請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62536
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 鄭謙仁(Chian-Ren Jeng) | |
dc.contributor.author | Tzu-Ting Hsu | en |
dc.contributor.author | 徐子婷 | zh_TW |
dc.date.accessioned | 2021-06-16T16:04:00Z | - |
dc.date.available | 2014-07-08 | |
dc.date.copyright | 2013-07-08 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-06-28 | |
dc.identifier.citation | 邱明堂、鐘文彬、鄭謙仁、闕玲玲、龐飛。本省病死保育和肥育豬隻肺炎及肺臟內豬繁殖與呼吸道症侯群病毒感染狀況。中華獸醫誌 23: 430-440, 1997
Albina, E., 1997. Epidemiology of porcine reproductive and respiratory syndrome (PRRS): an overview. Vet Microbiol 55, 309-316. Ansari, I.H., Kwon, B., Osorio, F.A., Pattnaik, A.K., 2006. Influence of N-linked glycosylation of porcine reproductive and respiratory syndrome virus GP5 on virus infectivity, antigenicity, and ability to induce neutralizing antibodies. J Virol 80, 3994-4004. Bautista, E.M., Faaberg, K.S., Mickelson, D., McGruder, E.D., 2002. Functional properties of the predicted helicase of porcine reproductive and respiratory syndrome virus. Virology 298, 258-270. Bautista, E.M., Molitor, T.W., 1997. Cell-mediated immunity to porcine reproductive and respiratory syndrome virus in swine. Viral Immunol 10, 83-94. Bautista, E.M., Suarez, P., Molitor, T.W., 1999. T cell responses to the structural polypeptides of porcine reproductive and respiratory syndrome virus. Arch Virol 144, 117-134. Beura, L.K., Sarkar, S.N., Kwon, B., Subramaniam, S., Jones, C., Pattnaik, A.K., Osorio, F.A., 2010. Porcine reproductive and respiratory syndrome virus nonstructural protein 1beta modulates host innate immune response by antagonizing IRF3 activation. J Virol 84, 1574-1584. Botner, A., Strandbygaard, B., Sorensen, K.J., Have, P., Madsen, K.G., Madsen, E.S., Alexandersen, S., 1997. Appearance of acute PRRS-like symptoms in sow herds after vaccination with a modified live PRRS vaccine. Vet Rec 141, 497-499. Calvert, J.G., Slade, D.E., Shields, S.L., Jolie, R., Mannan, R.M., Ankenbauer, R.G., Welch, S.K., 2007. CD163 expression confers susceptibility to porcine reproductive and respiratory syndrome viruses. J Virol 81, 7371-7379. Cancel-Tirado, S.M., Evans, R.B., Yoon, K.J., 2004. Monoclonal antibody analysis of porcine reproductive and respiratory syndrome virus epitopes associated with antibody-dependent enhancement and neutralization of virus infection. Vet Immunol Immunopathol 102, 249-262. Cano, J.P., Dee, S.A., Murtaugh, M.P., Pijoan, C., 2007a. Impact of a modified-live porcine reproductive and respiratory syndrome virus vaccine intervention on a population of pigs infected with a heterologous isolate. Vaccine 25, 4382-4391. Cano, J.P., Dee, S.A., Murtaugh, M.P., Trincado, C.A., Pijoan, C.B., 2007b. Effect of vaccination with a modified-live porcine reproductive and respiratory syndrome virus vaccine on dynamics of homologous viral infection in pigs. Am J Vet Res 68, 565-571. Cavanagh, D., 1997. Nidovirales: a new order comprising Coronaviridae and Arteriviridae. Arch Virol 142, 629-633. Chan, H.T., Chia, M.Y., Pang, V.F., Jeng, C.R., Do, Y.Y., Huang, P.L., 2013. Oral immunogenicity of porcine reproductive and respiratory syndrome virus antigen expressed in transgenic banana. Plant Biotechnol. J. 11, 315-324. Chen, Z., Lawson, S., Sun, Z., Zhou, X., Guan, X., Christopher-Hennings, J., Nelson, E.A., Fang, Y., 2010a. Identification of two auto-cleavage products of nonstructural protein 1 (nsp1) in porcine reproductive and respiratory syndrome virus infected cells: nsp1 function as interferon antagonist. Virology 398, 87-97. Chen, Z., Zhou, X., Lunney, J.K., Lawson, S., Sun, Z., Brown, E., Christopher-Hennings, J., Knudsen, D., Nelson, E., Fang, Y., 2010b. Immunodominant epitopes in nsp2 of porcine reproductive and respiratory syndrome virus are dispensable for replication, but play an important role in modulation of the host immune response. J Gen Virol 91, 1047-1057. Chia, M.Y., Hsiao, S.H., Chan, H.T., Do, Y.Y., Huang, P.L., Chang, H.W., Tsai, Y.C., Lin, C.M., Pang, V.F., Jeng, C.R., 2010. Immunogenicity of recombinant GP5 protein of porcine reproductive and respiratory syndrome virus expressed in tobacco plant. Vet Immunol Immunopathol 135, 234-242. Cho, J.G., Dee, S.A., Deen, J., Guedes, A., Trincado, C., Fano, E., Jiang, Y., Faaberg, K., Collins, J.E., Murtaugh, M.P., Joo, H.S., 2006. Evaluation of the effects of animal