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DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭尊仁 | |
dc.contributor.author | Cheng-Kuan Wu | en |
dc.contributor.author | 胡靜君 | zh_TW |
dc.date.accessioned | 2021-05-16T16:26:47Z | - |
dc.date.available | 2015-03-04 | |
dc.date.available | 2021-05-16T16:26:47Z | - |
dc.date.copyright | 2013-03-04 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-02-05 | |
dc.identifier.citation | 1. Sung JH, Ji JH, Park JD, Yoon JU, Kim DS, Jeon KS, Song MY, Jeong J, Han BS, Han JH, et al: Subchronic Inhalation Toxicity of Silver Nanoparticles. Toxicological Sciences 2009, 108:452-461.
2. Ji JH, Jung JH, Kim SS, Yoon JU, Park JD, Choi BS, Chung YH, Kwon IH, Jeong J, Han BS, et al: Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhal Toxicol 2007, 19:857-871. 3. Kim YS, Song MY, Park JD, Song KS, Ryu HR, Chung YH, Chang HK, Lee JH, Oh KH, Kelman BJ, et al: Subchronic oral toxicity of silver nanoparticles. Particle and Fibre Toxicology 2010, 7. 4. Stebounova LV, Adamcakova-Dodd A, Kim JS, Park H, O'Shaughnessy PT, Grassian VH, Thorne PS: Nanosilver induces minimal lung toxicity or inflammation in a subacute murine inhalation model. Part Fibre Toxicol 2011, 8:5. 5. Oberdorster G, Sharp Z, Atudorei V, Elder A, Gelein R, Kreyling W, Cox C: Translocation of inhaled ultrafine particles to the brain. Inhalation Toxicology 2004, 16:437-445. 6. Wijnhoven SWP, Peijnenburg WJGM, Herberts CA, Hagens WI, Oomen AG, Heugens EHW, Roszek B, Bisschops J, Gosens I, Van De Meent D, et al: Nano-silver – a review of available data and knowledge gaps in human and environmental risk assessment. Nanotoxicology 2009, 3:109-138. 7. Scheringer M, MacLeod M, Behra R, Sigg L, Hungerbuhler K: Environmental risks associated with nanoparticulate silver used as biocide, Household and Personal Care Today. Household Pers Care Today 2010, 1:34-37. 8. Nowack B: Nanosilver Revisited Downstream. Science 2010, 330:1054-1055. 9. Donaldson K, Li XY, Macnee W: Ultrafine (nanometre) particle mediated lung injury. Journal of Aerosol Science 1998, 29:553-560. 10. Kreyling WG, Semmler M, Erbe F, Mayer P, Takenaka S, Schulz H, Oberdorster G, Ziesenis A: Translocation of ultrafine insoluble iridium particles from lung epithelium to extrapulmonary organs is size dependent but very low. Journal of Toxicology and Environmental Health-Part A 2002, 65:1513-1530. 11. Ferin J, Oberdorster G, Penney DP: Pulmonary Retention of Ultrafine and Fine Particles in Rats. American Journal of Respiratory Cell and Molecular Biology 1992, 6:535-542. 12. Campbell A, Oldham M, Becaria A, Bondy SC, Meacher D, Sioutas C, Misra C, Mendez LB, Kleinman A: Particulate matter in polluted air may increase biomarkers of inflammation in mouse brain. Neurotoxicology 2005, 26:133-140. 13. Lea MC: On Allotropic Forms Of Silver. Am J Sci 1889, 37:476-491. 14. Gottschalk F, Scholz RW, Nowack B: Probabilistic material flow modeling for assessing the environmental exposure to compounds: Methodology and an application to engineered nano-TiO2 particles. Environmental Modelling & Software 2010, 25:320-332. 