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標題: | 整合無稀釋血漿分離微流道及電化學阻抗頻譜分析應用於進行原位生物標記物檢測 A Microfluidics Device for Dilution-free Plasma Extraction and In-situ Biomarkers Detection Using Electrochemical Impedance Spectroscopy Sensor |
作者: | Chia-Chien Wu 吳佳蒨 |
指導教授: | 黃念祖(Nien-Tsu Huang) |
關鍵字: | 微流道,全血處理,血漿萃取,電化學阻抗頻譜分析, Microfluidics,Whole blood processing,Plasma extraction,Electrochemical Impedance Spectroscopy, |
出版年 : | 2020 |
學位: | 碩士 |
摘要: | 血液檢測為現今最常用來作為疾病診斷及評斷身體健康狀況的方式之一。但傳統的血液檢查通常需要到醫院進行抽血,且血液的分析需要花費數個小時。分析的過程也需要由專業人員以大型量測儀器進行繁複的操作。因此,我們希望研發一個整合電化學阻抗頻譜分析(Electrochemical Impedance Spectroscopy, EIS)的微流道裝置針對微升體積的血液做血漿萃取,並針對血漿中的蛋白質進行濃度分析。本裝置主要分成兩個部分,血漿萃取及蛋白質量測。在血漿萃取的部分,為了加快沉降速度並提高產率,我們在微流道當中加入了紅血球凝集因子,並在流道中加入了直徑0.2 微米的濾膜提取更高純度的血漿。對於多數成年人血液樣本而言,我們的裝置可以在7分鐘內,從50微升不做任何前處理的的全血中萃取出30%的血漿。除此之外,萃取的血漿的細胞移除率可以達到近乎100%,且透過量測血紅素的光譜,可以證明在萃取的過程中不存在血球裂解的問題。接著而蛋白質量測部分,我們透過微流道將萃取出來的血漿引導至電化學感測器的感測範圍內進行反應。整個反應的過程可以在30分鐘完成。整個操作的過程不需要專業人員的操作,也沒有樣本轉移的問題。因此我們相信此快速且可攜式儀器將會有助於未來個人化醫療的發展。 Blood analysis is one of the most common tests for disease diagnosis or physiological conditions monitoring. However, traditional blood analysis methods often require professional technicians to extract whole blood from patients with bulky instruments and tedious processes. To address these problems, we propose to integrate Electrochemical Impedance Spectroscopy (EIS) sensor with a microfluidics system for whole blood processing. The system is separated into two parts, plasma extraction, and protein sensing. For plasma extraction, we inject red blood cell (RBC) agglutination reagent to the device to accelerate the erythrocyte sedimentation rate (ESR). Also, a 0.2 μm membrane is embedded in the microfluidic channel to enhance the purity of the extracted plasma. For most of the clinical samples, our device can extract 30% of the plasma from 50 μL whole blood without any sample pretreatment in 7 minutes. The cell removal rate is almost 100% and no hemolysis effect is generated during the process. As for protein sensing, the extracted plasma is guided to the sensing area of the EIS sensor. The incubation time is about 30 minutes and the result can be shown without the aim of the professional medical examiner. Therefore, we believe that due to the rapidness in the detection and the portability of the system, our device has the potential to develop into a point-of-care (POC) system. |
URI: | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63927 |
DOI: | 10.6342/NTU202000692 |
全文授權: | 有償授權 |
顯示於系所單位: | 生醫電子與資訊學研究所 |
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