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http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6457完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 李昆達 | |
| dc.contributor.author | Hsiao-Han Lin | en |
| dc.contributor.author | 林筱涵 | zh_TW |
| dc.date.accessioned | 2021-05-17T09:13:58Z | - |
| dc.date.available | 2012-08-22 | |
| dc.date.available | 2021-05-17T09:13:58Z | - |
| dc.date.copyright | 2012-08-22 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-17 | |
| dc.identifier.citation | Altamura MM, Archilletti T, Capone I, Costantino P (1991) Histological analysis of the expression of Agrobacterium rhizogenes rolB-GUS gene fusions in transgenic tobacco. New Phytol 118: 69-78
Altamura MM, Capitani F, Gazza L, Capone I, Costantino P (1994) The plant oncogene rolB stimulates the formation of flower and root meristemoids in tobacco thin cell-layers. New Phytol 126: 283-293 Ark PA, Thompson JP (1961) Detection of hairy root pathogen, Agrobacterium rhizogenes, by use of fleshy roots. Phytopathology 51: 69-71 Armando Aguado-Santacruz G, Rascon-Cruz Q, Moreno-Gomez B, Gerardo Guevara-Gonzalez R, Guevara-Olvera L, Francisco Jimenez-Bremont J, Arevalo-Gallegos S, Garcia-Moya E (2009) Genetic transformation of blue grama grass with the rolA gene from Agrobacterium rhizogenes: regeneration of transgenic plants involves a 'hairy embryo' stage. In Vitro Cell Dev-Pl 45: 681-692 Banerjee S, Shang TQ, Wilson AM, Moore AL, Strand SE, Gordon MP, Doty SL (2002) Expression of functional mammalian P450 2E1 in hairy root cultures. Biotechnol Bioeng 77: 462-466 Banerjee S, Singh S, Rahman LU (2012) Biotransformation studies using hairy root cultures — A review. Biotechnol Adv 30: 461-468 Bariola PA, Howard CJ, Taylor CB, Verburg MT, Jaglan VD, Green PJ (1994) The Arabidopsis ribonuclease gene RNS1 is tightly controlled in response to phosphate limitation. Plant J 6: 673-685 Batra J, Dutta A, Singh D, Kumar S, Sen J (2004) Growth and terpenoid indole alkaloid production in Catharanthus roseus hairy root clones in relation to left- and right-termini-linked Ri T-DNA gene integration. Plant Cell Rep 23: 148-154 Bonferroni CE (1936) Teoria statistica delle classi e calcolo delle probabilit `a. Pubblicazioni del R Istituto Superiore di Scienze Economiche e Commerciali di Firenze 8: 3-62 Bulgakov VP (2008) Functions of rol genes in plant secondary metabolism. Biotechnol Adv 26: 318-324 Bulgakov VP, Khodakovskaya MV, Labetskaya NV, Chernoded GK, Zhuravlev YN (1998) The impact of plant rolC oncogene on ginsenoside production by ginseng hairy root cultures. Phytochemistry 49: 1929-1934 Capone I, Cardarelli M, Trovato M, Costantino P (1989) Upstream non-coding region which confers polar expression to Ri plasmid root inducing gene rolB. Mol Gen Genet 216: 239-244 Capone I, Frugis G, Costantino P, Cardarelli M (1994) Expression in different populations of cells of the root-meristem is controlled by different domains of the rolB promoter. Plant Mol Biol 25: 681-691 Capone I, Spano L, Cardarelli M, Bellincampi D, Petit A, Costantino P (1989) Induction and growth properties of carrot roots with different complements of Agrobacterium rhizogenes T-DNA. Plant Mol Biol 13: 43-52 Cardarelli M, Mariotti D, Pomponi M, Spano L, Capone I, Costantino P (1987) Agrobacterium rhizogenes T-DNA genes capable of inducing hairy root phenotype. Mol Gen Genet 209: 475-480 Cardarelli M, Spano L, Mariotti D, Mauro ML, Sluys MA, Costantino P (1987) The role of auxin in hairy root induction. Mol Gen Genet 208: 457-463 Carneiro M, Vilaine F (1993) Differential expression of the rolA plant oncogene and its effect on tobacco development. Plant J 3: 785-792 Chichiricco G, Costantino P, Spano L (1992) Expression