15. Nowack B, Krug HF, Height M: 120 Years of Nanosilver History: Implications for Policy Makers. Environmental Science & Technology 2011, 45:1177-1183. 16. Zhang H, Smith JA, Oyanedel-Craver V: The effect of natural water conditions on the anti-bacterial performance and stability of silver nanoparticles capped with different polymers. Water Res 2012, 46:691-699. 17. Zhu X, Bai R, Wee K-H, Liu C, Tang S-L: Membrane surfaces immobilized with ionic or reduced silver and their anti-biofouling performances. Journal of Membrane Science 2010, 363:278-286. 18. Chen Y, Zheng X, Xie Y, Ding C, Ruan H, Fan C: Anti-bacterial and cytotoxic properties of plasma sprayed silver-containing HA coatings. J Mater Sci Mater Med 2008, 19:3603-3609. 19. Lee JH, Mun J, Park JD, Yu IJ: A health surveillance case study on workers who manufacture silver nanomaterials. Nanotoxicology 2012, 6:667-669. 20. Lee JH, Kwon M, Ji JH, Kang CS, Ahn KH, Han JH, Yu IJ: Exposure assessment of workplaces manufacturing nanosized TiO2 and silver. Inhalation Toxicology 2011, 23:226-236. 21. Miller A, Drake PL, Hintz P, Habjan M: Characterizing exposures to airborne metals and nanoparticle emissions in a refinery. Ann Occup Hyg 2010, 54:504-513. 22. 張振平, 陳春萬: 不同型態奈米微粒之製作與細胞株或動物暴露系統開發與測試研究. 2009. 23. Braydich-Stolle L, Hussain S, Schlager JJ, Hofmann MC: In vitro cytotoxicity of nanoparticles in mammalian germline stem cells. Toxicological Sciences 2005, 88:412-419. 24. Hussain SM, Hess KL, Gearhart JM, Geiss KT, Schlager JJ: In vitro toxicity of nanoparticles in BRL 3A rat liver cells. Toxicology in Vitro 2005, 19:975-983. 25. Carlson C, Hussain SM, Schrand AM, Braydich-Stolle LK, Hess KL, Jones RL, Schlager JJ: Unique Cellular Interaction of Silver Nanoparticles: Size-Dependent Generation of Reactive Oxygen Species. Journal of Physical Chemistry B 2008, 112:13608-13619. 26. Arora S, Jain J, Rajwade JM, Paknikar KM: Interactions of silver nanoparticles with primary mouse fibroblasts and liver cells. Toxicol Appl Pharmacol 2009, 236:310-318. 27. Hsin YH, Chena CF, Huang S, Shih TS, Lai PS, Chueh PJ: The apoptotic effect of nanosilver is mediated by a ROS- and JNK-dependent mechanism involving the mitochondrial pathway in NIH3T3 cells. Toxicology Letters 2008, 179:130-139. 28. Park EJ, Yi J, Kim Y, Choi K, Park K: Silver nanoparticles induce cytotoxicity by a Trojan-horse type mechanism. Toxicology in Vitro 2010, 24:872-878. 29. Kim YS, Kim JS, Cho HS, Rha DS, Kim JM, Park JD, Choi BS, Lim R, Chang HK, Chung YH, et al: Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 2008, 20:575-583. 30. Jeong GN, Jo UB, Ryu HY, Kim YS, Song KS, Yu IJ: Histochemical study of intestinal mucins after administration of silver nanoparticles in Sprague-Dawley rats. Archives of Toxicology 2010, 84:63-69. 31. Park EJ, Bae E, Yi J, Kim Y, Choi K, Lee SH, Yoon J, Lee BC, Park K: Repeated-dose toxicity and inflammatory responses in mice by oral administration of silver nanoparticles. Environmental Toxicology and Pharmacology 2010, 30:162-168. 32. Quadros ME, Marr LC: Environmental and Human Health Risks of Aerosolized Silver Nanoparticles. Journal of the Air & Waste Management Association 2010, 60:770-781. 