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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 黃鵬鵬 | |
dc.contributor.author | Chao-Yew Yang | en |
dc.contributor.author | 楊超宇 | zh_TW |
dc.date.accessioned | 2021-06-16T16:15:38Z | - |
dc.date.available | 2016-02-21 | |
dc.date.copyright | 2013-02-21 | |
dc.date.issued | 2012 | |
dc.date.submitted | 2013-02-06 | |
dc.identifier.citation | 1. Ranhotra HS 2010 The estrogen-related receptor alpha: the oldest, yet an energetic orphan with robust biological functions. J Recept Signal Transduct Res 30:193-205
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Bertrand S, Thisse B, Tavares R, Sachs L, Chaumot A, Bardet PL, Escriva H, Duffraisse M, Marchand O, Safi R 2007 Unexpected novel relational links uncovered by extensive developmental profiling of nuclear receptor expression. PLoS Genet 3:e188 41. Chang WJ, Hwang PP 2011 Development of zebrafish epidermis. Birth Defects Res C Embryo Today 93:205-214 42. Evans DH, Piermarini PM, Choe KP 2005 The multifunctional fish gill: dominant site of gas exchange, osmoregulation, acid-base regulation, and excretion of nitrogenous waste. Physiol Rev 85:97-177 43. Bardet PL, Horard B, Laudet V, Vanacker JM 2005 The ERRα orphan nuclear receptor controls morphogenetic movements during zebrafish gastrulation. Dev Biol 281:102-111 44. Yan JJ, Chou MY, Kaneko T, Hwang PP 2007 Gene expression of Na+/H+ exchanger in zebrafish H+-ATPase-rich cells during acclimation to low-Na+ and acidic environments. Am J Physiol Cell Physiol 293:C1814-C1823 45. Michalek RD, Gerriets VA, Jacobs SR, Macintyre AN, MacIver NJ, Mason EF, Sullivan SA, Nichols AG, Rathmell JC 2011 Cutting edge: distinct glycolytic and lipid oxidative metabolic programs are essential for effector and regulatory CD4+ T cell subsets. J Immunol 186:3299-3303 46. Shi LZ, Wang R, Huang G, Vogel P, Neale G, Green DR, Chi H 2011 HIF1alpha-dependent glycolytic pathway orchestrates a metabolic checkpoint for the differentiation of TH17 and Treg cells. J Exp Med 208:1367-1376 47. Schreiber SN, Knutti D, Brogli K, Uhlmann T, Kralli A 2003 The transcriptional coactivator PGC-1 regulates the expression and activity of the orphan nuclear receptor estrogen-related receptor α (ERRα). J Biol Chem 278:9013-9018 48. Puigserver P, Spiegelman BM 2003 Peroxisome proliferator-activated receptor-γ coactivator 1α (PGC-1α): transcriptional coactivator and metabolic regulator. Endocr Rev 24:78-90 49. Scarpulla RC 2011 Metabolic control of mitochondrial biogenesis through the PGC-1 family regulatory network. Biochim Biophys Acta 1813:1269-1278 50. Liu Y, Chen Q, Gupta N, Fang F, Chang Y 2010 PGC-1b-Regulated mitochondrial biogenesis and function in myotubes is mediated by NRF-1 and ERRa. Mitochondrion 10:516-527 51. Mirebeau-Prunier D, Le Pennec S, Jacques C, Gueguen N, Poirier J, Malthiery Y, Savagner F 2010 Estrogen-related receptor alpha and PGC-1-related coactivator constitute a novel complex mediating the biogenesis of functional mitochondria. FEBS J 277:713-725 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62937 | - |
dc.description.abstract | 相較於陸生哺乳動物而言,魚類必須面對更多滲透壓及離子平衡調控上的挑戰,因此專職於離子運輸的離子細胞尤其得耗費更多的能量去掌控離子運輸的生理功能以面對不同滲透壓環境下對魚體造成的壓力。過去研究類雌激素受體(ERR)的主要功能多為調控能量上的代謝及粒線體之生理功能,而調控離子平衡的角色或其所需的能量運作上鮮少研究提及。離子細胞為一種高能量需求且富含粒線體的細胞,在魚類的滲透壓及離子平衡調控上扮演重要角色。本實驗目的研究ERR在斑馬魚離子運輸及滲透壓平衡中所扮演的角色及調控機制。過去的研究指出ERR中僅兩型受體α及δ之mRNA呈現胡椒鹽狀分布於斑馬魚胚胎表皮上,這種分布情形極為類似離子細胞散布狀態,故本研究針對此兩型受體作了以下實驗,實驗中發現在酸環境下會刺激ERRα及δ表現上升,且此兩型mRNA皆表現於富氫幫浦細胞(H+-ATPase-rich cell, HRC),但在分別抑制此兩型受體蛋白表現後,僅α型會影響氫離子運輸功能,而δ型則否;另一影響HRC分化之轉錄因子膠細胞迷失因子2 (glial cell missing 2)表現量上升試圖彌補因ERRα抑制所造成的HRC數量的下滑。綜合以上所述,ERRα經由調控斑馬魚胚胎時期HRC分化參與了氫離子運輸之功能,而ERRδ在HRC中所參與的功能仍待研究。 | zh_TW |
