飼料中添加北蛹蟲草對青魚成長與免疫反應之影響

Abstract

本篇研究探討添加北蛹蟲草子實體與固態培養基的菌絲體對青魚成長、免疫及血液影響。實驗分為兩部分，實驗一添加北蛹蟲草子實體0%、0.25%、0.5%、1%及2%對青魚成長與免疫的影響。實驗二添加北蛹蟲草菌絲體培養米基0％、1.25％、2.5％、5％及10%對青魚成長與免疫的影響。 實驗一的魚體平均初重5.05±0.17g，各組三重複，每日餵食二次，飼養八週。實驗一的結果顯示添加1%增重的效果最好，達到顯著差異(p＜0.05)。超氧陰離子(O2‾)在飼養16天後於添加1%的為最高。在飼養32天後於添加2%的為最高。在飼養56天後於添加0.5%的值為最高，達到顯著差異(p＜0.05)。吞噬指標在第16天無顯著差異，第32天以添加0.25%為最好，第56天以添加2%為最好，皆明顯高於對照組(p＜0.05)。溶菌酵素在第16天以添加0.5%最好。第32天及第56天以添加0.25%最佳。 實驗二的魚體初重5.55±0.24g，結果顯示添加蟲草米基1.25%的成長與增重效果較佳，統計上各組在增重率無顯著差異(p>0.05)。超氧陰離子(O2‾)在飼養16天後添加2.5%的值最高。在飼養32天及56天於M10組值最高。吞噬指標在第16天以添加1.25%的組為最高，第32天及第56天以添加10%為最高，皆與對照組達到顯著差異(p＜0.05)。溶菌酵素活性在第16天時添加10%最佳，第32天時2.5、%的組別最佳，第56天時以添加2.5%最佳，皆高於對照組且達到顯著差異。

To know the effects on growth, and non-specific immune responses from dietary supplement of fruiting bodies of Cordyceps militaris and rice medium of Cordyceps militaris on black carps（Mylopharyngodon piceus). In the following experiment will be in two parts, first experiment, adding fruiting bodies of Cordyceps militaris as dietary supplement on a dose-dependent 0, 0.25, 0.5, 1 and 2% as weight as total feeds. Second experiment, dose-dependent effects of rice medium of Cordyceps militaris were added as 0, 1.25, 2.5, 5 and 10% as total weight of feeds. In experiment I, initial weight of 5.05±0.17g black carps were selected and fed twice a day for continuously 8 weeks. The result shows that the precent weight gain (PWG) of fish feeding diet with 1% fruiting bodies was significantly higher than other-does-treatment. In non-specific immune response of black carp, fish feeding diet containing 1, 2, 0.5% weight of fruiting bodies have the highest superoxide anion (O2-) production rate than other treatments after 16, 32, 56 days, respectively. The groups have the highest phagocytic index were fed with 0.25, 2% fruiting bodies in feeding after 32, 56 days, respectively, however, it shows no significant differences in phagocytic index between control and experiments groups in 16 days. The lysozyme activity in 16, 32 and 56 days, shows adding with 0.5, 0.25 and 0.25 fruiting bodies have the highest efficiency. In experiment II, initial weight was 5.55±0.24g were selected to treated with rice medium. The result shows that the precent weight gain (PWG) of fish feeding diet with 1.25% rice medium is the best among all the treatments, but have no significant differences. In non-specific immune responses of black carp, fish feeding diet with 2.5, 10 and 10% rice medium as weight as total feeds have the highest superoxide anion (O2-) production rate compare to each treatments in 16, 32 and 56 days, respectively. In phagocytic index, the feeding treatment with 1.25, 10 and 10% rice medium have the highest phagocytic index among each groups in 16, 32, 56 days, respectively. The lysozyme activity in 16 days showed highest in 10% weight rice medium feeding, and in 32 and 56 days treatment, 5 and 2.5% rice medium feeding have the highest activity.