33. Oka Y, Mitsui M, Kitahashi T, Sakamoto A, Kusuoka O, Tsunoda T, Mori T, Tsutsumi M: A Reliable Method for Intratracheal Instillation of Materials to the Entire Lung in Rats. Journal of Toxicologic Pathology 2006, 19:107-109. 34. Driscoll KE, Costa DL, Hatch G, Henderson R, Oberdorster G, Salem H, Schlesinger RB: Intratracheal instillation as an exposure technique for the evaluation of respiratory tract toxicity: Uses and limitations. Toxicological Sciences 2000, 55:24-35. 35. Leong BKJ, Coombs JK, Sabaitis CP, Rop DA, Aaron CS: Quantitative morphometric analysis of pulmonary deposition of aerosol particles inhaled via intratracheal nebulization, intratracheal instillation or nose-only inhalation in rats. Journal of Applied Toxicology 1998, 18:149-160. 36. Park EJ, Choi K, Park K: Induction of Inflammatory Responses and Gene Expression by Intratracheal Instillation of Silver Nanoparticles in Mice. Archives of Pharmacal Research 2011, 34:299-307. 37. Takenaka S, Karg E, Roth C, Schulz H, Ziesenis A, Heinzmann U, Schramel P, Heyder J: Pulmonary and Systemic Distribution of Inhaled Ultrafine Silver Particles in Rats. Environmental Health Perspectives 2001, 109:547-551. 38. Gumbleton M: Caveolae as potential macromolecule trafficking compartments within alveolar epithelium. Advanced Drug Delivery Reviews 2001, 49:281-300. 39. Yu LE, Yung LYL, Ong CN, Tan YL, Balasubramaniam KS, Hartono D, Shui GH, Wenk MR, Ong WY: Translocation and effects of gold nanoparticles after inhalation exposure in rats. Nanotoxicology 2007, 1:235-242. 40. Sung JH, Ji JH, Song KS, Lee JH, Choi KH, Lee SH, Yu IJ: Acute inhalation toxicity of silver nanoparticles. Toxicol Ind Health 2011, 27:149-154. 41. Ji JH, Jung JH, Kim SS, Yoon JU, Park JD, Choi BS, Chung YH, Kwon IH, Jeong J, Han BS, et al: Twenty-eight-day inhalation toxicity study of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicology 2007, 19:857-871. 42. J. L, A. F, Immunology. ftEAoAaC: Allergy: an epidemic that must be stopped. Brussels: European Academy of Allergology and Clinical Immunology 2006. 43. 吳家興, 林瑞雄, 謝貴雄: 台灣北部國中學生氣喘盛行率調查. 中華衛誌 1998, 17:214-225. 44. Weinmayr G, Weiland SK, Bjorksten B, Brunekreef B, Buchele G, Cookson WO, Garcia-Marcos L, Gotua M, Gratziou C, van Hage M, et al: Atopic sensitization and the international variation of asthma symptom prevalence in children. Am J Respir Crit Care Med 2007, 176:565-574. 45. Yazdanbakhsh M, Kremsner PG, van Ree R: Allergy, parasites, and the hygiene hypothesis. Science 2002, 296:490-494. 46. Secrist H, Chelen CJ, Wen Y, Marshall JD, Umetsu DT: Allergen immunotherapy decreases interleukin 4 production in CD4+ T cells from allergic individuals. The Journal of Experimental Medicine 1993, 178:2123-2130. 47. Busse WW, Lemanske RF, Jr.: Asthma. N Engl J Med 2001, 344:350-362. 48. von Klot S, Wolke G, Tuch T, Heinrich J, Dockery DW, Schwartz J, Kreyling WG, Wichmann HE, Peters A: Increased asthma medication use in association with ambient fine and ultrafine particles. European Respiratory Journal 2002, 20:691-702. 49. Wichmann HF, Spix C, Tuch T, Wolke G, Peters A, Heinrich J, Kreyling WG, Heyder J: Daily mortality and fine and ultrafine particles in Erfurt, Germany. Part I: role of particle number and particle mass. Rep Health Eff Inst 2000, 98:5-86. 