age, concurrent bacterial infection, and pathogenicity of porcine reproductive and respiratory syndrome virus on virus concentration in pigs. Am J Vet Res 67, 489-493. Collins, J.E., Benfield, D.A., Christianson, W.T., Harris, L., Hennings, J.C., Shaw, D.P., Goyal, S.M., McCullough, S., Morrison, R.B., Joo, H.S., et al., 1992. Isolation of swine infertility and respiratory syndrome virus (isolate ATCC VR-2332) in North America and experimental reproduction of the disease in gnotobiotic pigs. J Vet Diagn Invest 4, 117-126. Curtiss R. I. and Cardineau, G.A. 1990. Oral immunization by transgenic plants WO90/02484. Das, P.B., Dinh, P.X., Ansari, I.H., de Lima, M., Osorio, F.A., Pattnaik, A.K., 2010. The minor envelope glycoproteins GP2a and GP4 of porcine reproductive and respiratory syndrome virus interact with the receptor CD163. J Virol 84, 1731-1740. Darwich, L., Diaz, I., Mateu, E., 2010. Certainties, doubts and hypotheses in porcine reproductive and respiratory syndrome virus immunobiology. Virus Res 154, 123-132. Delputte, P.L., Costers, S., Nauwynck, H.J., 2005. Analysis of porcine reproductive and respiratory syndrome virus attachment and internalization: distinctive roles for heparan sulphate and sialoadhesin. J Gen Virol 86, 1441-1445. Diaz, I., Darwich, L., Pappaterra, G., Pujols, J., Mateu, E., 2005. Immune responses of pigs after experimental infection with a European strain of Porcine reproductive and respiratory syndrome virus. J Gen Virol 86, 1943-1951. Diaz, I., Pujols, J., Ganges, L., Gimeno, M., Darwich, L., Domingo, M., Mateu, E., 2009. In silico prediction and ex vivo evaluation of potential T-cell epitopes in glycoproteins 4 and 5 and nucleocapsid protein of genotype-I (European) of porcine reproductive and respiratory syndrome virus. Vaccine 27, 5603-5611. Done, S.H., Paton, D.J., White, M.E., 1996. Porcine reproductive and respiratory syndrome (PRRS): a review, with emphasis on pathological, virological and diagnostic aspects. Br Vet J 152, 153-174. Duan, X., Nauwynck, H.J., Favoreel, H., Pensaert, M.B., 1998a. Porcine reproductive and respiratory syndrome virus infection of alveolar macrophages can be blocked by monoclonal antibodies against cell surface antigens. Adv Exp Med Biol 440, 81-88. Duan, X., Nauwynck, H.J., Favoreel, H.W., Pensaert, M.B., 1998b. Identification of a putative receptor for porcine reproductive and respiratory syndrome virus on porcine alveolar macrophages. J Virol 72, 4520-4523. Duan, X., Nauwynck, H.J., Pensaert, M.B., 1997. Effects of origin and state of differentiation and activation of monocytes/macrophages on their susceptibility to porcine reproductive and respiratory syndrome virus (PRRSV). Arch Virol 142, 2483-2497. Faaberg, K.S., Hocker, J.D., Erdman, M.M., Harris, D.L., Nelson, E.A., Torremorell, M., Plagemann, P.G., 2006. Neutralizing antibody responses of pigs infected with natural GP5 N-glycan mutants of porcine reproductive and respiratory syndrome virus. Viral Immunol 19, 294-304. Fenaux, M., Halbur, P.G., Haqshenas, G., Royer, R., Thomas, P., Nawagitgul, P., Gill, M., Toth, T.E., Meng, X.J., 2002. Cloned genomic DNA of type 2 porcine circovirus is infectious when injected directly into the liver and lymph nodes of pigs: characterization of clinical disease, virus distribution, and pathologic lesions. J Virol 76, 541-551. Fischer, R., Emans, N., 2000. Molecular farming of pharmaceutical proteins. Transgenic Res 9, 279-299. Foss, D.L., Zilliox, M.J., Meier, W., Zuckermann, F., Murtaugh, M.P., 2002. Adjuvant danger signals increase the immune response to porcine reproductive and respiratory syndrome virus. Viral Immunol 15, 557-566. Gonin, P., Pirzadeh, B., Gagnon, C.A., Dea, S., 1999. Seroneutralization of porcine reproductive and respiratory syndrome virus correlates with antibody response to the GP5 major envelope glycoprotein. J Vet Diagn Invest 11, 20-26. Guo, A., Wu, G., Gong, W., Luo, X., Zheng, H., Jia, H., Cai, X., 2012. Outbreaks of highly pathogenic porcine reproductive and respiratory syndrome in Jiangxi province, China. Ir Vet J 65, 14. Halbur, P.G., Miller, L.D., Paul, P.S., Meng, X.J., Huffman, E.L., Andrews, J.J., 1995. Immunohistochemical identification of porcine reproductive and respiratory syndrome virus (PRRSV) antigen in the heart and lymphoid system of three-week-old colostrum-deprived pigs. Vet Pathol 32, 