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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張孟基(Men-Chi Chang) | |
dc.contributor.author | Ya-Chen Huang | en |
dc.contributor.author | 黃雅貞 | zh_TW |
dc.date.accessioned | 2021-06-16T16:13:58Z | - |
dc.date.available | 2023-02-07 | |
dc.date.copyright | 2013-03-06 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-02-07 | |
dc.identifier.citation | 戶刈義次 (1963) 作物學試驗法 東京農業技術學會印行 第159-176頁
Aroca R, Porcel R, Ruiz-Lozano JM. (2012) Regulation of root water uptake under abiotic stress conditions. J Exp. Bot. 63:43-57 Bach L, Faure JD. (2010) Role of very-long-chain fatty acids in plant development, when chain length does matter. C. R. Biol. 333:361-370 Beaudoin F, Wu X, Li F, Haslam RP, Markham JE, Zheng H, Napier JA, Kunst L. (2009) Functional characterization of the Arabidopsis beta-ketoacyl-coenzyme A reductase candidates of the fatty acid elongase. Plant Physiol. 150:1174-1191 Brady SM, Orlando DA, Lee JY, Wang JY, Koch J, Dinneny JR, Mace D, Ohler U, Benfey PN. (2007) A high-resolution root spatiotemporal map reveals dominant expression patterns. Science 318:801-806 Brown DE, Rashotte AM, Murphy AS, Tague BW, Peer WA, Taiz L and Mudat GK. (2001) Flavonoids act as negative regulators of auxin transport in vivo in Arabidopsis thaliana. Plant Physiol. 126: 524-535 Buer CS, Muday GK, Djordjevic MA. (2007) Flavonoids are differentially taken up and transported long distances in Arabidopsis Plant Physiol 145:478-490 Brundrett MC, Kendrick B and Peterson CA. (1991) Efficient lipid staining in plant material with sudan red 7B or fluoral yellow 088 in polyethylene glycol-glycerol. Biotech. Histochem. 66:111-116 Chae K, Kieslich CA, Morikis D, Kim SC, Lord EM. (2009) A gain-of-function mutation of Arabidopsis lipid transfer protein 5 disturbs pollen tube tip growth and fertilization. Plant Cell. 21:3902-3914 Chae K, Gonong BJ, Kim SC, Kieslich CA, Morikis D, Balasubramanian S, Lord EM. (2010) A multifaceted study of stigma/style cysteine-rich adhesin (SCA)-like Arabidopsis lipid transfer proteins (LTPs) suggests diversified roles for these LTPs in plant growth and reproduction. J Exp Bot. 61:4277-4290 Chen CW, Yang YW, Lur HS, Tsai YG, Chang MC. (2006) A novel function of abscisic acid in the regulation of rice (Oryza sativa L.) root growth and development. Plant Cell Physiol. 47:1-13 Carol RJ, Dolan L. (2006) The role of reactive oxygen species in cell growth: lessons from root hairs. J Exp Bot. 57:1829-1834 Cohen D, Bogeat-Triboulot M, Tisserant E, Balzergue S, Martin-Magniette M, Lelandais G, Ningre N, Renou J, Tamby j, Thiec D, Hummel I. (2010) Comparative transcriptomics of drought responses in Populus: a meta-analysis of genome-wide expression profiling in mature leaves and root apices across two genotypes. BMC Genomics 11:630-640 Cotsaftis O, Plett D, Johnson AA, Walia H, Wilson C, Ismail AM, Close TJ, Tester M, Baumann U. (2011) Root-specific transcript profiling of contrasting rice genotypes in response to salinity stress. Mol. Plant. 4:25-41 De Smet I, Signora L, Beeckman T, Inzé D, Foyer CH, Zhang H. (2003) An abscisic acid-sensitive checkpoint in lateral root development of Arabidopsis. Plant J. 33:543-555 Deak KI, Malamy J. (2005) Osmotic regulation of root system architecture. Plant J. 43:17-28 Dinneny JR, Long TA, Wang JY, Jung JW, Mace D, Pointer S, Barron C, Brady SM, Schiefelbein J, Benfey PN. (2008) Cell identity mediates the response of Arabidopsis roots to abiotic stress. Science 320:942-945 Fujita M, Fujita Y, Noutoshi Y, Takahashi F, Narusaka Y, Yamaguchi-Shinozaki K, Shinozaki K. (2006) Crosstalk between abiotic and biotic stress responses: a current view from the points of convergence in the stress signaling networks. Curr. Opin. Plant Biol. 9:436-442 Gonzalez-Carranza ZH, Elliott KA, Roberts JA. (2007) Expression of polygalacturonases and evidence to support their role during cell separation processes in arabidopsis thaliana. J. Exp. Bot. 58:3719-3730 Hirano K, Aya K, Hobo T, Ueguchi-Tanaka M, Sakakibara H, Kojima M and Matsuoka M. (2008) Comprehensive transcriptome analysis of phytohormone biosynthesis and signaling genes in microspore/pollen and tapetum of rice. Plant Cell Physiol. 