編印。 林亞平。2009。ISSR分子標誌與DNA條碼應用於茶葉之品種鑑定。國立台灣大學農藝學研究所碩士論文。 林亞平、胡智益、蔡右任、林順福。2010。成茶品種快速分子鑑定技術之研究及應用。作物、環境與生物資訊 7:37-51。 林順福。2005。作物品種之DNA鑑定技術。二十一世紀農業發展與新興科技應用研討會,pp.25-35。救國團/中華農學會編印。 胡智益。2004。台灣茶樹種原葉部性狀及DNA序列變異之探討。國立台灣大學農藝研究所碩士論文。 胡智益、郭冠黎、林順福、蔡右任。2005。台灣茶樹種原葉部性狀之調查及遺傳變異分析。台灣茶葉研究彙報 24:1-20。 胡智益、蔡右任;林順福。2006。成茶之品種分子鑑定可行性評估。中華農學會報 7:499-510。 胡智益、蔡右任、林順福。2011。DNA分子標誌應用在茶樹之現況與潛力。農業生技產業季刊 25:30-37。 胡智益、蔡憲宗、蔡右任、林順福。2010。台灣茶樹經濟栽培品種之成茶鑑定。第六屆海峽兩岸茶業學術研討會論文集,pp.18-24。 楊美珠。2004。茶樹嫁接扦插苗之改良應用與展望。農政與農情 144:92-94。 劉振、姚明哲、王新超、陳亮。2009。基於EST-SSR的福建地區茶樹資源遺傳多樣性和親緣關係分析。中國農業科學 45:1720-1727。 Afridi S. G., Ahmad H., Alam M., Khan I. A. and Hassan M. 2011. DNA landmarks for genetic diversity assessment in tea genotypes using RAPD markers. Afr. J. Biotechnol. 10:15477-15482. Alvarez I. and Wendel J. F. 2003. Ribosomal ITS sequences and plant phylogenetic inference. Mol. Phylogenet. Evol. 29:417-434. Borchetia S., Das S. C., Handique P. J. and Das S. 2009. High multiplication frequency and genetic stability for commercialization of the three varieties of micropropagated tea plants (Camellia spp.). Sci. Hortic. 120:544-550. CBOL Plant Working Group. 2009. A DNA barcode for land plants. Proc. Natl. Acad. Sci. U. S. A. 106:12794-12797. Chen J., Wang P., Xia Y., Xu M. and Pei S. 2005. Genetic diversity and differentiation of Camellia sinensis L. (cultivated tea) and its wild relatives in Yunnan province of China, revealed by morphology, biochemistry and allozyme studies. Genet. Resour. Crop Evol. 52:41-52. Chen L. and Yamaguchi S. 2002. Genetic diversity and phylogeny of tea plant (Camellia sinensis) and its related species and varieties in the section Thea genus Camellia determined by randomly amplified polymorphic DNA analysis. J. Horti. Sci. Biotechnol. 77:729-732. Chen L. and Yamaguchi S. 2005. RAPD markers for discriminating tea germplasms at the inter-specific level in China. Plant Breeding 124:404-409. Chen S., Yao H., Han J., Liu C., Song J., Shi L., Zhu Y., Ma X., Gao T., Pang X., Luo K., Li Y., Li X., Jia X., Lin Y. and Leon C. 2010. Validation of the ITS2 region as a novel DNA barcode for identifying medicinal plant species. PLoS ONE 5:e8613. Chen Y., Yu M., Xu J., Chen X. and Shi J. 2009. Differentiation of eight tea (Camellia sinensis) cultivars in China by elemental fingerprint of their leaves. J. Sci. Food Agric. 89:2350-2355. China Plant BOL Group. 2011. Comparative analysis of a large dataset indicates that internal transcribed spacer (ITS) should be incorporated into the core barcode for seed plants. Proc. Natl. Acad. Sci. U.S.A. 108:19641-19646. Cho K. H., Lee E. J., Tsuge T., Jo A., Kim J. C., Cheong G. W., Yoon H. S. and Kim G. T. 2010. Comparative genomic analysis of Korean and Japanese green tea trees by using molecular markers. Can. J. Plant Sci. 90:239-298. Cho Y., Mower J. P., Qiu Y. L. and Palmer J. D. 2004. Mitochondrial substitution rates are extraordinarily elevated and variable in a genus of flowering plants. Proc. Natl. Acad. Sci. U.S.A. 101:17741-17746. Costion C., Ford A., Cross H., Crayn D., Harrington M. and Lowe A. 2011. Plant DNA barcodes can accurately estimate species richness in poorly known floras. PLoS ONE 6:e26841. Devarumath R. M., Nandy S., Rani V., Marimuthu S., Muraleedharan N. and Raina S. N. 2002. RAPD, ISSR and RFLP fingerprints as useful markers to evaluate genetic integrity of micropropagated plants of three diploid and triploid elite tea clones representing Camellia sinensis (China type) and C. assamica ssp. assamica (Assam-India type). Plant Cell Rep. 21:166-173. Doyle J. J. and Doyle J. L. 1990. Isolation