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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 阮雪芬 | |
dc.contributor.author | Shiao-Yun Ko | en |
dc.contributor.author | 柯曉雲 | zh_TW |
dc.date.accessioned | 2021-06-16T17:13:46Z | - |
dc.date.available | 2017-08-22 | |
dc.date.copyright | 2012-08-22 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2012-08-20 | |
dc.identifier.citation | 1 R. Aebersold, and M. Mann, 'Mass Spectrometry-Based Proteomics', Nature, 422 (2003), 198-207.
2 M. V. Alinsug, F. F. Chen, M. Luo, R. Tai, L. W. Jiang, and K. Q. Wu, 'Subcellular Localization of Class Ii Hdas in Arabidopsis Thaliana: Nucleocytoplasmic Shuttling of Hda15 Is Driven by Light', Plos One, 7 (2012). 3 K. Baerenfaller, J. Grossmann, M. A. Grobei, R. Hull, M. Hirsch-Hoffmann, S. Yalovsky, P. Zimmermann, U. Grossniklaus, W. Gruissem, and S. Baginsky, 'Genome-Scale Proteomics Reveals Arabidopsis Thaliana Gene Models and Proteome Dynamics', Science, 320 (2008), 938-41. 4 F. Bao, J. J. Shen, S. R. Brady, G. K. Muday, T. Asami, and Z. B. Yang, 'Brassinosteroids Interact with Auxin to Promote Lateral Root Development in Arabidopsis', Plant Physiol, 134 (2004), 1624-31. 5 P. Benveniste, 'Sterol Biosynthesis', Annual Review of Plant Physiology and Plant Molecular Biology, 37 (1986), 275-308. 6 P. J. Boersema, R. Raijmakers, S. Lemeer, S. Mohammed, and A. J. R. Heck, 'Multiplex Peptide Stable Isotope Dimethyl Labeling for Quantitative Proteomics', Nat Protoc, 4 (2009), 484-94. 7 Y. Chen, W. Hoehenwarter, and W. Weckwerth, 'Comparative Analysis of Phytohormone-Responsive Phosphoproteins in Arabidopsis Thaliana Using Tio2-Phosphopeptide Enrichment and Mass Accuracy Precursor Alignment', Plant Journal, 63 (2010), 1-17. 8 S. D. Clouse, 'Brassinosteroid Signal Transduction: Clarifying the Pathway from Ligand Perception to Gene Expression', Molecular Cell, 10 (2002), 973-82. 9 S. D. Clouse, M. Langford, and T. C. McMorris, 'A Brassinosteroid-Insensitive Mutant in Arabidopsis Thaliana Exhibits Multiple Defects in Growth and Development', Plant Physiol, 111 (1996), 671-8. 10 S. D. Clouse, and J. M. Sasse, 'Brassinosteroids: Essential Regulators of Plant Growth and Development', Annual Review of Plant Physiology and Plant Molecular Biology, 49 (1998), 427-51. 11 S. Di Rubbo, N. G. Irani, and E. Russinova, 'Pp2a Phosphatases: The 'on-Off' Regulatory Switches of Brassinosteroid Signaling', Science Signaling, 4 (2011). 12 S. S. Gampala, T. W. Kim, J. X. He, W. Q. Tang, Z. P. Deng, M. Y. Bai, S. H. Guan, S. Lalonde, Y. Sun, J. M. Gendron, H. J. Chen, N. Shibagaki, R. J. Ferl, D. Ehrhardt, K. Chong, A. L. Burlingame, and Z. Y. Wang, 'An Essential Role for 14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis', Developmental Cell, 13 (2007), 177-89. 13 H. Goda, S. Sawa, T. Asami, S. Fujioka, Y. Shimada, and S. Yoshida, 'Comprehensive Comparison Brassinosteroid-Regulated of Auxin-Regulated and Brassinosteroid-Regulated Genes in Arabidopsis', Plant Physiol, 134 (2004), 1555-73. 