49: 1429-1450 Jacobs M. and Rubery P.H. (1988) Naturally occurring auxin transport regulators. Science 241: 346-349 Jouhet J, Marechal E, Block MA. (2007) Glycerolipid transfer for the building of membranes in plant cells. Prog. Lipid Res. 46:37-55 Jiao Y, Tausta SL, Gandotra N, Sun N, Liu T, Clay NK, Ceserani T, Chen M., Ma L, Holford M, Zhang HY, Zhao H, Deng XW, Nelson T. (2009) A transcriptome atlas of rice cell types uncovers cellular, functional and developmental hierarchies. Nat. Genet. 41:258-263 Kader JC. (1997) Lipid-transfer proteins: a puzzling family of plant proteins. Plant Science 2:66-70 Kobayashi Y, Yamamoto S, Minami H, Kagaya Y, Hattori T. (2004) Differential activation of the rice sucrose nonfermenting1-related protein kinase2 family by hyperosmotic stress and abscisic acid. Plant Cell 16:1163-1177 Kyndt T, Denil S, Haegeman A, Trooskens G, De Meyer T, Van Criekinge W, Gheysen G. (2012) Transcriptome analysis of rice mature root tissue and root tips in early development by massive parallel sequencing. J. Exp. Bot. 63:2141-2157 Lenka SK, Katiyar A, Chinnusamy V, Bansal KC. (2010) Comparative analysis of drought-responsive transcriptome in Indica rice genotypes with contrasting drought tolerance. Plant Biotechnol. J. 9:315-327 Lewis DR, Ramirez MV, Miller ND, Vallabhaneni P, Ray WK, Helm RF, Winkel BS,Muday GK. (2011) Auxin and ethylene induce flavonol accumulation through distinct transcriptional networks. Plant Physiol. 156:144-164 Lorenz WW, Alba R, Yu Y-S, Bordeaux JM, Simoes M, Dean Jeffrey FD (2011) Microarray analysis and scale-free gene networks identify candidate regulators in drought-stressed roots of loblolly pine (P. taeda L.). BMC Genomics 12:264 Lin M, Kilaru A, and Hasenstein KH. (2008) Abscisic acid response of corn (Zea mays L.) roots and protoplasts to lanthanum. J. Plant Growth Regul. 27:19-25 Lynch J. (1995) Root architecture and plant productivity. Plant Physiol. 109:7–13 Malamy JE. (2005) Intrinsic and environmental response pathways that regulate root system architecture. Plant Cell Environ. 28:67–77 Miller G, Suzuki N, Ciftci-Yilmaz S, Mittler R. (2010) Reactive oxygen species homeostasis and signalling during drought and salinity stresses. Plant Cell Environ. 33:453-467 Murphy A, Peer WA, and Taiz L. (2000). Regulation of auxin transport by aminopeptidases and endogenous flavonoids. Planta 211:315-324 Nibau C, Gibbs DJ, Coates JC. (2008) Branching out in new directions: the control of root architecture by lateral root formation. New Phytol. 179:595-614 Nijhawan A, Jain M, Tyagi AK, Khurana JP. (2008) Genomic survey and gene expression analysis of the basic leucine zipper transcription factor family in rice. Plant Physiol. 146:333–350 Osmont KS, Sibout R, Hardtke CS. (2007) Hidden branches: developments in root system architecture. Annu. Rev. Plant Biol. 58:93-113 Potters G, Pasternak TP, Guisez Y, Jansen MA. (2009) Different stresses, similar morphogenic responses: integrating a plethora of pathways. Plant Cell Environ. 32:158-169 Potters G, Pasternak TP, Guisez Y, Palme KJ, Jansen MA. (2007) Stress-induced morphogenic responses: growing out of trouble? Trends Plant Sci. 12:98-105 Ray S, Dansana PK, Giri J, Deveshwar P, Arora R, Agarwal P, Khurana JP, Kapoor S, Tyagi AK. (2010) Modulation of transcription factor and metabolic pathway genes in response to water-deficit stress in rice. Funct Integr Genomics. 