of plant DNA from fresh tissue. Focus 12:13-15. Drumwright A. M., Allen B. W., Huff K. A., Ritchey P. A. and Cahoon A. B. 2011. Survey and DNA barcoding of Poaceae in Flat Rock Cedar Glades and Barrens State Natural Area, Murfreesboro, Tennessee. Castanea 76:300-310. Dumolin-Lapegue S., Pemonge M. H. and Petit R. J. 1997. An enlarged set of consensus primers for the study of organelle DNA in plants. Mol. Ecol. 6:393-397. Ercisli S., Orhan E., Ozdemir O., Sengul M. and Gungor N. 2008. Seasonal variation of total phenolic, antioxidant activity, plant nutritional elements, and fatty acids in tea leaves (Camellia sinensis var. sinensis clone Derepazari 7) grown in Turkey. Pharm. Biol. 46:683-687 Gao T. and Chen S. L. 2009. Authentication of the medicinal plants in Fabaceae by DNA barcoding technique. Planta Med. 75:417-417. Gao T., Yao H., Song J., Liu C., Zhu Y., Ma X., Pang X., Xu H. and Chen S. 2010. Identification of medicinal plants in the family Fabaceae using potential DNA barcode ITS2. J. Ethnopharmacol. 130:116-121. Guo X., Wang X., Su W., Zhang G. and Zhou R. 2011. DNA barcodes for discriminating the medicinal plant Scutellaria baicalensis (Lamiaceae) and its adulterants. Biol. Pharm. Bull. 34:1198-1203. Hebert P. D. N., Cywinska A., Ball S. L. and deWaard J. R. 2003. Biological identifications through DNA barcodes. Proc. Biol. Sci. 270:313-321. Hsiao C., Jacobs S. W. L., Chatterton N. J. and Asay K. H. 1999. A molecular phylogeny of the grass family (Poaceae) based on the sequences of nuclear ribosomal DNA (ITS). Aust. Syst. Bot. 11:667-688. Hung C. Y., Wang K. H., Huang C. C., Gong X., Ge X. J. and Chiang T. Y. 2008. Isolation and characterization of 11 microsatellite loci from Camellia sinensis in Taiwan using PCR-based isolation of microsatellite arrays (PIMA). Conserv. Genet. 9:779-781. Jaakola L., Suokas M. and Haggman H. 2010. Novel approaches based on DNA barcoding and high-resolution melting of amplicons for authenticity analyses of berry species. Food Chem. 123:494-500. Kafkas S., Ercişli S., Doğan Y., Erturk Y., Haznedar A. and Sekban R. 2009. Polymorphism and genetic relationships among tea genotypes from Turkey revealed by amplified fragment length polymorphism markers. J. Am. Soc. Hortic. Sci. 134:428-434. Karthigeyan S., Rajkumar S., Sharma R. K., Gulati A., Sud R. K. and Ahuja P. S. 2008. High level of genetic diversity among the selected accessions of tea (Camellia sinensis) from abandoned tea gardens in western Himalaya. Biochem. Genet. 46:810-819. Katoh Y., Katoh M., Takeda Y. and Omori M. 2003. Genetic diversity within cultivated teas based on nucleotide sequence comparison of ribosomal RNA maturase in chloroplast DNA. Euphytica 134:287-295. Kaundun S. S. and Matsumoto S. 2002. Heterologous nuclear and chloroplast microsatellite amplification and variation in tea, Camellia sinensis. Genome 45:1041-1048. Kaundun S. S. and Matsumoto S. 2003. Development of CAPS markers based on three key genes of the phenylpropanoid pathway in Tea, Camellia sinensis (L.) O. Kuntze, and differentiation between assamica and sinensis varieties. Theor. Appl. Genet. 