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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 陳敏慧(Min-huey Chen) | |
| dc.contributor.author | Sheng-Lin Teng | en |
| dc.contributor.author | 鄧聖霖 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:56:51Z | - |
| dc.date.available | 2017-09-17 | |
| dc.date.copyright | 2012-09-17 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-12 | |
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64594 | - |
| dc.description.abstract | 牙髓幹細胞是種多潛性體細胞(Multipotent stromal cell)能夠被分化為硬 骨,軟骨,脂肪,神經細胞。由於較容易取於自體,擁有倫理爭議較小,且 較沒有排斥問題等優勢。
本實驗中利用聚乙烯醇基材(polyvinyl-alcohol),讓細胞呈現懸浮且聚 集呈團的情況。觀察這樣懸浮且聚集的細胞團是否有神經分化上的改變。 經懸浮培養的細胞團,再以 TCPS,poly-D-lysine,collagen 的不同基材 (substrate)培養,觀察對神經分化是否造成影響。 在誘導分化上,則使用 PKC activator TPA,all-trans retinoic acid,dbcAMP, IBMX 及 BDNF,NT-3 神經生長因子。在無血清環境下,搭配 ITS,B27 補 充劑。首次併用 retinoic acid 及 PKC activator 兩個不同的誘導系統。 實驗結果發現,聚集呈團的細胞即使在沒有分化誘導的組別也可以看到 RNA 表現量改變。使用免疫螢光染色,也有 Nestin,βIII-Tubulin,FOX3, NFM 等神經蛋白標記。若有神經分化誘導的組別表現量則更加明顯。 而懸浮再貼附的組別,發現在分化誘導組別中,大部份細胞都會表現上 述幾種蛋白,無論是已散開貼平的細胞團或未完全散開的細胞團。但在未加 以分化誘導的組別,則僅呈現在未完全散開的細胞團。誘導狀況下懸浮後貼 附在 poly-D-lysine 上十天有最高的 Nestin RNA 表現,發展潛力最好,而貼 附 collagen 則保有較高的細胞量。 此外我們也利用非常高密度的細胞聚集(每平方公分達 510,000 顆),觀 察到這些細胞團塊會有方向性的聚集成更大的線狀構型,且在神經分化誘導 與沒有神經分化誘導構型上有差異。 透過免疫螢光的結果發現,聚集的細胞團,中間深色區域會有 STRO-1marker 表現,且能夠促進深色區塊外圍神經蛋白標記呈現。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:56:51Z (GMT). No. of bitstreams: 1 ntu-101-R98422015-1.pdf: 251679180 bytes, checksum: 6601847acc93844259ecf497a4df98c3 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 誌謝 .............................. ..i
中文摘要 .........................ii ABSTRACT .................. iii CONTENTS .................... v LIST OF FIGURES ......... viii LIST OF TABLES ..............xi 第一章緒論.................. 1 第二章實驗設計...............16 第三章實驗材料與方法....20 第四章實驗結果........... 28 第五章討論............ ..... 41 第六章 結論.................. 49 未來研究方向......... .... 50 參考書目................... 51 | |
| dc.language.iso | zh-TW | |
| dc.subject | 分化 | zh_TW |
| dc.subject | 聚乙烯醇 | zh_TW |
| dc.subject | 神經 | zh_TW |
| dc.subject | 牙髓幹細胞 | zh_TW |
| dc.subject | Polyvinyl-alcohol | en |
| dc.subject | DPSC | en |
| dc.subject | neurodifferentiation | en |
| dc.title | 聚乙烯醇促進牙髓幹細胞的神經分化 | zh_TW |
| dc.title | Neurodifferentiation of Dental Pulp Stem Cells Promoted by Polyvinyl-Alcohol | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 楊台鴻,章浩宏,王彥雄 | |
| dc.subject.keyword | 聚乙烯醇,牙髓幹細胞,神經,分化, | zh_TW |
| dc.subject.keyword | Polyvinyl-alcohol,DPSC,neurodifferentiation, | en |
| dc.relation.page | 98 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-13 | |
| dc.contributor.author-college | 牙醫專業學院 | zh_TW |
| dc.contributor.author-dept | 臨床牙醫學研究所 | zh_TW |
| 顯示於系所單位： | 臨床牙醫學研究所 | |
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