200-204. Halbur, P.G., Paul, P.S., Frey, M.L., Landgraf, J., Eernisse, K., Meng, X.J., Andrews, J.J., Lum, M.A., Rathje, J.A., 1996. Comparison of the antigen distribution of two US porcine reproductive and respiratory syndrome virus isolates with that of the Lelystad virus. Vet Pathol 33, 159-170. Halbur, P.G., Paul, P.S., Vaughn, E.M., Andrews, J.J., 1993. Experimental reproduction of pneumonia in gnotobiotic pigs with porcine respiratory coronavirus isolate AR310. J Vet Diagn Invest 5, 184-188. Jeong, H.J., Song, Y.J., Lee, S.W., Lee, J.B., Park, S.Y., Song, C.S., Ha, G.W., Oh, J.S., Oh, Y.K., Choi, I.S., 2010. Comparative measurement of cell-mediated immune responses of swine to the M and N proteins of porcine reproductive and respiratory syndrome virus. Clin Vaccine Immunol 17, 503-512. Jiang, P., Jiang, W., Li, Y., Wu, S., Xu, J., 2004. Humoral immune response induced by oral administration of S. typhimurium containing a DNA vaccine against porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 102, 321-328. Jiang, W., Jiang, P., Li, Y., Tang, J., Wang, X., Ma, S., 2006a. Recombinant adenovirus expressing GP5 and M fusion proteins of porcine reproductive and respiratory syndrome virus induce both humoral and cell-mediated immune responses in mice. Vet Immunol Immunopathol 113, 169-180. Jiang, W., Jiang, P., Wang, X., Li, Y., Du, Y., Wang, X., 2008. Enhanced immune responses of mice inoculated recombinant adenoviruses expressing GP5 by fusion with GP3 and/or GP4 of PRRS virus. Virus Res 136, 50-57. Jiang, Y., Fang, L., Xiao, S., Zhang, H., Pan, Y., Luo, R., Li, B., Chen, H., 2007. Immunogenicity and protective efficacy of recombinant pseudorabies virus expressing the two major membrane-associated proteins of porcine reproductive and respiratory syndrome virus. Vaccine 25, 547-560. Jiang, Y., Xiao, S., Fang, L., Yu, X., Song, Y., Niu, C., Chen, H., 2006b. DNA vaccines co-expressing GP5 and M proteins of porcine reproductive and respiratory syndrome virus (PRRSV) display enhanced immunogenicity. Vaccine 24, 2869-2879. Kimman, T.G., Cornelissen, L.A., Moormann, R.J., Rebel, J.M., Stockhofe-Zurwieden, N., 2009. Challenges for porcine reproductive and respiratory syndrome virus (PRRSV) vaccinology. Vaccine 27, 3704-3718. Labarque, G., Reeth, K.V., Nauwynck, H., Drexler, C., Van Gucht, S., Pensaert, M., 2004. Impact of genetic diversity of European-type porcine reproductive and respiratory syndrome virus strains on vaccine efficacy. Vaccine 22, 4183-4190. Li, Y., Wang, X., Bo, K., Wang, X., Tang, B., Yang, B., Jiang, W., Jiang, P., 2007. Emergence of a highly pathogenic porcine reproductive and respiratory syndrome virus in the Mid-Eastern region of China. Vet J 174, 577-584. Lopez Fuertes, L., Domenech, N., Alvarez, B., Ezquerra, A., Dominguez, J., Castro, J.M., Alonso, F., 1999. Analysis of cellular immune response in pigs recovered from porcine respiratory and reproductive syndrome infection. Virus Res 64, 33-42. Mason, H.S., Warzecha, H., Mor, T., Arntzen, C.J., 2002. Edible plant vaccines: applications for prophylactic and therapeutic molecular medicine. Trends Mol Med 8, 324-329. Mateu, E., Diaz, I., 2008. The challenge of PRRS immunology. Vet J 177, 345-351. Meier, W.A., Galeota, J., Osorio, F.A., Husmann, R.J., Schnitzlein, W.M., Zuckermann, F.A., 2003. Gradual development of the interferon-gamma response of swine to porcine reproductive and respiratory syndrome virus infection or vaccination. Virology 309, 18-31. Meng, X.J., Paul, P.S., Halbur, P.G., Lum, M.A., 1995. Phylogenetic analyses of the putative M (ORF 6) and N (ORF 7) genes of porcine reproductive and respiratory syndrome virus (PRRSV): implication for the existence of two genotypes of PRRSV in the U.S.A. and Europe. Arch Virol 140, 745-755. Mengeling, W.L., Lager, K.M., Vorwald, A.C., Clouser, D.F., 2003. Comparative safety and efficacy of attenuated single-strain and multi-strain vaccines for porcine reproductive and respiratory syndrome. Vet Microbiol 93, 25-38. Meulenberg, J.J., Hulst, M.M., de Meijer, E.J., Moonen, P.L., den Besten, A., de Kluyver, E.P., Wensvoort, G., Moormann, R.J., 1993. Lelystad virus, the causative agent of porcine epidemic abortion and respiratory syndrome (PEARS), is related to LDV and EAV. Virology 192, 62-72. Meulenberg, J.J., Petersen den Besten, A., de Kluyver, E., van Nieuwstadt, A., Wensvoort, G., Moormann, R.J., 1997. Molecular characterization