Skip navigation

DSpace JSPUI

DSpace preserves and enables easy and open access to all types of digital content including text, images, moving images, mpegs and data sets

Learn More
DSpace logo
English
中文
  • Browse
    • Communities
      & Collections
    • Publication Year
    • Author
    • Title
    • Subject
  • Search TDR
  • Rights Q&A
    • My Page
    • Receive email
      updates
    • Edit Profile
  1. NTU Theses and Dissertations Repository
  2. 生命科學院
  3. 生化科技學系
Please use this identifier to cite or link to this item: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63853
Title: Ankaflavin 對高脂飲食下動物胰島素訊號傳遞及脂質累積之影響
The effects of ankaflavin treatment on insulin signaling and lipid accumulation in high-fat diet mice
Authors: Te-Han Liao
廖德翰
Advisor: 潘子明(Tzu-Ming Pan)
Keyword: 3T3-L1 前脂肪細胞株,高脂飲食,胰島素阻抗,adipocytokines,
3T3-L1 preadipocyte,high-fat diet,insulin resistance,adipocytokines,
Publication Year : 2012
Degree: 碩士
Abstract: 本研究以 ankaflavin 配合高脂飲食誘導模式,並同時以腹腔注射 GW9662 (PPAR gamma 拮抗劑),評估 ankaflavin 對於動物脂肪堆積與胰島素敏感度的調控。結果發現,ankaflavin 能藉由調節PPAR gamma 以降低高脂飲食誘導產生之高血糖及高胰島素症,其降低率分別為 36.6% 及 60%,並可減少血清與肝組織中三酸甘油酯與總膽固醇。在細胞實驗中以 ankaflavin 與另一結構相似之黃色素monascin 處理 3T3-L1 小鼠成熟脂肪細胞,結果顯示 ankaflavin 與 monascin可活化脂肪細胞胰島素訊號傳遞並促進葡萄糖吞噬 (glucose uptake)。此外,本研究首次證實ankaflavin 可藉由PPAR gamma 調節脂肪細胞大小與數目,並減少脂肪細胞平均半徑,提升脂肪細胞生產 adiponectin 並降低 resistin 及 leptin。顯示 ankaflavin 可能藉由調控脂肪組織對胰島素敏感度,並活化 adipokines 下游路徑而達到改善高脂誘導胰島素抗性。
In order to investigate whether ankaflavin can regulate lipid metabolism and insulin sensitivity through PPAR gamma pathways, we fed mice with high-fat diet and ankaflavin and performed i.p. injection of GW9662 (PPAR gamma antagonist) simultaneously. In vitro, we examined the expressions of insulin signaling proteins in differentiated 3T3-L1 cells treated with ankaflavin and another structural similar Monascus yellow pigment—monascin. Results showed that ankaflavin down-regulated triglyceride and total cholesterol in serum and liver tissue. Ankaflavin could also ameliorate hyperglycemia and hyperinsulinemia symptoms elicited by high-fat diet, the decrease ratio was 36.6% and 60% respectively, which might be due to its abilities to improve insulin signaling and glucose uptake in differentiated 3T3-L1 cells. Moreover, we discovered for the first time that ankaflavin could regulate the size and the amount of adipocytes in a PPAR gamma dependent way, and reduce average radius of adipocytes as well. Also, ankaflavin elevate the levels of adiponectin and decrease levels of resistin and leptin via PPAR gamma pathways in vivo and in vitro, which might activate signalings related to lipid metabolism and insulin signaling.
URI: http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63853
Fulltext Rights: 有償授權
Appears in Collections:生化科技學系

Files in This Item:
File SizeFormat 
ntu-101-1.pdf
  Restricted Access
5.56 MBAdobe PDF
Show full item record


Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.

社群連結
聯絡資訊
10617臺北市大安區羅斯福路四段1號
No.1 Sec.4, Roosevelt Rd., Taipei, Taiwan, R.O.C. 106
Tel: (02)33662353
Email: ntuetds@ntu.edu.tw
意見箱
相關連結
館藏目錄
國內圖書館整合查詢 MetaCat
臺大學術典藏 NTU Scholars
臺大圖書館數位典藏館
本站聲明
© NTU Library All Rights Reserved