請用此 Handle URI 來引用此文件:
http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61911
完整後設資料紀錄
DC 欄位 | 值 | 語言 |
---|---|---|
dc.contributor.advisor | 張本恆 | |
dc.contributor.author | I-Wen Chen | en |
dc.contributor.author | 陳怡彣 | zh_TW |
dc.date.accessioned | 2021-06-16T13:18:22Z | - |
dc.date.available | 2015-07-30 | |
dc.date.copyright | 2013-07-30 | |
dc.date.issued | 2013 | |
dc.date.submitted | 2013-07-29 | |
dc.identifier.citation | [1] 黃印堯等。一起毀滅性鮑魚病毒病的調查。福建畜牧獸醫 21:4-5,1999。
[2] 黃印堯等。九孔鮑魚球狀病毒病的診斷和防治報告。福建畜牧獸醫,2000。 [3] Arzul I, Nicolas JL, Davison AJ, Renault T. French scallops: a new host for ostreid herpesvirus-1. Virol 290: 342-349, 2001. [4] Arzul I, Renault T, Lipart C, Davison AJ. Evidence for interspecies transmission of oyster herpesvirus in marine bivalves. J Gen Virol 82: 865-870, 2001. [5] Batista FM, Arzul I, Pepin JF, Ruano F, Friedman CS, Boudry P, Renault T. Detection of ostreid herpesvirus 1 DNA by PCR in bivalve molluscs: a critical review. J Virol Methods 139: 1-11, 2007. [6] Bercovier H, Fishman Y, Nahary R, Sinai S, Zlotkin A, Eyngor M, Gilad O, Eldar A, Hedrick RP. Cloning of the koi herpesvirus (KHV) gene encoding thymidine kinase and its use for a highly sensitive PCR based diagnosis. BMC Microbiol 5: 13, 2005. [7] Buongiorno-Nardelli M and Amaldi F. Autoradiographic detection of molecular hybrids between rRNA and DNA in tissue sections. Nature: 225, 946-948, 1970. [8] Chang PH, Kuo ST, Lai SH, Yang HS, Ting YY, Hsu CL, Chen HC. Herpes-like virus infection causing mortality of cultured abalone Haliotis diversicolor supertexta in Taiwan. Dis Aquat Org 65: 23-27, 2005. [9] Chen MH, Kuo ST, Renault T, Friedman CS, Chang PH. Development of a polymerase chain reaction for the detection of abalone herpesvirus infection based on the DNA polymerase gene. J Virol Methods 185:1-6, 2012. [10] Comps M and Cochennec N. A herpes-like virus from the European oyster Ostrea edulis L. J Invertebr Pathol 62: 201-203, 1993. [11] Corbeil S, Colling A, Williams LM, Wong FY, Savin K, Warner S, Murdoch B, Cogan NO, Sawbridge TI, Fegan M, Mohammad I, Sunarto A, Handlinger J, Pyecroft S, Douglas M, Changs PH, Crane MS. Development and validation of a TaqMan PCR assay for the Australian abalone herpes-like virus. Dis Aquat Organ 92: 1-10, 2010. [12] Davison AJ, Trus BL, Cheng N, Steven AC, Watson MS, Cunningham C, Le Deuff RM, Renault T. A novel class of herpesvirus with bivalve hosts. J Gen Virol 86: 41-53, 2005. [13] du Sart D and Choo KHA. (1996). The technique of in situ hybridization: principles and applications. In Repley R and Walker JM (Eds.), Molecular Biomethods Handbook (pp. 697-720). Totowa, NJ, Humana Press. [14] Farley CA, Banfield WG, Kasnic G Jr, Foster WS. Oyster herpes-type virus. Science 178: 759-760, 1972. [15] Gall JG and Pardue ML: Formation and detection of RNA-DNA hybrid molecules in cytological preparation. Proc Natl Acad Sci USA 63:378–383, 1969. [16] Harada T, Okazaki N, Otoishi Y, Hayakawa Y, Kubota SS. Tumors in Nervous tissues of abalones, Nordotis discus. J Invertebr Pathol 62: 257-261, 