請用此 Handle URI 來引用此文件：
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6439完整後設資料紀錄
| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 張繼堯(Chi-Yao Chang) | |
| dc.contributor.author | Chien-Wei Liu | en |
| dc.contributor.author | 劉千維 | zh_TW |
| dc.date.accessioned | 2021-05-16T16:29:35Z | - |
| dc.date.available | 2014-08-23 | |
| dc.date.available | 2021-05-16T16:29:35Z | - |
| dc.date.copyright | 2013-08-23 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-08-19 | |
| dc.identifier.citation | 劉文御 (2003)。養殖水產生物病害防治。行政院農業委員會水產試驗所。
冉繁華 (2007)。 石斑Handbook of Grouper。台灣漁業經濟發展協會。 張文重 (2009)。水產種苗培育技術。前程出版社。 行政院農業委員會漁業署 (2012) 2011年漁業年報。高雄市:行政院農業委員會漁業署，p. 37。 林姵妏 (2010)。石斑魚虹彩病毒極早期基因078R和030L之功能分析。國立台灣海洋大學生物科技研究所博士學位論文。 楊政霖 (2012)。點帶石斑魚CD9 基因之選殖、特性鑑定及感染虹彩病毒之表現分析。國立台灣大學生命科學院漁業科學所碩士論文。 Aroor, A. R., McKarns, S., Demarco, V. G., Jia, G. and Sowers, J. R. (2013). Maladaptive immune and inflammatory pathways lead to cardiovascular insulin resistance. Metabolism. pii: S0026-0495(13)00209-6. Boucheix, C. and Rubinstein, E. (2001). Tetraspanins. Cell. Mol. Life Sci. 58, 1189-1205. Cavalli, L. R., Riggins, R. B., Wang, A., Clarke, R., Haddad, B. R. (2010). Frequent loss of heterozygosity at the interferon regulatory factor-1 gene locus in breast cancer. Breast Cancer Res. Treat.121(1), 227–231. de Knegt, R. J., and van den Berg, A. P. (2001). Hepatitis C virus infection. N. Engl. J. Med. 345, 1427; author reply 1427-1428. de Parseval, A., Lerner, D. L., Borrow, P., Willett, B. J., and Elder, J. H. (1997). Blocking of feline immunodeficiency virus infection by a monoclonal antibody to CD9 is via inhibition of virus release rather than interference with receptor binding. J. Virol . 71, 5742-5749. Do Carmo, S., Seguin, D., Milne, R. and Rassart, E. (2002). Modulation of Apolipoprotein D and Apolipoprotein E mRNA Expression by Growth Arrest and Identification of Key Elements in the Promoter. J. Biol. Chem. 277(7), 5514–5523. Fukudome, K., Furuse, M., Imai, T., Nishimura, M., Takagi, S., Hinuma, Y., and Yoshie, O. (1992). Identification of membrane antigen C33 recognized by monoclonal antibodies inhibitory to human T-cell leukemia virus type 1 (HTLV-1)-induced syncytium formation: altered glycosylation of C33 antigen in HTLV-1-positive T cells. J. Virol. 66, 1394-1401. Gao, J., Senthil, M., Ren, B., Yan, J., Xing, Q., Yu, J., Zhang, L., and Yim, J. H. (2010). IRF-1 transcriptionally up-regulates PUMA which mediates the mitochondrial apoptotic pathway in IRF-1 induced apoptosis in cancer cells. Cell Death Differ. 17(4), 699-709. Garcia, E., Nikolic, D. S. and Piguet, V. (2008). HIV-1 replication in dendritic cells occurs through a tetraspanin-containing compartment enriched in AP-3. Traffic. 9(2), 200-214. Gluschankof, P., Mondor, I., Gelderblom, H. R., and Sattentau, Q. J. (1997). Cell membrane vesicles are a major contaminant of gradient-enriched human immunodeficiency virus type-1 preparations. Virology. 230, 125-133. Hemler, M. E. (2003). Tetraspanin proteins mediate cellular penetration, invasion, and fusion events and define a novel type of membrane microdomain. Annu. Rev. Cell Dev. Biol. 19, 397-422. Hemler, M. E. (2005). Tetraspanin functions and associated microdomains. Nat. Rev. Mol. Cell Biol. 6, 801-811. Huang, Y., Walstrom, A., Zhang, L., Zhao, Y., Cui, M., Ye, L., Zheng, J. C. (2009). Type I Interferons and Interferon Regulatory Factors Regulate TNF-Related Apoptosis-Inducing Ligand (TRAIL) in HIV-1-Infected Macrophages. PLoS ONE. 4(4), e5397. Kaji, K., Oda, S., Shikano, T., Ohnuki, T., Uematsu, Y., Sakagami, J., Tada, N., Miyazaki, S. and Kudo, A. (2000) The gamete fusion process is defective in eggs of CD9-deficient mice. Nat. Genet. 