50. Boulet LP, Lemiere C, Gautrin D, Cartier A: New insights into occupational asthma. Curr Opin Allergy Clin Immunol 2007, 7:96-101. 51. Bello D, Herrick CA, Smith TJ, Woskie SR, Streicher RP, Cullen MR, Liu YC, Redlich CA: Skin exposure to isocyanates: Reasons for concern. Environmental Health Perspectives 2007, 115:328-335. 52. Hussain S, Vanoirbeek JAJ, Luyts K, De Vooght V, Verbeken E, Thomassen LCJ, Martens JA, Dinsdale D, Boland S, Marano F, et al: Lung exposure to nanoparticles modulates an asthmatic response in a mouse model. European Respiratory Journal 2011, 37:299-309. 53. Park HS, Kim KH, Jang S, Park JW, Cha HR, Lee JE, Kim JO, Kim SY, Lee CS, Kim JP, Jung SS: Attenuation of allergic airway inflammation and hyperresponsiveness in a murine model of asthma by silver nanoparticles. International journal of nanomedicine 2010, 5:505-515. 54. Jang S, Park JW, Cha HR, Jung SY, Lee JE, Jung SS, Kim JO, Kim SY, Lee CS, Park HS: Silver nanoparticles modify VEGF signaling pathway and mucus hypersecretion in allergic airway inflammation. Int J Nanomedicine 2012, 7:1329-1343. 55. 鄭福田 李林: 可控制奈米微粒產生技術開發. 勞工安全衛生研究季刊 2009, 第17卷:第153-162頁. 56. Jung JH, Oh HC, Noh HS, Ji JH, Kim SS: Metal nanoparticle generation using a small ceramic heater with a local heating area. Journal of Aerosol Science 2006, 37:1662-1670. 57. Ji JH, Jung JH, Yu IJ, Kim SS: Long-term stability characteristics of metal nanoparticle generator using small ceramic heater for inhalation toxicity studies. Inhalation Toxicology 2007, 19:745-751. 58. Demokritou P, Buchel R, Molina RM, Deloid GM, Brain JD, Pratsinis SE: Development and characterization of a Versatile Engineered Nanomaterial Generation System (VENGES) suitable for toxicological studies. Inhalation Toxicology 2010, 22:107-116. 59. Teleki A, Wengeler R, Wengeler L, Nirschl H, Pratsinis SE: Distinguishing between aggregates and agglomerates of flame-made TiO2 by high-pressure dispersion. Powder Technology 2008, 181:292-300. 60. Madler L, Pratsinis SE: Bismuth Oxide Nanoparticles by Flame Spray Pyrolysis. Journal of the American Ceramic Society 2002, 85:1713-1718. 61. Madler L, Kammler HK, Mueller R, Pratsinis SE: Controlled synthesis of nanostructured particles by flame spray pyrolysis. Journal of Aerosol Science 2002, 33:369-389. 62. Shimada M, Wang W-N, Okuyama K, Myojo T, Oyabu T, Morimoto Y, Tanaka I, Endoh S, Uchida K, Ehara K, et al: Development and Evaluation of an Aerosol Generation and Supplying System for Inhalation Experiments of Manufactured Nanoparticles. Environmental Science & Technology 2009, 43:5529-5534. 63. Szefler SJ, Wenzel S, Brown R, Erzurum SC, Fahy JV, Hamilton RG, Hunt JF, Kita H, Liu AH, Panettieri RA, et al: Asthma outcomes: Biomarkers. Journal of Allergy and Clinical Immunology 2012, 129:S9-S23. 64. 游妙娟: 奈米氧化鋅微粒於自發性高血壓大鼠之呼吸毒理研究. 國立台灣大學職業醫學與工業衛生研究所碩士論文 2008. 65. Gueders MM, Paulissen G, Crahay C, Quesada-Calvo F, Hacha J, Van Hove C, Tournoy K, Louis R, Foidart JM, Noel A, Cataldo DD: Mouse models of asthma: a comparison between C57BL/6 and BALB/c strains regarding bronchial responsiveness, inflammation, and cytokine production. Inflamm Res 2009, 58:845-854. 