11:157-178 Rebouillat J, Dievart A, Verdeil JL, Escoute J, Giese G, Breitler JC, Gantet P, Espeout S, Guiderdoni E, Perin C. (2009) Molecular genetics of rice root development. Rice 2:15-34 Seo JS, Joo J, Kim MJ, Kim YK, Nahm BH, Song SI, Cheong JJ, Lee JS, Kim JK, Choi YD. (2011) OsbHLH148, a basic helix-loop-helix protein, interacts with OsJAZ proteins in a jasmonate signaling pathway leading to drought tolerance in rice. Plant J. 65:907-921 Signora L, Signora L, De Smet I, Foyer CH, Zhang H. (2001) ABA plays a central role in mediating the regulatory effects of nitrate on root branching in Arabidopsis. Plant J. 28:655–662 Takehisa H, Sato Y, Igarashi M, Abiko T, Antonio BA, Kamatsuki K, Minami H, Namiki N, Inukai Y, Nakazono M, Nagamura Y. (2012) Genome-wide transcriptome dissection of the rice root system: implications for developmental and physiological functions. Plant J. 69:126-140 Takeuchi K, Gyohda A, Tominaga M, Kawakatsu M, Hatakeyama A, Ishii N, Shimaya K, Nishimura T, Riemann M, Nick P, Hashimoto M, Komano T, Endo A, Okamoto T,Jikumaru Y, Kamiya Y, Terakawa T, Koshiba T. (2011) RSOsPR10 expression in response to environmental stresses is regulated antagonistically by jasmonate/ethylene and salicylic acid signaling pathways in rice roots. Plant Cell Physiol. 52:1686-1696 Teo YH, Beyrouty CA, Norman RJ and Gbur EE. (1995) Nutrient uptake relationship to root characteristics of rice. Plant Soil 171: 297–302 Walia H, Wilson C, Condamine P, Liu X, Ismail AM, Zeng L, Wanamaker SI, Mandai J, Cui X and Close TJ. (2005) Comparative transcriptional profiling of two contrasting rice genotypes under salinity stress during the vegetative growth stage. Plant Physiol. 139:822-835 Wang C, Yang C, Gao C, Wang Y. (2009) Cloning and expression analysis of 14 lipid transfer protein genes from Tamarix hispida responding to different abiotic stresses. Tree Physiol. 29:1607-1619 Winkel-Shirley B. (2002) Biosynthesis of flavonoids and effects of stress. Curr. Opin. Plant Biol. 5:218-223 Xue HW, Chen X, Mei Y. (2009) Function and regulation of phospholipid signalling in plants. Biochem. J. 421:145-156 Xu W, Jia L, Shi W, Liang J, Zhou F, Li Q, Zhang J. (2012) Abscisic acid accumulation modulates auxin transport in the root tip to enhance proton secretion for maintaining root growth under moderate water stress. New phytol. DOI: 10.1111/nph.12004 Xue T, Wang D, Zhang S, Ehlting J, Ni F, Jakab S, Zheng C, Zhong Y. (2008) Genome-wide and expression analysis of protein phosphatase 2C in rice and Arabidopsis. BMC Genomics 9:550 Yoshii M, Yamazaki M, Rakwal R, Kishi-Kaboshi M, Miyao A, Hirochika H. (2010) The NAC transcription factor RIM1 of rice is a new regulator of jasmonate signaling. Plant J. 61:804-815 Yu LJ, Luo YF, Liao B, Xie LJ, Chen L, Xiao S, Li JT, Hu SN, Shu WS. (2012) Comparative transcriptome analysis of transporters, phytohormone and lipid metabolism pathways in response to arsenic stress in rice (Oryza sativa). New Phytol. 195:97-112 Yun KY, Park MR, Mohanty B, Herath V, Xu F, Mauleon R, Wijaya E, Bajic VB, Bruskiewich R, de Los Reyes BG. (2010) Transcriptional regulatory network triggered by oxidative signals configures the early response mechanisms of japonica rice to chilling stress. BMC Plant Biol.10:16 Zhang D, Liang W, Yin C, Zong J, Gu F, Zhang D. (2010) OsC6, encoding a lipid transfer protein, is required for postmeiotic anther development in rice. Plant Physiol. 154:149-162 Zhang H, Han W, De Smet I, Talboys P, Loya R, Hassan A, Rong H, Jürgens G, Paul Knox J, Wang MH. (2010) ABA promotes quiescence of the quiescent centre and suppresses stem cell differentiation in the Arabidopsis primary root meristem. Plant J. 64:764-774 Zhu X, Li Z, Xu H, Zhou M, Du L, Zhang Z. (2012) Overexpression of wheat lipid transfer protein gene TaLTP5 increases resistances to Cochliobolus sativus and Fusarium graminearum in transgenic wheat. Funct Integr Genomics. 