24, 279–282. Khurana, S., Krementsov, D. N., de Parseval, A., Elder, J. H., Foti, M. and Thali, M. (2007). Human immunodeficiency virus type 1 and influenza virus exit via different membrane microdomains. J. Virol. , 81(22), 12630-12640. Kida, Y., Inoue, H., Shimizu, T. and Kuwano, K. (2007). Serratia marcescens serralysin induces inflammatory responses through protease-activated receptor 2. Infect. Immun. 75(1): 164–174. Krementsov, D. N., Weng, J., Lambele, M., Roy, N. H. and Thali, M. (2009). Tetraspanins regulate cell-to-cell transmission of HIV-1. Retrovirology . 6, 64. Krementsov, D. N., Rassam, P., Margeat, E., Roy, N. H., Schneider-Schaulies, J., Milhiet, P. E. and Thali, M. (2010). HIV-1 assembly differentially alters dynamics and partitioning of tetraspanins and raft components. Traffic. 11(11), 1401-1414. Kurzeder, C., Koppold, B., Sauer, G., Pabst, S., Kreienberg, R. and Deissler, H. (2007). CD9 promotes adeno-associated virus type 2 infection of mammary carcinoma cells with low cell surface expression of heparan sulphate proteoglycans. Int. J. Mol. Med. 19(2), 325-333. Le Naour, F., Rubinstein, E., Jasmin, C., Prenant, M. and Boucheix, C. (2000). Severely reduced female fertility in CD9-deficient mice. Science. 287, 319–321. Loffler, S., Lottspeich, F., Lanza, F., Azorsa, D. O., ter Meulen, V. and Schneider-Schaulies, J. (1997). CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71(1), 42-49. Mamane, Y., Heylbroeck, C., Genin, P., Algarte, M., Servant, M. J., LePage, C., DeLuca, C., Kwon, H., Lin, R., Hiscott, J. (1999). Interferon regulatory factors: the next generation. Gene. 237, 1-14. Martin, F., Roth, D. M., Jans, D. A., Pouton, C. W., Partridge, L. J., Monk, P. N., and Moseley, G. W. (2005). Tetraspanins in viral infections: a fundamental role in viral biology. J. Virol. 79, 10839-10851. Meerloo, T., Sheikh, M. A., Bloem, A. C., de Ronde, A., Schutten, M., van Els, C. A., Roholl, P. J., Joling, P., Goudsmit, J. and Schuurman, H. J. (1993). Host cell membrane proteins on human immunodeficiency virus type 1 after in vitro infection of H9 cells and blood mononuclear cells. An immuno-electron microscopic study. J. Gen. Virol. 74 Pt 1), 129-135. Miyado, K., Yamada, G., Yamada, S., Hasuwa, H., Nakamura, Y., Ryu, F., Suzuki, K., Kosai, K., Inoue, K., Ogura, A., Okabe, M. and Mekada E. (2000). Requirement of CD9 on the egg plasma membrane for fertilization. Science. 287, 321-324. Monk, P. N., Partridge, L. J. (2012). Tetraspanins-gateways for Infection. Infect. Disord. Drug Targets. 12(1), 4-17. Napetschnig, J. and Wu, H. (2013). Molecular Basis of NF-κB Signaling. Annu Rev Biophys. 42, 443-68. Nydegger, S., Khurana, S., Krementsov, D. N., Foti, M. and Thali, M. (2006). Mapping of tetraspanin-enriched microdomains that can function as gateways for HIV-1. J. Cell Biol. 173(5), 795-807. Penas, P. F., Garcia-Diez, A., Sanchez-Madrid, F., Yanez-Mo, M. (2000). Tetraspanins are localized at motility-related structures and involved in normal human keratinocyte wound healing migration. J. Invest. Dermatol. 114(6), 1126-1135. Pileri, P., Uematsu, Y., Campagnoli, S., Galli, G., Falugi, F., Petracca, R., Weiner, A.J., Houghton, M., Rosa, D., Grandi, G. and Abrignani, S. (1998). Binding of hepatitis C virus to CD81. Science. 