1993. [17] Hine PM. Trends in research on diseases of bivalve molluscs. Bull Eur Assoc Fish Pathol 17: 180-183, 1997. [18] Hine PM and Thorne T. Replication of herpes-like viruses in haemocytes of adult flat oysters Ostrea angasi: an ultrastructural study. Dis Aquat Organ 29: 189-196, 1997. [19] Hine PM, Wesney B, Besant P. Replication of a herpeslike virus in larvae of the flat oyster Tiostrea chilensis at ambient temperatures. Dis Aquat Organ 32, 161-171, 1998. [20] Hine PM, Wesney B, Hay BE. Herpesvirus associated with mortalities among hatchery-reared larval Pacific oysters Crassostrea gigas. Dis Aquat Organ 12: 135-142, 1992. [21] Hooper C, Hardy-Smith P, Handlinger J. Ganglioneuritis causing high mortalities in farmed Australian abalone (Haliotis laevigata and Haliotis rubra). Aust Vet J 85: 188-193, 2007. [22] Jin L and Lloyd RV. In situ hybridization: methods and applications. J Clin Lab Anal 11: 2-9, 1997. [23] Le Deuff RM and Renault T. Purification and partial genome characterization of a herpes-like virus infecting the Japanese oyster, Crassostrea gigas. J Gen Virol 80: 1317-1322, 1999. [24] Lipart C and Renault T. Herpes-like virus detection in infected Crassostrea gigas spat using DIG-labelled probes. J Virol Meth 101:1-10, 2002. [25] McGeoch DJ, Rixon FJ, Davison AJ. Topics in herpesvirus genomics and evolution. Virus Res 117: 90-104, 2006. [26] Minson AC, Davison A, Eberle R, Desrosiers RC, Fleckenstein B, McGeoch DJ, Pellett PE, Roizman B, Studdert MJ. (2000). Family Herpesviridae. In van Regenmortel MHV, Fauquet CM, Bishop DHL, Carstens EB, Estes MK, Lemon SM, Maniloff J, Mayo MA, McGeoch DJ, Pringle CR, Wickner RB (Eds.), Virus Taxonomy, Seventh Report of the International Committee on Taxonomy of Viruses (pp. 203-225). San Diego: Academic Press. [27] Nakatsugawa T, Nagai T, Hiya K, Nishizawa T, Muroga K. A virus isolated from juvenile Japanese black abalone Nordotis discus discus affected with amyotrophia. Dis Aquat Organ 36: 159-161, 1999. [28] Nicolas JL, Comps M, Cochennec N. Herpes-like virus infecting Pacific-oyster larvae, Crassostrea gigas. Bull Eur Assoc Fish Pathol 12: 11-13, 1992. [29] Otsu R and Sasaki K. Virus-like partivles detected from juvenile abalone ,Nordotis discus discus, reared with an epizootic fatal wasting disease. J Invertebr Pathol 70: 167-168, 1997. [30] Renault T. Herpes virus infecting marine molluscs. Sci-Topics, Elsevier, 11 August, 2008. www.scitopics.com/Herpes_virus_infecting_marine_molluscs.html. [31] Renault T and Arzul I. Herpes-like virus infections in hatchery-reared bivalve larvae in Europe: specific viral DNA detection by PCR. J Fish Dis 24: 161-167, 2001. [32] Renault T, Cochennec N, Le Deuff RM, Chollet B. Herpes-like virus infecting Japanese oyster, Crassostrea gigas, spat. Bull Eur Assoc Fish Pathol 14: 64-66, 1994. [33] Renault T, Le Deuff RM, Chollet B, Cochennec N, Ge’rard A. Concomitant herpes-like virus infections in hatchery-reared larvae and nursery-cultured spat Crassostrea gigas and Ostrea edulis. Dis Aquat Organ 42: 173-183, 2000. [34] Renault