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完整後設資料紀錄
DC 欄位 | 值 | 語言 |
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dc.contributor.advisor | 王汎熒 | |
dc.contributor.author | Yen-Chun Tung | en |
dc.contributor.author | 董彥君 | zh_TW |
dc.date.accessioned | 2021-06-16T13:19:27Z | - |
dc.date.available | 2018-07-31 | |
dc.date.copyright | 2013-07-31 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-26 | |
dc.identifier.citation | Beer, M., Reimann, I., Hoffmann, B., Depner, K., 2007. Novel marker vaccines against classical swine fever. Vaccine 25, 5665-5670.
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dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61935 | - |
dc.description.abstract | 猪瘟為世界衛生組織規範於陸生動物衛生法典中的傳染病之一,為一猪隻急性病毒性疾病,具高傳染性及高死亡率,以全身性出血為主癥。猪瘟的爆發往往造成重大的經濟損失。在亞洲地區,1.1,2.3和3.4等血清型主要在日本;在泰國以1.1和1.2病毒株為主,中國則是2.1型。在台灣野外分離的猪瘟病毒可依基因型區分為本土型與外來型。 2.1病毒株首先於 1994年被分離到並於 1996年開始散播,根據潘等人利用 Erns及 E2醣蛋白進行逆轉錄聚合酶鏈反應以及定序分析,發現 1996之後外來型病毒已完全取代本土型病毒,且並非從本土型病毒株的基因突變所造成。目前台灣大部分的研究著重在製作出適用於此兩型病毒之疫苗,以期能撲滅猪瘟。現行之疫苗為 Lapinized Philippines Coronel (LPC) 減毒疫苗,主要是將強毒株不斷通過兔子繼代馴化後,得到 LPC 疫苗用的減毒兔化猪瘟病毒株。在檢驗上,施打 LPC疫苗的猪隻在抗體檢驗上無法與已被猪瘟自然感染的猪隻作區別,亦無法產生對抗野外株病毒的抗體。有研究顯示,猪瘟病毒 E2醣蛋白在不同病毒株之間具有抗原專一性,本研究使用 LPC 疫苗,以及自 LPC 疫苗, TD/96,94.4 病毒株中表現之 E2醣蛋白進行豬隻免疫,在免疫完成後各自以強毒株,外來型及本土型進行攻毒,並在攻毒後利用病理肉眼診斷及免疫組織化學染色進行分析以及積分分級,亦採集新鮮組織製成乳劑後萃取核酸進行逆轉錄聚合酶鏈反應。由統計結果顯示E2醣蛋白疫苗可提供相等於LPC疫苗之保護力。而在組織分布的部分,抗原主要呈現在腎臟的遠曲小管及腎絲球體內的微血管內皮、脾臟的 B細胞區域、淋巴結內淋巴濾泡以及肺臟的內皮細胞上。在強毒株感染的組織中(陽性對照),皆呈現強陽性(+++),而在有免疫的實驗組,E2醣蛋白免疫的組別皆呈現陰性,而使用LPC減毒疫苗免疫之組織中仍殘存較弱的陽性訊號(+)。因此本研究認為LPC減毒疫苗株會保存在於淋巴組織以及肺臟上皮,在病理檢驗時易造成混淆,而 E2次單位疫苗不僅能提供相等於LPC減毒疫苗的保護力,更能降低檢驗上的干擾。 | zh_TW |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:19:27Z (GMT). No. of bitstreams: 1 ntu-102-R00644007-1.pdf: 2094142 bytes, checksum: f2ae9f6b1800b91416c2757d06d1681d (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 中文摘要 I
Abstract III Contents V List of Tables VII List of Figures VIII List of Appendices IX Chapter Ⅰ Introduction 1 Chapter Ⅱ Literature review 3 2.1 Classical swine fever 3 2.1.1 History 3 2.1.2 Morphology, genomic organization and expression 3 2.1.3 Pathogenesis 4 2.1.4 Clinical signs 5 2.1.5 Lesions 6 2.1.6 Current situation in Taiwan 7 2.2 Vaccines for classical swine fever 7 2.2.1 Live-attenuated vaccine 7 2.2.2 E2 subunit vaccine 8 Chapter Ⅲ Aim of study 10 Chapter Ⅳ Materials and methods 11 4.1 Sample preparation 11 4.1.1 Positive control (ALD challenged) samples 11 4.1.2 Experimental samples 11 4.2 Animal tissues and sampling for histopathology 12 4.3 Immunohistochemistry 12 4.3.1 Antibodies 12 4.3.2 Classical indirect immunohistochemistry (IHC) 13 4.3.2 Scoring of IHC staining results 13 4.4 Molecular analysis 14 4.4.1 RNA extraction 14 4.4.2 Primer selection 15 4.4.3 One-step reverse transcription polymerase chain reaction (RT-PCR) 16 4.4.4 Gel electrophoresis 16 Chapter Ⅴ Results 17 5.1 Gross pathological examinations of pigs 17 5.2 Histopathological examination of IHC stained tissue sections 18 5.2.1 Quantification of IHC scores of tissue section 19 5.2.2 Analysis of IHC Scores 19 5.3 Results of RT-PCR assays 20 5.3.1 G3PDH as internal control for CSFV 20 5.3.2 Results of RT-PCR assays 21 Chapter Ⅵ Discussion and conclusion 22 Tables 25 Figures 28 Appendices 34 References 41 | |
dc.language.iso | en | |
dc.title | 豬瘟E2醣蛋白免疫及攻毒後病毒抗原在組織間分布之情形 | zh_TW |
dc.title | The effect of E2 glycoprotein vaccination on the distribution of classical swine fever virus antigen after challenge | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 張本恆,張伯俊,張家宜,李璠 | |
dc.subject.keyword | 豬瘟,E2, | zh_TW |
dc.subject.keyword | classical swine fever,E2, | en |
dc.relation.page | 46 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-07-26 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 分子暨比較病理生物學研究所 | zh_TW |
顯示於系所單位: | 分子暨比較病理生物學研究所 |
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