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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 洪傳揚(Chwan-Yang Hong) | |
dc.contributor.author | Yu-hsiang Lin | en |
dc.contributor.author | 林煜翔 | zh_TW |
dc.date.accessioned | 2021-05-11T04:50:24Z | - |
dc.date.available | 2019-08-20 | |
dc.date.available | 2021-05-11T04:50:24Z | - |
dc.date.copyright | 2019-08-20 | |
dc.date.issued | 2019 | |
dc.date.submitted | 2019-08-15 | |
dc.identifier.citation | Cancer, I. A. f. R. o. (1993). Beryllium, cadmium, mercury, and exposures in the glass. Apresentado em: IARC Working Group on the Evaluation of Carcinogenic Risks to Humans: Beryllium, Lyon, 1993.
Chmielowska-Bąk, J., Gzyl, J., Rucińska-Sobkowiak, R., Arasimowicz-Jelonek, M., and Deckert, J. (2014). The new insights into cadmium sensing. Frontiers in plant science 5, 245. Colangelo, E. P., and Guerinot, M. L. (2006). Put the metal to the petal: metal uptake and transport throughout plants. Current opinion in plant biology 9, 322-330. CURIE, C., ALONSO, J. M., Marie, L., ECKER, J. R., and BRIAT, J.-F. (2000). Involvement of NRAMP1 from Arabidopsis thaliana in iron transport. Biochemical Journal 347, 749-755. Foyer, C. H., and Noctor, G. (2005). Redox homeostasis and antioxidant signaling: a metabolic interface between stress perception and physiological responses. The Plant Cell 17, 1866-1875. Hernandez, L., Carpena‐Ruiz, R., and Garate, A. (1996). Alterations in the mineral nutrition of pea seedlings exposed to cadmium. Journal of Plant Nutrition 19, 1581-1598. Iimura, K. (1978). Behavior and balance of contaminant heavy metals in paddy soils-studies on heavy metal pollution of soils (part 2). Bull Hokuriku Natl Agric Exp Stn 21, 95-145. Inaba, T., Kobayashi, E., Suwazono, Y., Uetani, M., Oishi, M., Nakagawa, H., and Nogawa, K. (2005). Estimation of cumulative cadmium intake causing Itai-itai disease. Toxicol Lett 159, 192-201. Iqbal, M., Mahmood, M. T., and Ahmed, F. (1991). Influence of cadmium toxicity on germination and growth of some common trees. Pakistan Journal of Scientific and Industrial Research (Pakistan). Ishikawa, S., Abe, T., Kuramata, M., and Hayashi, S. (2019). Development of Low-Cadmium-Accumulating Rice. In 'Cadmium Toxicity', pp. 139-150. Springer. Ishikawa, S., Ishimaru, Y., Igura, M., Kuramata, M., Abe, T., Senoura, T., Hase, Y., Arao, T., Nishizawa, N. K., and Nakanishi, H. (2012). Ion-beam irradiation, gene identification, and marker-assisted breeding in the development of low-cadmium rice. Proceedings of the National Academy of Sciences 109, 19166-19171. Ishikawa, S., Makino, T., Ito, M., Harada, K., Nakada, H., Nishida, I., Nishimura, M., Tokunaga, T., Shirao, K., and Yoshizawa, C. (2016). Low-cadmium rice (Oryza sativa L.) cultivar can simultaneously reduce arsenic and cadmium concentrations in rice grains. Soil science and plant nutrition 62, 327-339. Ishimaru, Y., Takahashi, R., Bashir, K., Shimo, H., Senoura, T., Sugimoto, K., Ono, K., Yano, M., Ishikawa, S., and Arao, T. (2012). Characterizing the role of rice NRAMP5 in manganese, iron and cadmium transport. Scientific reports 2, 286. Ito, H., and Iimura, K. (1976). The absorption and translocation of cadmium in rice plants and its influence on their growth, in comparison with zinc: Studies on heavy metal pollution of soils (Part 1). Bull Hokuriku Natl Agric Exp Stn 19, 71-139. Klaassen, C. D. (1981). Pharmacokinetics in metal toxicity. Fundamental and Applied Toxicology 1, 353-357. Klaassen, C. D., Liu, J., and Choudhuri, S. (1999). Metallothionein: an intracellular protein to protect against cadmium toxicity. Annual review of pharmacology and toxicology 39, 267-294. Kobayashi, A., Hori, K., Yamamoto, T., and Yano, M. (2018). Koshihikari: a premium short-grain rice cultivar–its expansion and breeding in Japan. Rice 11, 15. Lanquar, V., Lelièvre, F., Bolte, S., Hamès, C., Alcon, C., Neumann, D., Vansuyt, G., Curie, C., Schröder, A., and Krämer, U. (2005). Mobilization of vacuolar iron by AtNRAMP3 and AtNRAMP4 is essential for seed germination on low iron. The EMBO journal 24, 4041-4051. Møller, I. M. (2001). Plant mitochondria and oxidative stress: electron transport, NADPH turnover, and metabolism of reactive oxygen species. Annual review of plant biology 52, 561-591. Mammadov, J., Aggarwal, R., Buyyarapu, R., and Kumpatla, S. (2012). SNP markers and their impact on plant breeding. International journal of plant genomics 2012. Nakanishi, H., Ogawa, I., Ishimaru, Y., Mori, S., and Nishizawa, N. K. (2006). Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice. Soil Science & Plant Nutrition 52, 464-469. Nevo, Y., and Nelson, N. (2006). The NRAMP family of metal-ion transporters. Biochimica et Biophysica Acta (BBA)-Molecular Cell Research 1763, 609-620. Pandey, N., and Sharma, C. P. (2002). Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Plant Science 163, 753-758. Pedas, P., Ytting, C. K., Fuglsang, A. T., Jahn, T. P., Schjoerring, J. K., and Husted, S. (2008). Manganese efficiency in barley: identification and characterization of the metal ion transporter HvIRT1. Plant physiology 148, 455-466. Perfus‐Barbeoch, L., Leonhardt, N., Vavasseur, A., and Forestier, C. (2002). Heavy metal toxicity: cadmium permeates through calcium channels and disturbs the plant water status. The Plant Journal 32, 539-548. Poschenrieder, C., Gunse, B., and Barcelo, J. (1989). Influence of cadmium on water relations, stomatal resistance, and abscisic acid content in expanding bean leaves. Plant Physiology 90, 1365-1371. Sasaki, A., Yamaji, N., Yokosho, K., and Ma, J. F. (2012). Nramp5 is a major transporter responsible for manganese and cadmium uptake in rice. The Plant Cell 24, 2155-2167. Takao, W., Shimbob, S., Moon, C., S., Zhang, Z., W., and Ikeda, M. (1996). Cadmium contents in rice samples from various areas in the world. The Science of the Total Environment 184, 191-196. Tang, L., Mao, B., Li, Y., Lv, Q., Zhang, L., Chen, C., He, H., Wang, W., Zeng, X., and Shao, Y. (2017). Knockout of OsNramp5 using the CRISPR/Cas9 system produces low Cd-accumulating indica rice without compromising yield. Scientific reports 7, 14438. Thomine, S., Lelièvre, F., Debarbieux, E., Schroeder, J. I., and Barbier‐Brygoo, H. (2003). AtNRAMP3, a multispecific vacuolar metal transporter involved in plant responses to iron deficiency. The Plant Journal 34, 685-695. Thomine, S., Wang, R., Ward, J. M., Crawford, N. M., and Schroeder, J. I. (2000). Cadmium and iron transport by members of a plant metal transporter family in Arabidopsis with homology to Nramp genes. Proceedings of the National Academy of Sciences 97, 4991-4996. Vidal, S. M., Malo, D., Vogan, K., Skamene, E., and Gros, P. (1993). Natural resistance to infection with intracellular parasites: isolation of a candidate for Bcg. Cell 73, 469-485. Waalkes, M., and Berthan, G. (1995). Handbook on metal-ligand interactions of biological fluids. Marcel Dekker, New York. Waalkes, M., Misra, R., and Chang, L. (1996). Toxicology of metals. Boca Raton, FL: CRC. Xia, J., Yamaji, N., Kasai, T., and Ma, J. F. (2010). Plasma membrane-localized transporter for aluminum in rice. Proceedings of the National Academy of Sciences 107, 18381-18385. YANG, C.-h., ZHANG, Y., and HUANG, C.-f. (2019). Reduction in cadmium accumulation in japonica rice grains by CRISPR/Cas9-mediated editing of OsNRAMP5. Journal of integrative agriculture 18, 688-697 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/handle/123456789/635 | - |