of the rolB oncogene from Agrobacterium rhizogenes during zygotic embryogenesis in tobacco. Plant Cell Physiol 33: 827-832 De Smet I, Tetsumura T, De Rybel B, Frey NFd, Laplaze L, Casimiro I, Swarup R, Naudts M, Vanneste S, Audenaert D, Inze D, Bennett MJ, Beeckman T (2007) Auxin-dependent regulation of lateral root positioning in the basal meristem of Arabidopsis. Development 134: 681-690 Du Z, Zhou X, Ling Y, Zhang Z, Su Z (2010) agriGO: a GO analysis toolkit for the agricultural community. Nucleic acids Res 38: W64-70 Elmayan T, Tepfer M (1995) Evaluation in tobacco of the organ specificity and strength of the rolD promoter, domain A of the 35S promoter and the 35S2 promoter. Transgenic Res 4: 388-396 Estruch JJ, Chriqui D, Grossmann K, Schell J, Spena A (1991) The plant oncogene rolC is responsible for the release of cytokinins from glucoside conjugates. Embo J 10: 2889-2895 Estruch JJ, Parets-Soler A, Schmulling T, Spena A (1991) Cytosolic localization in transgenic plants of the RolC peptide from Agrobacterium rhizogenes. Plant Mol Biol 17: 547-550 Estruch JJ, Schell J, Spena A (1991) The protein encoded by the rolB plant oncogene hydrolyzes indole glucosides. Embo J. 10: 3125-3128 Faiss M, Strnad M, Redig P, Doležal K, Hanuš J, Van Onckelen H, Schmulling T (1996) Chemically induced expression of the rolC-encoded β-glucosidase in transgenic tobacco plants and analysis of cytokinin metabolism: rolC does not hydrolyze endogenous cytokinin glucosides in planta. Plant J 10: 33-46 Falasca G, Altamura MM, D'Angeli S, Zaghi D, Costantino P, Mauro ML (2010) The rolD oncogene promotes axillary bud and adventitious root meristems in Arabidopsis. Plant Physiol Biochem 48: 797-804 Filippini F, Loschiavo F, Terzi M, Costantino P, Trovato M (1994) The plant oncogene rolB alters binding of auxin to plant-cell membranes. Plant Cell Physiol 35: 767-771 Filippini F, Rossi V, Marin O, Trovato M, Costantino P, Mark Downey P, Lo Schiavo F, Terzi M (1996) A plant oncogene as a phosphatase. Nature 379: 499-500 Friml J, Wisniewska J, Benkova E, Mendgen K, Palme K (2002) Lateral relocation of auxin efflux regulator PIN3 mediates tropism in Arabidopsis. Nature 415: 806-809 Fujii N (1997) Pattern of DNA binding of nuclear proteins to the proximal Agrobacterium rhizogenes rolC promoter is altered during somatic embryogenesis of carrot. Gene 201: 55-62 Furmanowa M, Syklowska-Baranek K (2000) Hairy root cultures of Taxus × media var. Hicksii Rehd. as a new source of paclitaxel and 10-deacetylbaccatin III. Biotechnol Lett 22: 683-686 Garcia-Olmedo F, Molina A, Segura A, Moreno M (1995) The defensive role of nonspecific lipid-transfer proteins in plants. Trends Microbiol 3: 72-74 Gaume A, Komarnytsky S, Borisjuk N, Raskin I (2003) Rhizosecretion of recombinant proteins from plant hairy roots. Plant Cell Rep 21: 1188-1193 Guivarch A, Carneiro M, Vilaine F, Pautot V, Chriqui D (1996) Tissue-specific expression of the rolA gene mediates morphological changes in transgenic tobacco. Plant Mol Biol 30: 125-134 Hamill JD, Parr AJ, Robins RJ, Rhodes MJC (1986) Secondary product formation by cultures of Beta vulgaris and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep 5: 111-114 Hamilton AJ, Lycett GW, Grierson D (1990) Antisense gene that inhibits synthesis of the hormone ethylene in transgenic plants. Nature 346: 284-287 Hernandez-Mata G, Mellado-Rojas M, Richards-Lewis A, Lopez-Bucio J, Beltran-Pena E, Soriano-Bello E (2010) Plant immunity induced by oligogalacturonides alters root growth in a process involving flavonoid accumulation in Arabidopsis thaliana. J Plant Growth Regul 29: 441-454 Huffman GA, White FF, Gordon MP, Nester EW (1984) Hairy-root-inducing plasmid: physical map and