282(5390), 938-941. Rudnicka, D., Feldmann, J., Porrot, F., Wietgrefe, S., Guadagnini, S., Prevost, M. C., Estaquier, J., Haase, A. T., Sol-Foulon, N. and Schwartz, O. (2009). Simultaneous cell-to-cell transmission of human immunodeficiency virus to multiple targets through polysynapses. J. Virol. 83(12), 6234-6246. Saha, B., Jyothi Prasanna, S., Chandrasekar, B. and Nandi, D. (2010). Gene modulation and immunoregulatory roles of Interferonγ. Cytokine. 50, 1–14. Schmid, E., Zurbriggen, A., Gassen, U., Rima, B., ter Meulen, V., Schneider-Schaulies, J. (2000). Antibodies to CD9, a tetraspan transmembrane protein, inhibit canine distemper virus-induced cell-cell fusion but not virus-cell fusion. J. Virol. 74(16), 7554-7561. Seigneuret, M., Delaguillaumie, A., Lagaudriere-Gesbert, C., and Conjeaud, H. (2001). Structure of the tetraspanin main extracellular domain. A partially conserved fold with a structurally variable domain insertion. J. Biol. Chem. 276(43), 40055-40064. Shaw, M. L., Stone, K. L., Colangelo, C. M., Gulcicek, E. E. and Palese, P. (2008). Cellular proteins in influenza virus particles. PLoS Pathog. 4(6), e1000085. Stipp, C.S., Kolesnikova, T.V., and Hemler, M.E. (2003). Functional domains in tetraspanin proteins. Trends Biochem. Sci. 28, 106-112. Taniguchi, T., Ogasawara, K., Takaoka, A. and Tanaka, N. (2001). IRF family of transcription factors as regulators of host defense. Annu. Rev. Immunol. 19, 623–655. von Lindern, J. J., Rojo, D., Grovit-Ferbas, K., Yeramian, C., Deng, C., Herbein, G., Ferguson, M. R., Pappas, T. C., Decker, J. M., Singh, A., Collman, R. G. and O'Brien, W. A. (2003). Potential role for CD63 in CCR5-mediated human immunodeficiency virus type 1 infection of macrophages. J. Virol. 77(6), 3624-3633. Wang, H., Iakova, P., Wilde, M., Welm, A., Goode, T., Roesler, W. J. and Timchenko, N. A. (2001). C/EBPalpha arrests cell proliferation through direct inhibition of Cdk2 and Cdk4. Mol. Cell. 8(4), 817–828. Wiley, R. D. and Gummuluru, S. (2006). Immature dendritic cell-derived exosomes can mediate HIV-1 trans infection. Proc. Natl. Acad. Sci.U S A. 103(3), 738-743. (a) Willett, B., Hosie, M., Shaw, A., Neil, J. (1997). Inhibition of feline immunodeficiency virus infection by CD9 antibody operates after virus entry and is independent of virus tropism. J. Gen. Virol. 78( Pt 3), 611-618. (b) Willett, B. J., Hosie, M. J., Jarrett, O. and Neil, J. C. (1994). Identification of a putative cellular receptor for feline immunodeficiency virus as the feline homologue of CD9. Immunology. 81 (2), 228-33; (c) Willett, B. J. and Neil, J. C (1995). cDNA cloning and eukaryotic expression of feline CD9. Mol. Immunol. 32(6), 417-423. Wu, M. S., Chen, C. W., Liu, Y. C., Huang, H. H., Lin, C. H., Tzeng, C. S. and Chang, C. Y. (2012). Transcriptional analysis of orange-spotted grouper reacting to experimental grouper iridovirus infection. Dev. Comp. Immunol. 37 (2), 233-42. Yao, Y. L. and Yang, W. M. (2003). The Metastasis-associated Proteins 1 and 2 Form Distinct Protein Complexes with Histone Deacetylase Activity. J. Biol. Chem. 278(43), 42560-8. Yanez-Mo, M., Barreiro, O., Gordon-Alonso, M., Sala-Valdes, M., Sanchez-Madrid, F. (2009). Tetraspanin-enriched microdomains: a functional unit in cell plasma membranes. Trends Cell Biol. 19(9):434-46. Yu, K. P., Heston, L., Park, R., Ding, Z., Wang'ondu, R., Delecluse, H. J., Miller, G. (2013). Latency of Epstein-Barr virus is disrupted by gain-of-function mutant cellular AP-1 proteins that preferentially bind methylated DNA. Proc. Natl. Acad. Sci. U S A. 110(20), 8176-81. Zhang S., Kodys K., Babcock G. J, and Szabo1 G. (2012) CD81/CD9 Tetraspanins Aid Plasmacytoid Dendritic Cells in Recognition of Hepatitis C Virus–Infected Cells and Induction of Interferon-Alpha. Hepatology. 