Please use this identifier to cite or link to this item:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62279Full metadata record
| ???org.dspace.app.webui.jsptag.ItemTag.dcfield??? | Value | Language |
|---|---|---|
| dc.contributor.advisor | 林啟萬(Chii-Wann Lin) | |
| dc.contributor.author | Ling Ting | en |
| dc.contributor.author | 丁羚 | zh_TW |
| dc.date.accessioned | 2021-06-16T13:38:18Z | - |
| dc.date.available | 2023-12-31 | |
| dc.date.copyright | 2013-07-19 | |
| dc.date.issued | 2013 | |
| dc.date.submitted | 2013-07-16 | |
| dc.identifier.citation | [1] M. Tanihara, Y. Suzuki, E. Yamamoto, A. Noguchi, and Y. Mizushima, 'Sustained release of basic fibroblast growth factor and angiogenesis in a novel covalently crosslinked gel of heparin and alginate,' Journal of biomedical materials research, vol. 56, pp. 216-221, 2001.
[2] J. M. Anderson, A. Rodriguez, and D. T. Chang, 'Foreign body reaction to biomaterials,' Seminars in Immunology, vol. 20, pp. 86-100, 4// 2008. [3] J. M. Morais, F. Papadimitrakopoulos, and D. J. Burgess, 'Biomaterials/tissue interactions: possible solutions to overcome foreign body response,' The AAPS journal, vol. 12, pp. 188-196, 06/ 2010. [4] T.-C. Chen, 'Electrophysiolgical Study of Pulsed Radiofrequency Treatment in Neuropathic Pain Model and Behavior Study of Long-term Implantation,' Master, Graduate Institute of Biomedical Engineering, National Taiwan University, Taiwan, 2011. [5] J. E. Rosen and F. X. Gu, 'Surface Functionalization of Silica Nanoparticles with Cysteine: A Low-Fouling Zwitterionic Surface,' Langmuir, vol. 27, pp. 10507-10513, 2011/09/06 2011. [6] J. L. Dalsin, B. H. Hu, B. P. Lee, and P. B. Messersmith, 'Mussel adhesive protein mimetic polymers for the preparation of nonfouling surfaces,' Journal of the American Chemical Society, vol. 125, pp. 4253-4258, 04/ 2003. [7] M. Perretti and A. Ahluwalia, 'The microcirculation and inflammation: site of action for glucocorticoids,' Microcirculation (New York, N.Y. : 1994), vol. 7, pp. 147-161, 06/ 2000. [8] S. D. Patil, F. Papadimitrakopoulos, and D. J. Burgess, 'Dexamethasone-loaded poly (lactic-co-glycolic) acid microspheres/poly (vinyl alcohol) hydrogel composite coatings for inflammation control,' Diabetes technology & therapeutics, vol. 6, pp. 887-897, 2004. [9] S. D. Patil, F. Papadmitrakopoulos, and D. J. Burgess, 'Concurrent delivery of dexamethasone and VEGF for localized inflammation control and angiogenesis,' Journal of Controlled Release, vol. 117, pp. 68-79, 1/22/ 2007. [10] W. K. Ward, M. J. Quinn, M. D. Wood, K. L. Tiekotter, S. Pidikiti, and J. A. Gallagher, 'Vascularizing the tissue surrounding a model biosensor: how localized is the effect of a subcutaneous infusion of vascular endothelial growth factor (VEGF)?,' Biosensors and Bioelectronics, vol. 19, pp. 155-163, 11/30/ 2003. [11] A. Kedem, A. Perets, I. Gamlieli-Bonshtein, M. Dvir-Ginzberg, S. Mizrahi, and S. Cohen, 'Vascular endothelial growth factor-releasing scaffolds enhance vascularization and engraftment of hepatocytes transplanted on liver lobes,' Tissue Eng, vol. 11, pp. 715-722, //n 2005. [12] V. S. Polikov, P. A. Tresco, and W. M. Reichert, 'Response of brain tissue to chronically implanted neural electrodes,' J Neurosci Methods, vol. 148, pp. 1-18, Oct 15 2005. [13] X. Fan, L. Lin, J. L. Dalsin, and P. B. Messersmith, 'Biomimetic Anchor for Surface-Initiated Polymerization from Metal Substrates,' Journal of the American Chemical Society, vol. 127, pp. 15843-15847, 2005/11/01 2005. [14] P. Lin;, F. Gu;, and C.