106:375-383. Kaundun S. S., Zhyvoloup A. and Park Y. G. 2000. Evaluation of the genetic diversity among elite tea (Camellia sinensis var. sinensis) accessions using RAPD markers. Euphytica 115:7-16. Lai J. A., Yang W. C. and Hsiao J. Y. 2001. An assessment of genetic relationships in cultivated tea clones and native wild tea in Taiwan using RAPD and ISSR markers. Bot. Bull. Acad. Sinica 42:93-100. Li M., Wong K. L., Chan W. H., Li J. X., But P. P. H., Cao H. and Shaw P. C. 2012. Establishment of DNA barcodes for the identification of the botanical sources of the Chinese ‘cooling’ beverage. Food Control 25:758-766. Magoma G. N , Wachira F. N., Obanda M., Imbuga M. and Agong S. G. 2000. The use of catechins as biochemical markers in diversity studies of tea (Camellia sinensis). Genet. Resour. Crop Evol. 47:107-114. Mariotti R., Cultrera N. G. M., Diez C. M., Baldoni L. and Rubini A. 2010. Identification of new polymorphic regions and differentiation of cultivated olives (Olea europaea L.) through plastome sequence comparison. BMC Plant Biol. 10:211. Matsumoto S., Kiriiwa Y. and Takeda Y. 2002. Differentiation of Japanese green tea cultivars as revealed by RFLP analysis of phenylalanine ammonia lyase DNA. Theor. Appl. Genet. 104:998-1002. Matsumoto S., Takeuchi A., Hayatsu M. and Kondo S. 1994. Molecular cloning of phenylalanine ammonia-lyase cDNA and classification of varieties and cultivars of tea plants (Camellia sinensis) using the tea PAL cDNA probe. Theor. Appl. Genet. 89:671-675. Mayer M. S. and Bagga S. K. 2002. The phylogeny of Lens (Leguminosae): new insight from ITS sequence analysis. Plant Syst. Evol. 232:145-154. Mondal T. K. 2002. Assessment of genetic diversity of tea (Camellia sinensis (L.) O. Kuntze) by inter simple sequence repeat polymerase chain reaction. Euphytica 128:307-315. Ohsako T., Ohgushi T., Motosugi H. and Oka K. 2008. Microsatellite variability within and among local landrace populations of tea, Camellia sinensis (L.) O. Kuntze, in Kyoto, Japan. Genet. Resour. Crop Evol. 55:1047-1053. Paul S., Wachira F. N., Powell W. and Waugh R. 1997. Diversity and genetic differentiation among populations of Indian and Kenyan tea (Camellia sinensis (L.) O. Kuntze) revealed by AFLP markers. Theor. Appl. Genet. 94:255-263. Sharma R. K., Negi M. S., Sharma S., Bhardwaj P., Kumar R., Bhattachrya E., Tripathi S. B., Vijayan D., Baruah A. R., Das S. C., Bera B., Rajkumar R., Thomas J., Sud R. K., Muraleedharan N., Hazarika M., Lakshmikumaran M., Raina S. N. and Ahuja P. S. 2010. AFLP-based genetic diversity assessment of commercially important tea germplasm in India. Biochem. Genet. 48:549-564. Starr J. R., Naczi R. F. C. and Chouinard B. N. 2009. Plant DNA barcodes and species resolution in sedges (Carex, Cyperaceae). Mol. Ecol. Resour. 9:151-163. Steele P. R., Friar L. M., Gilbert L. E. and Jansen R. K. 2010. Molecular systematics of the neotropical genus Psiguria (Cucurbitaceae): implications for phylogeny and species identification. Am. J. Bot. 97:156-173. Stoeckle M. Y., Gamble C. C., Kirpekar R., Young G., Ahmed S. and Little D. P. 2011. Commercial teas highlight plant DNA barcode identification successes and obstacles. Sci. Rep. 1:42. Takeo T. 1992. Green and semi-fermented teas. In: Tea. (ed. By Willson KC and Clifford MN). pp.413-457. Chapman & Hall Press, London. Thomas J., Vijayan D., Joshi S. D., Lopez S. J. and Kumar R. R. 2006. Genetic integrity of somaclonal variants in tea (Camellia sinensis (L.) O Kuntze) as revealed by inter simple sequence repeats. J. Biotechnol. 123:149-154. Wachira F. N., Waugh R., Hackett C. A. and Powell W. 1995. Detection of genetic diversity in tea (Camellia sinensis) using RAPD markers. Genome 38:201-210. Wang K. R., Du Y. Y., Shao S. H., Lin C., Ye Q., Lu J. L. and Liang Y. R. 2010. Development of specific RAPD markers for identifying albino tea cultivars 'Qiannianxue' and 'Xiaoxueya'. Afr. J. Biotechnol. 