dc.description.abstract | Compared to terrestrial mammals, fish have to cope with more challenging osmotic and ionic gradients from aquatic environment with variant ion composition and pH values. Ionocytes, are highly energy-demanding and studied cells in research on fish ionoregulation, contribute an excellent model to study the regulatory mechanisms of ion transport. Estrogen-related receptors (ERRs) are members of orphan nuclear receptors and ubiquitously express in high-energy demanding organs for energy metabolism. Recently, ERRα was especially studied in mammalian kidney for sodium and potassium homeostasis through renin-angiotensin aldosterone system. In fact, skin/gill ionocytes in zebrafish are analogous to several types of the ion-transporting cells in mammalian kidney in terms of the expression and function of ion transporters. The purpose of the present study is to explore the role of zebrafish ERRs in ion regulatory mechanism by using molecular physiological approaches. zERRα and δ mRNA distributions showed “salt-and-pepper” patterns similar to ionocytes expression on the skin of zebrafish embryos so they would be the targets of the present study. The zERRα and δ mRNA expression were found to be stimulated by low pH ambience and also localized in HRC. However, in the loss-of-function experiments, the impairment of proton secretion only occurred when zERRα, but not zERRδ. Further, knockdown of zERRα decreased the cell density of H+-ATPase-rich cell (HRC) even with a concomitant increase of zebrafish glial cell missing 2 (zgcm2; a transcription factor specific for HRC differentiation) expression in zebreafish embryos. Hence, zERRα may play control zebrafish acid secretion function through regulation of zGCM2-mediated cell differentiation of HRC in the skin/gill. On the other hand, it will be an interesting and challenging issue to see if zERRδ, the other zERR member expressing in HRC, play an indirect role in acid secretion or is involved in other functions of zebrafish HRC. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:15:38Z (GMT). No. of bitstreams: 1 ntu-101-R99b45022-1.pdf: 2527748 bytes, checksum: ac63277b4d45a4001963b791aea1c611 (MD5) Previous issue date: 2012 | en |
dc.description.tableofcontents | 口試委員審定書 i
誌謝 ii 中文摘要 1 Abstract 2 Introduction 4 Estrogen-related receptors 4 The role of estrogen-related receptors in energy metabolism 5 The role of estrogen-related receptors in cell differentiation 6 The role of estrogen-related receptors in ion homeostasis 7 The role of estrogen-related receptors in fish 9 Zebrafish as model to study fish in iono- and osmo-regulatory mechanisms 10 Purpose 11 Material and methods 12 Experimental fish 12 Acclimation to different artificial freshwater containing different Na+ /Cl- levels 12 Acclimation to different pH environments 13 Total RNA isolation 14 Reverse-transcription polymerase chain reaction (RT-PCR) analysis 14 Real-time quantitative PCR analysis 15 Whole-mount in situ hybridization 16 Immunocytochemistry 18 Microinjection of antisense morphlino oligonucleotides 19 Westernblot and sequence aliment analysis 20 H+-selective electrode technique 20 Surface pH of zebrafish embryos 21 Statistical analysis 22 Results 23 Effects of environmental ion concentration and pH on mRNA expression of zesrra, zesrrd, and zpgc1a in zebrafish gill and embryos 23 Localizations of zesrra, zesrrd and zpgc1a mRNA in zebrafish ionocytes 24 Effects of MO knockdown on protein expression and morphology of zebrafish larvae 25 The effects of zERRα and zERRδ knockdown on proton secretion of zebrafish embryos 26 The effects of zERRα knockdown on HRC-related gene expression 26 The effects of zERRα knockdown on ionocytes differentiation/proliferation 27 Discussion 29 Acidic environment induces gene expressions of zERRα, zERRδ, and zPGC1α 29 zERRα participates in acid secretion function in HRC 31 ERRα participates in acid secretion mechanism via regulation of zGCM2-mediated HRC differentiation 33 zPGC1α is not involved in the zERRα-mediated acid secretory mechanism 35 Conclusions and perspectives 36 Reference 38 Tables and figures 43 | |
dc.language.iso | en | |
dc.title | 類雌激素受體在斑馬魚離子細胞分化及功能調控之角色 | zh_TW |
dc.title | The Role of Estrogen-Related Receptor in Ionocyte Differentiation and Ionoregulation of Zebrafish (Danio rerio) | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張清風,林豊益,曾庸哲 | |
dc.subject.keyword | 類雌激素受體,離子調控,酸鹼平衡,離子細胞,斑馬魚, | zh_TW |
dc.subject.keyword | estrogen-related receptor,ionoregulation,acid-base homeostasis,ionocyte,zebrafish, | en |
dc.relation.page | 69 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-02-06 | |
dc.contributor.author-college | 生命科學院 | zh_TW |
dc.contributor.author-dept | 漁業科學研究所 | zh_TW |
顯示於系所單位: | 漁業科學研究所 |
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