-W. Lin;, 'Non-Fouling Characteristics of Zwitterionic Cysteine Surface,' 2013. [15] V. Silin, H. Weetall, and D. J. Vanderah, 'SPR Studies of the Nonspecific Adsorption Kinetics of Human IgG and BSA on Gold Surfaces Modified by Self-Assembled Monolayers (SAMs),' Journal of Colloid and Interface Science, vol. 185, pp. 94-103, 1/1/ 1997. [16] L. A. Geddes and R. Roeder, 'Criteria for the selection of materials for implanted electrodes,' Annals of Biomedical Engineering, vol. 31, pp. 879-890, 2003. [17] J. Wu, F. G. Risalvato, F.-S. Ke, P. J. Pellechia, and X.-D. Zhou, 'Electrochemical Reduction of Carbon Dioxide I. Effects of the Electrolyte on the Selectivity and Activity with Sn Electrode,' Journal of The Electrochemical Society, vol. 159, pp. F353-F359, 2012. | |
| dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/62279 | - |
| dc.description.abstract | 目前許多植入式醫療器材都面臨到器材被組織包覆，影響效能的問題。此問題在植入式電刺激系統，例如心律調節器、周邊神經刺激器上尤為顯著。由於植入式電刺激系統必須藉由輸出電訊號得以發揮器材本身之功能，而組織在器材表面之包覆將提高器材與生物體交界之電阻抗，使器材無法發揮作用。本研究主要目的即為了解金電極經過半胱胺酸修飾後，各種表面性質之改善。半胱胺酸是一種具有硫醇鍵、帶負電的羧基(-COO-)以及帶正電氨基(-NH3+)的雙極性分子。金電極被靜置於半胱胺酸水溶液後，半胱胺酸之硫便會以自組裝(Self-assemble)之方式與金形成硫金鍵結，並在金電極形成低汙(Low-fouling)表面，有機會減少物質吸附。首先，我們透過表面電漿共振法檢測金電極對於抵抗血清蛋白吸附的能力，實驗發現，以0.1mM、1mM、10mM、50mM四種不同濃度半胱胺酸修飾的金電極，相較於未修飾的金電極，都有顯著良好的抗血清蛋白吸附效果，其中以濃度越低成效越好。接著，我們將金電極植入大鼠組織之間，於取出後已掃描式電子顯微鏡觀察發現，修飾後的電極表面能顯著減少免疫細胞貼附的數量，並能延緩免疫細胞的活化。最後，我們架設一系統對電極進行電化學量測，並以Randles cell等效電路法分析電極在修飾半胱胺酸以及接觸血清蛋白後其阻抗的變化。結果顯示半胱胺酸的修飾層能同時藉由減少電極表面阻值、增加表面電容值、抑制血清蛋白的吸附三種方式，達到抑制電極整體電阻抗上升的功效。本研究初步證實了半胱胺酸修飾層對金電極表面在生物相容性與電阻抗改良的幫助，提供了一個有機會應用在改善植入式刺激電極的良好方案。 | zh_TW |
| dc.description.provenance | Made available in DSpace on 2021-06-16T13:38:18Z (GMT). No. of bitstreams: 1 ntu-102-R00548026-1.pdf: 4698496 bytes, checksum: 2e32f58902e78e12716ea9ee171d2b39 (MD5) Previous issue date: 2013 | en |
| dc.description.tableofcontents | 口試委員審定書 I
謝辭 II 摘要 III Abstract IV 圖目錄 V 表目錄 VII 第一章 導論 1 1.1 植入式醫療器材 (Implantable medical device) 1 1.2 異物反應 (Foreign Body Response) 5 第二章 文獻探討 6 2.1 表面修飾 6 2.2 半胱胺酸表面修飾 8 2.3 表面電漿感測技術於非特異性吸附檢測 12 2.4 表面修飾之應用於抑制免疫細胞貼附 14 2.5 等效電路系統 (Equivalent Circuit) 17 第三章 實驗方法與設計 20 3.1 金電極製備 20 3.2 血清蛋白吸附量測 20 3.3 免疫細胞貼附量測 21 3.3.1手術前置作業 22 3.3.2手術流程 22 3.3.3掃描式電子顯微鏡觀察 22 3.4 電阻抗量測 22 第四章 實驗結果 24 4.1 血清蛋白吸附實驗結果 24 4.2 免疫細胞貼附實驗結果 25 4.3 電阻抗量測結果 29 第五章 總結 33 參考文獻 34 | |
| dc.language.iso | zh-TW | |
| dc.title | 具生物相容性與電阻抗性質改善之半胱胺酸表面修飾刺激電極 | zh_TW |
| dc.title | The L-Cysteine Modified Stimulating Electrode with Enhanced Properties of Bio-compatibility and Electrical Impedance | en |
| dc.type | Thesis | |
| dc.date.schoolyear | 101-2 | |
| dc.description.degree | 碩士 | |
| dc.contributor.oralexamcommittee | 施文彬,邱銘章,郭義松 | |
| dc.subject.keyword | 刺激電極,異物反應,表面修飾,半胱胺酸,電阻抗, | zh_TW |
| dc.subject.keyword | Stimulating electrode,Foreign Body Response,Surface Modification,Cysteine,Electrical impedance, | en |
| dc.relation.page | 35 | |
| dc.rights.note | 有償授權 | |
| dc.date.accepted | 2013-07-16 | |
| dc.contributor.author-college | 工學院 | zh_TW |
| dc.contributor.author-dept | 醫學工程學研究所 | zh_TW |
| Appears in Collections: | 醫學工程學研究所 | |
Files in This Item:
| File | Size | Format | |
|---|---|---|---|
| ntu-102-1.pdf Restricted Access | 4.59 MB | Adobe PDF |
Items in DSpace are protected by copyright, with all rights reserved, unless otherwise indicated.