14 H. Goda, Y. Shimada, T. Asami, S. Fujioka, and S. Yoshida, 'Microarray Analysis of Brassinosteroid-Regulated Genes in Arabidopsis', Plant Physiol, 130 (2002), 1319-34. 15 M. D. Grove, G. F. Spencer, W. K. Rohwedder, N. Mandava, J. F. Worley, J. D. Warthen, G. L. Steffens, J. L. Flippenanderson, and J. C. Cook, 'Brassinolide, a Plant Growth-Promoting Steroid Isolated from Brassica-Napus Pollen', Nature, 281 (1979), 216-17. 16 C. Guillet, M. A. Aboul-Soud, A. Le Menn, N. Viron, A. Pribat, V. Germain, D. Just, P. Baldet, P. Rousselle, M. Lemaire-Chamley, and C. Rothan, 'Regulation of the Fruit-Specific Pep Carboxylase Slppc2 Promoter at Early Stages of Tomato Fruit Development', Plos One, 7 (2012), e36795. 17 J. E. Harthill, S. E. M. Meek, N. Morrice, M. W. Peggie, J. Borch, B. H. C. Wong, and C. MacKintosh, 'Phosphorylation and 14-3-3 Binding of Arabidopsis Trehalose-Phosphate Synthase 5 in Response to 2-Deoxyglucose', Plant Journal, 47 (2006), 211-23. 18 J. F. Hess, R. B. Bourret, and M. I. Simon, 'Histidine Phosphorylation and Phosphoryl Group Transfer in Bacterial Chemotaxis', Nature, 336 (1988), 139-43. 19 J. A. Hoch, 'Two-Component and Phosphorelay Signal Transduction', Current Opinion in Microbiology, 3 (2000), 165-70. 20 B. Huang, C. H. Chu, S. L. Chen, H. F. Juan, and Y. M. Chen, 'A Proteomics Study of the Mung Bean Epicotyl Regulated by Brassinosteroids under Conditions of Chilling Stress', Cellular & Molecular Biology Letters, 11 (2006), 264-78. 21 Y. Ishihama, J. Rappsilber, and M. Mann, 'Modular Stop and Go Extraction Tips with Stacked Disks for Parallel and Multidimensional Peptide Fractionation in Proteomics', Journal of Proteome Research, 5 (2006), 988-94. 22 H. Jin, Z. Hong, W. Su, and J. M. Li, 'A Plant-Specific Calreticulin Is a Key Retention Factor for a Defective Brassinosteroid Receptor in the Endoplasmic Reticulum', Proceedings of the National Academy of Sciences of the United States of America, 106 (2009), 13612-17. 23 J. F. Johnson, C. P. Vance, and D. L. Allan, 'Phosphorus Deficiency in Lupinus Albus - Altered Lateral Root Development and Enhanced Expression of Phosphoenolpyruvate Carboxylase', Plant Physiol, 112 (1996), 31-41. 24 S. Kaul, H. L. Koo, J. Jenkins, M. Rizzo, T. Rooney, L. J. Tallon, T. Feldblyum, W. Nierman, M. I. Benito, X. Y. Lin, C. D. Town, J. C. Venter, C. M. Fraser, S. Tabata, Y. Nakamura, T. Kaneko, S. Sato, E. Asamizu, T. Kato, H. Kotani, S. Sasamoto, J. R. Ecker, A. Theologis, N. A. Federspiel, C. J. Palm, B. I. Osborne, P. Shinn, A. B. Conway, V. S. Vysotskaia, K. Dewar, L. Conn, C. A. Lenz, C. J. Kim, N. F. Hansen, S. X. Liu, E. Buehler, H. Altafi, H. Sakano, P. Dunn, B. Lam, P. K. Pham, Q. Chao, M. Nguyen, G. X. Yu, H. M. Chen, A. Southwick, J. M. Lee, M. Miranda, M. J. Toriumi, R. W. Davis, R. Wambutt, G. Murphy, A. Dusterhoft, W. Stiekema, T. Pohl, K. D. Entian, N. Terryn, G. Volckaert, M. Salanoubat, N. Choisne, M. Rieger, W. Ansorge, M. Unseld, B. Fartmann, G. Valle, F. Artiguenave, J. Weissenbach, F. Quetier, R. K. Wilson, M. de