66. Resolution of bronchial hyperresponsiveness.pdf 67. A Murine IL-4 Receptor Antagonist That Inhibits IL-4- and IL-13-Induced Responses Prevents Antigen-Induced Airway Eosinophilia and Airway Hyperresponsiveness.pdf 68. Airway Subepithelial Fibrosis in a Murine Model of Atopic Asthma.pdf 69. Rossi E, Pylkkanen L, Koivisto A, Nykasenoja H, Wolff H, Savolainen K, Alenius H: Inhalation exposure to nanosized and fine TiO2 particles inhibits features of allergic asthma in a murine model. Particle and Fibre Toxicology 2010, 7:35. 70. Sioutas C: Evaluation of the Measurement Performance of the Scanning Mobility Particle Sizer and Aerodynamic Particle Sizer. Aerosol Science and Technology 1999, 30:84-92. 71. Ho M, Wu KY, Chein HM, Chen LC, Cheng TJ: Pulmonary toxicity of inhaled nanoscale and fine zinc oxide particles: mass and surface area as an exposure metric. Inhal Toxicol 2011, 23:947-956. 72. Kim SC, Wang J, Emery MS, Shin WG, Mulholland GW, Pui DYH: Structural Property Effect of Nanoparticle Agglomerates on Particle Penetration through Fibrous Filter. Aerosol Science and Technology 2009, 43:344-355. 73. Liu Z, Kim SC, Wang J, Shin WG, Fissan H, Pui DYH: Measurement of Metal Nanoparticle Agglomerates Generated by Spark Discharge Using the Universal Nanoparticle Analyzer (UNPA). Aerosol Science and Technology 2012, 46:333-346. 74. Sayes CM, Reed KL, Warheit DB: Assessing toxicity of fine and nanoparticles: comparing in vitro measurements to in vivo pulmonary toxicity profiles. Toxicol Sci 2007, 97:163-180. 75. Cho WS, Duffin R, Poland CA, Duschl A, Oostingh GJ, Macnee W, Bradley M, Megson IL, Donaldson K: Differential pro-inflammatory effects of metal oxide nanoparticles and their soluble ions in vitro and in vivo; zinc and copper nanoparticles, but not their ions, recruit eosinophils to the lungs. Nanotoxicology 2012, 6:22-35. 76. Sung JH, Ji JH, Yoon JU, Kim DS, Song MY, Jeong J, Han BS, Han JH, Chung YH, Kim J, et al: Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhal Toxicol 2008, 20:567-574. 77. Hyun JS, Lee BS, Ryu HY, Sung JH, Chung KH, Yu IJ: Effects of repeated silver nanoparticles exposure on the histological structure and mucins of nasal respiratory mucosa in rats. Toxicology Letters 2008, 182:24-28. 78. Hardy CL, LeMasurier JS, Belz GT, Scalzo-Inguanti K, Yao J, Xiang SD, Kanellakis P, Bobik A, Strickland DH, Rolland JM, et al: Inert 50-nm polystyrene nanoparticles that modify pulmonary dendritic cell function and inhibit allergic airway inflammation. J Immunol 2012, 188:1431-1441. 79. Cho WS, Duffin R, Howie SE, Scotton CJ, Wallace WA, Macnee W, Bradley M, Megson IL, Donaldson K: Progressive severe lung injury by zinc oxide nanoparticles; the role of Zn2+ dissolution inside lysosomes. Part Fibre Toxicol 2011, 8:27. 80. <Anti-inflammatory effect of topical.pdf>. 81. Bhol KC, Schechter PJ: Topical nanocrystalline silver cream suppresses inflammatory cytokines and induces apoptosis of inflammatory cells in a murine model of allergic contact dermatitis. Br J Dermatol 2005, 152:1235-1242. 