homology to tumor-inducing plasmids. J Bacteriol 157: 269-276 Jayaraj J, Devlin R, Punja Z (2008) Metabolic engineering of novel ketocarotenoid production in carrot plants. Transgenic Res 17: 489-501 Jung HW, Kim W, Hwang BK (2003) Three pathogen-inducible genes encoding lipid transfer protein from pepper are differentially activated by pathogens, abiotic, and environmental stresses. Plant, Cell Environ 26: 915-928 Kamo KK (2003) Long-term expression of the uidA gene in gladiolus plants under control of either the ubiquitin, rolD, mannopine synthase, or cauliflower mosaic virus promoters following three seasons of dormancy. Plant Cell Rep 21: 797-803 Kim JA, Baek KH, Son YM, Son SH, Shin H (2009) Hairy root cultures of Taxus cuspidata for enhanced production of paclitaxel. J Korean Soc Appl Biol Chem 52: 144-150 Kiselev KV, Dubrovina AS, Bulgakov VP (2009) Phenylalanine ammonia-lyase and stilbene synthase gene expression in rolB transgenic cell cultures of Vitis amurensis. Appl Microbiol Biotechnol 82: 647-655 Kiselev KV, Dubrovina AS, Veselova MV, Bulgakov VP, Fedoreyev SA, Zhuravlev YN (2007) The rolB gene-induced overproduction of resveratrol in Vitis amurensis transformed cells. J Biotechnol 128: 681-692 Kruskal WH (1957) Historical notes on the Wilcoxon unpaired two-sample test. J Am Stat Assoc 52: 356-360 Kruskal WH, Wallis WA (1952) Use of ranks in one-criterion variance analysis. J Am Stat Assoc 47: 583-621 Leach F, Aoyagi K (1991) Promoter analysis of the highly expressed rolC and rolD root-inducing genes of Agrobacterium rhizogenes: enhancer and tissue-specific DNA determinants are dissociated. Plant Sci 79: 69-76 Lee KT, Chen SC, Chiang BL, Yamakawa T (2007) Heat-inducible production of beta-glucuronidase in tobacco hairy root cultures. Appl Microbiol Biotechnol 73: 1047-1053 Levesque H, Delepelaire P, Rouze P, Slightom J, Tepfer D (1988) Common evolutionary origin of the central portions of the Ri TL-DNA of Agrobacterium rhizogenes and the Ti T-DNAs of Agrobacterium tumefaciens. Plant Mol Biol 11: 731-744 Lewis DR, Negi S, Sukumar P, Muday GK (2011) Ethylene inhibits lateral root development, increases IAA transport and expression of PIN3 and PIN7 auxin efflux carriers. Development 138: 3485-3495 Magrelli A, Langenkemper K, Dehio C, Schell J, Spena A (1994) Splicing of the rolA transcript of Agrobacterium rhizogenes in Arabidopsis. Science 266: 1986-1988 Matsuki R, Uchimiya H (1994) A 43-kDa nuclear tobacco protein interacts with a specific single-stranded DNA sequence from the 5'-upstream region of the Agrobacterium rhizogenes rolC gene. Gene 141: 201-205 Maurel C, Brevet J, Barbierbrygoo H, Guern J, Tempe J (1990) Auxin regulates the promoter of the root-inducing rolB gene of Agrobacterium rhizogenes in transgenic tobacco. Mol Gen Genet 223: 58-64 Mauro ML, Trovato M, Paolis AD, Gallelli A, Costantino P, Altamura MM (1996) The plant oncogene rolD stimulates flowering in transgenic tobacco plants. Dev Biol 180: 693-700 McInnes E, Morgan AJ, Mulligan BJ, Davey MR (1991) Phenotypic effects of isolated pRiA4 TL-DNA rol genes in the presence of intact TR-DNA in transgenic plants of Solanum dulcamara L. J Exp Bot 42: 1279-1286 Negi S, Ivanchenko MG, Muday GK (2008) Ethylene regulates lateral root formation and auxin transport in Arabidopsis thaliana. Plant J 55: 175-187 Nilsson O, Crozier A, Schmulling T, Sandberg G, Olsson O (1993) Indole-3-acetic acid homeostasis in transgenic tobacco plants expressing the Agrobacterium rhizogenes rolB gene. Plant J 3: 681-689 Nilsson O, Moritz T, Imbault N, Sandberg G, Olsson O (1993) Hormonal characterization of transgenic tobacco plants expressing the rolC Gene of Agrobacterium rhizogenes TL-DNA. Plant Physiol 102: 363-371 