la Bastide, M. Sekhon, E. Huang, L. Spiegel, L. Gnoj, K. Pepin, J. Murray, D. Johnson, K. Habermann, N. Dedhia, L. Parnell, R. Preston, L. Hillier, E. Chen, M. Marra, R. Martienssen, W. R. McCombie, K. Mayer, O. White, M. Bevan, K. Lemcke, T. H. Creasy, C. Bielke, B. Haas, D. Haase, R. Maiti, S. Rudd, J. Peterson, H. Schoof, D. Frishman, B. Morgenstern, P. Zaccaria, M. Ermolaeva, M. Pertea, J. Quackenbush, N. Volfovsky, D. Y. Wu, T. M. Lowe, S. L. Salzberg, H. W. Mewes, S. Rounsley, D. Bush, S. Subramaniam, I. Levin, S. Norris, R. Schmidt, A. Acarkan, I. Bancroft, F. Quetier, A. Brennicke, J. A. Eisen, T. Bureau, B. A. Legault, Q. H. Le, N. Agrawal, Z. Yu, R. Martienssen, G. P. Copenhaver, S. Luo, C. S. Pikaard, D. Preuss, I. T. Paulsen, M. Sussman, A. B. Britt, D. A. Selinger, R. Pandey, D. W. Mount, V. L. Chandler, R. A. Jorgensen, C. Pikaard, G. Juergens, E. M. Meyerowitz, A. Theologis, J. Dangl, J. D. G. Jones, M. Chen, J. Chory, M. C. Somerville, and Ar Gen In, 'Analysis of the Genome Sequence of the Flowering Plant Arabidopsis Thaliana', Nature, 408 (2000), 796-815. 25 T. W. Kim, S. H. Guan, Y. Sun, Z. P. Deng, W. Q. Tang, J. X. Shang, Y. Sun, A. L. Burlingame, and Z. Y. Wang, 'Brassinosteroid Signal Transduction from Cell-Surface Receptor Kinases to Nuclear Transcription Factors', Nature Cell Biology, 11 (2009), 1254-U233. 26 T. Kinoshita, A. C. Cano-Delgado, H. Seto, S. Hiranuma, S. Fujioka, S. Yoshida, and J. Chory, 'Binding of Brassinosteroids to the Extracellular Domain of Plant Receptor Kinase Bri1', Nature, 433 (2005), 167-71. 27 Y. Kyono, N. Sugiyama, M. Tomita, and Y. Ishihama, 'Chemical Dephosphorylation for Identification of Multiply Phosphorylated Peptides and Phosphorylation Site Determination', Rapid Commun Mass Spectrom, 24 (2010), 2277-82. 28 H. M. O. Leyser, F. B. Pickett, S. Dharmasiri, and M. Estelle, 'Mutations in the Axr3 Gene of Arabidopsis Result in Altered Auxin Response Including Ectopic Expression from the Saur-Ac1 Promoter', Plant Journal, 10 (1996), 403-13. 29 J. M. Li, P. Nagpal, V. Vitart, T. C. McMorris, and J. Chory, 'A Role for Brassinosteroids in Light-Dependent Development of Arabidopsis', Science, 272 (1996), 398-401. 30 M. Menges, and J. A. H. Murray, 'Synchronous Arabidopsis Suspension Cultures for Analysis of Cell-Cycle Gene Activity', Plant Journal, 30 (2002), 203-12. 31 J. W. Mitchell, N. Mandava, J. F. Worley, J. R. Plimmer, and M. V. Smith, 'Brassins - a New Family of Plant Hormones from Rape Pollen', Nature, 225 (1970), 1065-&. 32 K. Mockaitis, and S. H. Howell, 'Auxin Induces Mitogenic Activated Protein Kinase (Mapk) Activation in Roots of Arabidopsis Seedlings', Plant Journal, 24 (2000), 785-96. 33 S. Mora-Garcia, G. Vert, Y. Yin, A. Cano-Delgado, H. Cheong, and J. Chory, 'Nuclear Protein Phosphatases with Kelch-Repeat Domains Modulate the Response to Brassinosteroids in Arabidopsis', Genes Dev, 18 (2004), 448-60. 