82. Hussain S, Boland S, Baeza-Squiban A, Hamel R, Thomassen LC, Martens JA, Billon-Galland MA, Fleury-Feith J, Moisan F, Pairon JC, Marano F: Oxidative stress and proinflammatory effects of carbon black and titanium dioxide nanoparticles: role of particle surface area and internalized amount. Toxicology 2009, 260:142-149. 83. Murphy SA, BeruBe KA, Pooley FD, Richards RJ: The response of lung epithelium to well characterised fine particles. Life Sci 1998, 62:1789-1799. 84. 王友正: 研究纳美芬與纳美芬酮之抗發炎效果以減緩氣喘之呼吸道發炎反應. 臺灣大學口腔生物科學研究所學位論文 2009. 85. Pichavant M, Goya S, Hamelmann E, Gelfand EW, Umetsu DT: Animal models of airway sensitization. Curr Protoc Immunol 2007, Chapter 15:Unit 15 18. 86. Nials AT, Uddin S: Mouse models of allergic asthma: acute and chronic allergen challenge. Dis Model Mech 2008, 1:213-220. 87. Cha K, Hong HW, Choi YG, Lee MJ, Park JH, Chae HK, Ryu G, Myung H: Comparison of acute responses of mice livers to short-term exposure to nano-sized or micro-sized silver particles. Biotechnol Lett 2008, 30:1893-1899. 88. Leigh R, Ellis R, Wattie J, Southam DS, de Hoogh M, Gauldie J, O'Byrne PM, Inman MD: Dysfunction and remodeling of the mouse airway persist after resolution of acute allergen-induced airway inflammation. American Journal of Respiratory Cell and Molecular Biology 2002, 27:526-535. 89. Johnson JR, Wiley RE, Fattouh R, Swirski FK, Gajewska BU, Coyle AJ, Gutierrez-Ramos JC, Ellis R, Inman MD, Jordana M: Continuous exposure to house dust mite elicits chronic airway inflammation and structural remodeling. Am J Respir Crit Care Med 2004, 169:378-385. 90. Lee SY, Kim JS, Lee JM, Kwon SS, Kim KH, Moon HS, Song JS, Park SH, Kim YK: Inhaled corticosteroid prevents the thickening of airway smooth muscle in murine model of chronic asthma. Pulm Pharmacol Ther 2008, 21:14-19. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/6349 | - |
dc.description.abstract | 由於全球各地的氣喘盛行率逐年持續上升,加上近年奈米科技產品日新月異,尤其以具有抗菌功效的奈米銀應用至為廣泛,因此大量勞工可能在生產過程中吸入空氣中的奈米銀微粒;除了關注奈米銀對一般健康勞工的呼吸毒性外,針對氣喘此易感族群的相關呼吸毒理研究也有其重要性。
本研究利用蒸氣冷凝法產生奈米銀微粒,以進行氣喘動物之呼吸暴露實驗。一開始要先建立氣喘動物模式,將BALB/c小鼠分為健康(PBS)和氣喘模式(OVA)兩組,再細分為奈米銀暴露組(NP)和過濾空氣控制組(FA)。因此四組分別表示為PBS/FA (N=5)、PBS/NP (N=6)、OVA/FA (N=5)、OVA/NP (N=5),暴露時間為6小時/天,連續7天,結束後測量各組呼吸道阻力。最後分析氣喘疾病及肺部發炎指標,以及肺部病理組織切片。 奈米銀暴露組所暴露之奈米微粒平均粒徑為32.78nm,質量濃度為3.34 mg/m3,經過連續7天的暴露後,可觀察到在健康組間,分別在肺泡灌洗液 (BALF)的嗜酸性白血球比率、嗜中性白血球比率及總死亡細胞數三個生理指標中,PBS/NP相比PBS/FA皆有上升趨勢;而在氣喘組間,則在血清的免疫球蛋白E(IgE)(呼吸暴露後)、BALF的介白素13 (IL-13)和總死亡細胞數(total cell),以及呼吸道阻力(AHR)這些生理指標中,OVA/NP 相比OVA/FA皆有上升趨勢。而且肺部病理組織切片的結果也顯示,無論是健康組還是氣喘組,經過奈米銀暴露後的發炎反應會較強烈。 目前針對氣喘疾病的奈米銀呼吸毒理研究非常缺乏,本研究使用由奈米微粒產生器所產生之奈米銀氣膠進行呼吸暴露,可觀察到奈米銀對健康或氣喘小鼠皆有不良健康效應之趨勢,惟此趨勢在統計上並不顯著,推估是由於此氣喘模式中致敏劑劑量過高所致,建議應下調劑量以適用於奈米呼吸暴露研究;此外,過去亦有研究顯示奈米銀微粒對氣喘小鼠反而有抑制發炎的作用,因此目前奈米銀微粒對於氣喘模式小鼠的健康效應未有一致結論,其毒理影響及作用機轉仍有待更多相關研究。 | zh_TW |
dc.description.abstract | Nowadays, asthma is one of the most common airway inflammatory disease and its prevalence is high in developed and developing countries. A wide range of silver nanoparticle applications has emerged in consumer products ranging from disinfecting medical devices to water treatment. We are interested in the inhalation toxicity of silver nanoparticles in susceptible allergic asthmatic mice.