O'Donnell PJ, Calvert C, Atzorn R, Wasternack C, Leyser HMO, Bowles DJ (1996) Ethylene as a Signal Mediating the Wound Response of Tomato Plants. Science 274: 1914-1917 Ono NN, Tian L (2011) The multiplicity of hairy root cultures: Prolific possibilities. Plant Sci 180: 439-446 Ooms G, Twell D, Bossen ME, Hoge JHC, Burrell MM (1986) Developmental regulation of Ri TL-DNA gene expression in roots, shoots and tubers of transformed potato (Solanum tuberosum cv. Desiree). Plant Mol Biol 6: 321-330 Oono Y, Satomi T, Uchimiya H (1991) Agrobacterium rhizogenes Lacz-rolC gene expression in Escherichia coli - detection of the product in transgenic plants using RolC-specific antibodies. Gene 104: 95-98 Rigden DJ, Carneiro M (1999) A structural model for the rolA protein and its interaction with DNA. Proteins: Struct, Funct, Bioinf 37: 697-708 Riker AJ, Banfield WM, Wright WH, Keitt GW, Sagen HE (1930) Studies on infectious hairy root of nursery apple trees. J Agric Res 41: 0507-0540 Schmulling T, Fladung M, Grossmann K, Schell J (1993) Hormonal content and sensitivity of transgenic tobacco and potato plants expressing single rol genes of Agrobacterium rhizogenes T-DNA. Plant J 3: 371-382 Schmulling T, Schell J, Spena A (1988) Single genes from Agrobacterium rhizogenes influence plant development. Embo J. 7: 2621-2629 Schmulling T, Schell J, Spena A (1989) Promoters of the rolA, B, and C genes of Agrobacterium rhizogenes are differentially regulated in transgenic plants. Plant Cell 1: 665-670 Slightom JL, Durand-Tardif M, Jouanin L, Tepfer D (1986) Nucleotide sequence analysis of TL-DNA of Agrobacterium rhizogenes agropine type plasmid. Identification of open reading frames. J Biol Chem 261: 108-121 Slightom JL, Jouanin L, Leach F, Drong RF, Tepfer D (1985) Isolation and identification of TL-DNA/plant junctions in Convolvulus arvensis transformed by Agrobacterium rhizogenes strain A4. Embo J. 4: 3069-3077 Spano L, Wullems GJ, Schilperoort RA, Costantino P (1981) Hairy root - in vitro growth properties of tissues induced by Agrobacterium rhizogenes on tobacco. Plant Sci Lett 23: 299-305 Spena A, Estruch JJ, Prinsen E, Nacken W, Vanonckelen H, Sommer H (1992) Anther-specific expression of the rolB Gene of Agrobacterium rhizogenes increases IAA content in anthers and alters anther development and whole flower growth. Theor Appl Genet 84: 520-527 Sugaya S, Hayakawa K, Handa T, Uchimiya H (1989) Cell-specific expression of the rolC gene of the TL-DNA of Ri plasmid in transgenic tobacco plants. Plant Cell Physiol 3: 649-654 Sugaya S, Uchimiya H (1992) Deletion analysis of the 5'-upstream region of the Agrobacterium rhizogenes Ri plasmid rolC gene required for tissue-specific expression. Plant Physiol 99: 464-467 Sun L-Y, Monneuse M-O, Martin-Tanguy J, Tepfer D (1991) Changes in flowering and the accumulation of polyamines and hydroxycinnamic acid-polyamine conjugates in tobacco plants transformed by the rolA locus from the Ri TL-DNA of Agrobacterium rhizogenes. Plant Sci 80: 145-156 Suzuki A, Kato A, Uchimiya H (1992) Single-stranded DNA of 5'-upstream region of the rolC gene interacts with nuclear proteins of carrot cell cultures. Biochem Biophys Res Commun 188: 727-733 Swain SS, Sahu L, Barik DP, Chand PK (2010) Agrobacterium × plant factors influencing transformation of `Joseph's coat' (Amaranthus tricolor L.). Sci Hortic-Amsterdam 125: 461-468 Tamot BK, Pauls KP, Glick BR (2003) Root and hypocotyl growth in transgenic tomatoes that express the bacterial enzyme ACC deaminase. J Plant Biol 46: 181-186 Taylor BH, White FF, Nester EW, Gordon MP (1985) Transcription of Agrobacterium rhizogenes A4 T-DNA. Mol Gen Genet 201: 546-553 Team RDC (2011) R: A language and environment for statistical computing. Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol Prog 9: 12-20 Trovato M, Maras B, Linhares F, Costantino P (2001) The plant oncogene rolD encodes a functional ornithine cyclodeaminase. Proc Natl Acad Sci USA 98: 13449-13453 Van Altvorst AC, Bino RJ, Van Dijk AJ, Lamers AMJ, Lindhout WH, Van Der Mark F, Dons JJM (1992) Effects of the introduction of Agrobacterium rhizogenes rol genes on tomato plant and flower development. Plant Sci (Shannon) 83: 77-85 Vilaine F, Cassedelbart F (1987) Independent induction of transformed roots by the TL and TR regions of the Ri plasmid of agropine type Agrobacterium rhizogenes. Mol Gen Genet 206: 17-23 Vilaine F, Cassedelbart F (1987) A new vector derived from Agrobacterium rhizogenes plasmids: a micro-Ri plasmid and its use to construct a mini-Ri plasmid. Gene 55: 105-114 Vilaine F, Rembur J, Chriqui D, Tepfer M (1998) Modified development in transgenic tobacco plants expressing a rolA∷GUS translational fusion and subcellular localization of the fusion protein. Mol Plant Microbe In 11: 855-859 Welander M, Zhu L-H (2010) Rol Genes: Molecular Biology, Physiology, Morphology, Breeding Uses. In Plant Breeding Reviews. John Wiley & Sons, Inc., pp 79-103 White FF, Ghidossi G, Gordon MP, Nester EW (1982) Tumor induction by Agrobacterium rhizogenes involves the transfer of plasmid DNA to the plant genome. Proc Natl Acad Sci USA 79: 3193-3197 White FF, Nester EW (1980) Hairy root: plasmid encodes virulence traits in Agrobacterium rhizogenes. J Bacteriol 141: 1134-1141 White FF, Taylor BH, Huffman GA, Gordon MP, Nester EW (1985) Molecular and genetic analysis of the transferred DNA regions of the root-inducing plasmid of Agrobacterium rhizogenes. J Bacteriol 164: 33-44 Xue Z-T, Holefors A, Welander M (2008) Intron splicing in 5' untranslated region of the rolA transcript in transgenic apple. J Plant Physiol 165: 544-552 Yokoyama R, Hirose T, Fujii N, Aspuria ET, Kato A, Uchimiya H (1994) The rolC promoter of Agrobacterium rhizogenes Ri plasmid is activated by sucrose in transgenic tobacco plants. Mol Gen Genet 244: 15-22 Zhou J, Lee C, Zhong R, Ye Z-H (2009) MYB58 and MYB63 Are Transcriptional Activators of the Lignin Biosynthetic Pathway during Secondary Cell Wall Formation in Arabidopsis. Plant Cell 21: 248-266 | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6457 | - |
| dc.description.abstract | 毛狀根 (hairy root) 是根毛農桿菌 (Agrobacterium rhizogenes) 感染植物後誘發生成的癌化組織，此組織被視為是最有潛力的植物次級代謝物生產工廠。文獻指出根毛農桿菌 Ri 質體上的 rol 基因群 (rolA 、 rolB 、 rolC 以及 rolD) 會影響毛狀根的生成。然而，尚未有文獻報導 rol 基因群如何影響毛狀根形態，以及這些基因影響的植物生理機能。因此，本篇研究之動機在利用形態調查及轉錄體學來比較野生型及個別 rol 基因缺陷之根毛農桿菌感染之菸草毛狀根之差異。形態調查結果顯示， rolB / rolC 根毛農桿菌缺陷株在毛狀根誘導率及外表型上與野生型有顯著差異，而 rolA / rolD 的影響不大。轉錄體分析 rolB / rolC 缺陷毛狀根與野生型毛狀根的結果顯示： RolB 蛋白質可能對於菸草毛狀根的脂質運送、細胞胺基酸相關生合成路徑、創傷反應、以及乙烯刺激反應具有正向調控功能； RolC 蛋白質則對於創傷反應、化學刺激反應、醣類代謝、以及脂質運送具有正向調控功能。上述結果揭示了 rolB / rolC 如何影響毛狀根形態，以及它們在毛狀根組織中調控的生理機能。 | zh_TW |
| dc.description.abstract | Hairy root is the tumorized tissue induced when Agrobacterium rhizogenes infects plants, and is considered as the most powerful plant secondary metabolites-producing system. The formation of hairy root have been known to related to four root inducing (rol) genes rolA, rolB, rolC, and rolD that lie in the transferred DNA (T-DNA) of A. rhizogenes root inducing (Ri) plasmid. However, no studies showed how these rol genes affect hairy root architecture. Also, there is no comprehensive –omics information of how plant cell is manipulated by the rol genes. Therefore, this study tried to answer these questions by morphological and transcriptomic analysis of tobacco hairy root induced by wild type and rol deletion mutant of A. rhizogenes. Morphological analysis revealed that lacking rolB and