of Lelystad virus. Vet Microbiol 55, 197-202. Murtaugh, M.P., Elam, M.R., Kakach, L.T., 1995. Comparison of the structural protein coding sequences of the VR-2332 and Lelystad virus strains of the PRRS virus. Arch Virol 140, 1451-1460. Murtaugh, M.P., Xiao, Z., Zuckermann, F., 2002. Immunological responses of swine to porcine reproductive and respiratory syndrome virus infection. Viral Immunol 15, 533-547. Nauwynck, H.J., Duan, X., Favoreel, H.W., Van Oostveldt, P., Pensaert, M.B., 1999. Entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages via receptor-mediated endocytosis. J Gen Virol 80 ( Pt 2), 297-305. Nedialkova, D.D., Ulferts, R., van den Born, E., Lauber, C., Gorbalenya, A.E., Ziebuhr, J., Snijder, E.J., 2009. Biochemical characterization of arterivirus nonstructural protein 11 reveals the nidovirus-wide conservation of a replicative endoribonuclease. J Virol 83, 5671-5682. Nelsen, C.J., Murtaugh, M.P., Faaberg, K.S., 1999. Porcine reproductive and respiratory syndrome virus comparison: divergent evolution on two continents. J Virol 73, 270-280. Nelson, E.A., Christopher-Hennings, J., Benfield, D.A., 1994. Serum immune responses to the proteins of porcine reproductive and respiratory syndrome (PRRS) virus. J Vet Diagn Invest 6, 410-415. Neumann, E.J., Kliebenstein, J.B., Johnson, C.D., Mabry, J.W., Bush, E.J., Seitzinger, A.H., Green, A.L., Zimmerman, J.J., 2005. Assessment of the economic impact of porcine reproductive and respiratory syndrome on swine production in the United States. J Am Vet Med Assoc 227, 385-392. Ni, J., Yang, S., Bounlom, D., Yu, X., Zhou, Z., Song, J., Khamphouth, V., Vatthana, T., Tian, K., 2012. Emergence and pathogenicity of highly pathogenic Porcine reproductive and respiratory syndrome virus in Vientiane, Lao People's Democratic Republic. J Vet Diagn Invest 24, 349-354. Oleksiewicz, M.B., Botner, A., Toft, P., Normann, P., Storgaard, T., 2001. Epitope mapping porcine reproductive and respiratory syndrome virus by phage display: the nsp2 fragment of the replicase polyprotein contains a cluster of B-cell epitopes. J Virol 75, 3277-3290. Osorio, F.A., Galeota, J.A., Nelson, E., Brodersen, B., Doster, A., Wills, R., Zuckermann, F., Laegreid, W.W., 2002. Passive transfer of virus-specific antibodies confers protection against reproductive failure induced by a virulent strain of porcine reproductive and respiratory syndrome virus and establishes sterilizing immunity. Virology 302, 9-20. Ostrowski, M., Galeota, J.A., Jar, A.M., Platt, K.B., Osorio, F.A., Lopez, O.J., 2002. Identification of neutralizing and nonneutralizing epitopes in the porcine reproductive and respiratory syndrome virus GP5 ectodomain. J Virol 76, 4241-4250. Pirzadeh, B., Dea, S., 1997. Monoclonal antibodies to the ORF5 product of porcine reproductive and respiratory syndrome virus define linear neutralizing determinants. J Gen Virol 78 ( Pt 8), 1867-1873. Pirzadeh, B., Dea, S., 1998. Immune response in pigs vaccinated with plasmid DNA encoding ORF5 of porcine reproductive and respiratory syndrome virus. J Gen Virol 79 ( Pt 5), 989-999. Plagemann, P.G., 2004a. GP5 ectodomain epitope of porcine reproductive and respiratory syndrome virus, strain Lelystad virus. Virus Res 102, 225-230. Plagemann, P.G., 2004b. The primary GP5 neutralization epitope of North American isolates of porcine reproductive and respiratory syndrome virus. Vet Immunol Immunopathol 102, 263-275. Plagemann, P.G., Rowland, R.R., Faaberg, K.S., 2002. The primary neutralization epitope of porcine respiratory and reproductive syndrome virus strain VR-2332 is located in the middle of the GP5 ectodomain. Arch Virol 147, 2327-2347. Reed L.J and Muench H., 1938. A simple method of estimating fifty percent endpoints. Am. J. Hygiene 27, 493–497 Rossow, K.D., Benfield, D.A., Goyal, S.M., Nelson, E.A., Christopher-Hennings, J., Collins, J.E., 1996a. Chronological immunohistochemical detection and localization of porcine reproductive and respiratory syndrome virus in gnotobiotic pigs. Vet Pathol 33, 551-556. Rossow, K.D., Collins, J.E., Goyal, S.M., Nelson, E.A., Christopher-Hennings, J., Benfield, D.A., 1995. Pathogenesis of Porcine Reproductive and Respiratory Syndrome Virus Infection in Gnotobiotic Pigs. Vet Pathol 32, 361-373. Rossow, K.D., Laube, K.L., Goyal, S.M., Collins, J.E., 1996b. Fetal microscopic lesions in porcine reproductive and