12:481-488 戶刈義次 (1963) 作物學試驗法 東京農業技術學會印行 第159-176頁 Abdrakhamanova A, Wang QY, Khokhlova L, Nick P (2003) Is microtubule disassembly a trigger for cold acclimation? Plant Cell Physiol 44:676–686 Abe H, Urao T, Ito T, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003) Arabidopsis AtMYC2 (bHLH) and AtMYB2 (MYB) function as transcriptional activators in abscisic acid signaling. Plant Cell 15:63–78 Alexandersson E, Danielson JAQ, Rade J, Moparthi VK, Fontes M, Kjellbom P, Johanson U (2010) Transcriptional regulation of aquaporins in accessions of Arabidopsis in response to drought stress. Plant J 61:650–660 Allwood EG, Anthony RG, Smertenko AP, Reichelt S, Drobak BK, Doonan JH, Weeds AG,Hussey PJ (2002) Regulation of the pollen-specific actin-depolymerizing factor LlADF1. Plant Cell 14:2915–2927 Ali GM, Komatsu S (2006) Proteomic analysis of rice leaf sheath during drought stress. J Proteome Res 5:396–403 Augustine RC, Vidali L, Kleinman KP, Bezanilla M (2008) Actin depolymerizing factor is essential for viability in plants, and its phosphoregulation is important for tip growth. Plant J 54:863–875 Basisakh N, Subudhi PK (2009) Heat stress alters the expression of salt stress induced genes in smooth cordgrass (Spartina alterniflora L.). Plant Physiol Biochem 47:232–235 Burgos-Rivera B, Ruzicka DR, Deal RB, McKinney EC, King-Reid L, Meagher RB (2008) ACTIN DEPOLYMERIZING FACTOR 9 controls development and gene expression in Arabidopsis. Plant Mol Biol 68:619–632 Castano E, Philimonenko VV, Kahle M, Fukalová J, Kalendová A, Yildirim S, Dzijak R,Dingová-Krásna H, Hozák P (2012) Actin complexes in the cell nucleus: new stones in an old field. Histochem Cell Biol 133:607–626 Chen CW, Yang YW, Lur HS, Tsai YG, Chang MC (2006) A novel function of abscisic acid in regulation of rice (Oryza sativa L.) roots growth and development Plant Cell and Physiol 47:1–13 Clement M, Tijs K, Natalia R, Mohamed YB, Andrei S, Gilbert E, Pierre A, Patrick JH, de Janice AE (2009) Actin-depolymerizing factor 2-mediated actin dynamics are essential for root-knot nematode infection of Arabidopsis. Plant Cell 21:2963–2979 Drobak BK, Franklin-Tong VE, Staiger CT (2004) The role of the actin cytoskeleton in plant cell signaling. New Phytol 163:13–30 Egierszdorff S, Kacperska A (2001) Low temperature effects on growth and actin cytoskeleton organization in suspension cells of winter oilseed rape. Plant Cell Tissue Organ Cult 65:149–158 Engler JA, Rodiuc N, Smertenko A, Abad P (2010) Plant actin cytoskeleton re-modeling by plant parasitic nematodes. Plant Signal Behav 5:213–217 Feng Y, Liu Q, Xue Q (2006) Comparative study of rice and Arabidopsis actin depolymerizing factors gene families. J Plant Physiol 163:69–79 Hussey PJ, Ketelaar T, Deeks MJ (2006) Control of the actin cytoskelton in plant cell growth.Annu Rev Plant Biol 57:109–125 Jeong YM, Jung EJ, Hwang HJ, Kim H, Lee SY, Kim SG (2009) Roles of the first intron on the expression of Arabidopsis (Arabidopsis thaliana) genes for actin and actin-binding proteins. Plant Sci 176:58–65 Jiang CJ, Weeds AG, Hussey PJ (1997) The maize actin-depolymerizing factor, ZmADF3,redistributes to the growing tip of elongating root hairs and can be induced to translocate into the nucleus with actin. Plant J 12:1035–1043 Kang JY, Choi HI, Im MY, Kim SY (2002) Arabidopsis basic leucine zipper proteins that mediate stress-responsive abscisic acid signaling. Plant Cell 14:343–357 Li XB, Xu D, Wang XL, Huang GQ, Luo J, Li DD, Zhang ZT, Xu WL (2010) Three cotton genes preferentially expressed in flower tissues encoding actin-depolymerizing factors which are involved in F-actin dynamics in cells. J Exp Bot 61:41–53 Liu SG, Zhu DZ, Chen GH, Gao XQ, Zhang XS (2012) Disrupted actin dynamics trigger an increment in the reactive oxygen species levels in the Arabidopsis root under salt stress. Plant Cell Report doi:10.1007/s00299-012-1242-z Livak JK, Schnittgen TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2 -ΔΔCt method. Methods 25:402–408 Lopez I, Anthony RG, Maciver SK, Jiang CJ, Khan S, Weeds AG, Hussey PJ (1996) Pollen specific expression of maize genes encoding actin depolymerizing