rolC cause impaired hairy root syndrome, while lacking rolA and rolD had little or no effects. Transcriptomic comparison of rolB / rolC deleted hairy roots compared with that of wild type indicate that RolB may positively regulate lipid transport, cellular amino acid derivative biosynthesis process, response to wounding, and response to ethylene stimulus. Meanwhile, the biological processes that may be positively regulated by RolC are response to wounding, response to chemical stimulus, carbohydrate metabolic process, and lipid transport. The results revealed how rolB and rolC affect hairy root morphology and what are the corresponding biological processes they manipulated. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-17T09:13:58Z (GMT). No. of bitstreams: 1 ntu-101-R99b22007-1.pdf: 8208424 bytes, checksum: 0cefee0a862c8500d488458d9ca26980 (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 口試委員會審定書 i
謝誌 ii 摘要 iii Abstract iv Chapter 1. Introduction 5 1.1 Aim of research 5 1.2 General backgrounds of hairy root 5 1.3 RolA 8 1.4 RolB 10 1.5 RolC 12 1.6 RolD 14 1.7 Research strategies 15 Chapter 2. Materials and Methods 17 2.1 Plant materials and growth conditions 17 2.2 Bacteria and growth conditions 17 2.3 Genotype confirmation for A. rhizogenes strain A4 derivatives 18 2.4 Hairy root induction 18 2.5 Hairy root initiation test 19 2.6 Hairy root genotype confirmation 20 2.7 Analysis of hairy root architectures 20 2.8 Statistical analysis 21 2.9 RNA extraction 22 2.10 Microarray labeling, scanning, and data extracting 23 2.11 Gene ontology of microarray data 23 2.12 qRT-PCR 24 Chapter 3. Results 26 3.1 Genotype confirmation of rol gene-deficient A. rhizogenes strain A4 26 3.2 Tobacco infection by A. rhizogenes strain A4 and its derivatives 26 3.3 Confirmation of hairy root genotype 27 3.4 Hairy root initiation test 28 3.5 Measurements of hairy root architecture 29 3.6 Microarray Data Analysis 32 3.7 Gene ontology of HR (delta)rolB down-regulated genes 33 3.8 Gene Ontology of HR (delta)rolC down-regulated genes 33 3.9 qRT-PCR Analysis of the Gene Ontology-extracted genes 34 Chapter 4. Discussions 36 4.1 rolB and rolC play crucial roles in hairy root initiation 36 4.2 rolB and rolC affect hairy root architecture more drastically 36 4.3 Difficult maintenance of the HR (delta)rolB and HR (delta)rolC clones 37 4.4 Comparison between the microarray and qRT-PCR analyses 38 4.5 Biological processes manipulated in the absence of rolB 38 4.6 Biological processes manipulated in the absence of rolC 40 4.7 Deduced biological functions of rolB 41 4.8 Deduced biological functions of rolC 42 Chapter 5. Conclusion 44 Tables 45 Figures 63 References 84 Appendix 91 | |
| dc.language.iso | en | |
| dc.subject | 轉錄體分析 | zh_TW |
| dc.subject | 毛狀根 | zh_TW |
| dc.subject | rol 基因群 | zh_TW |
| dc.subject | 根毛農桿菌 | zh_TW |
| dc.subject | 菸草 | zh_TW |
| dc.subject | A. rhizogenes | en |
| dc.subject | hairy root | en |
| dc.subject | transcriptomic analysis | en |
| dc.subject | N. tabacum | en |
| dc.subject | rol genes | en |
| dc.title | 從菸草毛狀根之型態與轉錄體探討根毛農桿菌 rol 基因之功能 | zh_TW |
| dc.title | Functional exploration of Agrobacterium rhizogenes rol genes by morphological and transcriptomic analyses of tobacco hairy roots | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 劉啟德,劉力瑜,常怡雍,鄭秋萍,楊健志 | |
| dc.subject.keyword | 毛狀根,rol 基因群,根毛農桿菌,菸草,轉錄體分析, | zh_TW |
| dc.subject.keyword | hairy root,rol genes,A. rhizogenes,N. tabacum,transcriptomic analysis, | en |
| dc.relation.page | 92 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2012-08-18 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 生化科技學系 | zh_TW |
| 顯示於系所單位： | 生化科技學系 | |
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