9:434-437. Wei K., Wang L., Zhou J., He W., Zeng J., Jiang Y. and Cheng H. 2011. Catechin contents in tea (Camellia sinensis) as affected by cultivar and environment and their relation to chlorophyll contents. Food Chem. 125:44-48. Zietkiewicz E., Rafalski A. and Labuda D. 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics 20:176-183. Zhao L. P., Liu Z., Chen L., Yao M. Z. and Wang X. C. 2008. Generation and characterization of 24 novel EST derived microsatellites from tea plant (Camellia sinensis) and cross-species amplification in its closely related species and varieties. Conserv. Genet. 9:1327-1331. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64678 | - |
dc.description.abstract | 茶樹為我國重要之經濟作物,因應各地不同的產製環境而發展出各種特色茶。近年發現有進口低價茶葉冒充或混雜國產特色茶之情況,甚或將進口茶葉冒充本國茶葉參加各特色茶區之比賽,為維護我國各特色茶區之發展,有必要區隔國產與進口茶葉。本研究以碧螺春及東方美人茶為研究對象,以M8R (細胞質DNA)及ITS2F (細胞核內DNA)兩組DNA條碼進行茶園茶樹樣品、市售茶、比賽茶及進口茶葉之品種鑑定。收集自碧螺春主要產區之市售茶及比賽茶均為青心柑仔品種製成,茶園可疑樣品之DNA條碼分析結果顯示尖葉種具有與青心柑仔相同之DNA序列,經ISSR DNA分子標誌分析後亦未發現多型性,另一懷疑為台茶16號之茶樹經DNA條碼分析後推測為台茶14號及台茶17號之雜交實生苗,來自青心柑仔與紅心大冇混植茶園之兩可疑樣品則分別為雜交所得之實生苗及青心柑仔變異種。而在東方美人茶主要產區內,大多數茶園所種植之品種為青心大冇品種,大部分比賽茶也為青心大冇品種所製成,但仍有少數東方美人茶係以台茶12號製成,另外亦發現一個核內DNA序列具有1個SNP變異之青心大冇變異種。DNA序列分析結果亦顯示中國進口之碧螺春係由青心柑仔或台茶12號品種製成,而進口之毛蟹品種則是以青心烏龍品種製成。此外本研究也建立黃柑、柑仔(黃)、紅心柑仔、烏金、武夷及大葉烏龍等茶樹品種之DNA指紋,可供未來品種鑑定之參考。本研究顯示以DNA條碼進行茶葉品種鑑定之效果良好,未來可配合產銷履歷制度進行特色茶葉產品區隔,以增進國產特色茶之發展。 | zh_TW |
dc.description.abstract | Tea (Camellia sinensis) is one of the most important economic crops in Taiwan. Because of different growing and producing environments, each tea production area has its own unique tea. In recent years, some fake or adulterant imported tea varieties were found in the domestic markets or competition tea activities. To maintain the industry of unique tea in Taiwan, it’s necessary to distinguish domestic tea from imported tea. In this study, tea samples of Pi-Lou-Chun and Oriental Beauty Tea were analyzed by using M8R (organelle DNA) and ITS2F (nuclear DNA) DNA barcodes. These samples were collected from main production areas, markets, competition tea activities and China. All tea samples collected from main production areas of Pi-Lou-Chun and markets were verified as the variety Chin-Shin-Gan-Tzy. A mutant with acicular buds was identified with the same DNA sequences and ISSR DNA fingerprints as Chin-Shin-Gan-Tzy. One tea tree of suspected variety TTES No. 16 was identified as natural crossing between TTES No.14 and TTES NO. 17. Two suspected samples from tea garden of mixture planting of Chin-Shin-Gan-Tzy and Horng-Shin-Dah-Pan were a mutant of Chin-Shin-Gan-Tzy and a hybrid of these two varieties, respectively. Most plants grown in the main production areas of Oriental Beauty Tea were variety Chin-Shin-Dah-Pan, and most competition tea samples of Oriental Beauty Tea were identified as variety Chin-Shin-Dah-Pan. But some Oriental Beauty Tea samples were made by variety TTES NO. 12. Besides, a few samples of Chin-Shin-Dah-Pan with one SNP in nuclear DNA sequence were found. Imported tea labeled “Pi-Lou-Chun” was made of Chin-Shin-Gan-Tzy or TTES NO. 12, and imported tea samples of Mau-Hsei were verified to be made of variety Chin-Shin-Oolong. In addition, DNA fingerprints of varieties Hwang-Gan, Gan-Yzy (Yellow), Horng-Shin-Gan-Tzy, Wu-Jin, Wuu-Yi and Dah-Yeh-Oolong were established. This study has shown that DNA barcodes are very useful in variety identification of tea. For enhancing the unique tea industry in Taiwan, DNA barcodes could be applied with traceability system in the future. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T22:57:18Z (GMT). No. of bitstreams: 1 ntu-101-R99621108-1.pdf: 4032409 bytes, checksum: 2b2902a75f68190b064f180746212dbf (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 口試委員審定書...........................................................................i
