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| DC 欄位 | 值 | 語言 |
|---|---|---|
| dc.contributor.advisor | 蘇慧敏(Hui-Min Su) | |
| dc.contributor.author | Sheng-Hsia Chou | en |
| dc.contributor.author | 周聖夏 | zh_TW |
| dc.date.accessioned | 2021-06-16T16:26:29Z | - |
| dc.date.available | 2018-03-04 | |
| dc.date.copyright | 2013-03-04 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-01-20 | |
| dc.identifier.citation | Armario A, Gavalda A, Marti J (1995) Comparison of the behavioural and endocrine response to forced swimming stress in five inbred strains of rats. Psychoneuroendocrinology 20:879-890.
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| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63176 | - |
| dc.description.abstract | 本論文主要探討飲食中缺乏n-3多元不飽和脂肪酸對大鼠腦區二十二碳六烯酸含量及經由壓力誘發之神經內分泌路徑─下視丘-腦下垂體-腎上腺路徑反應的影響。
利用Sprague-Dawley雄性大鼠，在離乳之後給予n-3多元不飽和脂肪酸缺乏或正常的飼料7週。觀察其腦區二十二碳六烯酸含量的變化，並以行為實驗檢驗兩組大鼠在焦慮及憂鬱相關行為的表現。透過給予大鼠束縛壓力1小時後，檢測其下視丘-腦下垂體-腎上腺路徑的反應。大鼠於犧牲後分析各腦區DHA含量、血清中糖皮質類固醇的含量、糖皮質類固醇受體蛋白及brain-derived neurotrophic factor (BDNF)蛋白在海馬及下視丘的表現。實驗結果發現在離乳後攝取缺乏n-3多元不飽和脂肪酸飼料的大鼠，不論在海馬、下視丘、前額葉皮質、杏仁核、腦幹及肝臟，二十二碳六烯酸的含量均較正常組顯著地降低。然而在焦慮或憂鬱相關行為的檢測上兩組並無差異。在給予束縛壓力後，血中糖皮質類固醇的濃度在攝取n-3多元不飽和脂肪酸缺乏飼料的組別明顯較高。但在海馬及下視丘的糖皮質類固醇受體蛋白表現量並無差異，而BDNF蛋白於海馬的表現量在壓力後，缺乏n-3多元不飽和脂肪酸的組別會較正常組別下降。綜合以上，本論文主要發現雄性大鼠離乳後，攝取缺乏n-3多元不飽和脂肪酸飼料7周會造成各腦區二十二碳六烯酸含量顯著下降，及增加由壓力誘發的糖皮質固醇分泌。 | zh_TW |
| dc.description.abstract | Most previous studies have focused on rat docosahexaenoic acid (22:6n-3, DHA) deficiency created during brain development via maternal n-3 fatty acid-deficient diet from pregnancy and lactation on adult anxiety and depression behaviors. It has been suggested the anxiety and depression is associated with hypothalamic-pituitary-adrenal axis (HPA axis) responses. The aim of this study was to evaluate whether brain DHA deficiency during post-weaning period would enhance restrain-induced HPA axis responses and anxiety or depression-like behavior in adult male rats. After weaning at postnatal day 21, male rats fed an n-3 fatty acid-deficient or an n-3 fatty acid-adequate diet till sacrificed at postnatal day 70. The DHA levels were significant decreased in hypothalamus and hippocampus and other brain regions in rats fed with n-3 fatty acid-deficient diet compared to the rats fed with n-3 fatty acid-adequate diet during post-weaning period for 7 weeks. The anxiety-like behavior in the elevated plus-maze test and depressive-like behavior in the forced swim test were no difference between groups. The restraint-induced rectal temperature was increased during the restraint, but no difference between groups. The serum corticosterone was significant increased right after restraint in rats fed an n-3 fatty acid-deficient diet. The GR expression in hippocampus and hypothalamus were no difference between groups. The BDNF was significant decreased in hippocampus after restraint in rats fed n-3 fatty acid-deficient diet compared to the rats fed n-3 fatty acid-adequate diet. This study suggest that brain DHA level can be decreased in male rats fed n-3 fatty acid-deficient diet during post-weaning period, and the decreased brain DHA is associated with higher stress-induced serum corticosterone levels. | en |