34 C. F. Mouchel, G. C. Briggs, and C. S. Hardtke, 'Natural Genetic Variation in Arabidopsis Identifies Brevis Radix, a Novel Regulator of Cell Proliferation and Elongation in the Root', Genes & Development, 18 (2004), 700-14. 35 C. F. Mouchel, K. S. Osmont, and C. S. Hardtke, 'Brx Mediates Feedback between Brassinosteroid Levels and Auxin Signalling in Root Growth', Nature, 443 (2006), 458-61. 36 C. Mussig, G. H. Shin, and T. Altmann, 'Brassinosteroids Promote Root Growth in Arabidopsis', Plant Physiol, 133 (2003), 1261-71. 37 H. Nakagami, N. Sugiyama, K. Mochida, A. Daudi, Y. Yoshida, T. Toyoda, M. Tomita, Y. Ishihama, and K. Shirasu, 'Large-Scale Comparative Phosphoproteomics Identifies Conserved Phosphorylation Sites in Plants', Plant Physiol, 153 (2010), 1161-74. 38 A. Nakamura, Y. Shimada, H. Goda, M. T. Fujiwara, T. Asami, and S. Yoshida, 'Axr1 Is Involved in Br-Mediated Elongation and Saur-Ac1 Gene Expression in Arabidopsis', Febs Letters, 553 (2003), 28-32. 39 Y. Ogata, N. Sakurai, K. Aoki, H. Suzuki, K. Okazaki, K. Saito, and D. Shibata, 'Kagiana: An Excel-Based Tool for Retrieving Summary Information on Arabidopsis Genes', Plant and Cell Physiology, 50 (2009), 173-77. 40 S. E. Ong, and M. Mann, 'A Practical Recipe for Stable Isotope Labeling by Amino Acids in Cell Culture (Silac)', Nat Protoc, 1 (2006), 2650-60. 41 T. Pawson, and J. D. Scott, 'Protein Phosphorylation in Signaling - 50 Years and Counting', Trends in Biochemical Sciences, 30 (2005), 286-90. 42 T. A. Prime, D. J. Sherrier, P. Mahon, L. C. Packman, and P. Dupree, 'A Proteomic Analysis of Organelles from Arabidopsis Thaliana', Electrophoresis, 21 (2000), 3488-99. 43 Y. J. Qiu, J. Xi, L. Q. Du, S. Roje, and B. W. Poovaiah, 'A Dual Regulatory Role of Arabidopsis Calreticulin-2 in Plant Innate Immunity', Plant Journal, 69 (2012), 489-500. 44 J. Rappsilber, M. Mann, and Y. Ishihama, 'Protocol for Micro-Purification, Enrichment, Pre-Fractionation and Storage of Peptides for Proteomics Using Stagetips', Nat Protoc, 2 (2007), 1896-906. 45 A. Ravichandran, N. Sugiyama, M. Tomita, S. Swarup, and Y. Ishihama, 'Ser/Thr/Tyr Phosphoproteome Analysis of Pathogenic and Non-Pathogenic Pseudomonas Species', Proteomics, 9 (2009), 2764-75. 46 M. C. S. Rodriguez, M. Petersen, and J. Mundy, 'Mitogen-Activated Protein Kinase Signaling in Plants', Annual Review of Plant Biology, Vol 61, 61 (2010), 621-49. 47 P. L. Ross, Y. L. N. Huang, J. N. Marchese, B. Williamson, K. Parker, S. Hattan, N. Khainovski, S. Pillai, S. Dey, S. Daniels, S. Purkayastha, P. Juhasz, S. Martin, M. Bartlet-Jones, F. He, A. Jacobson, and D. J. Pappin, 'Multiplexed Protein Quantitation in Saccharomyces Cerevisiae Using Amine-Reactive Isobaric Tagging Reagents', Molecular & Cellular Proteomics, 3 (2004), 1154-69. 48 H. Saito, Y. Oda, T. Sato, J. Kuromitsu, and Y. Ishihama, 'Multiplexed Two-Dimensional Liquid Chromatography for Maldi and Nanoelectrospray Ionization Mass Spectrometry in Proteomics', Journal of Proteome Research, 5 (2006), 1803-7. 