respiratory syndrome virus-induced abortion. Vet Pathol 33, 95-99. Royaee, A.R., Husmann, R.J., Dawson, H.D., Calzada-Nova, G., Schnitzlein, W.M., Zuckermann, F.A., Lunney, J.K., 2004. Deciphering the involvement of innate immune factors in the development of the host response to PRRSV vaccination. Vet Immunol Immunopathol 102, 199-216. Sanchez, C., Domenech, N., Vazquez, J., Alonso, F., Ezquerra, A., Dominguez, J., 1999. The porcine 2A10 antigen is homologous to human CD163 and related to macrophage differentiation. J Immunol 162, 5230-5237. Shen, G., Jin, N., Ma, M., Jin, K., Zheng, M., Zhuang, T., Lu, H., Zhu, G., Jin, H., Jin, M., Huo, X., Qin, X., Yin, R., Li, C., Li, H., Li, Y., Han, Z., Chen, Y., Jin, M., 2007. Immune responses of pigs inoculated with a recombinant fowlpox virus coexpressing GP5/GP3 of porcine reproductive and respiratory syndrome virus and swine IL-18. Vaccine 25, 4193-4202. Shimizu, M., Yamada, S., Murakami, Y., Morozumi, T., Kobayashi, H., Mitani, K., Ito, N., Kubo, M., Kimura, K., Kobayashi, M., et al., 1994. Isolation of porcine reproductive and respiratory syndrome (PRRS) virus from Heko-Heko disease of pigs. J Vet Med Sci 56, 389-391. Snijder, E.J., Meulenberg, J.J., 1998. The molecular biology of arteriviruses. J Gen Virol 79 ( Pt 5), 961-979. Spilman, M.S., Welbon, C., Nelson, E., Dokland, T., 2009. Cryo-electron tomography of porcine reproductive and respiratory syndrome virus: organization of the nucleocapsid. J Gen Virol 90, 527-535. Stevenson, G.W., Van Alstine, W.G., Kanitz, C.L., 1994. Characterization of infection with endemic porcine reproductive and respiratory syndrome virus in a swine herd. J Am Vet Med Assoc 204, 1938-1942. Stockhofe-Zurwieden, N., Navarro Camarro, J.A., Grosse-Beilage, E., Chavez, J., Pohlenz, J., 1993. Uterine and placental alterations in pregnant sows associated with the porcine epidemic abortion and respiratory syndrome (PEARS). Zentralblatt fur Veterinarmedizin. Reihe B. Zentralbl Veterinarmed B 40, 261-271. Storgaard, T., Oleksiewicz, M., Botner, A., 1999. Examination of the selective pressures on a live PRRS vaccine virus. Arch Virol 144, 2389-2401. Sur, J.H., Doster, A.R., Christian, J.S., Galeota, J.A., Wills, R.W., Zimmerman, J.J., Osorio, F.A., 1997. Porcine reproductive and respiratory syndrome virus replicates in testicular germ cells, alters spermatogenesis, and induces germ cell death by apoptosis. J Virol 71, 9170-9179. Takikawa, N., Kobayashi, S., Ide, S., Yamane, Y., Tanaka, Y., Yamagishi, H., 1996. Detection of antibodies against porcine reproductive and respiratory syndrome (PRRS) virus in swine sera by enzyme-linked immunosorbent assay. J Vet Med Sci 58, 355-357. Thanawongnuwech, R., Halbur, P.G., Andrews, J.J., 1997. Immunohistochemical detection of porcine reproductive and respiratory syndrome virus antigen in neurovascular lesions. J Vet Diagn Invest 9, 334-337. Tian, K., Yu, X., Zhao, T., Feng, Y., Cao, Z., Wang, C., Hu, Y., Chen, X., Hu, D., Tian, X., Liu, D., Zhang, S., Deng, X., Ding, Y., Yang, L., Zhang, Y., Xiao, H., Qiao, M., Wang, B., Hou, L., Wang, X., Yang, X., Kang, L., Sun, M., Jin, P., Wang, S., Kitamura, Y., Yan, J., Gao, G.F., 2007. Emergence of fatal PRRSV variants: unparalleled outbreaks of atypical PRRS in China and molecular dissection of the unique hallmark. PloS one 2, e526. Tian, X., Lu, G., Gao, F., Peng, H., Feng, Y., Ma, G., Bartlam, M., Tian, K., Yan, J., Hilgenfeld, R., Gao, G.F., 2009. Structure and cleavage specificity of the chymotrypsin-like serine protease (3CLSP/nsp4) of Porcine Reproductive and Respiratory Syndrome Virus (PRRSV). J Mol Biol 392, 977-993. Tiwari, S., Mishra, D.K., Roy, S., Singh, A., Singh, P.K., Tuli, R., 2009. High level expression of a functionally active cholera toxin B: rabies glycoprotein fusion protein in tobacco seeds. Plant Cell Rep 28, 1827-1836. Van Breedam, W., Van Gorp, H., Zhang, J.Q., Crocker, P.R., Delputte, P.L., Nauwynck, H.J., 2010. The M/GP(5) glycoprotein complex of porcine reproductive and respiratory syndrome virus binds the sialoadhesin receptor in a sialic acid-dependent manner. PLoS pathogens 6, e1000730. Van Den Born, E., Gultyaev, A.P., Snijder, E.J., 2004. Secondary structure and function of the 5'-proximal region of the equine arteritis virus RNA genome. RNA (New York, N.Y.) 10, 424-437. Van den Heuvel, M.M., Tensen, C.P., van As, J.H., Van den