factor-like proteins. Proc Natl Acad Sci USA 93:7415–7420 Lu B, Gong ZG, Wang J, Zhang JH, Liang JS (2007) Microtubule dynamics in relation to osmotic stress-induced ABA accumulation in Zea mays roots. J Exp Bot 58:2565–2572 Maciver SK, Hussey PJ (2002) The ADF/cofilin family: actin-remodeling proteins. Genome Biol 3:12, reviews3007.1–3007 Malerba M, Crosti P, Cerana R (2010) Effect of heat stress on actin cytoskelton and endoplasmic reticulum of tobacco BY-2 cultured cells and its inhibition by Co2+. Protoplasma 239:23–30 Miklis M, Consonni C, Bhat RA, Lipka V, Schulze-Lefert P, Panstruga R (2007) Barley MLO modulates actin-dependent and actin-independent antifungal defense pathways at the cell periphery. Plant Physiol 144:1132–1143 Mun JH, Lee SY, Yu HJ, Jeong YM, Shin MY, Kim H, Lee I, Kim SG (2002) Petunia actin depolymerizing factor is mainly accumulated in vascular tissue and its gene expression is enhanced by the first intron. Gene 292:233–243 Narusaka Y, Nakashima K, Shinwari ZK, Sakuma Y, Furihata T, Abe H, Narusaka M,Shinozaki K, Yamaguchi-Shinozaki K (2003) Interaction between two cis-acting elements,ABRE and DRE, in ABA-dependent expression of Arabidopsis Rd29A gene in response to dehydration and high-salinity stress. Plant J 34:137–148 Nick P (2008) Plant Microtubules. In: Nick P (ed) Microtubules as Sensors for Abiotic Stimuli, 2nd edn. Springer, Berlin Heidelberg Ouellet F, Carpentier E, Cope MJ, Monroy AF, Sarhan F (2001) Regulation of a wheat actin depolymerizing factor during cold acclimation. Plant Physiol 12:360–368 Rando OJ, Zhao K, Crabtree CR (2000) Searching for a function for nuclear actin. Trends Cell Biol 10:92–97 Ruzicka DR, Kandasamy MK, McKinney EC, Burgos-Rivera B, Meagher RB (2007) The ancient subclasses of Arabidopsis Actin Depolymerizing Factor genes exhibit novel and differential expression. Plant J 52:460–472 Sakumaa Y, Maruyamaa K, Osakabea Y, Qina F, Sekib M, Shinozaki K, Yamaguchi-Shinozakia K (2006) Functional analysis of an Arabidopsis transcription factor, DREB2A,involved in drought-responsive gene expression. Plant Cell 18:1292–1309 Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002a) Proteomic analysis of rice leaves during drought stress and recovery. Proteomics 2:1131–1145 Salekdeh GH, Siopongco J, Wade LJ, Ghareyazie B, Bennett J (2002b) A proteomic approach to analyzing drought- and salt-responsiveness in rice. Field Crop Res 76:199–219 Smertenko AP, Jiang CJ, Simmons NJ, Weeds AG, Davies DR, Hussey PJ (1998) Ser6 in the maize actin-depolymerizing factor, ZmADF3, is phosphorylated by a calcium-stimulated protein kinase and is essential for the control of functional activity. Plant J 14:187–193 Solanke AU, Sharma AK (2008) Signal transduction during cold stress in plants. Physiol Mol Biol Plants 14:69–79 Staiger CJ, Gibbon BC, Kovar DR, Zonia LE (1997) Profilin and actin-depolymerizing factor: modulators of actin organization in plants. Trends Plant Sci 2:275–281 Staiger CJ, Blanchoin L (2006) Actin dynamics: old friends with new stories. Curr Opin Plant Biol 9:554–562 Tian M, Chaudhry F, Ruzicka DR, Meagher RB, Staiger CJ, Day B (2009) Arabidopsis actin depolymerizing factor AtADF4 mediates defense signal transduction triggered by the Pseudomonas syringae effector AvrPphB. Plant Physiol 150:815–824 Vartiainen MK (2008) Nuclear actin dynamics-From form to function. FEBS Lett 582:2033–2040 Vidali L, Augustine RC, Fay SN, Franco P, Pattavina KA, Bezanilla M (2009) Rapid screening for temperature-sensitive alleles in plants. Plant Physiol 151:506–514 Wang C, Zhang L, Yuan M, Ge Y, Liu Y, Fan J, Ruan Y, Cui Z, Tong S, Zhang S (2010) The microfilament cytoskeleton plays a vital role in salt and osmotic stress tolerance in Arabidopsis. Plant Biol 12:70–78 Wang C, Zhang L, Huang RD (2011) Cytoskeleton and plant salt stress tolerance. Plant Signal Behav 6:29–31 Wang HY, Wang J, Gao P, Jiao GL, Zhao PM, Li Y, Wang GL, Xia GX (2009) Downregulation of GhADF1 gene expression affects cotton fiber properties. Plant Biotechnol J 7:13–23 Yan S, Tang Z, Su W, Sun W (2005) Proteomic analysis of salt stress-responsive proteins in rice root. Proteomics 5:235–244 Yang L, Zheng B, Mao C, Yi K, Liu F, Wu Y, Tao Q, Wu P (2003) cDNA-AFLP analysis of inducible gene expression in rice seminal root tips under a water deficit. Gene 314:141–148 | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62893 | - |