49 A. Sharma, M. Matsuoka, H. Tanaka, and S. Komatsu, 'Antisense Inhibition of a Bri1 Receptor Reveals Additional Protein Kinase Signaling Components Downstream to the Perception of Brassinosteroids in Rice', Febs Letters, 507 (2001), 346-50. 50 P. Sharma, and R. Bhardwaj, 'Effects of 24-Epibrassinolide on Growth and Metal Uptake in Brassica Juncea L. Under Copper Metal Stress', Acta Physiologiae Plantarum, 29 (2007), 259-63. 51 P. Sharma, R. Bhardwaj, N. Arora, H. K. Arora, and A. Kumar, 'Effects of 28-Homobrassinolide on Nickel Uptake, Protein Content and Antioxidative Defence System in Brassica Juncea', Biologia Plantarum, 52 (2008), 767-70. 52 Y. F. Shen, J. M. Jacobs, D. G. Camp, R. H. Fang, R. J. Moore, R. D. Smith, W. Z. Xiao, R. W. Davis, and R. G. Tompkins, 'Ultra-High-Efficiency Strong Cation Exchange Lc/Rplc/Ms/Ms for High Dynamic Range Characterization of the Human Plasma Proteome', Analytical Chemistry, 76 (2004), 1134-44. 53 A. K. Sinha, M. Jaggi, B. Raghuram, and N. Tuteja, 'Mitogen-Activated Protein Kinase Signaling in Plants under Abiotic Stress', Plant Signal Behav, 6 (2011), 196-203. 54 B. Soufi, C. Jers, M. E. Hansen, D. Petranovic, and I. Mijakovic, 'Insights from Site-Specific Phosphoproteomics in Bacteria', Biochimica Et Biophysica Acta-Proteins and Proteomics, 1784 (2008), 186-92. 55 N. Sugiyama, T. Masuda, K. Shinoda, A. Nakamura, M. Tomita, and Y. Ishihama, 'Phosphopeptide Enrichment by Aliphatic Hydroxy Acid-Modified Metal Oxide Chromatography for Nano-Lc-Ms/Ms in Proteomics Applications', Mol Cell Proteomics, 6 (2007), 1103-9. 56 W. Tang, Z. Deng, and Z. Y. Wang, 'Proteomics Shed Light on the Brassinosteroid Signaling Mechanisms', Curr Opin Plant Biol, 13 (2010), 27-33. 57 W. Q. Tang, Z. P. Deng, J. A. Oses-Prieto, N. Suzuki, S. W. Zhu, X. Zhang, A. L. Burlingame, and Z. Y. Wang, 'Proteomics Studies of Brassinosteroid Signal Transduction Using Prefractionation and Two-Dimensional Dige', Molecular & Cellular Proteomics, 7 (2008), 728-38. 58 W. Q. Tang, T. W. Kim, J. A. Oses-Prieto, Y. Sun, Z. P. Deng, S. W. Zhu, R. J. Wang, A. L. Burlingame, and Z. Y. Wang, 'Bsks Mediate Signal Transduction from the Receptor Kinase Bri1 in Arabidopsis', Science, 321 (2008), 557-60. 59 N. L. Taylor, Y. F. Tan, R. P. Jacoby, and A. H. Millar, 'Abiotic Environmental Stress Induced Changes in the Arabidopsis Thaliana Chloroplast, Mitochondria and Peroxisome Proteomes', J Proteomics, 72 (2009), 367-78. 60 H. Thiellement, N. Bahrman, C. Damerval, C. Plomion, M. Rossignol, V. Santoni, D. de Vienne, and M. Zivy, 'Proteomics for Genetic and Physiological Studies in Plants', Electrophoresis, 20 (1999), 2013-26. 61 L. Vercruyssen, N. Gonzalez, T. Werner, T. Schmulling, and D. Inze, 'Combining Enhanced Root and Shoot Growth Reveals Cross Talk between Pathways That Control Plant Organ Size in Arabidopsis', Plant Physiol, 155 (2011), 1339-52. 