Berg, T.K., Fluitsma, D.M., Dijkstra, C.D., Dopp, E.A., Droste, A., Van Gaalen, F.A., Sorg, C., Hogger, P., Beelen, R.H., 1999. Regulation of CD 163 on human macrophages: cross-linking of CD163 induces signaling and activation. J Leukoc Biol 66, 858-866. Van Gorp, H., Van Breedam, W., Delputte, P.L., Nauwynck, H.J., 2008. Sialoadhesin and CD163 join forces during entry of the porcine reproductive and respiratory syndrome virus. J Gen Virol 89, 2943-2953. Van Gorp, H., Van Breedam, W., Delputte, P.L., Nauwynck, H.J., 2009. The porcine reproductive and respiratory syndrome virus requires trafficking through CD163-positive early endosomes, but not late endosomes, for productive infection. Arch Virol 154, 1939-1943. Vanderheijden, N., Delputte, P.L., Favoreel, H.W., Vandekerckhove, J., Van Damme, J., van Woensel, P.A., Nauwynck, H.J., 2003. Involvement of sialoadhesin in entry of porcine reproductive and respiratory syndrome virus into porcine alveolar macrophages. J Virol 77, 8207-8215. Wensvoort, G., Terpstra, C., Pol, J.M., ter Laak, E.A., Bloemraad, M., de Kluyver, E.P., Kragten, C., van Buiten, L., den Besten, A., Wagenaar, F., et al., 1991. Mystery swine disease in The Netherlands: the isolation of Lelystad virus. Vet Q 13, 121-130. Wills, R.W., Zimmerman, J.J., Yoon, K.J., Swenson, S.L., Hoffman, L.J., McGinley, M.J., Hill, H.T., Platt, K.B., 1997. Porcine reproductive and respiratory syndrome virus: routes of excretion. Vet Microbiol 57, 69-81. Wissink, E.H., Kroese, M.V., van Wijk, H.A., Rijsewijk, F.A., Meulenberg, J.J., Rottier, P.J., 2005. Envelope protein requirements for the assembly of infectious virions of porcine reproductive and respiratory syndrome virus. J Virol 79, 12495-12506. Yang, L., Frey, M.L., Yoon, K.J., Zimmerman, J.J., Platt, K.B., 2000. Categorization of North American porcine reproductive and respiratory syndrome viruses: epitopic profiles of the N, M, GP5 and GP3 proteins and susceptibility to neutralization. Arch Virol 145, 1599-1619. Yoon, I.J., Joo, H.S., Christianson, W.T., Kim, H.S., Collins, J.E., Morrison, R.B., Dial, G.D., 1992. An indirect fluorescent antibody test for the detection of antibody to swine infertility and respiratory syndrome virus in swine sera. J Vet Diagn Invest 4, 144-147. Yoon, I.J., Joo, H.S., Goyal, S.M., Molitor, T.W., 1994. A modified serum neutralization test for the detection of antibody to porcine reproductive and respiratory syndrome virus in swine sera. J Vet Diagn Invest 6, 289-292. Yoon, K.J., Wu, L.L., Zimmerman, J.J., Hill, H.T., Platt, K.B., 1996. Antibody-dependent enhancement (ADE) of porcine reproductive and respiratory syndrome virus (PRRSV) infection in pigs. Viral Immunol 9, 51-63. Yoon, K.J., Wu, L.L., Zimmerman, J.J., Platt, K.B., 1997. Field isolates of porcine reproductive and respiratory syndrome virus (PRRSV) vary in their susceptibility to antibody dependent enhancement (ADE) of infection. Vet Microbiol 55, 277-287. Yoon, K.J., Zimmerman, J.J., Swenson, S.L., McGinley, M.J., Eernisse, K.A., Brevik, A., Rhinehart, L.L., Frey, M.L., Hill, H.T., Platt, K.B., 1995. Characterization of the humoral immune response to porcine reproductive and respiratory syndrome (PRRS) virus infection. J Vet Diagn Invest 7, 305-312. Yuan, S., Murtaugh, M.P., Schumann, F.A., Mickelson, D., Faaberg, K.S., 2004. Characterization of heteroclite subgenomic RNAs associated with PRRSV infection. Virus Res 105, 75-87. Zhou, Y.J., Hao, X.F., Tian, Z.J., Tong, G.Z., Yoo, D., An, T.Q., Zhou, T., Li, G.X., Qiu, H.J., Wei, T.C., Yuan, X.F., 2008. Highly virulent porcine reproductive and respiratory syndrome virus emerged in China. Transbound Emerg Dis 55, 152-164. Ziebuhr, J., Snijder, E.J., Gorbalenya, A.E., 2000. Virus-encoded proteinases and proteolytic processing in the Nidovirales. J Gen Virol 81, 853-879. Zimmerman, J.J., Yoon, K.J., Pirtle, E.C., Wills, R.W., Sanderson, T.J., McGinley, M.J., 1997. Studies of porcine reproductive and respiratory syndrome (PRRS) virus infection in avian species. Vet Microbiol 55, 329-336. Zuckermann, F.A., Garcia, E.A., Luque, I.D., Christopher-Hennings, J., Doster, A., Brito, M., Osorio, F., 2007. Assessment of the efficacy of commercial porcine reproductive and respiratory syndrome virus (PRRSV) vaccines based on measurement of serologic response, frequency of gamma-IFN-producing cells and virological parameters of protection upon challenge. Vet Microbiol 123, 69-85. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62536 | - |
