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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 朱士維(Shi-Wei Chu) | |
dc.contributor.author | Chih-Yuan Yang | en |
dc.contributor.author | 楊治原 | zh_TW |
dc.date.accessioned | 2021-06-16T16:15:24Z | - |
dc.date.available | 2016-02-21 | |
dc.date.copyright | 2013-02-21 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-02-06 | |
dc.identifier.citation | [1] J. C. Misra, G. C. Shit, and H. J. Rath, 'Flow and heat transfer of a MHD viscoelastic fluid in a channel with stretching walls: Some applications to haemodynamics,' Computers & Fluids, vol. 37, pp. 1-11, Jan 2008.
[2] K. Dowlatshahi, D. Babich, J. D. Bangert, and R. Kluiber, 'Histologic evaluation of rat mammary-tumor necrosis by interstitial nd-yag laser hyperthermia,' Lasers in Surgery and Medicine, vol. 12, pp. 159-164, 1992 1992. [3] J. C. Bischof, 'Micro and nanoscale phenomenon in bioheat transfer,' Heat and Mass Transfer, vol. 42, pp. 955-966, Aug 2006. [4] J. D. Hazle, C. J. Diederich, M. Kangasniemi, R. E. Price, L. E. Olsson, and R. J. Stafford, 'MRI-guided thermal therapy of transplanted tumors in the canine prostate using a directional transurethral ultrasound applicator,' Journal of Magnetic Resonance Imaging, vol. 15, pp. 409-417, Apr 2002. [5] S. T. Cegg and R. B. Roemer, 'Towards the estimation of three-dimensional temperature fields from noisy temperature measurements during hyperthermia,' International Journal of Hyperthermia, vol. 5, pp. 467-484, 1989 1989. [6] W. Casscells, B. Hathorn, M. David, T. Krabach, W. K. Vaughn, H. A. McAllister, et al., 'Thermal detection of cellular infiltrates in living atherosclerotic plaques: possible implications for plaque rupture and thrombosis,' Lancet, vol. 347, pp. 1447-51, May 25 1996. [7] B. Quesson, J. A. de Zwart, and C. T. W. Moonen, 'Magnetic resonance temperature imaging for guidance of thermotherapy,' Journal of Magnetic Resonance Imaging, vol. 12, pp. 525-533, Oct 2000. [8] I. A. Vitkin, J. A. Moriarty, R. D. Peters, M. C. Kolios, A. S. Gladman, J. C. Chen, et al., 'Magnetic resonance imaging of temperature changes during interstitial microwave heating: A phantom study,' Medical Physics, vol. 24, pp. 269-277, Feb 1997. [9] J. M. Vroom, K. J. De Grauw, H. C. Gerritsen, D. J. Bradshaw, P. D. Marsh, G. K. Watson, et al., 'Depth penetration and detection of pH gradients in biofilms by two-photon excitation microscopy,' Applied and Environmental Microbiology, vol. 65, pp. 3502-3511, Aug 1999. [10] I. Georgakoudi, B. C. Jacobson, M. G. Muller, E. E. Sheets, K. Badizadegan, D. L. Carr-Locke, et al., 'NAD(P)H and collagen as in vivo quantitative fluorescent biomarkers of epithelial precancerous changes,' Cancer Research, vol. 62, pp. 682-687, Feb 1 2002. [11] K. Schenke-Layland, I. Riemann, O. Damour, U. A. Stock, and K. Konig, 'Two-photon microscopes and in vivo multiphoton tomographs--powerful diagnostic tools for tissue engineering and drug delivery,' Adv Drug Deliv Rev, vol. 58, pp. 878-96, Sep 15 2006. [12] W. K. Li and P. M. Dickie, 'Monitoring phytoplankton, bacterioplankton, and virioplankton in a coastal inlet (Bedford Basin) by flow cytometry,' Cytometry, vol. 44, pp. 236-46, Jul 1 2001. [13] U. Schreiber, K. Colbow, and W. Vidaver, 'Analysis of temperature-jump chlorophyll fluorescence induction in plants,' Biochimica Et Biophysica Acta, vol. 423, pp. 249-263, 1976 1976. [14] C.-S. Liao, 'Master thesis:Observation of heat propagation and heat induced denaturation in biological tissues by non-linear optical microscopy,' 2010. [15] W. Denk, J. H. Strickler, and W. W. Webb, '2-Photon Laser Scanning Fluorescence Microscopy,' Science, vol. 248, pp. 73-76, Apr 6 1990. [16] M. Oheim, D. J. Michael, M. Geisbauer, D. Madsen, and R. H. Chow, 'Principles of two-photon excitation fluorescence microscopy and other nonlinear imaging approaches,' Advanced Drug Delivery Reviews, vol. 58, pp. 788-808, Sep 15 2006. [17] K. Koenig, 'Multiphoton microscopy in life sciences,' Journal of Microscopy (Oxford), vol. 200, pp. 83-104, November 2000. [18] W.