dc.description.abstract | 研究水稻根系結構與環境間的影響,對於水稻的生長與發育很重要。環境逆境會導致水稻根部發生形態改變現象,先前文獻也證實外加離層酸會促使水稻根尖部位發生膨大、且根毛增生以及發生側根原基的形成。為了瞭解離層酸對水稻根尖形態改變之影響以及與非生物性逆境間的關聯性,本論文以外加10 μM離層酸處理0、6、12與24小時之TCN1水稻根尖RNA進行NSF45K之微陣列分析。總共篩選出917個離層酸調控表現具顯著差異性的基因探針數。GO enrichment分析顯示與氧化逆境、蛋白質水解及脂質運送有關。KO enrichment分析顯示極長鏈脂肪酸延長、黃酮類生合成、與醣解作用相關路徑的酵素基因具顯著誘導表現。此外,涉及脂質運送、細胞壁修飾與氧化逆境反應相關之酵素基因群亦具有顯著性表現。超氧陰離子自由基與過氧化氫的原位螢光染色結果,說明活化氧族可能參與水稻根尖於離層酸作用下的生長分化過程。而外加離層酸也造成根尖荷爾蒙代謝與訊息傳導相關基因群表現的顯著性改變。水稻根尖轉錄體之微陣列分析指出離層酸可誘導水稻根尖形態的變化以適應環境變動。除此之外,於水稻根尖受離層酸調控差異性表現之基因群中,發現肌動蛋白去聚合因子基因,OsADF3,表現量增加且亦受乾旱及高鹽逆境所影響。肌動蛋白去聚合因子(Actin Depolymerizing Factor, ADFs)為參與細胞骨架動態變化相關之肌動結合蛋白。ADFs已知與植物細胞之形狀、分裂,生長發育、訊息傳遞及生物性逆境相關。但ADFs是否參與植物之非生物性逆境耐受性及其功能則仍有待探討。本論文首先以RT-PCR分析水稻OsADF家族的基因表現,結果指出OsADF家族各成員於不同的組織、發育期及非生物性逆境處理下之基因表現皆具差異性。OsADF1與OsADF3之GFP融合蛋白表現於細胞核中。啟動子OsADF1與OsADF3::GUS活性皆表現於維管束組織。在非生物性逆境與ABA處理下,啟動子OsADF3 :: GUS活性誘導表現於側根和根尖。異位過度表現OsADF3可增加阿拉伯芥轉殖株對於滲透壓及乾旱逆境的耐受性。在添加不同濃度甘露醇培養基中,阿拉伯芥OsADF3-OE轉殖株的萌芽率高於野生型,且轉殖株幼苗根長比野生型不受甘露醇影響抑制生長。乾旱逆境下OsADF3-OE轉殖株比野生型具存活率,且轉殖株比野生型顯著被誘導與耐旱反應相關之基因表現(RD22, ABF4, DREB2A, RD29A, PIP1; 4 and PIP2; 6)。這些結果指出OsADF3基因可能參與植物逆境反應或耐受性。總而言之,本研究以微陣列分析探討了離層酸於水稻根尖形態變化上扮演之可能角色;亦對OsADF3與非生物性逆境之關連提出了佐證。 | zh_TW |
dc.description.abstract | The establishment of root architecture in response to environmental stresses is important for rice growth and development. Environmental stresses caused rice root tips morphological alteration. Exogenously applied abscisic acid has been shown to cause rice root tips swollen, root hair and lateral root formation. To understand the effect of ABA on the alteration of rice root architecture, we conducted a NSF45K microarray analysis for a large-scale gene expression profiling with 10 μM ABA-treated rice root tip at different time points (0, 6, 12 and 24hr). More than 917 differentially expressed probes which affected by ABA were identified. Transcriptome analyses of ABA-treated TCN1 rice root tips through Gene Ontology (GO) enrichment analysis showed that those genes involved in very long-chain fatty acid elongation, flavonoid biosynthesis, and glycolysis are significantly induced. Moreover, the transcripts of genes that predominantly participated in lipid transport, cell wall modification, antioxidants and protective enzymes were also increased. The in situ staining of superoxide and hydrogen peroxide in root tip after ABA treatment suggesting that ROS may play a role in adjusting the growth and development of rice root. The gene expression patterns of phytohormones biosynthesis and signaling were also changed. Taken together, these results provide an initial step to understand how ABA leads to the morphological and physiological changes of rice root. Besides, microarray analysis pointed out OsADF3 gene was induced by ABA and also regulated by drought and salt stresses. Actin depolymerizing factors (ADFs) are small actin-binding proteins that known to involve in plant growth, development and pathogen defense. However, little is known about the role of OsADFs under abiotic stresses. Therefore, to understand the physiological function of OsADF gene family, first we determined the gene expression profile of the OsADF gene family by RT-PCR. The OsADF genes showed distinct and overlapping gene expression patterns at different growth stages, tissues and abiotic stresses. We found that both OsADF1 and OsADF3 proteins were localized in the nucleus. OsADF1 and OsADF3::GUS activity were preferentially expressed in vascular