000, 000. Zhu, G. Z., Miller, B. J., Boucheix, C., Rubinstein, E., Liu, C. C., Hynes, R. O., Myles, D. G. and Primakoff, P. (2002). Residues SFQ (173–175) in the large extracellular loop of CD9 are required for gamete fusion. Development. 129, 1995-2002. Zoller, M. (2009). Tetraspanins: push and pull in suppressing and promoting metastasis. Nat Rev Cancer. 9, 40-55. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6439 | - |
| dc.description.abstract | 石斑魚是台灣水產養殖重要魚種，具高經濟價值，然其幼苗成長時期易受石斑魚虹彩病毒 (grouper Iridovirus, GIV) 感染而大量死亡，故本實驗室以易於養成之點帶石斑魚 (Epinephelus coioides) 為材料，研究石斑魚與GIV之交互作用。2012年吳與劉於本實驗室對點帶石斑魚施打poly(I:C)、GIV及LPS，發現poly(I:C) 及GIV注射後，CD9表現模式與其他干擾素 (interferon, IFN) 相關之基因相仿。IFN在病毒感染後，扮演先天性免疫與後天性免疫間重要的橋樑，影響抗病反應，但CD9的角色是個謎，2012年楊取得點帶石斑魚CD9 cDNA全長，並發現主要表現在免疫器官上，故希望取得CD9啟動子以了解與GIV之關係。
根據本實驗室所獲得之點帶石斑魚CD9 cDNA全長，利用染色體步移技術 (Genome Walking)，獲得約1.2 Kb及2.6 Kb的CD9啟動子 (promoter)，經定序並分析其轉錄因子結合位 (transcription factor binding site) 後，預測CD9啟動子可能含有AP-1、ApoD、C/EBP、NF-κB、TNF-α-Y-BOX、IRF1和IRF2的結合位。透過冷光酵素的測定 (Luciferase Assay) ，得知在GK (grouper kidney) 細胞及GB (grouper brain) 細胞，1.2 Kb CD9 有一定量表現，2.6 Kb CD9近乎不表現，且活性皆不受不同濃度poly(I:C) 的影響。藉由GK細胞中CD9啟動子的刪除實驗得知，在1.2 Kb及2.6 Kb 間的CD9啟動子可能含有抑制調控因子的結合位；1.2 Kb CD9在相對靠近轉錄起點端的124 bp及400 bp至679 bp之間可能有關鍵影響CD9表現之結合位。不同大小的CD9啟動子，在GK細胞均不受不同濃度poly(I:C) 的影響，感染GIV後，可能受抑制而近乎不表現CD9。透過NF-κB之次單元c-rel於GK細胞的共同轉染，可促進CD9啟動子的調控。以RT-PCR測定之內生性CD9在GK細胞不同濃度poly(I:C)下不同時間點的表現均無差異。自點帶石斑魚活體的頭腎 (Head Kidney, HK) 及脾臟 (Spleen) 組織純化出的白血球細胞，添加poly(I:C) 後，以RT-PCR測定，發現CD9在HK及Spleen白血球細胞不同的時間點均沒有差異，然而頭腎及脾臟白血球細胞中的IFNa與Mx都會受到poly(I:C) 的刺激而提高表現量，並在達到高峰後降低表現。 綜合本研究結果，認為CD9基因的調控可能與NF-κB有關，且可能受到GIV感染而受到抑制，然而CD9的表現是否還可能與其他因子有關及在GIV感染時扮演什麼樣的角色有待進一步證實。 | zh_TW |
| dc.description.abstract | High economic benefits make groupers important aquacultural species in Taiwan. However, the disease caused by grouper iridovirus (GIV) has resulted in economic losses due to high mortality in grouper culture. Therefore we take Epinephelus coioides which easily raised as our model to study the interactions between groupers and GIV. In 2012, Liu and Wu injected poly(I:C), GIV and LPS into orange-spotted groupers and discovered the expression pattern of CD9 resembling to those genes associated with interferon (IFN). IFN influencing antiviral effects are important bridges between innate immunity and adaptive immunity, but the role of CD9 remains unknown. We gained the total length of CD9 cDNA from orange-spotted grouper (Yang, 2012), finding that CD9 mainly expressed in immune organs. Thus, we hope to get CD9 promoter for further analysis to realize its relationship to GIV. According to the total length of CD9 cDNA gained, we obtained 1.2 Kb and 2.6 Kb CD9 promoters from genome walking. Through analysis of sequence and transcription factor binding sites, we predicted that CD9 promoters might contain binding sites of AP-1, ApoD, C/EBP, NF-κB, TNF-α-Y-BOX, IRF1 and IRF2. By luciferase assay, we found that 1.2 Kb CD9 prmoter but not 2.6 Kb CD9 promoter regularly expressed in
