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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 蘇慧敏 | |
dc.contributor.author | Shuo-Chueh Cheng | en |
dc.contributor.author | 鄭碩珏 | zh_TW |
dc.date.accessioned | 2021-06-16T16:24:54Z | - |
dc.date.available | 2018-03-04 | |
dc.date.copyright | 2013-03-04 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-01-22 | |
dc.identifier.citation | Asada H, Uyemura K, Shirao T (1994) Actin-Binding protein, drebrin, accumulates in submembranous regions in parallel with neuronal differentiation. Journal of Neuroscience Research 38:149-159.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/63144 | - |
dc.description.abstract | 二十二碳六烯酸(Docosahexaenoic acid, 22: 6 n-3, DHA)為多元n-3不飽和脂肪酸,主要富含於神經細胞膜,可促進神經細胞的分化與生長。DHA主要在大腦發育時期堆積,在大鼠中約出生前五天至出生後21天快速累積在腦中,故本實驗探討大鼠在大腦發育時期,腦部DHA及Growth-associated protein 43 (GAP-43)、Developmentally regulated brain proteins (Drebrin)、Brain-derived neurotrophic factor (BDNF)三種神經蛋白是否隨著大腦發育而改變,以及DHA缺乏,是否會影響此三種神經蛋白之表現。利用母鼠懷孕泌乳期餵予corn oil based之n-3缺乏飼料及n-3缺乏飼料加上每日補充攝取飼料熱量0.4%的n-3脂肪酸為n-3適量飼料當作控制組,取其子代出生第0、7、14、21天大幼鼠之brain stem、cerebellum、frontal cortex、hippocampus、hypothalamus、olfactory bulb及visual cortex等七個腦區利用氣相層析儀(gas chormatography)之火焰離子化偵測器(flame ionization detector )分析其脂肪酸之組成以及利用西方墨點法(Western blot)分析GAP-43、Drebrin及BDNF三種蛋白質表現。實驗結果發現DHA在大腦發育時期,七個腦區中DHA含量為隨著出生天數增加而上升,且n-3缺乏組DHA含量皆顯著低於控制組(DHA, n-3 deficient group: 5-11% of total fatty acid;control group:10-16% of total fatty acid )。而GAP-43、Drebrin及BDNF三種神經蛋白在大鼠七個腦區中的表現量,在整個大腦發育時期也是隨出生天數增加而改變,GAP-43蛋白為出生後持續上升至14-21天上升速度漸緩,而Drebrin則為出生後持續下降,BDNF為出生後持續上升至21天大。且此三種神經蛋白在n-3缺乏組中皆較低於控制組,尤其是在剛出生0-7天大時分析的七個腦區中的GAP-43、Drebrin及BDNF蛋白質表現在n-3缺乏組中皆顯著低於控制組。推測在大鼠大腦發育時期腦中缺乏DHA,會減少GAP-43、Drebrin及BDNF神經蛋白之表現,尤其是在大腦發育初期。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:24:54Z (GMT). No. of bitstreams: 1 ntu-102-R98441002-1.pdf: 3655822 bytes, checksum: 5bdfd74eb19ca2f389e488cd81fab9c5 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 目錄
中文摘要 I Abstract II 表目錄 V 圖目錄 VII 第壹章、文獻回顧 1 一、必需脂肪酸代謝途徑 1 二、DHA對於神經系統之重要性 2 三、GAP-43(Growth-associated protein 43) 3 四、Drebrin (Developmentally regulated brain proteins) 4 五、BDNF(Brain-derived neurotrophic factor) 5 第貳章、研究目標 7 第參章、材料與方法 8 一、實驗飼料 8 二、實驗動物與實驗設計 8 三、脂肪酸萃取及甲基化 9 四、氣相層析儀分析(gas chromatography, 7820 系統, Agilent Technologies): 10 五、蛋白質萃取 10 六、西方墨點法 11 1. 膠片製備 11 2. 樣本製備: 12 3. 電泳 13 4. 轉印: 13 5. 酵素免疫染色法: 14 6. 冷光呈色照相與定量: 15 七、統計方法 15 第肆章、實驗結果 16 一、大腦發育時期腦中脂肪酸之分析 16 二、DHA缺乏對於發育中大鼠腦部神經蛋白質表現之影響 17 1. GAP-43 (Growth-associated protein 43) 18 2. Drebrin (Developmentally regulated brain proteins) 18 3. BDNF (Brain-derived neurotrophic factor) 19 4. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) 20 第伍章、 討論 21 一、實驗動物模式之建立 21 二、大腦發育時期腦中DHA(C22: 6 n-3)、DPA(22:5 n-6)及AA(20:4 n-6)的變化 22 三、DHA缺乏對於發育中大鼠腦部神經蛋白表現的影響 23 1. GAP-43 (Growth-associated protein 43) 23 2. Drebrin (Developmentally regulated brain proteins) 25 3. BDNF (Brain-derived neurotrophic factor) 26 4. GAPDH (Glyceraldehyde-3-phosphate dehydrogenase) 27 第陸章、總結 28 第柒章、實驗圖表 29 第捌章、參考文獻 50 | |
dc.language.iso | zh-TW | |
dc.title | 探討二十二碳六烯酸缺乏對發育大鼠腦區神經蛋白質表現的影響 | zh_TW |
dc.title | Effects of docosahexaenoic acid deficiency on neuronal protein expression in the developing rat | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 黃青真,呂紹俊,李立仁 | |
dc.subject.keyword | 二十二碳六烯酸,GAP-43,Drebrin,BDNF,n-3不飽和脂肪酸, | zh_TW |
dc.subject.keyword | DHA,GAP-43,Drebrin,BDNF,n-3 polyunsaturated fatty acid, | en |
dc.relation.page | 53 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-01-23 | |
dc.contributor.author-college | 醫學院 | zh_TW |
dc.contributor.author-dept | 生理學研究所 | zh_TW |
顯示於系所單位: | 生理學科所 |
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