tissues. Under ABA or abiotic stress treatments, OsADF3::GUS activity was induced in lateral roots and root tips. Ectopically overexpressed OsADF3 enhanced the mannitol- and drought-stress tolerance of transgenic Arabidopsis seedlings by increasing germination rate, root length and survival rate. Several drought-tolerance responsive genes (RD22, ABF4, DREB2A, RD29A, PIP1; 4 and PIP2; 6) were regulated in transgenic Arabidopsis under drought stress. These results suggested that OsADF3 may participate in plant abiotic stresses response or tolerance. In conclusion, within this study we highlighted the role of ABA in alteration of rice root tip change using microarray analysis and demonstrated the relationship of OsADF3 with drought stress tolerance. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:13:58Z (GMT). No. of bitstreams: 1 ntu-102-D95621103-1.pdf: 5058176 bytes, checksum: 1cf2ad81c38605a54af4b2e367730888 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 目錄 …………………………………………………………………………………..ii
圖表目錄 ……………………………………………………………………………..v 中文摘要 ……………………………………………………………………………..ix 英文摘要 …………………………………………………………………………......xi 縮寫字對照 ……………………………………………………………………….xiii 第一章 前言..………………………………...……………………………………… 1 一、前言.………………………………………………………………………… 2 二、本論文之試驗架構及意義.………………………………………………… 3 第二章 離層酸影響水稻根尖之轉錄體分析 ……...………………………………..5 一、前言 ….……………………………………………….………………....……6 二、材料與方法.………………………………………………………...……….10 1. 植株之種植與處理 ..………………………………………………….10 2. RNA製備和微陣列雜合 ……………………………………………..11 3. 微陣列數據處理和分析 ……………………………………………...12 4. 及時定量RT-PCR分析 ………………………………………………13 5. 脂質,黃酮類與H2O2及O2.-染色分析 ………………………….…14 6. 根尖組織的顯微切片分析 ….…………………………………………15 7. 水稻根尖之面積測定 ………………………………………………….15 8. 統計分析 ……………………………………………………………….16 三、結果………………………………………………………………………….17 1. 水稻根尖於乾旱逆境下之形態改變……………….…………………17 2. 水稻根尖經離層酸處理下之微陣列分析 ………………………… 17 3. 水稻根尖受離層酸調控差異性表現基因群之GO enrichment analysis ………………………………………………….18 4. 水稻根尖受離層酸調控差異性表現基因群參與 生化途徑之分析……………………………………………....……….19 5. 水稻根尖受離層酸誘導特異性表現參與脂質運送、細胞壁修飾、 與氧化逆境反應相關酵素基因群之轉錄表現分析 …...……………21 6 水稻根尖之H2O2與O2.-原位螢光染色分析 ……...……..…………23 7. 水稻根尖經離層酸處理下之荷爾蒙代謝與訊息傳遞相關基因 之轉錄表現分析...……………………………………………………..23 8. 水稻根尖受離層酸調控差異性表現與根尖延長部特異性表現之 基因群enrichment analysis………….……………..………………….25 四、討論 …………………………………………..…………………………...26 1. 水稻根尖經離層酸處理造成的形態改變與非生物性逆境之關連….26 2. 水稻根尖經離層酸處理之基因轉錄變化與水稻遭遇非生物性逆境 的關係……………………………………………………….…………26 3. 離層酸調控根尖差異性表現基因群所參與的生化途徑與水稻 根尖形態改變的關係….………………………………………………27 4. 參與脂質運送、細胞壁修飾與氧化逆境反應相關酵素差異性表現之 基因群影響水稻根尖形態改變,和非生物性逆境的關係……….…29 5. 荷爾蒙代謝及訊息傳遞相關基因的表現變化對於水稻根部的影響.. ………….………………………………………………………………30 6. 離層酸影響水稻根尖之可能分子調控機制 …………...……………31 五、參考文獻 ………………………………………………………….………32 第三章 水稻肌動蛋白去聚合因子基因家族表現分析 與OsADF3基因功能性研究 ………………………………………..…….77 一、前言 …………………………………………………………………………78 二、材料與方法 ……..….……………...……………………………….………81 1. 植株之種植與處理..…….………………………………………..……81 2. 水稻肌動蛋白去聚合因子家族基因之生物資訊分析 ..….…………83 3. 水稻肌動蛋白去聚合因子家族基因表現分析………..….……..……83 4. 構築植物基因轉殖表現載體 ….………………………………..……85 5. 轉殖載體之大量純化抽取 ….…………………………………..……87 6. 融合蛋白之次細胞位置分析 …..……………………………….....…87 7. 水稻與阿拉伯芥基因轉殖植物的產生 ……………………..……….88 8. 目標基因啟動子的特性分析 ………………………………..……….90 9. 水稻組織的顯微切片 ………………………………………..……….90 10. 統計分析………………………………………………………..……...91 三、結果 ……….....………………………………………………………..……92 1. OsADF家族基因於不同的組織、發育期及非生物性逆境處理下 之基因表現分析…….....………………………………………..……92 2. OsADF1與OsADF3之GFP融合蛋白次細胞表現位置分析 …………………………………………………………………..….93 3. OsADF1與OsADF3之啟動子GUS活性分析.…………………..….93 4. OsADF3-OE阿拉伯芥轉殖株之分子鑑定與功能分析…………..….94 5. 乾旱逆境下OsADF3-OE阿拉伯芥轉殖株與野生型之耐旱反應 相關基因表現分析 …..………………..…………..………....…….…95 四、討論 …….…………………………………………………………..………96 1. 水稻OsADF家族基因之起動子順式作用區預測與 微陣列資訊分析 ...……………………………………………..……96 2. 雙單子葉植物之肌動蛋白去聚合因子家族的親緣演化與基因表現 分析 ...……………………………………………………………..…97 3. 水稻OsADF1-GFP與OsADF3-GFP融合蛋白表現 位於細胞核……………………………………………….……...….....98 4. 水稻OsADF3顯著性表現於維管束組織,可能與植物遭受乾旱逆境反應或耐受性有關 ...………………………………………………....98 五、參考文獻 …………………………………………………………...……100 第四章 結論...…………………………………...……..………………….……..…127 圖表目錄 表目錄 表2-1、 水稻根尖受ABA調控差異性表現基因群之GO enrichment analysis ……39 表2-2、 水稻根尖受ABA調控差異性表現與非生物性逆境調控表現 基因群之enrichment analysis .