In this study, silver nanoparticles were produced in a generation system. The BALB/c mice were divided into normal group (PBS) and asthmatic group (induced by OVA injection). These two groups were further exposed for filtered air (FA) (PBS/FA (N=5)、OVA/FA (N=5)) and silver nanoparticles (NP) (PBS/NP (N=6)、OVA/NP (N=5)) 6 hours a day for one week. The average size and concentration of NP during exposure was 32.78 nm at 3.34 mg/m3. The inflammation and asthma markers including IgE in serum, IL-13, total protein, total cells, proportion of neutrophils, macrophages and eosinophils in bronchoalveolar lavage fluid (BALF) and AHR (airway hyperresponsiveness) were determined. The results showed that animals had adverse health effects after exposure to AgNPs (silver nanoparticles). In normal group, percentage of neutrophils, eosinophils and total cells in BALF increased after AgNPs exposure as compared to FA control. In asthma group, IgE, IL-13, AHR and total cells also increased after AgNPs exposure as compared to FA control but it was not statistically significant. These results suggested that asthmatic model needs further modification. Additionally, some previous experiments showed that AgNPs might act as anti-inflammatory agents. Thus,further studies are needed to assess the toxicity of AgNPs in allergic asthma model. | en |
dc.description.provenance | Made available in DSpace on 2021-05-16T16:26:47Z (GMT). No. of bitstreams: 1 ntu-102-R99844017-1.pdf: 2918587 bytes, checksum: 825bd8c90fc14f0b09958609961196c9 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 摘要 1
Abstract 3 第一章 前言 5 第二章 背景與重要性 6 2.1 奈米微粒特性與毒性 6 2.2 奈米銀微粒 7 2.2.1 微粒特性 7 2.2.2 流行病學研究 7 2.2.3毒理研究 8 2.3 氣喘 10 2.3.1 氣喘疾病 10 2.3.2 氣喘疾病產生機制 11 2.4奈米產生系統 13 2.5 肺部發炎與傷害指標 15 2.6 研究目的 16 第三章 材料與方法 17 3.1 研究架構及時程 17 3.2 氣喘模式建立 18 3.3呼吸暴露 18 3.3.1 奈米微粒特性檢驗 19 3.4 呼吸道阻力測試 20 3.5 肺泡灌洗液 21 3.5.1肺泡灌洗液中總死亡細胞數 22 3.5.2肺泡灌洗液中血球分類計數 22 3.5.3肺泡灌洗液中細胞因子IL-13 23 3.5.4肺泡灌洗液中總蛋白數 24 3.6血液中的IgE 抗體濃度 24 3.7組織病理切片 25 3.8 統計分析 26 第四章 結果 27 4.1微粒產生暴露系統 27 4.2奈米微粒成分分析 27 4.3 氣喘疾病生理指標 28 4.4 呼吸道阻力 29 4.5 肺部發炎指標 29 4.6組織病理切片 30 第五章 討論與建議 31 5.1 奈米微粒產生及暴露系統 31 5.2 氣喘疾病及肺部發炎指標 34 5.2.1 奈米銀微粒對健康小鼠之呼吸毒理作用 34 5.2.2奈米銀微粒對氣喘小鼠之呼吸毒理作用 35 5.3 組織病理切片 37 5.4肺泡灌洗液中總死亡細胞數及血球分類之計數 37 5.5結論及建議 38 第六章 參考文獻 39 | |
dc.language.iso | zh-TW | |
dc.title | 奈米銀微粒對氣喘模式小鼠之呼吸毒理研究 | zh_TW |
dc.title | Inhalation Toxicity of Silver Nanoparticles in a Murine Model of Allergic Asthma | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 碩士 | |
dc.contributor.coadvisor | 王根樹 | |
dc.contributor.oralexamcommittee | 蕭大智,林靖愉,李珍珍 | |
dc.subject.keyword | 奈米銀微粒,氣喘,蒸發冷凝法,呼吸暴露, | zh_TW |
dc.subject.keyword | silver nanoparticles,asthma,evaporation-condensation,inhalation exposure, | en |
dc.relation.page | 69 | |
dc.rights.note | 同意授權(全球公開) | |
dc.date.accepted | 2013-02-05 | |
dc.contributor.author-college | 公共衛生學院 | zh_TW |
dc.contributor.author-dept | 環境衛生研究所 | zh_TW |
顯示於系所單位: | 環境衛生研究所 |
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