GK cells and GB cells, and their activities weren't affected by different concentration of poly(I:C). In deletion analysis of CD9 promoter in GK cells, 2.6 Kb CD9 promoter might have negative regulatory factor binding sites. Some binding sites located in 124 bp near the side close to transcription initiation point and the part between 400 bp and 679 bp of CD9 promoter might play a key role to affect expression. CD9 promoters with different lengths were also not affected by various concentration of poly(I:C), but the infection of GIV inhibit CD9 promoters, causing extremely low activity. Cotransfection with subunit of NF-κB, c-rel in GK cells up-regulated CD9 promoter. Measuring endogenious CD9 expressions in response to poly(I:C) treatment in GK cells by RT-PCR showed no differences. Although the percoll purified white blood cells from head kidney and spleen of orange-spotted grouper showed no CD9 transcriptional difference after poly(I:C) treatment, IFNa and Mx expression showed significant upregulation. In summary, the CD9 transcriptional regulation might associate with NF-κB, and might be inhibited by the infection of GIV. However, the transcription factors involving in CD9 promoter regulation during GIV infection remain further study. | en |
| dc.description.provenance | Made available in DSpace on 2021-05-16T16:29:35Z (GMT). No. of bitstreams: 1 ntu-102-R00b45020-1.pdf: 20988310 bytes, checksum: 5b982790fd85023de54d855ad376e9af (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 謝辭............................................................................................. I
摘要............................................................................................. II Abstract....................................................................................... III 目錄.............................................................................................. V 圖目錄...........................................................................................VIII 表目錄 ......................................................................................... IX 附錄.............................................................................................. X 第一章、前言................................................................................. 1 1.1 石斑魚簡介與台灣養殖概況........................................................ 1 1.2 虹彩病毒(Iridovirus) ................................................................ 3 1.2.1 虹彩病毒分類......................................................................... 3 1.2.2 石斑魚虹彩病毒與魚類的關係................................................... 3 1.3 四穿膜蛋白家族(tetraspanin superfamily) ................................. 4 1.3.1 四穿膜蛋白家族在生物體中扮演的角色...................................... 4 1.3.2 四穿膜蛋白家族的結構特徵...................................................... 5 1.3.3 四穿膜蛋白網絡結構............................................................... 5 1.3.4 四穿膜蛋白與病毒之間的交互關係............................................ 5 1.4 CD9 (Cluster of Differentiation 9) ........................................... 6 1.4.1 CD9...................................................................................... 6 1.4.2 CD9 與病毒之間的交互關係..................................................... 6 1.5 研究動機.................................................................................. 7 第二章、材料與方法......................................................................... 8 實驗材料與藥品試劑......................................................................... 