T, Le Deuff RM, Cochennec N, Chollet B, Maffart P. Herpes-like viruses associated with high mortality levels in larvae and spat of Pacific oysters, Crassostrea gigas: a comparative study, the thermal effects on virus detection in hatchery-reared larvae, reproduction of the disease in axenic larvae. Vet Res 26: 539-543, 1995. [35] Renault T, Le Deuff RM, Cochennec N, Maffart P. Herpesviruses associated with mortalities among Pacific oyster, Crassostrea gigas, in France-comparative study. Rev Med Vet 145: 735-742, 1994. [36] Renault T, Le Deuff RM, Lipart C, Delsert D. Development of a PCR procedure for the detection of a herpes-like virus infecting oysters in France. J Virol Methods 88: 41-50, 2000. [37] Renault T, Lipart C, Arzul I. A herpes-like virus infects a non-ostreid bivalve species: virus replication in Ruditapes philippinarum larvae. Dis Aquat Organ 45: 1-7, 2001. [38] Segarra A, Pepin JF, Arzul I, Morga B, Faury N, Renault T. Detection and description of a particular Ostreid herpesvirus 1 genotype associated with massive mortality outbreaks of Pacific oysters, Crassostrea gigas, in France in 2008. Virus Res 153: 92-99, 2010. [39] Tan J, Lancaster M, Hyatt A, van Driel R, Wong F, Warner S. Purification of a herpes-like virus from abalone, Haliotis spp., with ganglioneuritis and detection by transmission electron microscopy. J Virol Methods 149: 338-341, 2008. [40] Wang J, Su YQ, Zhang JN, Huang Y, Zhang ZX, Yan QP,Wang DX. Spring explosive epidemic disease of abalone in Dongshan District. J Xiamen Univ 38: 641-644, 1999. [41] Zhang ZX, Wan J, Su YQ, Yan QP, Chi XC, Zhou HM, Zhou YC. Pathogeny and histopathology of the epidemic disease in Haliotis diversicolor supertexta. J Xiamen Univ 40: 949-956, 2001 [42] Zhuang J, Cai G, Lin Q, Wu Z, Xie L. A bacteriophage-related chimeric marine virus infecting abalone. PLoS One 5, e13850, 2010. | |
dc.identifier.uri | http://tdr.lib.ntu.edu.tw/jspui/handle/123456789/61911 | - |
dc.description.abstract | 2003年冬天,台灣養殖九孔鮑因鮑魚皰疹病毒(abalone herpesvirus, AbHV)的感染引起大量死亡。組織病理學顯示,AbHV主要感染神經系統,造成神經元的壞死並引發神經節神經炎。本實驗的目的為利用原位雜交技術確認鮑魚皰疹病毒(AbHV)於感染宿主體內的分佈位置,以探討其發病機制。在本實驗中,原位雜交的結果顯示AbHV的陽性訊號主要集中在神經節的神經元及血淋巴細胞,而對照組則無特異性結合,與先前研究資料相符,確認AbHV為一嗜神經病毒。
近年來在台灣懷疑有另一種鮑魚的病毒性疾病。此一未知病毒(AbV2)可利用由皰疹病毒特異的保留區段所設計的引子增幅出特定長度的基因片段,將此一特定長度的基因片段定序,結果顯示該病毒與其他種類的皰疹病毒具有低度的基因相似性。依先前的原位雜交技術流程進行染色,結果顯示AbV2的陽性訊號侷限於血球細胞,為一親血球性的病毒。將AbV2的探針回溯性使用於AbHV陽性的病例中,結果在血球細胞中也可見到陽性訊號,表示在AbHV發病疫情當時,AbV2可能已經存在於台灣的養殖九孔鮑。 | zh_TW |
dc.description.abstract | Aabalone herpesvirus (AbHV) infection of cultured abalone Haliotis diversicolor supertexta has induced high mortality in Taiwan in 2003. The histopathologic study has revealed AbHV is neurotropic and has caused ganglioneuritis in moribund abalone. In this study, an in situ hybridization protocol was developed to detect AbHV gene distribution within the host to verify its pathogenesis. This study confirmed that the expression pattern of AbHV was restricted to neurons as described previously by light and transmission electron microscopy, and confirmed this is a neurotropic virus.