dc.description.abstract | 鎘 (Cd) 非植物的必須元素,卻容易經由必須元素通道累積在水稻穀粒中,鎘米會造成人體肝、腎的慢性毒害,因此控制穀粒的鎘含量對於糧食安全極為重要。在水稻吸收鎘的機制中,Natural resistance-associated macrophage protein 5 (OsNramp5) 運輸蛋白是水稻根部吸收鎘最主要的通道,過去的研究已知, OsNramp5 基因被離子束誘變法剔除時,可大幅降低水稻穀粒鎘含量,而利用基因編輯技術剔除OsNramp5 的水稻植株也同樣可獲得含鎘量極低的穀粒,這些結果顯示OsNramp5 是影響水稻穀粒鎘累積最關鍵的基因。本研究目的在使台灣本土水稻品種也具有低鎘累積特性,研究從兩個方向進行:(1)利用單核苷酸多型性資料庫篩選 OsNramp5 核苷酸具有變異的水稻品系,以作為育成低鎘水稻的種源;(2)應用基因編輯技術剔除台灣水稻品種的 OsNramp5 基因。從水稻單核苷酸多態性資料庫中,篩選出 50 個 OsNramp5 核苷酸具有變異的水稻品系,分析這些品系OsNramp5 胺基酸序列,發現有十個胺基酸序列改變的品系,具有穀粒低鎘累積潛力,其中 NSF-20、NSF-21 及 Nona Bokra 三個水稻都出現第 505 個胺基酸由丙胺酸 (Alanine, A, 基因編碼為 GCC) 變為蘇胺酸 (Threonine, T, 基因編碼為 ACC)之突變,分析鎘處理後 50 個品系幼苗地上部、根部的鎘累積量,發現 8 種鎘含量較低的品系。基因編輯試驗中,選擇台灣目前栽培面積最廣的台南 11 號、及研究資源最多的台農67 號進行基因編輯,針對 OsNramp5 基因第十個外顯子進行編輯,再利用基因定序篩選編輯成功的植株,未來可將進一步種植這些具有低鎘累積潛力的水稻於含鎘土壤,以評估具有低鎘累積潛力之水稻品系。 | zh_TW |
dc.description.abstract | Cd is a toxic heavy metal and can lead to Cd-related diseases such as renal tubular dysfunction and bone disease. Cadmium (Cd) is not an essential element for plants. However, rice uptakes and accumulates Cd in grains by essential element’s transporters. The accumulation of Cd is a serious threat to human being since it can be concentrated in body through the food chain. Therefore, reducing the Cd content in grains is very important for food safety. Natural resistance-associated macrophage protein 5 (OsNramp5) is the main transporter which uptake Cd in roots. Knocking out of OsNramp5 has been reported to dramatically reduced Cd accumulation in rice grains. To generate low Cd accumulating Taiwanese cultivars, this research ainmed to carry out in two ways: 1. Screening for osnramp5 dysfunction mutants from single nucleotide polymorphism (SNP) database. 2. Genome-editing the OsNramp5 to Taiwanese cultivars. After screening SNP database from the rice 3000 genomes, 50accessions showed polymorphic genotypes on OsNramp5 genes. Analysis of amino acid sequences from 50 selected accessions showed that 10 accessions contained a change of amino acid. . In addition, three of ten accessions showed the same change at 505th amino acid, where Alanine (Ala, GCC) was substituted by Threonine (Thr, ACC). Examined of Cd concentrations on roots and shoots of rice seedlings showed that 8 lines have lower Cd content in roots or shoots. To generate genome-edited rice plants, two domestic rice cultivars, Tainan 11 (TN11) and Tainung 67 (TNG67), were used to precisely mutagenized the OsNramp5. Tainan 11 (TN11) is the most growing rice cultivars, while Tainung 67 (TNG67) has the most available research resources. Both cultivars’ 9th exon of OsNramp5 was chose for CRISPR/Cas9–based mutagenesis. Mutation was determined by DNA sequencing. Identification of OsNramp5 mutated accessions can be used as a parent for low Cadmium rice breeding. On the other hand, genome-edited rice plants can further screen low cadmium and transgene-free progenies. | en |
dc.description.provenance | Made available in DSpace on 2021-05-11T04:50:24Z (GMT). No. of bitstreams: 1 ntu-108-R06623014-1.pdf: 1406675 bytes, checksum: 0ca005a20a45853663bff72a8f1823b8 (MD5) Previous issue date: 2019 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iii 目錄 v 圖目錄 vii 表目錄 viii 縮寫對照表 ix 第壹章、緒論 1 一、鎘對人造成的危害 1 二、鎘對植物的影響 2 三、鎘與水稻的關係 3 四、現行鎘污染場址整治 3 五、藉改變栽培方法降低水稻穀粒鎘的累積 4 六、低鎘水稻的育成 5 七、水稻的核苷酸多態性 6 八、基因編輯技術 7 第貳章、研究目的 10 第參章、材料與方法 11 一、植物材料與生長條件 11 二、DNA 定序 11 三、基因表現分析材料準備與處理 13 四、基因表現分析 14 五、水耕試驗及幼苗鎘含量分析 15 第肆章、結果 17 一、OsNramp5的基因結構與胺基酸突變位點 17 二、50 株栽培種的相關資訊 17 三、50 株栽培種的基因定序及胺基酸變化 17 四、50 株栽培種的OsNramp5 表現量 18 五、鎘處理下水稻幼苗的植體鎘含量 18 六、CRISPR 轉植株的轉基因檢測 18 七、CRISPR 轉植株的基因編輯成果 18 第伍章、討論 19 第陸章、參考文獻 21 第柒章、附錄 24 | |
dc.language.iso | zh-TW | |
dc.title | 利用單核苷酸多型性資料庫及基因編輯技術建立 OsNramp5 基因突變水稻 | zh_TW |
dc.title | Generation of OsNramp5 Dysfunction Rice Mutants Using Single Nucleotide Polymorphism Database and Genome Editing Technology | en |
dc.date.schoolyear | 107-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 陸重安,黃文理,林雅芬,張孟基 | |
dc.subject.keyword | 鎘,水稻,OsNramp5 基因,單核?酸多態性,CRISPR 基因編輯, | zh_TW |
dc.subject.keyword | Cadmium,Rice,Nramp5,SNP,CRISPR, | en |
dc.relation.page | 36 | |
dc.identifier.doi | 10.6342/NTU201903620 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2019-08-16 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農業化學研究所 | zh_TW |
顯示於系所單位: | 農業化學系 |
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