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標題: | 水稻蔗糖轉運蛋白基因OsSUT4受刻傷誘導表現之調控機制 Regulation of mechanical wounding induced expression of sucrose transporter gene OsSUT4 |
作者: | Nai-Chiang Dai 戴乃強 |
指導教授: | 王淑珍(Shu-Jen Wang) |
關鍵字: | 機械傷害,水稻,蔗糖轉運蛋白,啟動子, Mechanical wounding,promoter,rice (Oryza sativa L.),sucrose transporter, |
出版年 : | 2013 |
學位: | 碩士 |
摘要: | 植物生長過程中會遭受到許多的生物和非生物造成之刻傷逆境。當植物遇刻傷逆境時將會調整其生理及代謝反應以進行修補及防禦反應。糖類分子於刻傷逆境反應中具有提供修補所需碳源及能量之功能。水稻蔗糖轉運蛋白(sucrose transporter, SUT)家族基因中之OsSUT4的表現量會受到機械性傷害和斜紋夜盜蟲(Spodoptera litura)的咬食之刺激而明顯增強。在機械性傷害處理之葉部,與蔗糖代謝相關之蔗糖水解酵素(OsCIN1)及單糖轉運蛋白(OsMST6)之表現也會受到刻傷逆境誘導。然而當水稻莖稈受到褐飛蝨(Nilaparvata lugens)刺吸後OsSUT4的基因表現卻會受到抑制。進一步之試驗結果顯示,機械性刻傷處理後,茉莉酸(JA)和乙烯生合成相關基因均有增加之現象,且此二種荷爾蒙均可促進OsSUT4基因表現,另以茉莉酸合成抑制劑處理水稻植株將會降低機械性刻傷誘導OsSUT4基因表現之反應。此外,刻傷處理將誘導H2O2累積,且H2O2具增強刻傷刺激誘導OsSUT4基因表現之功能。進而,藉由OsSUT4之不同長度的啟動子接上GUS報導基因之水稻轉殖株分析,發現調控OsSUT4受機械性刻傷誘導表現之啟動子調控序列位於轉譯起始點至其上游248個核苷酸序列間。 Plants are often suffered by wounding stresses caused by biotic and abiotic factors during growth and development, and they will modulate physiological and metabolic behavior to process repair and defense responses. Sugars play an important role to provide carbon and energy source for healing at wound site. The expression of OsSUT4 gene, one of rice sucrose transporter family member, was significantly enhanced by mechanical woundsing and Spodoptera litura chewing stimuli. However, when rice plants was attacked by sap-sucking pest, i.e. Nilaparvata lugens, OsSUT4 gene expression in rice culm was down-regulated. In addition to OsSUT4 expression, the transcript levels of the cell wall invertase gene (OsCIN1) and monosaccharide transporter gene (OsMST6) were also increased in mechanical wounding-treated rice plants. Expression of OsSUT4 can be promoted by exogenous jasmonate (JA) and ethylene. Furthermore, if JA biosynthesis pathway was blocked, the wound-induced OsSUT4 expression would be repressed. In addition, since NADPH oxidase inhibitor treatment can reduce the wounding effect on OsSUT4 expression, H2O2 was considered to function as a signal factor involved to the regulatory pathway of wound-regulated OsSUT4 expression. According to OsSUT4 promoter activity analysis in transgenic rice plants, it was suggested that the wounding-responsive element on OsSUT4 promoter was located at the region within 248 bp upstream of the translation start codon. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6447 |
全文授權: | 同意授權(全球公開) |
顯示於系所單位: | 農藝學系 |
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