……………………………………..…..…40 表2-3、 水稻根尖受ABA調控差異性表現基因群之KO (KEGG Orthology) enrichment analysis …………………………………………………………41 表2-4、 水稻根尖受ABA調控差異性表現基因群參與極長鏈脂肪酸延長、黃酮 類生合成、與醣解作用之微陣列基因轉錄變化量 ..…………………….42 表2-5、 水稻根尖受ABA調控差異性表現特定基因群之GO enrichment analysis …………………………………………………………….………………43 表2-6、 水稻根尖受ABA調控差異性表現參與脂質運送、細胞壁修飾、氧化逆境反應相關酵素及細胞骨架變化之基因群與非生物性逆境的關連性.....…44 表2-7、 水稻根尖受ABA調控差異性表現基因群參與脂質運送、細胞壁修飾,氧化逆境反應相關酵素及細胞骨架變化之微陣列基因轉錄變化量……….46 表2-8、 水稻根尖受ABA調控差異性表現基因群與荷爾蒙代謝與訊息傳遞 相關之GO enrichment analysis……………………………………..………48 表2-9、 水稻根尖受ABA調控差異性表現基因群參與荷爾蒙代謝與訊息傳遞 相關之微陣列基因轉錄變化量……………………………………….……49 表2-10、水稻根尖受ABA調控差異性表現與根尖延長部特異性表現 基因群之enrichment analysis…………………..……………………...……51 表2-11、水稻根尖轉錄體分析ABA調控差異性表現基因之引子對……..…..……52 表3-1、 水稻肌動蛋白去聚合因子(OsADFs)家族成員與其基因結構………...…105 表3-2、 水稻肌動蛋白去聚合因子基因家族成員RT-PCR所使用的專一性引子對 ...……………………………………………………………………………106 表3-3、 阿拉伯芥非生物性逆境相關之基因Realtime-PCR所使用的專一性 引子對 ….…………………………………………………………………107 圖目錄 圖2-1、 水稻根尖於乾旱逆境下之形態改變 ..……………………………………55 圖2-2、 水稻根尖經外加離層酸後之形態改變 ..…………………………………57 圖2-3、 以不同濃度離層酸處理水稻之根尖形態變化觀察 ………….…….……59 圖2-4、 水稻根尖經10 μM 離層酸處理下之微陣列分析 .……..……………….61 圖2-5、 以real time-PCR進行轉錄體數據有效性之驗證 .………………………63 圖2-6、 離層酸處理下水稻根尖極長鏈脂肪酸延長反應相關基因的表現與 脂質染色之根尖橫切面觀察 ….………………….…………….……….64 圖2-7、 離層酸處理下水稻根尖黃酮類生合成反應相關基因的表現與 黃酮類染色之根尖定量分析………………………………………………66 圖2-8、 離層酸處理下水稻根尖醣解作用相關途徑基因的 轉錄表現分析….…………………………………………………………...68 圖2-9、 水稻根尖受離層酸調控差異性表現參與脂質運送、細胞壁修飾,氧化 逆境反應相關酵素與細胞骨架變化之基因群的轉錄表現分析…………71 圖2-10、水稻根尖經離層酸處理下之H2O2及O2.-原位螢光染色分析..…………73 圖2-11、水稻根尖經離層酸處理下之荷爾蒙代謝與訊息傳遞相關基因 之轉錄表現分析...………………….………………………………………74 圖3-1、 水稻與阿拉伯芥肌動蛋白去聚合因子基因家族胺基酸的 序列比對……………………………………………………………...108 圖3-2、 水稻與阿拉伯芥肌動蛋白去聚合因子基因家族之 親緣演化樹分析………………………………………………………..….110 圖3-3、 水稻肌動蛋白去聚合因子基因家族啟動子之順式作用DNA序列預測 與其各基因成員於不同非生物性逆境之誘導表現分析...........................112 圖3-4、 水稻肌動蛋白去聚合因子家族基因於不同組織與生長期之基因轉錄 表現分析.......................................................................................................114 圖3-5、 水稻肌動蛋白去聚合因子家族基因於非生物性逆境與離層酸處理下 誘導之基因轉錄表現分析...........................................................................116 圖3-6、 OsADF1-GFP與OsADF3融合蛋白次細胞位置分析…….……..………118 圖3-7、 轉殖水稻OsADF1啟動子::GUS各部位組織之GUS染色 活性分析.......................................................................................................119 圖3-8、 轉殖水稻OsADF3啟動子::GUS各部位組織之GUS染色 活性分析.......................................................................................................120 圖3-9、 轉殖水稻OsADF3啟動子::GUS之幼苗根部(T1)經非生物性逆境與ABA 處理後之GUS染色活性分析.....................................................................121 圖3-10、阿拉伯芥OsADF3-OE轉殖株的分子鑑定與功能性分析………………122 圖3-11、阿拉伯芥OsADF3-OE轉殖株對於甘露醇的耐受性及外表型分析....124 圖3-12、以Real-time PCR分析阿拉伯芥OsADF3-OE轉殖株於乾旱逆境 相關基因之表現......…………………………....………………………….126 附錄目錄 附錄3-1、木村氏水耕液(Kimura solution)配方 ………………….………… 131 附錄3-2、基因轉殖表現載體構築之圖譜 ................………………………… 132 附錄3-3、水稻基因轉殖用培養基列表 ……………………………………… 133 附錄3-4、阿拉伯芥基因轉殖用培養基列表 ………………………………… 135 附錄3-5、GUS staining solution配方 ………………………………………… 136 | |
dc.language.iso | zh-TW | |
dc.title | 離層酸處理水稻根尖轉錄體及水稻肌動蛋白去聚合因子基因家族之功能性分析 | zh_TW |
dc.title | Functional Analysis of Abscisic Acid-Treated Rice Root Tip Transcriptome and Rice Actin Depolymerizing Factor (OsADF) Gene Family | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-1 | |
dc.description.degree | 博士 | |
dc.contributor.oralexamcommittee | 吳素幸(Shu-Hsing Wu),鄭萬興(Wan-Hsing Cheng),洪傳揚(Chwan-Yang Hong),靳宗洛(Tsung-Luo Jinn),黃鵬林(Pung-Ling Huang) | |
dc.subject.keyword | 水稻, 根尖組織, 離層酸, 基因表現, 生長與分化, 肌動蛋白去聚合因子, 基因槍, GUS染色, 異源基因表現, 非生物性逆境, | zh_TW |
dc.subject.keyword | Rice (Oryza sativa L.), root tips, Abscisic Acid, Gene expression profiling, development and growth, Actin depolymerizing factor, Particle bombardment, GUS staining, Heterologus gene expression, Abiotic stresses, | en |
dc.relation.page | 136 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-02-07 | |
dc.contributor.author-college | 生物資源暨農學院 | zh_TW |
dc.contributor.author-dept | 農藝學研究所 | zh_TW |
顯示於系所單位: | 農藝學系 |
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