8 實驗方法........................................................................................10 2.1 細胞培養 (cell culture) .............................................................10 2.1.1 細胞株及培養條件..................................................................10 2.1.2 細胞繼代 (passage) 培養........................................................10 2.2實驗魚隻...................................................................................10 2.3 Genomic DNA純化...................................................................10 2.4 染色體步移 (genome walking) ..................................................11 2.5冷光酵素報導表現載體 (luciferase reporter vector)之構築.............11 2.6 啟動子序列刪除分析...................................................................11 2.7 轉形作用...................................................................................12 2.8轉染作用................................................................................... 12 2.9 冷光酵素測定 ( luciferase assay)................................................ 12 2.10 石斑魚虹彩病毒效價(titer)測定.................................................. 13 2.11多層次Percoll濃度差分離............................................................13 2.12 RNA萃取.................................................................................13 2.13 RT-PCR..................................................................................14 第三章、結果...................................................................................15 3.1 點帶石斑魚CD9啟動子選殖與轉錄因子結合位分析..........................15 3.2點帶石斑魚CD9啟動子的活性分析.................................................15 3.2.1 Poly(I:C) 對點帶石斑魚CD9啟動子的活性分析.............................15 3.2.2 點帶石斑魚CD9啟動子刪除分析.................................................16 3.2.2.1 點帶石斑魚不同長度之CD9啟動子分析....................................16 3.2.2.2不同濃度poly(I:C) 對GK細胞中點帶石斑魚不同長度CD9 啟動子的影響...................................................................................16 3.2.2.3 GIV的感染對在GK細胞中不同長度的點帶石斑魚D9啟 動子活性影響...................................................................................17 3.2.2.4 共同轉染石斑魚c-rel基因對點帶石斑魚CD9啟動子活性 的影響............................................................................................17 3.3 RT-PCR....................................................................................18 3.3.1 Poly(I:C) 處理不同時間後之點帶石斑魚內生性CD9於GK細胞 的表現分析......................................................................................18 3.3.2 Poly(I:C) 處理點帶石斑魚HK和Spleen白血球細胞對內生性 CD9、IFN及Mx基因在不同時間點的表現影響......................................18 第四章、討論.................................................................................. 20 參考文獻........................................................................................ 24 | |
| dc.language.iso | zh-TW | |
| dc.title | 點帶石斑魚 CD9 基因啟動子之活性與特性及添加 poly(I:C) 與感染石斑魚虹彩病毒的表現分析 | zh_TW |
| dc.title | Activity and Characterization of Orange-spotted Grouper (Epinephelus coioides) CD9 Promoter, and Its Expression Analysis in Response to Poly(I:C) and GIV Infection. | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 蘇建國(Jyan-Gwo J. Su),林正輝(Cheng-Hui Lin) | |
| dc.subject.keyword | 石斑魚虹彩病毒,點帶石斑魚,啟動子,冷光酵素測定, | zh_TW |
| dc.subject.keyword | Epinephelus coioides,groupers,GIV,promoter,CD9,luciferase assay,poly(I:C),c-rel,NF-κB, | en |
| dc.relation.page | 49 | |
| dc.rights.note | 同意授權(全球公開) | |
| dc.date.accepted | 2013-08-19 | |
| dc.contributor.author-college | 生命科學院 | zh_TW |
| dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
| 顯示於系所單位： | 漁業科學研究所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-102-1.pdf | 20.5 MB | Adobe PDF | 檢視/開啟 |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。