62 G. Vert, and J. Chory, 'Downstream Nuclear Events in Brassinosteroid Signalling', Nature, 441 (2006), 96-100. 63 A. Vitale, 'Calreticulins Are Not All the Same', Proc Natl Acad Sci U S A, 106 (2009), 13151-2. 64 X. F. Wang, M. B. Goshe, E. J. Soderblom, B. S. Phinney, J. A. Kuchar, J. Li, T. Asami, S. Yoshida, S. C. Huber, and S. D. Clouse, 'Identification and Functional Analysis of in Vivo Phosphorylation Sites of the Arabidopsis Brassinosteroid-Insensitive1 Receptor Kinase', Plant Cell, 17 (2005), 1685-703. 65 M. P. Washburn, D. Wolters, and J. R. Yates, 'Large-Scale Analysis of the Yeast Proteome by Multidimensional Protein Identification Technology', Nature Biotechnology, 19 (2001), 242-47. 66 T. Whelan, W. M. Sackett, and C. R. Benedict, 'Enzymatic Fractionation of Carbon Isotopes by Phosphoenolpyruvate Carboxylase from C4 Plants', Plant Physiol, 51 (1973), 1051-54. 67 E. S. Witze, W. M. Old, K. A. Resing, and N. G. Ahn, 'Mapping Protein Post-Translational Modifications with Mass Spectrometry', Nat Methods, 4 (2007), 798-806. 68 Y. H. Yin, D. Vafeados, Y. Tao, S. Yoshida, T. Asami, and J. Chory, 'A New Class of Transcription Factors Mediates Brassinosteroid-Regulated Gene Expression in Arabidopsis', Cell, 120 (2005), 249-59. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63543 | - |
dc.description.abstract | 油菜固醇是一固醇類植物荷爾蒙,在植物內廣泛的調控生長和發育。油菜固醇的訊息傳遞路徑為細胞表面的激酶受器偵測油菜固醇的訊號後,經由一系列的蛋白質磷酸化與去磷酸化,進行細胞內的訊息傳遞和調控基因表現,進而影響植物的生長與發育。蛋白質磷酸化為一種後轉譯修飾,對細胞內的訊息傳遞和植物的荷爾蒙調節有舉足輕重的影響。目前對於油菜固醇的研究多關注於基因和轉錄體層次的表現,對於磷酸化蛋白質體的相關研究相對少見。本研究目的為探討油菜固醇在植物體內調控的磷酸化蛋白質體。利用一具有根部特性之阿拉伯芥細胞,PSB-D,經油菜固醇處理後,結合雙甲基定量法和二氧化鈦豐富法,並利用液相層析與串聯式質譜儀 (LC-MS/MS)鑑定連續時間點中的磷酸化胜肽及其變化量。在本研究中,共發現了1481條磷酸化胜肽對應519個蛋白質,其中在402個可定量磷酸化胜肽中有12條磷酸化胜肽被判定受到油菜固醇的調控,其包含了核醣體相關蛋白,激酶,磷酸酶等。這些蛋白參與了轉錄,轉譯和生理代謝相關機制,其中可能包含過去未被發現經油菜固醇調控的蛋白質。本研究提供了油菜固醇在植物體所調控的磷酸化蛋白質變化的資訊,並有助於了解植物荷爾蒙在蛋白質磷酸化所扮演的角色 | zh_TW |
dc.description.abstract | Brassinosteroid (BR) is a plant hormone involved in almost every stage of plant development. BR response starts from receptor kinase activation, and relays BR signal from cell surface to the nucleus by phosphorylation-mediated signaling. To date, the signaling pathway of BR has been well established at the gene expression level. However, only little information of phosphoproteins in BR signaling can be available. To investigate the global phosphorylation-regulatory responses in BR signaling, a time-dependent phosphoproteomic study was performed in Arabidopsis cells. Dimethyl labeling and TiO2 enrichment combined with liquid chromatography-tandem mass spectrometry (LC-MS/MS) were used to characterize the phosphoproteome of BR signaling process. Our results identified 519 phosphorylated proteins from 1481 phosphorylated peptides. We further focused on significant change in quantification phosphoprotein. Changed in 12 of 402 quantitated phosphopeptides were significant. These proteins containing ribosome associated proteins, kinases and phosphatases are known to be involved in transcription, translation regulation and metabolic processes. Several proteins that may be regulated by BR were not known previously. This study uncovered a wide-range of phosphorylated proteins in response to BR signaling. Our results not only provide useful information for the phosphoproteomic research in plant hormone, but also reveal a new picture of signal transduction on BR regulation. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T17:13:46Z (GMT). No. of bitstreams: 1 ntu-101-R99B43007-1.pdf: 3689681 bytes, checksum: fd6c350f10af2abfe91d1d645d108074 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 誌謝 i
中文摘要 ii ABSTRACT iii ABBRRVIATION v CONTENTS vii LIST OF FIGURES xi LIST OF TABLES xii CHAPTER 1 INTRODUCTION 1 1.1 Arabidopsis thaliana 1 1.1.1 Global introduction 1 1.1.2 PSB-D cell 1 1.2 Brassinosteroid 2 1.2.1 The structure of brassinosteroid 2 1.2.2 The function of brassinostreoid 3 1.2.3 BR signaling pathway 3 1.3 Proteomics study 4 1.3.1 Mass spectrometry-based proteomics 4 1.3.2 Quantitative proteomes 6 1.3.3 Phosphoproteomic study 6 1.3.4 Proteomic studies of BRs in Arabidopsis 8 1.4 Motivation 9 CHAPTER 2 MATERIALS AND METHODS 11 2.1 Plant materials and growth conditions 11 2.1.1 Arabidopsis thaliana 11 2.1.2 PSB-D cell line 11 2.1.3 Brassinosteroid treatment 12 2.2 Real-time PCR 12 2.3 StageTips preparation 12 2.3.1 C8 StageTips 12 2.3.2 SDB-XC StageTips 13 2.4 Phosphoproteome sample preparation 14 2.4.1 Protein reduction, alkylation and digestion 14 2.4.2 Dimethyl labeling 15 2.4.3 Desalting with C18 StageTips 16 2.4.4 Enrichment of phosphopeptides with HAMMOC 16 2.4.5 NanoLC-MS/MS analysis 17 2.5 Database searching 18 CHAPTER 3 RESULTS 20 3.1 The plant morphology of BR treatment 20 3.2 Expression of BR-regulated genes 21 3.3 BR-regulated phosphoproteome profile 22 3.3.1 An overview of BR-regulated phosphoproteome 22 3.3.2 The quantitative analysis of phosphoproteome 24 CHAPTER 4 DISCUSSION 26 4.1 BR-regulated gene expression 26 4.2 Identified protein coverage in biological replicates 27 4.3 Significant profile change in BR-regulated 28 4.3.1 Phosphoenolpyruvate carboxylase 28 4.3.2 Calreticulin 2 (CRT2) 29 4.3.3 Fructose-2,6-bisphosphate 2-phosphatase 29 4.3.4 Mitogen-activated protein kinase 30 CHAPTER 5 CONCLUSION 32 CHAPTER 6 FUTURE WORKS 33 References 34 Figures 47 Tables 64 Appendix 105 | |
dc.language.iso | en | |
dc.title | 油菜固醇調控阿拉伯芥之磷酸化蛋白質體研究 | zh_TW |
dc.title | Brassinosteroid-regulated Phosphoproteome in Arabidopsis thaliana | en |
dc.type | Thesis | |
dc.date.schoolyear | 100-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃宣誠,吳世雄,李文雄,謝旭亮 | |
dc.subject.keyword | 油菜固醇,阿拉伯芥,磷酸化蛋白質體,蛋白質體定量分析, | zh_TW |
dc.subject.keyword | Brassinosteroid,Arabidopsis thaliana,Phosphoproteome,Quantitative proteome, | en |
dc.relation.page | 106 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2012-08-20 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 分子與細胞生物學研究所 | zh_TW |
顯示於系所單位: | 分子與細胞生物學研究所 |
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