誌謝...........................................................................................ii 中文摘要....................................................................................iii Abstract.....................................................................................iv 目錄...........................................................................................vi 表目錄......................................................................................viii 圖目錄.......................................................................................ix 一、前言....................................................................................1 二、前人研究.............................................................................3 三、材料與方法..........................................................................8 1. 碧螺春茶區青心柑仔變異種之外觀辨識及DNA指紋分析...............8 2. 碧螺春茶區其他非青心柑仔品種之DNA指紋鑑定.........................8 3. 碧螺春比賽茶、市售茶及進口茶葉之品種鑑定............................9 4. 東方美人茶茶園品種鑑定.........................................................9 5. 其他適製東方美人茶之茶樹品種之DNA指紋建立........................9 6. 進口東方美人茶之品種鑑定.....................................................10 7. 東方美人茶比賽茶之品種鑑定..................................................10 8. DNA萃取...............................................................................13 9. 聚合酶連鎖反應.....................................................................13 10. 電泳分析.............................................................................14 11. DNA定序及序列分析.............................................................14 四、結果...................................................................................17 1. 碧螺春茶區青心柑仔變異種之外觀辨識及DNA指紋分析..............17 2. 碧螺春茶區其他非青心柑仔品種之DNA指紋鑑定........................22 3. 碧螺春比賽茶、市售茶及進口茶葉之品種鑑定...........................23 4. 東方美人茶茶園品種鑑定.........................................................30 5. 其他適製東方美人茶之茶樹品種之DNA指紋建立........................30 6. 進口東方美人茶之品種鑑定......................................................31 7. 東方美人茶比賽茶之品種鑑定...................................................31 五、討論....................................................................................39 1. 碧螺春茶區青心柑仔變異種之外觀辨識及DNA指紋分析...............39 2. 碧螺春茶區其他非青心柑仔品種之DNA指紋鑑定.........................40 3. 碧螺春比賽茶、市售茶及進口茶葉之品種鑑定............................41 4. 東方美人茶茶園品種鑑定..........................................................41 5. 其他適製東方美人茶之茶樹品種之DNA指紋建立.........................43 6. 進口東方美人茶之品種鑑定......................................................44 7. 東方美人茶比賽茶之品種鑑定...................................................44 8. 綜合討論.................................................................................45 六、結論.....................................................................................47 七、參考文獻..............................................................................48 | |
dc.language.iso | zh-TW | |
dc.title | DNA條碼應用於碧螺春及東方美人茶之品種鑑定 | zh_TW |
dc.title | The Applications of DNA Barcodes in Variety Identification of Pi-Lou-Chun and Oriental Beauty Tea | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 葉茂生,曾富生,李瑞興,盧煌勝 | |
dc.subject.keyword | 茶,品種鑑定,DNA條碼,DNA指紋,ITS, | zh_TW |
dc.subject.keyword | tea,variety identification,DNA barcode,DNA fingerprint,ITS, | en |
dc.relation.page | 54 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-09 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-101-1.pdf 目前未授權公開取用 | 3.94 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。