痲瘋樹之微體繁殖

Abstract

本研究以栽植於高雄六龜、田寮的高油量品系，藉器官發生途徑建立痲瘋樹再生系統。 本實驗先以野外痲瘋樹之葉片為培殖體進行表面消毒試驗。將新鮮葉片經流水處理2 hr，先後以稀釋300倍之anticeptol solution與70%酒精超音波震盪30 sec，接著以4%的NaOCl添加1滴的Tween 20 界面活性劑並震璗20 min的消毒效果最好，無汙染率可達86%。 在器官形成部分：種子經表面消毒後，取出成熟胚置於MS培養基中，無汙染率高達98%，建立無菌苗系統，培養六週平均苗高可達3.99 cm。選用下胚軸、帶節莖段及葉片進行芽體誘導試驗。結果顯示：下胚軸效果最佳，在MS添加0.5 mg l-1 BA下平均可誘導芽體數2.83個，誘導長度1.70 cm。誘導出的芽體在MS添加0.5 mg l-1 BA培養基中進行芽體伸長試驗，四週後芽體平均可伸長0.69 cm。伸長之芽體在1/2 MS添加1 mg l-1之IBA的培養基中，發根率為20%。將發根之芽體移至滅菌過後的土壤中，在溫室中馴化，兩個月後存活率達到76.19%。

In this study, an efficient method for plant regeneration from a high-oil content strain(Liugui, Tianliao) of Jatropha curcas L. through organogenesis was developed. Leaves of field-grown J. curcas were treated with running water for 2 hours, soaked in 1/300(v/v) anticeptol solution and 70% ethanol solution in order, and treated with ultrasonic shaker for 30 seconds for each step, then soaked in 4% NaOCl solution with a drop of Tween 20 and shaking for 20 minutes and the leaves could get 86% non-contamination. After surface sterilization, mature embryos were placed in basal medium. The non-contamination rate was 98%. The most suitable medium for aseptic plantlets is MS medium and the length of plantlets could reach 3.99 cm in average. The best number of shoot buds(2.89) per explant and average length(1.70 cm) was observed when in vitro hypocotyl explants were placed in MS medium with 0.5 mg l-1 BA. The induced shoot buds were transferred to MS medium containing 0.5 mg l-1 BA for shoot elongation and 0.69 cm elongation was achieved after 4 weeks. The elongated shoots could be rooted on 1/2 MS with 1 mg l-1 IBA and the induction rate was 20%. The rooted plants could be outplanted in sterilized soil with 76.19% survival rate after 2 months.