dc.description.abstract | 豬生殖和呼吸綜合症病毒(porcine reproductive and respiratory syndrome Virus, PRRSV) 為一正向單股RNA病毒,屬於動脈病毒科(Arteriviridae)巢狀病毒目(Nidovirales)。自1980年代此病毒引起的疾病,便成為全世界養豬產業危害最嚴重的疾病之一,病毒感染造成母豬繁殖障礙及生長豬隻呼吸系統疾病。PRRSV疫苗早在1990年代中期即開始使用,但十多年來,無論死毒疫苗或馴化活毒疫苗,均無法有效預防或控制PRRS。由於PRRSV在宿主誘發之免疫反應相當複雜,且具迴避宿主免疫系統之機制,使得發展此病毒之疫苗具有相當困難性。之前的基因轉殖植物口服次單位疫苗GP5-LTB (Escherichia coli heat-labile enterotoxin B subunit; 大腸桿菌熱溶解性次單位B融合蛋白)已能激發有效的免疫反應且有一定的保護效力,唯其中和抗體力價仍有改善空間。本實驗設計共同表現GP5-M及LTB之基因轉殖菸草口服次單位疫苗(LTB-M-GP5-T),並與商用MLV疫苗做比較,評估兩組疫苗於豬隻的安全性及免疫效力。將24頭5-6週齡PRRSV抗體陰性SPF豬隻分組,以兩週間隔進行三次GP5-M-LTB-T口服免疫,商用Ingelvac MLV疫苗組兩次肌肉注射免疫,於最後一次免疫後三週以5x105 TCID50 PRRSV MD001每頭動物給予2 ml攻毒進行動物實驗。本實驗檢測抗PRRSV抗體反應,以qrt-RT-PCR偵測病毒血症及唾液及組織病毒含量,並根據豬隻臨床生長表現(體重增長變化及平均日增重)評估疫苗安全性。本實驗結果顯示連續三次服用50公克劑量之菸草於豬隻生長表現並無影響。體液性免應反應結果顯示,GP5-M-LTB-T疫苗組在三次免疫中可偵測到血清PRRSV-specific IgG抗體反應並有逐漸上升趨勢,商用MLV疫苗組亦具有同樣的趨勢,兩組疫苗與控制組於統計上有顯著差異。GP5-M-LTB-T疫苗組於唾液有PRRSV-specific IgA抗體反應,但MLV商用疫苗組唾液無IgA抗體反應。GP5-M-LTB-T疫苗組組織病毒量普遍低於控制組且具有顯著差異,但是MLV商用疫苗組組織病毒含量具有相對更顯著的統計差異,然在病毒血症的部份,三組間並無統計顯著差異。總結本實驗結果,GP5-M-LTB-T疫苗可以啟動體液性及系統性免疫反應,基因轉殖菸草口服次單位疫苗及商用疫苗具有一定的保護效力但無法避免豬隻PRRSV感染。 | zh_TW |
dc.description.abstract | Porcine reproductive and respiratory syndrome virus (PRRSV) is a single-stranded positive RNA virus, which belongs to the family Arteriviridae and is classified in the order of Nidovirales. PRRS has been one of the most economically significant diseases of swine industry since 1980 and is characterized by reproductive failure in sows and respiratory distress in growing pigs. Although PRRSV modified live-attenuated vaccines (MLVs) and inactivated vaccines have been available for more than a decade, the efficacy of both vaccines is limited. The complicate evading immune mechanism by PRRSV makes it difficult to develop vaccine, both effective and safe. The objective of the present study was to investigate the immunogenicity and efficacy of a transgenic tobacco PRRS subunit vaccine containing GP5-M protein and Escherichia coli heat-labile enterotoxin-B subunit (LTB) after oral administration. In addition, Ingelvac MLV vaccine was included in the study. Twenty-four 5-to 6-week old pigs, seronegative for PRRSV, were randomly divided into 4 groups. Pigs of group one were given orally three consecutive doses of equal concentration of recombinant GP5-M protein expressed in the leaves of tobacco (GP5-M-LTB-T) at a 2-week interval. Pigs from the group were inoculated intramuscularly with 2 ml of Ingelvac PRRS MLV vaccine two times. The pigs were challenged with 2 ml 5x105 TCID50 PRRSV MD001 at 8 weeks post-initial immunization (WPI). The efficacy of the two different types of vaccines was evaluated based on clinical parameters, immune responses as well as post-challenge virological profiles. Serologic and saliva responses were measured by ELISA; viremia, saliva and tissue viral loads upon necropsy were measured by quantitative real time PCR. The results of changes in the bodyweights and daily weight gain revealed no statistical significance among groups, and no clinical signs were observed throughout the study. The levels of serum PRRSV ORF5-specific IgG in GP5-M-LTB-T and MLV groups elevated gradually after three or two consecutive immunization, respectively, and were significantly higher than that of the control group. The level of saliva PRRSV ORF5-specific IgA elevated gradually in GP5-M-LTB-T, while the MLV group didn’t demonstrate IgA. The levels of tissue viral load in GP5-M-LTB-T did have statiscally significant differences from control group, while the MLV group generally showed lower mean values than those of control group and GP5-M-LTB-T group. On the contrary, the results of viremia showed no significant differences among the three groups. In summary, the present study has demonstrated that GP5-M-LTB-T transgenic tobacco leaves could induce mucosal and systemic immune responses in pigs, while neither GP5-M-LTB-T nor MLV group could prevent pigs from PRRSV infection. The preliminary results support the foreseeable potential of subunit vaccine development. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T16:04:00Z (GMT). No. of bitstreams: 1 ntu-102-R00644006-1.pdf: 1386208 bytes, checksum: 1eb5adb0ef8981d7053091599b82ecd9 (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要.................................................................................................................... i
