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| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 張孟基(Men-Chi Chang) | |
| dc.contributor.author | Ching-Hua Chang | en |
| dc.contributor.author | 張靜華 | zh_TW |
| dc.date.accessioned | 2021-06-16T17:39:40Z | - |
| dc.date.available | 2017-08-28 | |
| dc.date.copyright | 2012-08-28 | |
| dc.date.issued | 2012 | |
| dc.date.submitted | 2012-08-14 | |
| dc.identifier.citation | 吳心平 (2008) 秈稉稻雜交BC1F1族群之不平衡分離。國立臺灣大學農藝學系碩士論文
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64303 | - |
| dc.description.abstract | 為提昇國產稻米之競爭力及區隔性並保持稻種純度，除了以外觀、生理或生化特性進行稻種鑑定外，以不同DNA分子標記產生之DNA指紋進行稻米品種鑑定已成為目前常用之分析技術。本研究以過去建立之國內外稻米品種單一核苷酸多型性 (Single nucleotide polymorphisms, SNPs)資料庫，篩選解析度最大之核心單一核苷酸多型性組(core SNP marker set)，發展多重單一核苷酸多型性之基因定型（multiplex SNP genotyping）分析平台以利國、內外稻米品種鑑定。本研究以多重PCR的方式，設計10或15個專一SNP位點之引子對於單一反應中進行多個PCR產物的增幅，再以毛細管電泳或電泳膠片分析產物大小，以確認該些SNP位點之多型性，並與己建立之不同稻米SNP資料庫互相比對進行稻種鑑識。本試驗嘗試以此技術應用於國內外水稻品種DNA資料庫之建立，市售稻米生產過程中容易產生品種混雜階段之檢出，並檢測不同生產階段的稻米混雜比率，作為稻米生產過程中合理混雜可能上限值之探討。台灣現有水稻品種DNA資料庫分析50個品種，結果發現其中21個品種具有一致的DNA指紋表現，19個品種有一主要表現型，而10個品種則具有2-3種主要表現型，顯示同一稻種內之DNA指紋表現均一度不佳，不易建立標準DNA指紋資料庫。另一方面，稻米生產過程中不可避免的混雜比率，若以分析結果最主要之DNA指紋為標準，顯示不論碾米廠規模大小、有無契作，異表現型的比率大致為10%左右。多重單一核苷酸多型性之基因定型技術雖有諸多限制性性，但若應用於鑑定稻米品種大量的刻意摻雜與混充，實為一有效的工具，亦可進一步推廣為國、內外米之檢測，促進稻米產業之健全發展。 | zh_TW |
| dc.description.abstract | In order to increase the competitiveness and marketing segment of Taiwan’s domestic rice, and maintain its genetic purity, the DNA fingerprinting technique which is based on DNA molecular markers for rice varieties identification has become a commonly used platform. In this study, we chose a core SNP marker set from previously established rice SNP database to develop a multiplex-SNP genotyping analysis platform for domestic and foreign rice varieties characterization. Using 10 or 15 sets of allelic-specific SNP primer pairs in the same PCR reaction and analysis PCR amplicons by capillary or regular gel electrophoresis, we were able to distinguish specific SNP loci among various rice cultivars. The DNA fingerprinting generated by multiplex-SNP genotyping analysis from rice grain sample can be further compared with available database to confirm specific rice identity. In this research, first, we attempted to establish an up-dated DNA fingerprinting database of domestic and foreign rice. From the genotyping analysis of 50 domestic rice varieties, we found that 21 varieties showed similar DNA fingerprinting pattern and 19 varieties showed one major type of DNA fingerprinting. However, 10 varieties had 2-3 major types of DNA fingerprinting profile, indicating that the results were too complicated to build up a thorough DNA database. Second, we tried to address the issue related to the assessment of admixture ratio along production processes and set up a standard for tolerance of mixed-rice ratio in rice market. The results showed that the acceptable admixture ratio can be as less as 10%. Multiplex SNP genotyping technique has some limitations, but it can provide a reliable and effective method for characterization and purity test of rice varieties. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T17:39:40Z (GMT). No. of bitstreams: 1 ntu-101-R99621113-1.pdf: 1919384 bytes, checksum: a113cce7e29cde2dac7800df40fdf4fb (MD5) Previous issue date: 2012 | en |
| dc.description.tableofcontents | 國立臺灣大學碩士學位論文口試委員會審定書..........................I
致謝......................................................II 中文摘要..................................................III 英文摘要...................................................IV 表目錄....................................................VII 圖目錄...................................................VIII 第一章、 前言...............................................1 第二章、 前人研究............................................4 一、水稻之品種鑑定與實際問題............................4 二、DNA分子標記與其優勢...............................4 三、分子標記的演進與應用...............................5 1. 第一代分子標記............................5 2. 第二代分子標記............................7 3. 第三代分子標記............................8 四、多重單一核苷酸多型性基因定型技術的應用...............10 五、本實驗室先前開發之稻種鑑定技術與相關資料庫............11 第三章、 材料與方法.........................................13 一、稻種材料........................................13 二、稻種取樣方式.....................................14 三、DNA萃取與定量...................................15 四、多重單一核苷酸多型性分子標記平台....................16 五、多重單一核苷酸多型性聚合酶連鎖反應...................17 六、電泳分析........................................18 1. 毛細管電泳..............................18 2. 膠體電泳................................19 第四章、 結果..............................................20 一、國內水稻品種多重單一核苷酸多型性之DNA指紋分析結果......20 二、台灣稻種DNA資料庫之建立...........................21 三、稻米生產過程中合理可容許混雜率上限值之探討............21 四、國外水稻品種多重單一核苷酸多型性之DNA指紋分析結果......22 第五章、 討論..............................................23 一、15-plex與10-plex稻種鑑定平台應用上之限制性.........23 二、台灣稻種姐妹系DNA指紋表現複雜，不易建立DNA資料庫......24 三、訂定稻米生產過程中可容許混雜率合理上限值可行性之探討....25 四、多重單一核苷酸多型性DNA指紋分析對國內稉米之鑑定能力較佳.26 五、展望...........................................27 第六章、 參考文獻...........................................28 | |
| dc.language.iso | zh-TW | |
| dc.title | 以多重單一核苷酸多型性基因定型技術建立臺灣稻米品種DNA指紋資料庫及其用於品種純度檢測之可行性研究 | zh_TW |
| dc.title | Study of the Applicability with the Multiplex SNP Genotyping Technology in Establishing DNA Fingerprinting Database and Monitoring the Purity of Taiwan Rice Varieties | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 100-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 林彥蓉,胡凱康,黃文理,羅正宗 | |
| dc.subject.keyword | 稻米品種鑑定,DNA指紋,多重單一核苷,酸多型性,姊妹系,混雜比率, | zh_TW |
| dc.subject.keyword | rice varieties identification,DNA fingerprinting,multiplex SNP,sister line,admixture ratio, | en |
| dc.relation.page | 64 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2012-08-15 | |
| dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
| dc.contributor.author-dept | 農藝學研究所 | zh_TW |
| Appears in Collections: | 農藝學系 | |
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| ntu-101-1.pdf Restricted Access | 1.87 MB | Adobe PDF |
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