-L. Chang, 'Master thesis:Tunable pulse laser generation from 1.3μm to 1.8μm by periodically poled lithium niobate,' 1921. [19] J. P. Thornber, R. P. F. Gregory, C. A. Smith, and J. L. Bailey, 'Studies on nature of chloroplast lamella .I. Preparation and some properties of 2 chlorophyll-protein complexes,' Biochemistry, vol. 6, pp. 391-&, 1967 1967. [20] U. Niinemets, 'Components of leaf dry mass per area - thickness and density - alter leaf photosynthetic capacity in reverse directions in woody plants,' New Phytologist, vol. 144, pp. 35-47, Oct 1999. [21] R. L. Hays, 'Thermal-conductivity of leaves,' Planta, vol. 125, pp. 281-287, 1975 1975. [22] H. Hedlund and P. Johansson, 'Heat capacity of birch determined by calorimetry: implications for the state of water in plants,' Thermochimica Acta, vol. 349, pp. 79-88, Apr 1 2000. [23] E. T. Linacre, 'Determinations of heat transfer coefficient of leaf,' Plant Physiology, vol. 39, pp. 687-&, 1964 1964. [24] P. Joliot and A. Joliot, 'Different types of quenching involved in photosystem II centers,' Biochim Biophys Acta, vol. 305, pp. 202-16, May 30 1973. [25] I. Vass, S. Styring, T. Hundal, A. Koivuniemi, E. Aro, and B. Andersson, 'Reversible and irreversible intermediates during photoinhibition of photosystem II: stable reduced QA species promote chlorophyll triplet formation,' Proc Natl Acad Sci U S A, vol. 89, pp. 1408-12, Feb 15 1992. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62931 | - |
dc.description.abstract | 熱在生醫領域的應用受到越來越廣泛的重視。舉例而言:對病患受傷部位附近的局部組織加熱以加速新陳代謝,可使傷口恢復的方式;或是透過小區域以高溫加熱方式殺死體內病變組織或癌細胞,替代傳統放射性治療的療程;此外,相較於透過高溫殺死細胞,冷凍手術則是以極低的溫度讓欲切除的細胞組織壞死,這樣的好處是壞死脫落處的傷口較小,可以減少出血情形以及降低病人的疼痛。以上種種不論是利用高溫或低溫,都是屬於熱療法的範疇;而它們的機制均是針對患者身體一小部份的組織,造成不均勻的溫度梯度分佈,以達到醫師或治療師所希望達成的某些目的。針對熱療法的最佳化以及減少非必要的組織傷害,需要比較好的溫度影像。最直接獲得熱影像的方式便是插入熱電偶來獲得接觸部位的溫度,但這種方法具有侵入性,而且空間上的解析度受限於探針尖端的尺寸。紅外線熱影像是另一種發展許久的技術,亦有其限制:除了因為繞射極限造成空間解析度受限之外,只能得到表面的溫度影像資訊是最大的致命傷。
承上,我們提出了問題:「是否可以找到某種溫度相關的物理量變化機制,可以在呈現熱影像時,具備高空間解析度,以及非侵入式探測的性質?」 由於螢光分子受到熱影響會改變其發光強度,換句話說螢光強度對溫度有相依性;而另一方面,在生物組織內有許多分子具有自發螢光的特性,例如:膠原蛋白、NAD(P)H、黑色素、葉綠素…等等。結合螢光本身對溫度有關聯的特性,以及生物組織內含有數種自發螢光分子這兩件事實,暗示了只要釐清螢光強度和溫度之間的關係,我們將可以做到利用光學方法觀察熱在生物組織內的流動。由於葉綠素分子的激發波長最符合本實驗室已經具備的光源,同時葉子也是相對容易取得的樣品,因此選用葉子作為本實驗的樣本。 過去,有研究指出,葉子所產生的自發螢光強度會隨著溫度的改變而變化,藉由量測自發螢光的強度,可以推論溫度的高低。啟發自此研究,本篇論文的主要目的為利用雙光子螢光顯微術,以光學非侵入式的方法,於葉片內取得具有細胞解析度等級的三維立體螢光強度影像。並藉由建立螢光強度與溫度轉換模型,將螢光強度的空間分佈影像,轉換為溫度梯度分佈影像。透過此溫度梯度的變化分析,可以進一步提供估計組織內熱致相變化的能力。我們的方法提供的空間解析度為1微米,溫度解析度為0.6oC的非侵入式三維立體熱影像,是當前臨床所採用的熱影像方法所無法企及的。激發螢光的方式採用雙光子螢光激發,最主要的原因在於這樣非線性的激發方式提供了細胞等級的三維成像可能,這是目前的熱影像方法所無法企及的。另外透過螢光變化,還提供估計熱致相變化的能力。總結以上,本篇論文主要的方向在於建立一套模型,藉由分析葉子螢光強度的改變,可以觀察熱在組織內的傳播狀況,尺度上可以達到優於細胞等級的空間解析度,0.6oC的溫度解析度,並且具備三維的成像能力。這個方法可以提供非侵入式以及高解析度的探測,對於釐清和熱相關的生物物理機制將會有許多助益。 | zh_TW |
dc.description.abstract | The applications of heat play more and more important roles in biomedical field. For example, applying heat to injured parts will be able to speed up the metabolism, and accelerate recovery. High temperature can kill malignant cell instead of radiation treatment. Cryosurgical procedure is a technique which employs the use of cooling to destroy cells, with the advantage of less bleeding and reducing pain. All of the above belong to field of thermal therapies. To optimize thermal therapy and reduce thermal damage, we need better thermal imaging. A straightforward way to measure the temperature is thermal couple which is invasive and is limited to single-point detection. Infrared thermography is a convenient way to observe thermal imaging but limited to surface image.