英文摘要.................................................................................................................... iii 目錄............................................................................................................................ vi 表次............................................................................................................................ viii 圖次............................................................................................................................ viii 第一章 序言........................................................................................................... 1 第二章 文獻回顧.................................................................................................... 3 第一節 豬生殖和呼吸綜合症病毒.................................................................. 3 1.1 PRRSV的病毒學分類........................................................................ 3 1.2 PRRSV的結構組成............................................................................ 3 1.3 PRRSV進入宿主細胞以及細胞內複製模式.................................... 5 1.4 PRRSV之致病機制與免疫機轉........................................................ 7 第二節 豬生殖和呼吸綜合症.......................................................................... 8 2.1 歷史背景與流行病學......................................................................... 8 2.2 臨床症狀與病理變化......................................................................... 9 2.3 體液性免疫反應................................................................................. 12 2.4 細胞性免疫反應................................................................................. 13 2.5 豬隻高熱症侯群................................................................................. 14 第三節 豬生殖和呼吸綜合症疫苗發展歷史與次單位疫苗之概述.............. 15 第三章 實驗設計與方法........................................................................................ 18 第一節 實驗設計.............................................................................................. 18 第二節 實驗材料.............................................................................................. 19 第三節 實驗方法.............................................................................................. 20 3.1 樣本採集與處理................................................................................. 20 3.2 酵素連結吸附免疫分析法檢測PRRSV ORF5-specific IgG……… 21 3.3 酵素連結吸附免疫分析法檢測PRRSV ORF7-specific IgG……… 22 3.4 酵素連結吸附免疫分析法檢測PRRSV ORF5-specific IgA……… 22 3.5 肉眼與組織病理學檢查..................................................................... 22 3.6 豬隻組織病毒含量............................................................................. 23 3.7 豬隻血清及唾液病毒含量................................................................ 24 3.8次單位疫苗之安全性試驗................................................................ 24 第四節 數據分析.............................................................................................. 25 第四章 結果……………………………………………………………………… 25 第一節 次單位疫苗之安全性試驗………………………………………… 25 第二節 體液性免疫反應…………………………………………………… 26 2.1 酵素連結吸附免疫分析法檢測PRRSV ORF5-specific IgG……… 26 2.2 酵素連結吸附免疫分析法檢測PRRSV ORF7-specific IgG……… 28 2.3 酵素連結吸附免疫分析法檢測PRRSV ORF5-specific IgA……… 29 第三節 疫苗保護效力分析………………………………………………….. 30 3.1血清病毒含量………………………………………………………. 30 3.2 唾液病毒含量………………………………………………………. 33 3.3 組織病毒含量………………………………………………………. 34 3.4 肉眼與組織病理學檢查…………………………………………….. 36 第五章 討論……………………………………………………………………… 38 第六章 參考文獻………………………………………………………………… 46 | |
dc.language.iso | zh-TW | |
dc.title | 豬生殖和呼吸道綜合症病毒GP5-M與大腸桿菌熱溶解性腸毒素次單位B(LTB)融合蛋白之基因轉殖菸草口服疫苗於豬隻之免疫效力評估 | zh_TW |
dc.title | Evaluation of the immunogenicity and efficacy of a transgenic tobacco plant expressing the recombinant fusion protein of GP5-M of porcine reproductive and respiratory syndrome virus and B subunit of Escherichia coli heat-labile enterotoxin in pig | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 龐飛(Victor Fei Pang),張志成 | |
dc.subject.keyword | 豬隻生殖和呼吸道症候症病毒,基因轉殖植物次單位疫苗,黏膜免疫,大腸桿菌熱溶解性次單位B融合蛋白,基因轉殖菸草, | zh_TW |
dc.subject.keyword | PRRSV,subunit vaccine,mucosal immunity,LTB,transgenic tobacco leaves, | en |
dc.relation.page | 61 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-06-28 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 分子暨比較病理生物學研究所 | zh_TW |
顯示於系所單位: | 分子暨比較病理生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 1.35 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。