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  1. NTU Theses and Dissertations Repository
  2. 工學院
  3. 醫學工程學研究所
請用此 Handle URI 來引用此文件: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64243
完整後設資料紀錄
DC 欄位值語言
dc.contributor.advisor趙本秀
dc.contributor.authorShou-Chien Shenen
dc.contributor.author沈守謙zh_TW
dc.date.accessioned2021-06-16T17:36:33Z-
dc.date.available2012-08-19
dc.date.copyright2012-08-19
dc.date.issued2012
dc.date.submitted2012-08-15
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dc.identifier.urihttp://tdr.lib.ntu.edu.tw/jspui/handle/123456789/64243-
dc.description.abstract細胞與其機械環境的交互作用在許多生理機制中扮演重要角色,包括形態發育,免疫反應, 傷口癒合以及腫瘤細胞惡性轉移等。近期研究顯示,細胞有優先朝機械性質較硬的區域去遷移的現象,稱為durotaxis。本研究的目的為發展一個有連續彈性梯度的系統,是以膠原蛋白膠體為基礎的複合微結構基材,來促使細胞產生durotaxis,並更進一步探討其機制。研究結果顯示細胞durotaxis的表現與其彈性梯度的強度相關。此外也發現durotaxis 只會出現在細胞密度較低時;細胞與細胞的交互作用會造成durotaxis表現下降。藥物抑制的研究顯示細胞受基材機械性質影響而產生方向性遷移的行為是與Rho及myosin所調控細胞收縮的機制相關。本系統為全新的實驗平台來幫助深入探討基材彈性梯度對細胞反應與其內部分子機制的影響。zh_TW
dc.description.abstractCell interaction with their physical environment has been shown to play an important role in physiological processes, including morphogenesis, immune response, wound healing, and tumor metastasis. Recent studies have demonstrated that preferential migration of cells toward mechanically stiff regions; a process known as durotaxis. However, much of this phenomenon is still not fully understood. In this study, we developed a novel system of a continuous stiffness gradient with a collagen hydrogel-based composite microstructural substrate. Our results indicated that induction of durotaxis depends on the magnitude of the elasticity jump. In addition, our results provide a quantitative confirmation that increases in cell density reduce cell durotaxis, which can be observed only at low cell density, where there is less cell-cell interaction. Pharmacologic inhibition studies suggested that cell migration directed by substrate mechanical stiffness was regulated by a Rho- and myosin-dependent cell contractility mechanism. This novel platform allows for better understanding of the cellular response to substrate stiffness gradients and the underlying molecular mechanisms.en
dc.description.provenanceMade available in DSpace on 2021-06-16T17:36:33Z (GMT). No. of bitstreams: 1
ntu-101-R99548045-1.pdf: 1868340 bytes, checksum: a1aed36c389a040fe96f621a5ce106f2 (MD5)
Previous issue date: 2012
en
dc.description.tableofcontents口試委員會審定書 2
致謝 3
摘要 i
Abstract ii
Chapter 1 Introduction 1
1.1. Cell migration 1
1.2. Durotaxis 3
1.3. Mechanotransduction 5
Experimental Design 6
Chapter 2 Materials and Methods 8
2.1 Cell Culture 8
2.2 Composite substrate preparation 8
2.3 Collagen hydrogel preparation 9
2.4 Measurement of the surface elasticity distribution 9
2.5 Time-lapse observation of Cell Behavior 10
2.6 Immunofluorescence microscopic observation 10
2.7 Pharmacological inhibition 11
2.8 Statistical Analysis 12
Chapter 3 Results 13
Chapter 4 Discussion 18
Chapter 5 Conclusions 23
Reference 41
Appendix 46
dc.language.isoen
dc.title以複合式微結構平台來探討細胞在彈性梯度上的移動行為zh_TW
dc.titleA Composite Microstructural Substrate with Stiffness Gradient for the Study of Cell Migrationen
dc.typeThesis
dc.date.schoolyear100-2
dc.description.degree碩士
dc.contributor.oralexamcommittee王兆麟,蔡偉博,郭柏齡
dc.subject.keyword細胞遷移,durotaxis,彈性梯度,mechanosensing,細胞骨架,細胞力學,原子力顯微鏡,zh_TW
dc.subject.keywordCell migration,durotaxis,stiffness gradient,mechanosensing,cytoskeleton,cell mechanics,atomic force microscopy,en
dc.relation.page49
dc.rights.note有償授權
dc.date.accepted2012-08-15
dc.contributor.author-college工學院zh_TW
dc.contributor.author-dept醫學工程學研究所zh_TW
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