| dc.description.provenance | Made available in DSpace on 2021-06-16T16:26:29Z (GMT). No. of bitstreams: 1 ntu-102-R99441009-1.pdf: 2368418 bytes, checksum: 6cf17bd5630db1f8a3ed7d197bc3b55a (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 中文摘要 I
英文摘要 II 圖目錄 VI 表目錄 VIII 第壹章、文獻回顧 1 第一節、二十二碳六烯酸 1 第二節、必需脂肪酸的生合成 2 第三節、二十二碳六烯酸與憂鬱症 3 第四節、下視丘-腦下垂體-腎上腺路徑 4 第五節、HPA axis與憂鬱症 5 第六節、壓力模式的選擇 7 第七節、缺乏DHA的動物模式 9 第貳章、實驗目的與假設 11 第參章、材料與方法 12 一、 實驗設計 12 二、 實驗用飼料配方與分析 13 1. 自製實驗飼料組成 14 2. 飼料脂肪酸組成比較 15 三、 行為實驗 16 1. 抬高型十字迷宮實驗 (Elevated plus-maze) 16 2. 強迫游泳實驗(Forced swim test) 17 四、 束縛壓力實驗(Restraint stress) 18 五、 動物犧牲與組織收集 19 六、 脂肪酸檢測分析 19 1. 藥品來源 19 2. 分析流程 20 七、 糖皮質類固醇酵素免疫分析 22 八、 腦組織中GR及BDNF蛋白表現分析 24 1. 蛋白質萃取 24 2. 蛋白質定量 25 3. 電泳檢定-西方墨點法 (Western blot) 25 4. 轉印 27 5. 酵素免疫染色法 28 6. 冷光照相與定量 29 九、 統計分析 29 第肆章、實驗結果 30 一、 脂肪酸組成分析 30 二、 動物焦慮程度之檢測 37 三、 動物憂鬱程度之檢測 38 四、 動物經束縛壓力之肛溫變化 39 五、 血清中corticosterone之測量 41 六、 海馬及下視丘中GR蛋白之表現 42 七、 海馬及下視丘中BDNF蛋白之表現 43 第伍章、討論 45 一、 動物模式的選擇 45 二、 離乳後缺乏n-3 PUFA對大鼠DHA含量的影響 46 三、 離乳後缺乏n-3 PUFA對大鼠情緒的影響 49 四、 離乳後缺乏n-3 PUFA對大鼠遭遇壓力之反應的影響 50 五、 離乳後缺乏n-3 PUFA對大鼠海馬及下視丘GR表現的影響 52 第陸章、總結 54 Appendix 55 1. 初始實驗設計及動物數量分配 55 1-1. 實驗設計 55 1-2. 實驗動物數量分配 56 2. 大鼠出生後體重及10周大雄性大鼠體重及腦重 57 3. 十字迷宮實驗 58 4. 強迫游泳實驗 59 5. 動物經束縛壓力之肛溫變化 60 6. 血清中corticosterone濃度變化 61 參考文獻 62 | |
| dc.language.iso | zh-TW | |
| dc.subject | 二十二碳六烯酸 | zh_TW |
| dc.subject | 糖皮質類固醇 | zh_TW |
| dc.subject | 下視丘 | zh_TW |
| dc.subject | 海馬 | zh_TW |
| dc.subject | 壓力 | zh_TW |
| dc.subject | hypothalamus | en |
| dc.subject | docosahexaenoic acid | en |
| dc.subject | glucocorticoid | en |
| dc.subject | hippocampus | en |
| dc.subject | stress | en |
| dc.title | 探討缺乏二十二碳六烯酸對雄性大鼠由壓力誘發糖皮質類固醇分泌之影響 | zh_TW |
| dc.title | Effects of docosahexaenoic acid deficiency on stress-induced glucocorticoid secretion in adult male rats | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-1 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 李立仁,呂紹俊,黃青真 | |
| dc.subject.keyword | 二十二碳六烯酸,糖皮質類固醇,下視丘,海馬,壓力, | zh_TW |
| dc.subject.keyword | docosahexaenoic acid,glucocorticoid,hypothalamus,hippocampus,stress, | en |
| dc.relation.page | 69 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-01-21 | |
| dc.contributor.author-college | 醫學院 | zh_TW |
| dc.contributor.author-dept | 生理學研究所 | zh_TW |
| 顯示於系所單位： | 生理學科所 | |
文件中的檔案：
| 檔案 | 大小 | 格式 | |
|---|---|---|---|
| ntu-102-1.pdf 未授權公開取用 | 2.31 MB | Adobe PDF |
系統中的文件，除了特別指名其著作權條款之外，均受到著作權保護，並且保留所有的權利。