Another abalone virus disease is suspected in Taiwan recently. This undetermined virus (AbV2) can be amplified gene fragment in specific length by primer designed from the conserved region of herpesvirus and further sequencing revealed this virus is belonged to members of the Herpesvirales, however, the identity was low. ISH protocol is followed by aforementioned one. The result revealed AbV2 was restricted to hemocytes and suggested this is a hemocytropic virus. Retrospective study revealed AbV2 may have already existed in the farms before the first outbreak of AbHV. | en |
dc.description.provenance | Made available in DSpace on 2021-06-16T13:18:22Z (GMT). No. of bitstreams: 1 ntu-102-R00644010-1.pdf: 1330145 bytes, checksum: 26fd4dbb8cffc7cc7962f77cdac4e55d (MD5) Previous issue date: 2013 | en |
dc.description.tableofcontents | 第一章 前言 1
第二章 文獻討論 3 第一節 鮑魚的病毒性疾病 3 2.1.1 肌萎縮病 3 2.1.2 球狀病毒與弧菌混合感染 3 2.1.3 親神經性的皰疹病毒 4 2.1.4 鮑魚嗜菌體相關病毒 5 第二節 牡蠣皰疹病毒研究的發展 6 第三節 原位雜交技術 7 2.3.1 基本原理與方法 8 第三章 材料與方法 11 第一節 實驗設計 11 第二節 材料來源 11 第三節 型態病理學檢查 11 3.3.1 組織病理學檢查 11 3.3.2 原位雜交技術 14 第四節 分子病理學檢查 17 3.4.1 病毒核酸的萃取 17 3.4.2 PCR 19 3.4.3 DNA步移法 20 第四章 實驗結果 26 第一節 分子生物學檢查結果 26 4.1.1 第二型九孔鮑病毒(AbV2)探討 26 4.1.2 第二型九孔鮑病毒(AbV2)的基因 26 第二節 形態病理學檢查結果 27 4.2.1 鮑魚皰疹病毒(AbHV)的原位雜交染色結果 27 4.2.2 第二型九孔鮑病毒(AbV2)的原位雜交染色結果 27 4.2.3 兩型鮑魚病毒的原位雜交染色結果比較 28 第五章 討論 38 第一節 分子生物學結果探討 38 第二節 原位雜交染色結果探討 39 5.2.1 原位雜交技術於鮑魚皰疹病毒的應用 39 5.2.2 原位雜交技術於兩型鮑魚病毒的結果比較 40 第三節 結論 41 第四節 未來展望 41 REFERENCES 42 | |
dc.language.iso | zh-TW | |
dc.title | 以原位雜交技術應用於九孔鮑皰疹病毒之研究 | zh_TW |
dc.title | Development of in Situ Hybridization in the Study of Abalone Herpes Virus Diagnosis | en |
dc.type | Thesis | |
dc.date.schoolyear | 101-2 | |
dc.description.degree | 碩士 | |
dc.contributor.oralexamcommittee | 王汎熒,陳三多,邱品文 | |
dc.subject.keyword | 九孔鮑,皰疹病毒,原位雜交技術,病理,親神經性,親血球性, | zh_TW |
dc.subject.keyword | Aabalone,Herpesvirus,in situ hybridization,Pathology,Neurotropic,Hemocytropic, | en |
dc.relation.page | 48 | |
dc.rights.note | 有償授權 | |
dc.date.accepted | 2013-07-29 | |
dc.contributor.author-college | 獸醫專業學院 | zh_TW |
dc.contributor.author-dept | 分子暨比較病理生物學研究所 | zh_TW |
顯示於系所單位: | 分子暨比較病理生物學研究所 |
文件中的檔案:
檔案 | 大小 | 格式 | |
---|---|---|---|
ntu-102-1.pdf 目前未授權公開取用 | 1.3 MB | Adobe PDF |
系統中的文件,除了特別指名其著作權條款之外,均受到著作權保護,並且保留所有的權利。