Hence, we would like to know that “ Are there temperature-related mechanism to present thermal imaging with the capability of high spatial resolution and non-invasiveness ? ” There exist some correlation between fluorescence intensity and temperature. In addition, there are many molecules inside bio-tissue emitting auto-fluorescence, such as collagen, NAD(P)H, melanin, chlorophyll. Following these two facts, it thus comes to us that when we know the relationship between fluorescence intensity and temperature inside bio-tissue, we are able to observe heat propagation in bio-tissues by optical method. Our lab have already equipped with the light source matched the excitation wavelength of chlorophyll and also leaves are much easier to acquire, hence we finally choose leaf as our experimental sample. According to literature, it is known that auto-fluorescence intensity of leaf is strongly dependent on local temperature, we use leaf as our sample to visualize thermal imaging. In this study, we demonstrate by using time-lapsed two-photon fluorescence microscopy (2PFM), heat flow in biological tissues can be monitored based on temperature-induced fluorescence change of endogenous molecules. Compared with conventional infrared thermography or other thermal imaging tools, 2PFM provides both sub-micrometer spatial resolution and optical sectioning capability inside tissues. From the analysis of temporal variation in fluorescence, not only detailed temperature distribution can be quantified, but also the energy absorption in bio-tissue could be estimated. In this research, we have achieved the prospect of observing heat propagation in bio-tissues with spatial resolution better than cellular scale, 0.6oC thermal resolution and 3-dimensinal capability! Such a technique will be useful for the characterization of heat-related biophysical mechanisms with high spatial resolution in thick tissues non-invasively. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:15:24Z (GMT). No. of bitstreams: 1 ntu-102-R98245011-1.pdf: 5935324 bytes, checksum: a8a8d7cfc1b62e6b62ce47b180dc153d (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 口試委員會審定書 #
誌謝 i 中文摘要 ii ABSTRACT iv CONTENTS vi LIST OF FIGURES viii LIST OF TABLES ix 第一章 導論................................................1 1-1 熱在生醫領域的應用........................................1 1-2 傳統熱影像方法...........................................2 1-3 其他熱影像方法...........................................3 1-4 本篇論文研究方向..........................................4 第二章 基本原理.............................................5 2-1 螢光:單光子螢光與雙光子螢光顯微技術.........................5 2-2 螢光和熱的關係...........................................9 第三章 樣本選取與實驗架設....................................11 3-1 實驗系統架設:雙光子螢光顯微系統以及加熱器材..................11 3-2 進行加熱前的測試........................................14 3-3 樣本準備以及實驗步驟.....................................16 3-4 利用螢光影像觀察”熱流動”................................17 第四章 結果分析............................................19 4-1 溫度變化改變的葉綠素螢光特性...............................19 4-2 決定區域溫度............................................23 4-3 溫度解析度.............................................24 4-4 推導相變化所吸收的能量....................................25 第五章 結論...............................................32 附錄......................................................33 參考文獻...................................................39 | |
dc.language.iso | zh-TW | |
dc.title | 雙光子螢光顯微技術觀察生物組織內熱傳播現象 | zh_TW |
dc.title | Visualization of heat propagation in biological tissues by two photon fluorescence microscopy | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 董成淵(Chen-Yuan Dong),詹明哲(Ming-Che Chan),林彥穎(Yen-Yin Lin) | |
dc.subject.keyword | 雙光子螢光顯微術,葉綠素,溫度,熱傳播, | zh_TW |
dc.subject.keyword | two photon fluorescence microscopy,chlorophyll,temperature,heat propagation, | en |
dc.relation.page | 40 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-02-06 | |
dc.contributor.author-college | 理學院 | zh_TW |
dc.contributor.author-dept | 應用物理所 | zh_TW |
顯示於系所單位: | 應用物理研究所 |
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